Cacna1d (NM_001302637) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	Myc-DDK
Synonyms	8430418G19Rik; C79217; Cach3; Cacn; Cacn4; Cacnl1a2; Cav1.3; Cchl; Cchl1; Cchl1a; Cchl1a2; D-LTC; D-LTCC
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MR231929 representing NM_001302637 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGATGATGATGATGATGATGAAAAAAATGCAACATCAACGGCAGCACCAAGAGGACCATGCGAACGAGG CAAACTATGCAAGAGGCACCAGACTCCCTATTTCTGGTGAAGGACCAACTTCTCAGCCCAACAGCTCCAA GCAAACTGTCCTATCTTGGCAAGCTGCAATCGATGCTGCTAGACAGGCCAAGGCCGCCCAGACCATGAGC ACCTCTGCACCCCCACCTGTAGGATCTCTCTCCCAAAGAAAACGTCAGCAATACGCCAAGAGCAAAAAAC AGGGTAACTCGTCTAACAGCCGACCTGCCCGTGCCCTTTTCTGTTTATCCCTCAATAACCCCATCCGAAG AGCCTGCATTAGTATAGTGGAATGGAAACCATTTGACATATTTATATTATTGGCTATTTTTGCCAATTGT GTGGCCTTAGCTATTTACATCCCATTCCCTGAAGATGATTCTAATTCAACAAATCATAACTTGGAAAAAG TAGAATATGCCTTCCTGATTATTTTTACAGTGGAGACATTTCTGAAGATTATAGCGTATGGACTGTTGCT GCATCCTAATGCTTACGTTAGGAATGGATGGAATTTACTGGATTTTGTTATAGTAATTGTAGGATTGTTT AGTGTAATTCTGGAACAATTAACCAAAGAAACAGAAGGCGGGAACCACTCTAGTGGCAAATCAGGAGGCT TTGATGTCAAAGCCCTCCGTGCCTTCCGAGTGTTAAGACCACTTCGACTTGTATCAGGAGTGCCCAGTTT ACAAGTTGTTCTGAACTCCATTATAAAAGCCATGGTCCCCCTCCTTCACATAGCCCTTTTGGTATTATTT GTAATTATCATCTATGCTATTATAGGATTGGAACTTTTTATTGGAAAAATGCACAAAACATGTTTTTTTG CTGACTCAGATATAGTAGCTGAAGAGGACCCAGCTCCGTGTGCGTTCTCAGGGAATGGACGCCAGTGCAC CGCCAATGGCACGGAATGTAGGAGTGGCTGGGTTGGCCCCAATGGAGGCATCACTAACTTTGATAACTTT GCCTTTGCCATGCTCACTGTGTTCCAGTGCATTACCATGGAGGGCTGGACAGATGTGCTCTACTGGGTTA ATGATGCGATAGGATGGGAATGGCCATGGGTGTATTTTGTTAGTCTGATCATCCTTGGCTCATTTTTCGT CCTTAACCTGGTTCTTGGTGTCCTTAGTGGAGAATTCTCCAAGGAAAGAGAGAAGGCTAAAGCTCGTGGA GATTTCCAGAAGCTTCGGGAAAAACAGCAGCTGGAAGAGGACCTCAAGGGCTACCTGGACTGGATCACCC AGGCAGAAGACATCGATCCCGAGAACGAGGAGGAGGGTGGCGAGGAAGGCAAACGAAACACTAGCATGCC CACCAGCGAGACTGAATCTGTCAACACAGAGAACGTAAGTGGTGAAGGCGAGACCCAGGGATGCTGTGGA ACTCTCTGGTGCTGGTGGAAAAGAAGAGGCGCGGCCAAGACCGGGCCCTCTGGGTGTCGGCGGTGGGGTC AGGCCATCTCGAAATCCAAACTCAGCCGTCGATGGCGTCGCTGGAACCGGTTCAATCGCAGGAGGTGCAG GGCTGCCGTGAAGTCTGTCACGTTTTACTGGCTGGTGATTGTCCTGGTGTTTCTCAACACTTTAACTATT TCCTCTGAGCATTACAATCAGCCAGACTGGCTGACACAGATTCAAGACATCGCAAACAAAGTCCTCTTGG CTCTGTTCACCTGTGAGATGCTGGTGAAGATGTACAGCCTGGGCCTCCAGGCTTACTTTGTCTCCCTCTT CAACCGCTTCGATTGCTTTGTGGTGTGTGGGGGCATCACCGAAACCATCCTGGTGGAACTGGAGCTCATG TCTCCCCTGGGGGTCTCCGTGTTCCGGTGTGTGCGCCTCCTGAGAATCTTCAAAGTGACCAGGCACTGGA CCTCCCTGAGCAACTTGGTGGCATCCTTGTTAAACTCCATGAAGTCCATCGCCTCTCTGCTGTTACTCCT CTTCCTCTTCATCATCATCTTCTCCTTGCTGGGGATGCAGTTGTTTGGTGGGAAGTTTAATTTTGACGAG ACTCAAACCAAGCGAAGCACCTTTGACAACTTTCCGCAAGCACTCCTGACGGTGTTCCAGATCCTGACAG GTGAAGACTGGAATGCGGTGATGTATGACGGCATCATGGCCTATGGAGGCCCTTCCTCTTCCGGGATGAT TGTCTGTATCTACTTCATCATCCTTTTCATCTGTGGTAACTATATCCTGCTGAATGTCTTCTTGGCCATC GCTGTGGACAATTTGGCTGATGCTGAAAGTCTGAACACTGCTCAGAAAGAGGAAGCTGAAGAAAAAGAAA GGAAAAAGATTGCCAGAAAAGAAAGCCTAGAAAACAAAAAGAACAACAAACCAGAAGTCAACCAGATAGC CAACAGTGACAACAAGGTTACAATTGATGATTATCAGGAAGATGCTGAAGACAAGGACCCTTACCCGCCC TGTGATGTGCCAGTAGGTGAAGAGGAAGAGGAGGAGGAAGAGGATGAGCCCGAAGTTCCTGCTGGTCCCC GCCCTCGCAGAATCTCAGAGTTGAACATGAAGGAGAAGATTGCGCCCATCCCAGAAGGAAGCGCCTTCTT CATTCTTAGCAAGACCAACCCGATACGTGTGGGTTGCCACAAGCTCATTAACCACCACATCTTCACCAAC CTTATCCTGGTCTTCATCATGCTGAGCAGCGCTGCCCTGGCTGCAGAGGACCCCATCCGTAGCCACTCAT TCCGCAACACGATACTGGGCTACTTTGACTATGCCTTCACAGCCATCTTTACGGTTGAAATCCTGTTAAA GATGACAACTTTTGGAGCCTTCTTGCACAAAGGGGCTTTCTGCAGGAACTACTTCAATTTGCTGGACATG CTGGTTGTTGGGGTGTCGCTGGTGTCCTTTGGGATTCAGTCCAGCGCCATCTCCGTTGTGAAGATTCTGA GGGTTTTGAGGGTCTTGCGGCCTCTCAGAGCAATCAACAGAGCAAAGGGACTTAAGCACGTGGTCCAGTG TGTCTTTGTGGCCATCCGAACCATTGGCAACATCATGATCGTCACGACCCTGCTCCAGTTCATGTTTGCA TGCATTGGGGTCCAGCTGTTCAAGGGGAAGTTCTACCGTTGCACAGATGAAGCCAAAAGTAACCCTGAGG AGTGCAGGGGGCTTTTCATTCTTTATAAGGACGGCGATGTTGACAGTCCTGTGGTCCGTGAGAGGATCTG GCAAAATAGTGATTTCAATTTTGACAATGTCCTTTCGGCTATGATGGCGCTGTTCACAGTCTCAACTTTT GAGGGCTGGCCCGCGTTGCTGTACAAAGCTATTGATTCAAACGGTGAGAACGTTGGTCCTGTCTACAACT ACCGTGTGGAGATCTCCATCTTCTTCATCATCTACATCATCATCGTTGCCTTCTTCATGATGAACATCTT TGTGGGCTTCGTCATCGTCACCTTCCAGGAACAGGGAGAAAAAGAGTATAAGAACTGTGAGCTGGACAAA AATCAGCGTCAGTGTGTTGAATATGCCTTGAAAGCACGCCCCTTAAGGAGATACATCCCCAAAAACCCAT ACCAGTACAAGTTCTGGTATGTGGTGAACTCCTCGCCTTTCGAATACATGATGTTCGTCCTCATCATGCT CAACACGCTCTGCCTGGCCATGCAGCACTATGAGCAGTCCAAGATGTTCAATGATGCCATGGACATTCTG AACATGGTCTTCACAGGGGTCTTCACTGTTGAGATGGTTTTGAAAGTCATTGCTTTCAAGCCCAAGGGGT ATTTTAGTGACGCCTGGAACACGTTTGACTCCCTCATCGTAATCGGCAGCATTATAGACGTGGCCCTCAG CGAAGCCGACCCATCTGAAAGTGAGACTATCCCTCTCCCAACTGCCACACCTGGGAACTCTGAAGAGAGC AATAGAATCTCCATCACCTTTTTCCGTCTTTTCCGAGTGATGCGGTTGGTGAAACTTCTCAGCAGGGGGG AAGGCATCCGGACACTGCTGTGGACCTTCATCAAGTCCTTTCAGGCGCTGCCGTATGTTGCCCTCCTCAT TGCCATGCTCTTCTTCATCTACGCTGTCATAGGCATGCAGATGTTTGGGAAAGTTGCCATGAGAGATAAC AACCAGATCAATAGGAACAACAACTTCCAGACCTTTCCTCAGGCAGTGCTCCTACTCTTCAGGTGTGCAA CAGGGGAAGCCTGGCAGGAGATCATGCTTGCCTGCCTCCCAGGAAAGCTGTGCGACCCAGACTCCGATTA CAACCCAGGAGAGGAGTACACTTGTGGGAGCAACTTTGCCATTGTCTACTTCATCAGCTTTTACATGCTC TGTGCATTCCTGATCATCAACCTCTTTGTGGCTGTCATCATGGACAATTTTGACTATCTGACTCGGGACT GGTCTATTCTGGGGCCTCACCACTTGGACGAATTCAAAAGAATATGGTCAGAATATGACCCTGAAGCAAA GGGAAGGATAAAACACCTTGATGTGGTCACTCTGCTCCGGCGGATCCAGCCTCCCCTGGGGTTTGGAAAA TTATGCCCACACCGAGTAGCATGTAAGAGATTGGTTGCCATGAACATGCCTCTCAACAGTGATGGGACAG TCATGTTCAATGCAACCCTGTTTGCTTTGGTCCGGACAGCTCTCAAGATCAAGACTGAAGGGAACCTGGA GCAAGCTAATGAAGAACTCCGAGCTGTGATCAAGAAAATCTGGAAGAAGACAAGCATGAAGCTGCTTGAC CAAGTTGTCCCTCCAGCTGGTGATGATGAGGTAACCGTGGGGAAGTTCTATGCCACTTTCCTGATACAGG ACTACTTTAGGAAATTCAAGAAACGGAAAGAGCAAGGACTGGTGGGGAAGTACCCTGCGAAGAACACCAC GATTGCCCTACAGGCGGGATTAAGGACCCTGCATGACATTGGGCCAGAAATCCGACGGGCTATATCCTGT GACTTGCAAGATGACGAGCCAGAAGACTCCAAACCAGAAGAAGAAGATGTATTCAAAAGAAATGGTGCCC TGCTTGGAAACCATGTCAATCATGTTAATAGTGATAGGAGAGATTCCCTTCAGCAGACCAATACCACCCA CCGTCCCCTGCATGTCCAAAGGCCTTCAATGCCACCTGCAAGTGATACTGAGAAACCGCTGTTTCCTCCA GCAGGAAATTCGGGGTGTCATAACCATCATAACCATAATTCCATAGGGAAGCAAGCTCCCACCTCAACAA ATGCCAATCTCAATAATGCCAATATGTCCAAAGCTGCCCACGGAAAGCCGCCCAGCATTGGGAACCTTGA GCATGTGTCTGAAAATGGGCATTACTCCTGCAAGCATGATCGAGAGCTTCAAAGAAGGTCCAGTATTAAA AGAACCCGCTATTACGAGACTTACATTAGGTCTGAGTCAGGAGATGAGCAATTTCCAACCATTTGCCGTG AAGATCCTGAGATACACGGCTACTTCCGAGACCCCCGCTGCTTGGGGGAACAGGAGTATTTCAGTAGCGA GGAGTGCTGTGAGGACGACAGCTCTCCCACCTGGAGCAGGCAAAACTACAACTACTACAACAGGTACCCA GGCAGCTCCATGGACTTTGAGAGGCCCCGAGGCTACCACCACCCCCAAGGCTTCTTAGAAGATGATGACT CTCCCACTGGCTATGATTCACGGAGATCTCCGAGGAGACGTCTTCTACCTCCTACCCCACCATCCCATCG GAGGTCCTCCTTCAACTTTGAGTGTCTGCGCAGGCAAAGCAGCCAAGACGATGTCCTTCCATCGCCTGCC CTGCCTCACCGCGCTGCCCTGCCCCTGCACCTGATGCAGCAGCAGATCATGGCCGTTGCAGGCCTAGATT CCAGTAAAGCCCAGAAGTACTCACCGAGCCACTCTACCCGGTCGTGGGCCACCCCTCCAGCAACCCCTCC ATACCGAGACTGGAGCCCATGCTACACCCCTTTGATCCAGGTGGACCGCTCAGAATCTATGGACCAGGTC AATGGCAGCCTGCCATCCCTACACCGAAGCTCCTGGTACACAGATGAACCCGACATCTCCTATAGGACTT TCACGCCAGCTAGTCTGACTGTCCCCAGCAGCTTCCGGAACAAGAACAGCGACAAGCAGAGGAGTGCAGA CAGCCTGGTGGAGGCAGTCCTGATATCCGAAGGCTTGGGACGCTACGCGAGGGACCCCAAATTTGTCTCA GCGACAAAACATGAGATTGCCGATGCCTGTGACCTAACCATAGACGAGATGGAAAGTGCTGCCAGCACCC TGCTCAATGGCAGTGTGTGTCCTCGAGCCAATGGGGACATGGGCCCCATCTCCCACCGCCAGGACTATGA GCTCCAGGACTTTGGTCCTGGCTACAGTGACGAGGAGCCAGACCCTGGGCGAGAGGAAGAGGACCTGGCA GATGAGATGATTTGCATTACCACCTTG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >MR231929 representing NM_001302637 Red=Cloning site Green=Tags(s) MMMMMMMKKMQHQRQHQEDHANEANYARGTRLPISGEGPTSQPNSSKQTVLSWQAAIDAARQAKAAQTMS TSAPPPVGSLSQRKRQQYAKSKKQGNSSNSRPARALFCLSLNNPIRRACISIVEWKPFDIFILLAIFANC VALAIYIPFPEDDSNSTNHNLEKVEYAFLIIFTVETFLKIIAYGLLLHPNAYVRNGWNLLDFVIVIVGLF SVILEQLTKETEGGNHSSGKSGGFDVKALRAFRVLRPLRLVSGVPSLQVVLNSIIKAMVPLLHIALLVLF VIIIYAIIGLELFIGKMHKTCFFADSDIVAEEDPAPCAFSGNGRQCTANGTECRSGWVGPNGGITNFDNF AFAMLTVFQCITMEGWTDVLYWVNDAIGWEWPWVYFVSLIILGSFFVLNLVLGVLSGEFSKEREKAKARG DFQKLREKQQLEEDLKGYLDWITQAEDIDPENEEEGGEEGKRNTSMPTSETESVNTENVSGEGETQGCCG TLWCWWKRRGAAKTGPSGCRRWGQAISKSKLSRRWRRWNRFNRRRCRAAVKSVTFYWLVIVLVFLNTLTI SSEHYNQPDWLTQIQDIANKVLLALFTCEMLVKMYSLGLQAYFVSLFNRFDCFVVCGGITETILVELELM SPLGVSVFRCVRLLRIFKVTRHWTSLSNLVASLLNSMKSIASLLLLLFLFIIIFSLLGMQLFGGKFNFDE TQTKRSTFDNFPQALLTVFQILTGEDWNAVMYDGIMAYGGPSSSGMIVCIYFIILFICGNYILLNVFLAI AVDNLADAESLNTAQKEEAEEKERKKIARKESLENKKNNKPEVNQIANSDNKVTIDDYQEDAEDKDPYPP CDVPVGEEEEEEEEDEPEVPAGPRPRRISELNMKEKIAPIPEGSAFFILSKTNPIRVGCHKLINHHIFTN LILVFIMLSSAALAAEDPIRSHSFRNTILGYFDYAFTAIFTVEILLKMTTFGAFLHKGAFCRNYFNLLDM LVVGVSLVSFGIQSSAISVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIRTIGNIMIVTTLLQFMFA CIGVQLFKGKFYRCTDEAKSNPEECRGLFILYKDGDVDSPVVRERIWQNSDFNFDNVLSAMMALFTVSTF EGWPALLYKAIDSNGENVGPVYNYRVEISIFFIIYIIIVAFFMMNIFVGFVIVTFQEQGEKEYKNCELDK NQRQCVEYALKARPLRRYIPKNPYQYKFWYVVNSSPFEYMMFVLIMLNTLCLAMQHYEQSKMFNDAMDIL NMVFTGVFTVEMVLKVIAFKPKGYFSDAWNTFDSLIVIGSIIDVALSEADPSESETIPLPTATPGNSEES NRISITFFRLFRVMRLVKLLSRGEGIRTLLWTFIKSFQALPYVALLIAMLFFIYAVIGMQMFGKVAMRDN NQINRNNNFQTFPQAVLLLFRCATGEAWQEIMLACLPGKLCDPDSDYNPGEEYTCGSNFAIVYFISFYML CAFLIINLFVAVIMDNFDYLTRDWSILGPHHLDEFKRIWSEYDPEAKGRIKHLDVVTLLRRIQPPLGFGK LCPHRVACKRLVAMNMPLNSDGTVMFNATLFALVRTALKIKTEGNLEQANEELRAVIKKIWKKTSMKLLD QVVPPAGDDEVTVGKFYATFLIQDYFRKFKKRKEQGLVGKYPAKNTTIALQAGLRTLHDIGPEIRRAISC DLQDDEPEDSKPEEEDVFKRNGALLGNHVNHVNSDRRDSLQQTNTTHRPLHVQRPSMPPASDTEKPLFPP AGNSGCHNHHNHNSIGKQAPTSTNANLNNANMSKAAHGKPPSIGNLEHVSENGHYSCKHDRELQRRSSIK RTRYYETYIRSESGDEQFPTICREDPEIHGYFRDPRCLGEQEYFSSEECCEDDSSPTWSRQNYNYYNRYP GSSMDFERPRGYHHPQGFLEDDDSPTGYDSRRSPRRRLLPPTPPSHRRSSFNFECLRRQSSQDDVLPSPA LPHRAALPLHLMQQQIMAVAGLDSSKAQKYSPSHSTRSWATPPATPPYRDWSPCYTPLIQVDRSESMDQV NGSLPSLHRSSWYTDEPDISYRTFTPASLTVPSSFRNKNSDKQRSADSLVEAVLISEGLGRYARDPKFVS ATKHEIADACDLTIDEMESAASTLLNGSVCPRANGDMGPISHRQDYELQDFGPGYSDEEPDPGREEEDLA DEMICITTL TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001302637
ORF Size	6537 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001302637.1, NP_001289566.1
RefSeq Size	9180 bp
RefSeq ORF	6540 bp
Locus ID	12289
UniProt ID	Q99246
MW	247.5 kDa
Gene Summary	This gene encodes a pore-forming subunit of the L-type, voltage-activated calcium channel family. These channels have been found to play a role in heart and smooth muscle contraction and in the transmission of auditory information. Homozygous knockout mice for this gene exhibit deafness and heart defects. These channels have also been linked to mitochondrial oxidative stress in a mouse model of Parkinson's disease. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Nov 2014]

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