CD70 (NM_001252) Human Tagged ORF Clone
CAT#: RC200410
CD70 (Myc-DDK-tagged)-Human CD70 molecule (CD70)
ORF Plasmid: tGFP
"NM_001252" in other vectors (6)
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CNY 2,400.00
CNY 3,705.00
Cited in 3 publications. |
CNY 300.00
CNY 1,999.00
CNY 2,700.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | CD27-L; CD27L; CD27LG; LPFS3; TNFSF7; TNLG8A |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC200410 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCGGAGGAGGGTTCGGGCTGCTCGGTGCGGCGCAGGCCCTATGGGTGCGTCCTGCGGGCTGCTTTGG TCCCATTGGTCGCGGGCTTGGTGATCTGCCTCGTGGTGTGCATCCAGCGCTTCGCACAGGCTCAGCAGCA GCTGCCGCTCGAGTCACTTGGGTGGGACGTAGCTGAGCTGCAGCTGAATCACACAGGACCTCAGCAGGAC CCCAGGCTATACTGGCAGGGGGGCCCAGCACTGGGCCGCTCCTTCCTGCATGGACCAGAGCTGGACAAGG GGCAGCTACGTATCCATCGTGATGGCATCTACATGGTACACATCCAGGTGACGCTGGCCATCTGTTCCTC CACGACGGCCTCCAGGCACCACCCCACCACCCTGGCCGTGGGAATCTGCTCTCCCGCCTCCCGTAGCATC AGCCTGCTGCGTCTCAGCTTCCACCAAGGTTGTACCATTGCCTCCCAGCGCCTGACGCCCCTGGCCCGAG GGGACACACTCTGCACCAACCTCACTGGGACACTTTTGCCTTCCCGAAACACTGATGAGACCTTCTTTGG AGTGCAGTGGGTGCGCCCC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC200410 protein sequence
Red=Cloning site Green=Tags(s) MPEEGSGCSVRRRPYGCVLRAALVPLVAGLVICLVVCIQRFAQAQQQLPLESLGWDVAELQLNHTGPQQD PRLYWQGGPALGRSFLHGPELDKGQLRIHRDGIYMVHIQVTLAICSSTTASRHHPTTLAVGICSPASRSI SLLRLSFHQGCTIASQRLTPLARGDTLCTNLTGTLLPSRNTDETFFGVQWVRP TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
Sequencher program is needed, download here. |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_001252 |
ORF Size | 579 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001252.5 |
RefSeq Size | 913 bp |
RefSeq ORF | 582 bp |
Locus ID | 970 |
UniProt ID | P32970 |
Protein Families | ES Cell Differentiation/IPS, Transmembrane |
Protein Pathways | Cytokine-cytokine receptor interaction |
MW | 21.1 kDa |
Gene Summary | The protein encoded by this gene is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family. This cytokine is a ligand for TNFRSF27/CD27. It is a surface antigen on activated, but not on resting, T and B lymphocytes. It induces proliferation of costimulated T cells, enhances the generation of cytolytic T cells, and contributes to T cell activation. This cytokine is also reported to play a role in regulating B-cell activation, cytotoxic function of natural killer cells, and immunoglobulin sythesis. [provided by RefSeq, Jul 2008] |
Citations (3)
The use of this cDNA Clones has been cited in the following citations: |
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Single amino acid substitution in LC-CDR1 induces Russell body phenotype that attenuates cellular protein synthesis through eIF2α phosphorylation and thereby downregulates IgG secretion despite operational secretory pathway traffic
,Hasegawa, H;Hsu, A;Tinberg, CE;Siegler, KE;Nazarian, AA;Tsai, MM;,
MAbs
,PubMed ID 28379093
[CD70]
|
Combined immunodeficiency and Epstein-Barr virus-induced B cell malignancy in humans with inherited CD70 deficiency
,Abolhassani, H;Edwards, ES;Ikinciogullari, A;Jing, H;Borte, S;Buggert, M;Du, L;Matsuda-Lennikov, M;Romano, R;Caridha, R;Bade, S;Zhang, Y;Frederiksen, J;Fang, M;Bal, SK;Haskologlu, S;Dogu, F;Tacyildiz, N;Matthews, HF;McElwee, JJ;Gostick, E;Price, DA;Palendira, U;Aghamohammadi, A;Boisson, B;Rezaei, N;Karlsson, AC;Lenardo, MJ;Casanova, JL;Hammarström, L;Tangye, SG;Su, HC;Pan-Hammarström, Q;,
J. Exp. Med
,PubMed ID 28011864
[CD70]
|
Characterization of the human T cell response to in vitro CD27 costimulation with varlilumab
,Ramakrishna, V;Sundarapandiyan, K;Zhao, B;,
Journal for ImmunoTherapy of Cancer
[CD70]
|
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC200410L1 | Lenti ORF clone of Human CD70 molecule (CD70), Myc-DDK-tagged |
CNY 4,800.00 |
|
RC200410L2 | Lenti ORF clone of Human CD70 molecule (CD70), mGFP tagged |
CNY 5,890.00 |
|
RC200410L3 | Lenti ORF clone of Human CD70 molecule (CD70), Myc-DDK-tagged |
CNY 4,800.00 |
|
RC200410L4 | Lenti ORF clone of Human CD70 molecule (CD70), mGFP tagged |
CNY 4,800.00 |
|
RG200410 | CD70 (tGFP-tagged) - Human CD70 molecule (CD70) |
CNY 4,000.00 |
|
SC122587 | CD70 (untagged)-Human CD70 molecule (CD70) |
CNY 2,400.00 |