Scn10a (NM_001205321) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	Myc-DDK
Synonyms	Nav1.8; PN3; SNS
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MR231896 representing NM_001205321 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGTTCCCCTTTGGGTCCGTGGGAACTACCAACTTCAGACGGTTCACTCCAGAGTCGCTGGCAGAGA TCGAGAAGCAGATCGCTGCCCACCGCGCCGCCAAGAAGGGCAGACCTAAGCAAAGAGGACAGAAGGACAA GAGTGAGAAGCCCAGGCCTCAGTTGGACTTGAAGGCCTGTAACCAGCTGCCCAGGTTCTATGGCGAGCTC CCAGCAGAGCTGGTCGGGGAGCCCCTGGAGGACCTGGATCCTTTCTACAGCACACACCGGACATTCATAG TGTTGGATAAAAGCAGGACCATTTCCAGATTCAGTGCCACTTGGGCTCTGTGGCTCTTCAGTCCCTTCAA CCTGATCAGAAGAACAGCCATCAAAGTGTCCGTCCACTCCTGGTTCTCCATATTTATCACTGTCACTATT TTGGTCAACTGTGTGTGCATGACCCGAACTGATCTTCCAGAGAAACTCGAGTATGCCTTCACTGTTGTTT ACACCTTCGAGGCTCTGATAAAGATACTGGCAAGAGGGTTTTGTCTAAATGAATTCACTTATCTTCGAGA TCCCTGGAACTGGCTGGACTTCAGTGTCATTACCCTGGCGTATGTGGGTGCAGCGATAGACCTCCGAGGA ATCTCAGGCCTGCGGACATTCCGAGTTCTCAGGGCCCTGAAGACTGTTTCTGTGATCCCAGGACTGAAGG TCATCGTGGGAGCCCTGATCCACTCAGTGAGGAAGCTGGCCGACGTGACCATCCTCACAGTCTTCTGCCT GAGTGTCTTTGCCTTGGTGGGCCTGCAGCTCTTCAAGGGGAACCTCAAGAATAAATGCATTAAGAACGGC ACAGATCCGCACAAGGCTGACAATCTCTCATCTGAAATGGCAGGAGACATCTTCATCAAGCCCGGTACTA CGGATCCCTTGTTGTGTGGCAATGGATCTGATGCTGGCCACTGCCCTAATGATTATGTCTGCCGGAAAAC TTCTGACAACCCGGATTTTAACTACACCAGCTTTGATTCCTTCGCGTGGGCGTTCCTCTCACTGTTCCGT CTCATGACGCAGGACTCCTGGGAACGGCTGTACCAGCAGACACTCCGGGCTTCCGGGAAAATGTACATGG TCTTTTTTGTGCTGGTCATCTTCCTTGGATCATTCTACCTGGTCAATTTGATCTTGGCTGTGGTCACCAT GGCATATGAGGAACAGAGCCAGGCAACAATTGCAGAAATCGAAGCCAAGGAGAAGAAGTTCAAGGAAGCC CTCGAGGTGCTGCAGAAAGAACAGGAGGTGCTGGCAGCGCTGGGCATTGACACAACCTCGCTCTATTCCC ACAACGGATCACCCTTAGCCCCCAAAAACGCCAATGAGAGAAGACCCAGGGTGAAATCAAGGATGTCAGA AGGCTCGACAGATGACAACAGATCACTACAATCCGACCCTTACAACCAGCGCAGGATGTCTTTCCTAGGC CTTTCTTCTGGAAGACGCAGGGCTAGCCACAGCAGTGTGTTCCACTTCCGAGCACCCAGCCAAGACGTCT CATTTCCTGATGGGATCTTGGATGACGGGGTCTTTCATGGAGATCAGGAAAGCCGTCGAAGTTCCATATT GCTGGGCAGGGGTGCCGGGCAGGCAGGTCCTCTCCCCAGGAGTCCACTGCCTCAGTCCCCCAACCCTGGC CCTAGACGTGGAGAAGAGGGACAGCGTGGAGTGCCCACTGGTGAGCTTGCCACTGGAGCGCCTGAAGGCC CGGCACTCGATGCTGCAGGACAGAAGAACTTCCTGTCTGCAGACTACTTGAATGAACCTTTCCGAGCACA GAGGGCAATGAGTGTTGTCAGTATTATGACTTCTGTCATTGAGGAGCTGGAAGAGTCTAAGCTGAAGTGC CCACCCTGCTTGATCAGCTTAGCCCAGAAGTACCTGATATGGGAGTGCTGCCCCAAGTGGAAGAAATTCA AGATGGTGCTCTTCGAGCTGGTGACTGACCCCTTCGCAGAGCTCACCATCACCCTGTGCATTGTGGTGAA TACCGTCTTCATGGCCATGGAACACTACCCCATGACTGATGCCTTCGATGCCATGCTCCAAGCCGGCAAC ATTGTCTTTACTGTGTTTTTTACAATGGAGATGGCCTTCAAGATCATTGCCTTCGACCCCTACTACTACT TCCAGAAGAAGTGGAACATCTTCGACTGTGTCATCGTCACCGTGAGCCTGCTGGAGCTGAGCACATCCAA GAAGGGCAGCTTGTCTGTGCTCCGCACCTTCCGCTTGCTTCGGGTCTTCAAGCTGGCCAAGTCCTGGCCC ACCCTGAACATGCTCATCAAGATCATCGGGAACTCTGTGGGGGCCCTGGGCAACCTGACCTTCATCCTGG CCATCATCGTCTTTATCTTCGCCCTGGTGGGAAAGCAGCTCCTCTCAGAGAACTATGGGTGCCGCAGGGA TGGCATCTCCGTGTGGAATGGTGAGAGGCTGCGCTGGCACATGTGTGACTTCTTCCATTCCTTCCTCGTC GTCTTCCGGATCCTCTGCGGGGAGTGGATCGAGAACATGTGGGTCTGCATGGAGGTCAGCCAGGACTACA TCTGCCTCACCCTCTTCTTGACAGTGATGGTGCTAGGCAACCTGGTGGTGCTCAACCTATTCATCGCTTT ACTGCTGAACTCCTTCAGTGCGGACAACCTCACAGCCCCAGAGGATGACGGGGAGGTGAACAACTTGCAG GTAGCACTGGCCCGGATTCAGGTATTTGGCCATCGGGCCAGTCGGGCCATTACCAGTTACATCAGAAGCC ACTGCCGGCTCCGCTGGCCCAAGGTGGAGACCCAGCTGGGGATGAAACCCCCACTCACCAGCTGCAAAGC TGAGAACCACATTGCTACTGATGCTGTCAATGCTGCAGTGGGGAACCTGGCAAAGCCAGCTCTTGGTGGC CCCAAGGAGAACCACGGGGACTTCATCACTGATCCTAACGTGTGGGTCTCTGTGCCCATTGCTGAGGGGG AGTCCGACCTTGATGAGCTCGAGGAAGATGTGGAGCATGCTTCTCAGAGCTCCTGGCAGGAAGAGAGCCC CAAAGGGCAGCAGGAGCTGCTGCAGCAAGTCCAAAAGTGTGAAGATCACCAGGCAGCCCGAAGCCCACCC TCCGGGATGTCCTCTGAGGACCTGGCTCCATACCTGGGGGAGAGATGGCAGAGGGAGGAGAGCCCTCGGG TCCCTGCCGAGGGAGTGGATGACACAAGCTCCTCCGAGGGCAGCACGGTGGACTGCCCGGACCCAGAGGA GATCCTGAGGAAGATCCCTGAGCTGGCGGAGGAGCTGGACGAGCCCGATGACTGTTTCCCAGAAGGCTGC ACTCGCCGCTGTCCCTGCTGCAAAGTGAACACCAGTAAGTTTCCTTGGGCCACGGGCTGGCAGGTGCGCA AAACCTGTTACCGCATTGTGGAGCACAGCTGGTTTGAGAGTTTCATTATCTTCATGATCCTGCTCAGCAG TGGAGCGCTGGCCTTTGAGGATAACTACCTGGAAGAAAAGCCCCGAGTGAAGTCTGTGCTGGAGTACACT GACAGAGTGTTCACCTTCATCTTTGTATTCGAGATGTTGCTCAAGTGGGTAGCTTATGGCTTCAAAAAAT ATTTCACCAATGCCTGGTGCTGGCTGGACTTCCTCATCGTGAATATCTCCCTCACAAGCCTCATAGCCAA GATCCTCGAGTATTCAGACGTGGCGTCCATCAAAGCCCTTCGGACTCTCCGTGCCCTCCGGCCGCTGCGG GCTCTGTCTCGATTCGAAGGCATGAGGGTAGTGGTGGATGCCTTGGTGGGCGCCATTCCCTCCATCATGA ACGTCCTCCTCGTCTGCCTCATCTTCTGGCTCATCTTCAGCATCATGGGTGTGAACCTCTTCGCCGGGAA ATTTTCGAGATGTGTCGACACCAGAAGCAACCCATTTTCCGTCGTGAATTCGACATTCGTGACTAACAAG TCTGACTGTTACAATCAAAACAATACTGGCCACTTCTTCTGGGTTAACGTCAAAGTCAACTTCGACAACG TTGCTATGGGCTACCTCGCGCTTCTCCAGGTGGCAACCTTCAAAGGCTGGATGGACATTATGTATGCAGC TGTCGATTCTCGAGATATCAACAGTCAGCCCAATTGGGAGGAGAGCCTGTACATGTACCTATACTTCGTC GTCTTCATCATTTTCGGTGGCTTCTTCACGCTGAATCTCTTTGTCGGGGTCATCATTGACAACTTCAATC AACAGAAAAAAAAGATAAGGGGCCAGGACATCTTCATGACAGAGGAGCAGAAGAAGTACTACAATGCCAT GAAGAAGCTGGGCTCCAAGAAACCCCAGAAGCCCATCCCACGGCCTTTGAATAAGTACCAGGGCTTCGTG TTTGACATTGTGACCAGGCAAGCATTTGACATCATCATCATGGCTCTCATCTGCCTCAACATGATCACCA TGATGGTGGAGACCGACAATCAGAGCGAGGAGAAGACGAAGGTCCTGGGCAGAATCAACCAGTTCTTCGT GGCCGTCTTCACGGGCGAGTGTGTGATGAAGATGTTCGCCCTTCGGCAGTATTACTTCACCAACGGCTGG AATGTGTTCGACTTCATTGTGGTGATTCTGTCCATTTCTAGTCTGTTGTTTTCTGCGATCCTTAGCTCAC TAGAAAGTTACTTCTCCCCCACGCTCTTACGCGTCATCCGTCTGGCCAGGATCGGCCGCATCCTCAGGCT GATTCGAGCAGCCAAGGGGATTCGCACGCTGCTCTTCGCCCTCATGATGTCCCTGCCCGCCCTCTTCAAC ATCGGCCTCCTCCTCTTCCTCGTCATGTTCATCTACTCCATCTTCGGCATGGCCAGCTTCGCTAATGTCA TAGATGAGGCTGGCATCGACGACATGTTCAACTTCAAGACCTTTGGCAACAGCATGCTGTGCCTTTTCCA GATCACCACGTCGGCTGGCTGGGATGGCCTCCTCAGCCCCATCCTCAACACAGGACCCCCCTACTGCGAC CCCAACCGGCCCAACAGCAATGGCTCCAAGGGGAATTGTGGAAGCCCAGCGGTGGGCATCCTCTTCTTCA CCACCTACATCATCATCTCCTTCCTCATCGTGGTCAACATGTACATTGCAGTGATTCTGGAGAACTTCAA TGTGGCCACAGAAGAGAGCACGGAGCCCCTGAGCGAGGACGACTTTGACATGTTCTATGAGACCTGGGAG AAGTTTGACCCGGAGGCCACCCAGTTCATTGCCTTTTCTGCCCTCTCAGACTTTGCAGACACACTCTCTG GCCCTCTTAGAATCCCAAAACCTAATCAGAATATATTAATCCAGATGGACCTGCCGTTGGTCCCCGGAGA TAAGATCCACTGTTTGGACATCCTCTTTGCCTTCACAAAGAATGTCTTGGGAGAATCTGGGGAGTTGGAT TCTCTGAAGACTAATATGGAAGAGAAGTTTATGGCAACTAATCTTTCCAAAGCATCCTATGAACCAATAG CAACCACCCTCCGGTGCAAGCAGGAAGACATCTCAGCCACCATTATTCAAAAGGCCTATCGGAACTACAT GTTGCAACGCTCCTTGATGCTCTCCAACCCCCTGCATGTGCCCAGGGCTGAGGAGGATGGCGTGTCACTC CCCAGGGAAGGCTATGTTACATTCATGGCAAATGACAACGGTGGGCTCCCAGACAAATCGGAAACTGCTT CTGCTACGTCTTTCCCACCATCCTATGACAGCGTCACCAGGGGCCTGAGTGACAGGGCCAACATTAGCAC ATCTAGCTCAATGCAAAATGAAGATGAAGTCACTGCTAAGGAAGGGAAGAGCCCTGGACCTCAG AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC TGGATTACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >MR231896 representing NM_001205321 Red=Cloning site Green=Tags(s) MEFPFGSVGTTNFRRFTPESLAEIEKQIAAHRAAKKGRPKQRGQKDKSEKPRPQLDLKACNQLPRFYGEL PAELVGEPLEDLDPFYSTHRTFIVLDKSRTISRFSATWALWLFSPFNLIRRTAIKVSVHSWFSIFITVTI LVNCVCMTRTDLPEKLEYAFTVVYTFEALIKILARGFCLNEFTYLRDPWNWLDFSVITLAYVGAAIDLRG ISGLRTFRVLRALKTVSVIPGLKVIVGALIHSVRKLADVTILTVFCLSVFALVGLQLFKGNLKNKCIKNG TDPHKADNLSSEMAGDIFIKPGTTDPLLCGNGSDAGHCPNDYVCRKTSDNPDFNYTSFDSFAWAFLSLFR LMTQDSWERLYQQTLRASGKMYMVFFVLVIFLGSFYLVNLILAVVTMAYEEQSQATIAEIEAKEKKFKEA LEVLQKEQEVLAALGIDTTSLYSHNGSPLAPKNANERRPRVKSRMSEGSTDDNRSLQSDPYNQRRMSFLG LSSGRRRASHSSVFHFRAPSQDVSFPDGILDDGVFHGDQESRRSSILLGRGAGQAGPLPRSPLPQSPNPG PRRGEEGQRGVPTGELATGAPEGPALDAAGQKNFLSADYLNEPFRAQRAMSVVSIMTSVIEELEESKLKC PPCLISLAQKYLIWECCPKWKKFKMVLFELVTDPFAELTITLCIVVNTVFMAMEHYPMTDAFDAMLQAGN IVFTVFFTMEMAFKIIAFDPYYYFQKKWNIFDCVIVTVSLLELSTSKKGSLSVLRTFRLLRVFKLAKSWP TLNMLIKIIGNSVGALGNLTFILAIIVFIFALVGKQLLSENYGCRRDGISVWNGERLRWHMCDFFHSFLV VFRILCGEWIENMWVCMEVSQDYICLTLFLTVMVLGNLVVLNLFIALLLNSFSADNLTAPEDDGEVNNLQ VALARIQVFGHRASRAITSYIRSHCRLRWPKVETQLGMKPPLTSCKAENHIATDAVNAAVGNLAKPALGG PKENHGDFITDPNVWVSVPIAEGESDLDELEEDVEHASQSSWQEESPKGQQELLQQVQKCEDHQAARSPP SGMSSEDLAPYLGERWQREESPRVPAEGVDDTSSSEGSTVDCPDPEEILRKIPELAEELDEPDDCFPEGC TRRCPCCKVNTSKFPWATGWQVRKTCYRIVEHSWFESFIIFMILLSSGALAFEDNYLEEKPRVKSVLEYT DRVFTFIFVFEMLLKWVAYGFKKYFTNAWCWLDFLIVNISLTSLIAKILEYSDVASIKALRTLRALRPLR ALSRFEGMRVVVDALVGAIPSIMNVLLVCLIFWLIFSIMGVNLFAGKFSRCVDTRSNPFSVVNSTFVTNK SDCYNQNNTGHFFWVNVKVNFDNVAMGYLALLQVATFKGWMDIMYAAVDSRDINSQPNWEESLYMYLYFV VFIIFGGFFTLNLFVGVIIDNFNQQKKKIRGQDIFMTEEQKKYYNAMKKLGSKKPQKPIPRPLNKYQGFV FDIVTRQAFDIIIMALICLNMITMMVETDNQSEEKTKVLGRINQFFVAVFTGECVMKMFALRQYYFTNGW NVFDFIVVILSISSLLFSAILSSLESYFSPTLLRVIRLARIGRILRLIRAAKGIRTLLFALMMSLPALFN IGLLLFLVMFIYSIFGMASFANVIDEAGIDDMFNFKTFGNSMLCLFQITTSAGWDGLLSPILNTGPPYCD PNRPNSNGSKGNCGSPAVGILFFTTYIIISFLIVVNMYIAVILENFNVATEESTEPLSEDDFDMFYETWE KFDPEATQFIAFSALSDFADTLSGPLRIPKPNQNILIQMDLPLVPGDKIHCLDILFAFTKNVLGESGELD SLKTNMEEKFMATNLSKASYEPIATTLRCKQEDISATIIQKAYRNYMLQRSLMLSNPLHVPRAEEDGVSL PREGYVTFMANDNGGLPDKSETASATSFPPSYDSVTRGLSDRANISTSSSMQNEDEVTAKEGKSPGPQ SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-RsrII      Cloning Scheme for this gene
ACCN	NM_001205321
ORF Size	5874 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001205321.1, NP_001192250.1
RefSeq Size	6416 bp
RefSeq ORF	5877 bp
Locus ID	20264
MW	221.1 kDa
Gene Summary	Tetrodotoxin-resistant channel that mediates the voltage-dependent sodium ion permeability of excitable membranes. Assuming opened or closed conformations in response to the voltage difference across the membrane, the protein forms a sodium-selective channel through which sodium ions may pass in accordance with their electrochemical gradient. Plays a role in neuropathic pain mechanisms.[UniProtKB/Swiss-Prot Function]

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