PHLPP (PHLPP1) (NM_194449) Human Tagged ORF Clone

CAT＃: RC203930

PHLPP1 (Myc-DDK-tagged)-Human PH domain and leucine rich repeat protein phosphatase 1 (PHLPP1)

ORF Plasmid: DDK tGFP

Datasheet
SDS
Plasmid Map

  "NM_194449" in other vectors (6)

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CNY 8,392.00

货期*
现货
规格
    • 10 ug
Cited in 1 publication.

Product images

经常一起买 (5)
PS100001
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00

添加到购物车
CC100003
DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00

暂停销售
PRIMER
Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

添加到购物车
TA592569S
DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

CNY 300.00

添加到购物车

【特别福利】购一抗正装产品，免费领10ul抗体试用装

TA319850
Rabbit Polyclonal PHLPP1 Antibody
    • 100 ug

CNY 5,808.00

添加到购物车

Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Synonyms PHLPP; PLEKHE1; PPM3A; SCOP
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC203930 representing NM_194449
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGACTCGGAGATTGGCTGCCTCATCCGCTTCTATGCAGGAAAACCTCACAGCACGGGTAGCTCTGAAC
GGATTCAGCTCTCAGGAATGTATAATGTCCGTAAAGGCAAGATGCAGTTGCCAGTGAACCGATGGACAAG
ACGCCAAGTCATCCTATGTGGGACCTGCCTGATAGTATCATCTGTGAAAGACAGCTTGACCGGAAAGATG
CATGTTCTGCCACTAATTGGTGGAAAAGTAGAAGAAGTGAAAAAGCACCAACACTGTTTAGCATTTAGCT
CCTCTGGACCCCAAAGCCAGACTTACTACATTTGCTTTGATACTTTCACAGAATACTTAAGGTGGCTGCG
ACAAGTCTCCAAGGTTGCATCCCAGCGCATTAGCTCAGTGGACCTCTCGTGTTGTAGCCTGGAACATCTG
CCTGCCAACCTCTTCTACAGCCAAGACCTCACTCATCTCAATTTAAAACAAAACTTCCTAAGGCAGAACC
CTAGCCTTCCAGCTGCCAGGGGGCTTAATGAACTGCAAAGGTTCACCAAGTTGAAGAGTCTTAACCTTTC
CAATAATCATTTAGGGGACTTCCCTCTGGCAGTCTGCAGTATTCCAACCCTGGCAGAGCTGAACGTGTCC
TGCAATGCCCTGCGATCAGTCCCGGCAGCCGTTGGAGTGATGCACAACTTACAGACATTTTTGTTGGATG
GAAACTTTCTCCAATCCCTTCCTGCTGAGTTGGAGAACATGAAGCAGCTTAGTTATCTGGGTCTTTCTTT
CAATGAATTTACTGACATTCCCGAAGTATTGGAGAAATTGACTGCTGTGGATAAACTTTGTATGTCTGGA
AACTGTGTGGAGACCCTTAGGCTACAGGCTTTAAGAAAAATGCCTCACATTAAACATGTGGATCTAAGGT
TGAACGTAATTAGGAAGCTGATAGCAGATGAAGTGGACTTTCTACAGCATGTTACTCAGCTTGACCTACG
AGACAATAAGCTTGGTGATCTAGATGCTATGATTTTCAACAACATTGAAGTTTTACACTGTGAAAGGAAT
CAACTGGTCACATTAGACATCTGTGGCTATTTCCTAAAAGCGCTCTATGCCTCTTCTAATGAACTTGTTC
AACTTGATGTTTACCCAGTTCCAAATTATCTGTCCTACATGGATGTTTCAAGGAACCGCTTAGAAAATGT
GCCTGAGTGGGTATGTGAAAGCCGAAAGCTAGAAGTTTTGGATATTGGCCATAATCAAATATGTGAACTT
CCTGCCCGCTTATTTTGTAATAGCAGTCTCCGGAAACTACTGGCAGGACACAACCAGTTGGCAAGGCTGC
CTGAAAGGCTAGAAAGAACCTCGGTGGAGGTCTTGGATGTGCAACACAACCAGCTCCTTGAGCTCCCACC
TAACCTTCTGATGAAGGCTGACAGCCTGAGATTCCTGAACGCCTCTGCGAACAAACTGGAAAGCCTTCCT
CCAGCCACGCTTTCCGAAGAGACAAACAGTATCTTACAAGAGTTGTATTTGACAAATAACAGCCTCACAG
ACAAATGTGTGCCCTTGTTAACGGGACACCCCCATTTGAAGATCCTTCACATGGCCTATAACCGACTTCA
GAGTTTTCCAGCAAGTAAAATGGCGAAACTGGAGGAACTTGAAGAAATTGATCTCAGTGGGAATAAGCTG
AAAGCCATCCCAACAACGATCATGAATTGCAGGCGCATGCACACCGTGATTGCTCACTCCAACTGCATCG
AGGTCTTTCCCGAAGTTATGCAGCTCCCAGAGATCAAGTGTGTGGACCTGAGCTGTAATGAGCTAAGTGA
AGTCACATTACCAGAAAACCTGCCTCCCAAACTGCAGGAGCTAGACCTGACTGGAAACCCGCGCCTTGTC
CTTGATCACAAAACCCTGGAACTACTGAATAATATCCGCTGTTTCAAGATTGATCAGCCTTCTACAGGAG
ACGCTTCCGGAGCCCCAGCTGTATGGAGTCATGGTTACACTGAAGCTTCGGGGGTAAAAAACAAGTTGTG
TGTCGCAGCCCTGTCGGTGAATAACTTCTGTGACAACCGCGAAGCCCTGTATGGTGTGTTTGACGGAGAC
CGGAATGTGGAGGTGCCCTACCTTCTCCAGTGCACTATGAGTGACATTTTGGCTGAAGAGCTGCAAAAAA
CAAAAAACGAAGAAGAATACATGGTCAATACATTCATTGTCATGCAAAGGAAACTTGGAACTGCTGGGCA
GAAGCTTGGTGGTGCCGCTGTCCTTTGTCATATCAAGCATGACCCTGTGGATCCAGGAGGATCCTTCACC
TTGACCTCTGCTAATGTGGGCAAGTGCCAAACAGTTCTCTGTCGAAATGGAAAGCCGCTGCCTCTGTCCA
GATCTTACATCATGAGCTGTGAAGAAGAGCTGAAGAGGATTAAACAGCACAAGGCCATTATCACTGAGGA
TGGCAAGGTGAACGGAGTGACTGAGTCCACGCGCATCCTGGGCTACACCTTCCTCCATCCCAGTGTGGTG
CCTCGCCCCCACGTGCAGTCCGTGCTCCTGACTCCCCAGGATGAGTTCTTCATCCTAGGCAGTAAGGGGT
TGTGGGACAGCCTGTCCGTCGAGGAGGCCGTGGAAGCCGTGCGCAACGTGCCCGATGCCCTGGCTGCTGC
CAAGAAGCTGTGTACCCTGGCCCAGAGCTACGGCTGCCACGACAGCATCAGCGCTGTGGTGGTGCAGCTC
AGTGTCACTGAGGACAGCTTCTGCTGCTGCGAGCTCAGCGCCGGTGGGGCTGTGCCACCACCCAGTCCTG
GCATCTTTCCTCCCTCAGTGAACATGGTGATCAAGGATCGGCCCTCAGATGGGCTGGGCGTGCCGTCCTC
CAGCAGCGGCATGGCTTCCGAGATTAGCAGTGAGCTCTCCACTTCTGAGATGAGCAGCGAGGTGGGGTCA
ACAGCCTCCGATGAGCCCCCGCCCGGAGCCCTAAGCGAGAACAGCCCTGCCTACCCCAGTGAGCAGCGCT
GCATGCTCCACCCCATCTGTCTGTCCAACTCCTTCCAGCGCCAGCTATCCAGCGCCACGTTCTCTAGCGC
CTTCTCCGACAACGGCCTTGACAGTGACGATGAGGAGCCCATCGAGGGCGTCTTCACCAACGGCAGCCGG
GTGGAGGTGGAGGTGGACATCCACTGCAGCCGGGCCAAGGAGAAGGAGAAACAGCAGCACCTGCTTCAGG
TGCCAGCAGAGGCCAGTGATGAGGGCATTGTCATCAGCGCCAACGAGGATGAGCCAGGTCTGCCCAGGAA
GGCAGACTTCTCTGCCGTTGGGACCATTGGGCGCCGGAGGGCCAATGGCTCTGTTGCGCCCCAGGAAAGG
AGCCACAATGTGATAGAGGTGGCTACAGACGCACCTCTTCGAAAGCCTGGAGGCTATTTTGCTGCCCCGG
CTCAGCCGGATCCTGATGATCAGTTTATCATACCCCCGGAGCTGGAAGAGGAGGTCAAAGAAATCATGAA
GCATCACCAGGAGCAACAGCAGCAGCAGCAGCCGCCACCACCCCCTCAGCTCCAGCCGCAGCTGCCGCGG
CACTACCAGCTGGACCAGCTGCCAGATTATTACGACACGCCACTA

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
>RC203930 representing NM_194449
Red=Cloning site Green=Tags(s)

MDSEIGCLIRFYAGKPHSTGSSERIQLSGMYNVRKGKMQLPVNRWTRRQVILCGTCLIVSSVKDSLTGKM
HVLPLIGGKVEEVKKHQHCLAFSSSGPQSQTYYICFDTFTEYLRWLRQVSKVASQRISSVDLSCCSLEHL
PANLFYSQDLTHLNLKQNFLRQNPSLPAARGLNELQRFTKLKSLNLSNNHLGDFPLAVCSIPTLAELNVS
CNALRSVPAAVGVMHNLQTFLLDGNFLQSLPAELENMKQLSYLGLSFNEFTDIPEVLEKLTAVDKLCMSG
NCVETLRLQALRKMPHIKHVDLRLNVIRKLIADEVDFLQHVTQLDLRDNKLGDLDAMIFNNIEVLHCERN
QLVTLDICGYFLKALYASSNELVQLDVYPVPNYLSYMDVSRNRLENVPEWVCESRKLEVLDIGHNQICEL
PARLFCNSSLRKLLAGHNQLARLPERLERTSVEVLDVQHNQLLELPPNLLMKADSLRFLNASANKLESLP
PATLSEETNSILQELYLTNNSLTDKCVPLLTGHPHLKILHMAYNRLQSFPASKMAKLEELEEIDLSGNKL
KAIPTTIMNCRRMHTVIAHSNCIEVFPEVMQLPEIKCVDLSCNELSEVTLPENLPPKLQELDLTGNPRLV
LDHKTLELLNNIRCFKIDQPSTGDASGAPAVWSHGYTEASGVKNKLCVAALSVNNFCDNREALYGVFDGD
RNVEVPYLLQCTMSDILAEELQKTKNEEEYMVNTFIVMQRKLGTAGQKLGGAAVLCHIKHDPVDPGGSFT
LTSANVGKCQTVLCRNGKPLPLSRSYIMSCEEELKRIKQHKAIITEDGKVNGVTESTRILGYTFLHPSVV
PRPHVQSVLLTPQDEFFILGSKGLWDSLSVEEAVEAVRNVPDALAAAKKLCTLAQSYGCHDSISAVVVQL
SVTEDSFCCCELSAGGAVPPPSPGIFPPSVNMVIKDRPSDGLGVPSSSSGMASEISSELSTSEMSSEVGS
TASDEPPPGALSENSPAYPSEQRCMLHPICLSNSFQRQLSSATFSSAFSDNGLDSDDEEPIEGVFTNGSR
VEVEVDIHCSRAKEKEKQQHLLQVPAEASDEGIVISANEDEPGLPRKADFSAVGTIGRRRANGSVAPQER
SHNVIEVATDAPLRKPGGYFAAPAQPDPDDQFIIPPELEEEVKEIMKHHQEQQQQQQPPPPPQLQPQLPR
HYQLDQLPDYYDTPL

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_194449
ORF Size 3615 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_194449.1, NP_919431.1
RefSeq Size 4798 bp
RefSeq ORF 5154 bp
Locus ID 23239
UniProt ID O60346
MW 184.5 kDa
Gene Summary This gene encodes a member of the serine/threonine phosphatase family. The encoded protein promotes apoptosis by dephosphorylating and inactivating the serine/threonine kinase Akt, and functions as a tumor suppressor in multiple types of cancer. Increased expression of this gene may also play a role in obesity and type 2 diabetes by interfering with Akt-mediated insulin signaling. [provided by RefSeq, Dec 2011]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Citations (1)

The use of this cDNA Clones has been cited in the following citations:
PHLPP1 splice variants differentially regulate AKT and PKC? signaling in hippocampal neurons: characterization of PHLPP proteins in the adult hippocampus ,null, Journal of neurochemistry ,PubMed ID 20819118 [PHLPP]

Other Versions

SKU Description Size Price
RC203930L1 Lenti ORF clone of Human PH domain and leucine rich repeat protein phosphatase 1 (PHLPP1), Myc-DDK-tagged
    • 10 ug

CNY 10,792.00

RC203930L2 Lenti ORF clone of Human PH domain and leucine rich repeat protein phosphatase 1 (PHLPP1), mGFP tagged
    • 10 ug

CNY 13,970.00

RC203930L3 Lenti ORF clone of Human PH domain and leucine rich repeat protein phosphatase 1 (PHLPP1), Myc-DDK-tagged
    • 10 ug

CNY 10,792.00

RC203930L4 Lenti ORF clone of Human PH domain and leucine rich repeat protein phosphatase 1 (PHLPP1), mGFP tagged
    • 10 ug

CNY 10,792.00

RG203930 PHLPP1 (tGFP-tagged) - Human PH domain and leucine rich repeat protein phosphatase 1 (PHLPP1)
    • 10 ug

CNY 9,992.00

SC108817 PHLPP1 (untagged)-Human PH domain and leucine rich repeat protein phosphatase 1 (PHLPP1)
    • 10 ug

CNY 8,400.00

You may also need
plasmid icon Empty Vector
protein iconDDK/Flag Antibody

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CRISPR
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CNY 10,792.00

RG203930

PHLPP1 (tGFP-tagged) - Human PH domain and leucine rich repeat protein phosphatase 1 (PHLPP1)

    • 10 ug

CNY 9,992.00

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