SLC35B4 (NM_032826) Human Tagged ORF Clone

CAT#: RC203263

  • TrueORF®

SLC35B4 (Myc-DDK-tagged)-Human solute carrier family 35, member B4 (SLC35B4)

ORF Plasmid: DDK tGFP



  "NM_032826" in other vectors (4)

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CNY 2,400.00

CNY 3,990.00


货期*
现货

规格
    • 10 ug

Cited in 1 publication.

Product images

经常一起买 (4)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

CNY 300.00

Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Synonyms YEA; YEA4
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC203263 representing NM_032826
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCGCCCGGCCTTGGCGGTGGGCCTGGTGTTCGCAGGCTGCTGCAGTAACGTGATCTTCCTAGAGCTCC
TGGCCCGGAAGCATCCAGGATGTGGGAACATTGTGACATTTGCACAATTTTTATTTATTGCTGTGGAAGG
CTTCCTCTTTGAAGCTGATTTGGGAAGGAAGCCACCAGCTATCCCAATAAGGTACTATGCCATAATGGTG
ACCATGTTCTTCACCGTGAGCGTGGTGAACAACTATGCCCTGAATCTCAACATTGCCATGCCCCTGCATA
TGATATTTAGATCCGGTTCTCTAATTGCCAACATGATTCTAGGAATTATCATTTTGAAGAAAAGATACAG
TATATTCAAATATACCTCCATTGCCCTGGTGTCTGTGGGGATATTTATTTGCACTTTTATGTCAGCAAAG
CAGGTGACTTCCCAGTCCAGCTTGAGTGAGAATGATGGATTCCAGGCATTTGTGTGGTGGTTACTAGGTA
TTGGGGCATTGACTTTTGCTCTTCTGATGTCAGCAAGGATGGGGATATTCCAAGAGACTCTCTACAAACG
ATTTGGGAAACACTCCAAGGAGGCTTTGTTTTATAATCACGCCCTTCCACTTCCGGGTTTCGTCTTCTTG
GCTTCTGATATTTATGACCATGCAGTTCTATTCAATAAGTCTGAGTTATATGAAATTCCCGTCATCGGAG
TGACCCTGCCCATCATGTGGTTCTACCTCCTCATGAACATCATCACTCAGTACGTGTGCATCCGGGGTGT
GTTTATCCTCACCACGGAATGCGCCTCCCTCACCGTCACGCTCGTCGTGACCCTACGCAAATTTGTGAGC
CTCATCTTTTCCATCTTGTACTTCCAGAACCCCTTCACCCTGTGGCACTGGCTGGGCACCTTGTTTGTCT
TCATTGGGACCTTAATGTACACAGAGGTGTGGAACAACCTAGGGACCACAAAAAGTGAGCCTCAGAAGGA
CAGCAAGAAGAAC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC203263 representing NM_032826
Red=Cloning site Green=Tags(s)

MRPALAVGLVFAGCCSNVIFLELLARKHPGCGNIVTFAQFLFIAVEGFLFEADLGRKPPAIPIRYYAIMV
TMFFTVSVVNNYALNLNIAMPLHMIFRSGSLIANMILGIIILKKRYSIFKYTSIALVSVGIFICTFMSAK
QVTSQSSLSENDGFQAFVWWLLGIGALTFALLMSARMGIFQETLYKRFGKHSKEALFYNHALPLPGFVFL
ASDIYDHAVLFNKSELYEIPVIGVTLPIMWFYLLMNIITQYVCIRGVFILTTECASLTVTLVVTLRKFVS
LIFSILYFQNPFTLWHWLGTLFVFIGTLMYTEVWNNLGTTKSEPQKDSKKN

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_032826
ORF Size 993 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_032826.5
RefSeq Size 6794 bp
RefSeq ORF 996 bp
Locus ID 84912
UniProt ID Q969S0
Protein Families Transmembrane
MW 37.2 kDa
Gene Summary Glycosyltransferases, such as SLC35B4, transport nucleotide sugars from the cytoplasm where they are synthesized, to the Golgi apparatus where they are utilized in the synthesis of glycoproteins, glycolipids, and proteoglycans (Ashikov et al., 2005 [PubMed 15911612]).[supplied by OMIM, Mar 2008]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Citations (1)

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