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SLD5 (GINS4) (NM_032336) Human Tagged ORF Clone
CAT＃: RC203336
GINS4 (Myc-DDK-tagged)-Human GINS complex subunit 4 (Sld5 homolog) (GINS4)
ORF Plasmid: tGFP
"NM_032336" in other vectors (6)
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CNY 2,400.00
CNY 3,705.00
| Cited in 1 publication. |
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | SLD5 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC203336 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACCGAAGAAGTGGATTTCCTGGGACAGGACTCTGATGGGGGTAGTGAGGAAGTGGTCCTAACTCCTG CAGAGCTCATTGAAAGATTGGAGCAGGCCTGGATGAATGAAAAGTTTGCCCCTGAGCTGCTGGAGAGCAA GCCTGAGATTGTAGAATGTGTCATGGAACAGCTGGAGCACATGGAAGAAAATCTCAGGAGAGCCAAAAGG GAGGACCTGAAGGTCAGCATCCACCAAATGGAGATGGAGAGGATCCGCTACGTCCTCAGCAGCTACTTGC GGTGTCGCCTCATGAAGATAGAGAAGTTTTTCCCTCATGTCCTTGAGAAGGAAAAAACACGTCCTGAGGG GGAGCCTTCCAGCCTCTCGCCGGAAGAGTTGGCCTTTGCCAGAGAGTTCATGGCGAACACAGAGTCCTAT CTGAAAAATGTCGCCTTGAAGCACATGCCCCCTAACTTACAGAAGGTGGACCTCTTTCGGGCAGTTCCCA AACCAGATCTAGATTCTTACGTGTTTCTGAGAGTGAGAGAACGACAAGAAAACATACTGGTAGAACCAGA CACAGATGAGCAGAGGGACTACGTGATTGACCTGGAGAAGGGCTCACAGCACTTGATCCGATACAAAACC ATTGCACCTCTGGTTGCATCTGGAGCTGTCCAGCTAATT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC203336 protein sequence
Red=Cloning site Green=Tags(s) MTEEVDFLGQDSDGGSEEVVLTPAELIERLEQAWMNEKFAPELLESKPEIVECVMEQLEHMEENLRRAKR EDLKVSIHQMEMERIRYVLSSYLRCRLMKIEKFFPHVLEKEKTRPEGEPSSLSPEELAFAREFMANTESY LKNVALKHMPPNLQKVDLFRAVPKPDLDSYVFLRVRERQENILVEPDTDEQRDYVIDLEKGSQHLIRYKT IAPLVASGAVQLI TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_032336 |
| ORF Size | 669 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_032336.3 |
| RefSeq Size | 3841 bp |
| RefSeq ORF | 672 bp |
| Locus ID | 84296 |
| UniProt ID | Q9BRT9 |
| MW | 26 kDa |
| Gene Summary | The yeast heterotetrameric GINS complex is made up of Sld5, Psf1 (GINS1; MIM 610608), Psf2 (GINS2; MIM 610609), and Psf3 (GINS3; MIM 610610). The formation of the GINS complex is essential for the initiation of DNA replication in yeast and Xenopus egg extracts (Ueno et al., 2005 [PubMed 16287864]). See GINS1 for additional information about the GINS complex.[supplied by OMIM, Mar 2008] |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
ATR kinase inhibition induces unscheduled origin firing through a Cdc7-dependent association between GINS and And-1
,Moiseeva, T;Hood, B;Schamus, S;O'Connor, MJ;Conrads, TP;Bakkenist, CJ;,
Nat Commun
,PubMed ID 29123096
[SLD5]
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Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC203336L1 | Lenti ORF clone of Human GINS complex subunit 4 (Sld5 homolog) (GINS4), Myc-DDK-tagged |
CNY 4,800.00 |
|
| RC203336L2 | Lenti ORF clone of Human GINS complex subunit 4 (Sld5 homolog) (GINS4), mGFP tagged |
CNY 5,890.00 |
|
| RC203336L3 | Lenti ORF clone of Human GINS complex subunit 4 (Sld5 homolog) (GINS4), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC203336L4 | Lenti ORF clone of Human GINS complex subunit 4 (Sld5 homolog) (GINS4), mGFP tagged |
CNY 5,890.00 |
|
| RG203336 | GINS4 (tGFP-tagged) - Human GINS complex subunit 4 (Sld5 homolog) (GINS4) |
CNY 4,000.00 |
|
| SC123022 | GINS4 (untagged)-Human GINS complex subunit 4 (Sld5 homolog) (GINS4) |
CNY 3,990.00 |
