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NASP (NM_002482) Human Tagged ORF Clone
CAT＃: RC204052
NASP (Myc-DDK-tagged)-Human nuclear autoantigenic sperm protein (histone-binding) (NASP), transcript variant 2
ORF Plasmid: tGFP
"NM_002482" in other vectors (7)
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CNY 5,776.00
CNY 5,800.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | FLB7527; HMDRA1; PRO1999 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC204052 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCATGGAGTCCACAGCCACTGCCGCCGTCGCCGCGGAGCTGGTTTCTGCCGACAAAATTGAAGATG TTCCTGCTCCTTCTACATCTGCAGATAAAGTGGAGAGTCTGGATGTGGATAGTGAAGCTAAGAAACTATT GGGTTTAGGACAGAAACATCTGGTGATGGGGGATATTCCAGCAGCTGTCAATGCATTCCAGGAAGCAGCT AGTCTTTTAGGTAAGAAGTATGGAGAGACAGCTAATGAGTGTGGAGAAGCCTTCTTTTTCTATGGGAAAT CACTTCTGGAGTTGGCAAGAATGGAGAATGGTGTGTTGGGAAACGCCTTGGAAGGTGTGCATGTGGAAGA GGAAGAAGGAGAAAAAACAGAAGATGAATCTCTGGTAGAAAATAATGATAACATAGATGAGGAAGCAAGG GAAGAGTTGAGAGAACAGGTTTATGACGCCATGGGAGAAAAAGAAGAAGCCAAAAAAACAGAAGACAAGT CTTTGGCAAAGCCTGAAACTGATAAAGAACAGGACAGTGAAATGGAGAAGGGTGGAAGAGAAGATATGGA TATAAGTAAATCTGCAGAGGAGCCACAGGAAAAAGTTGACTTGACTCTAGATTGGTTAACTGAAACCTCT GAAGAGGCAAAAGGAGGAGCAGCACCAGAAGGACCGAATGAAGCTGAGGTCACTTCTGGGAAGCCAGAAC AGGAAGTACCAGATGCTGAGGAAGAAAAATCAGTTTCTGGAACTGATGTCCAAGAAGAGTGCAGAGAAAA AGGAGGTCAGGAGAAGCAGGGAGAGGTAATTGTGAGCATAGAGGAGAAGCCAAAAGAAGTTTCAGAAGAG CAGCCTGTGGTGACTCTAGAAAAGCAGGGCACTGCAGTGGAGGTAGAAGCAGAGTCTTTAGACCCGACAG TCAAGCCAGTGGATGTGGGTGGGGACGAGCCAGAGGAGAAGGTAGTTACCTCTGAAAACGAGGCAGGAAA GGCGGTTCTTGAACAACTGGTAGGTCAAGAAGTACCACCTGCTGAAGAGTCACCAGAGGTGACAACAGAG GCTGCAGAGGCCTCAGCTGTAGAGGCTGGATCAGAAGTCTCTGAAAAGCCTGGGCAGGAGGCTCCAGTTC TCCCTAAGGATGGTGCAGTCAATGGACCGTCAGTTGTAGGAGATCAGACTCCTATTGAACCACAGACTTC TATAGAAAGACTGACAGAAACAAAAGATGGCTCAGGACTAGAGGAGAAGGTCAGGGCAAAGCTGGTTCCT AGTCAGGAGGAGACTAAGCTGTCTGTAGAAGAGTCTGAGGCAGCTGGAGATGGGGTTGATACCAAGGTAG CCCAGGGAGCTACTGAGAAATCACCTGAAGACAAAGTTCAGATAGCTGCTAATGAAGAGACACAAGAGAG AGAAGAACAGATGAAAGAGGGTGAAGAAACTGAAGGCTCAGAAGAGGATGATAAAGAAAATGATAAGACC GAAGAAATGCCAAATGATTCAGTCCTTGAAAACAAGTCTCTTCAAGAAAATGAGGAGGAGGAGATTGGGA ACCTAGAGCTTGCCTGGGATATGCTGGATTTAGCAAAGATCATTTTTAAAAGGCAAGAAACAAAAGAAGC ACAGCTTTATGCTGCCCAGGCACATCTTAAACTCGGAGAAGTTAGTGTTGAATCTGAAAACTATGTGCAA GCTGTGGAGGAGTTCCAGTCCTGCCTTAACCTGCAGGAACAGTACCTGGAAGCCCACGACCGTCTCCTTG CAGAGACCCACTACCAGCTGGGCTTGGCTTATGGGTACAACTCTCAGTATGATGAGGCAGTGGCACAGTT CAGCAAATCTATTGAAGTCATTGAGAACAGAATGGCTGTACTAAACGAGCAGGTGAAGGAGGCTGAAGGA TCGTCTGCTGAATACAAGAAAGAAATTGAGGAACTAAAGGAACTGCTACCCGAAATTAGAGAGAAGATAG AAGATGCAAAGGAGTCTCAGCGTAGTGGGAATGTAGCTGAACTGGCTCTGAAAGCTACTCTGGTGGAGAG TTCTACTTCAGGTTTCACTCCTGGTGGAGGAGGCTCTTCAGTCTCCATGATTGCCAGTAGAAAGCCAACA GACGGTGCTTCCTCATCAAATTGTGTGACTGATATTTCCCACCTTGTCAGAAAGAAGAGGAAACCAGAGG AAGAGAGTCCCCGGAAAGATGATGCAAAGAAAGCCAAACAAGAGCCGGAGGTGAACGGAGGCAGTGGGGA TGCTGTCCCCAGTGGAAATGAAGTTTCGGAAAACATGGAGGAGGAGGCTGAGAATCAGGCTGAAAGCCGG GCAGCAGTGGAGGGGACAGTGGAGGCTGGAGCTACAGTTGAAAGCACTGCATGT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC204052 protein sequence
Red=Cloning site Green=Tags(s) MAMESTATAAVAAELVSADKIEDVPAPSTSADKVESLDVDSEAKKLLGLGQKHLVMGDIPAAVNAFQEAA SLLGKKYGETANECGEAFFFYGKSLLELARMENGVLGNALEGVHVEEEEGEKTEDESLVENNDNIDEEAR EELREQVYDAMGEKEEAKKTEDKSLAKPETDKEQDSEMEKGGREDMDISKSAEEPQEKVDLTLDWLTETS EEAKGGAAPEGPNEAEVTSGKPEQEVPDAEEEKSVSGTDVQEECREKGGQEKQGEVIVSIEEKPKEVSEE QPVVTLEKQGTAVEVEAESLDPTVKPVDVGGDEPEEKVVTSENEAGKAVLEQLVGQEVPPAEESPEVTTE AAEASAVEAGSEVSEKPGQEAPVLPKDGAVNGPSVVGDQTPIEPQTSIERLTETKDGSGLEEKVRAKLVP SQEETKLSVEESEAAGDGVDTKVAQGATEKSPEDKVQIAANEETQEREEQMKEGEETEGSEEDDKENDKT EEMPNDSVLENKSLQENEEEEIGNLELAWDMLDLAKIIFKRQETKEAQLYAAQAHLKLGEVSVESENYVQ AVEEFQSCLNLQEQYLEAHDRLLAETHYQLGLAYGYNSQYDEAVAQFSKSIEVIENRMAVLNEQVKEAEG SSAEYKKEIEELKELLPEIREKIEDAKESQRSGNVAELALKATLVESSTSGFTPGGGGSSVSMIASRKPT DGASSSNCVTDISHLVRKKRKPEEESPRKDDAKKAKQEPEVNGGSGDAVPSGNEVSENMEEEAENQAESR AAVEGTVEAGATVESTAC TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_002482 |
| ORF Size | 2364 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_002482.4 |
| RefSeq Size | 3301 bp |
| RefSeq ORF | 2367 bp |
| Locus ID | 4678 |
| UniProt ID | P49321 |
| Domains | TPR |
| MW | 85.2 kDa |
| Gene Summary | This gene encodes a H1 histone binding protein that is involved in transporting histones into the nucleus of dividing cells. Multiple isoforms are encoded by transcript variants of this gene. The somatic form is expressed in all mitotic cells, is localized to the nucleus, and is coupled to the cell cycle. The testicular form is expressed in embryonic tissues, tumor cells, and the testis. In male germ cells, this protein is localized to the cytoplasm of primary spermatocytes, the nucleus of spermatids, and the periacrosomal region of mature spermatozoa. [provided by RefSeq, Jul 2008] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC204052L1 | Lenti ORF clone of Human nuclear autoantigenic sperm protein (histone-binding) (NASP), transcript variant 2, Myc-DDK-tagged |
CNY 7,700.00 |
|
| RC204052L2 | Lenti ORF clone of Human nuclear autoantigenic sperm protein (histone-binding) (NASP), transcript variant 2, mGFP tagged |
CNY 7,700.00 |
|
| RC204052L3 | Lenti ORF clone of Human nuclear autoantigenic sperm protein (histone-binding) (NASP), transcript variant 2, Myc-DDK-tagged |
CNY 7,700.00 |
|
| RC204052L4 | Lenti ORF clone of Human nuclear autoantigenic sperm protein (histone-binding) (NASP), transcript variant 2, mGFP tagged |
CNY 7,700.00 |
|
| RG204052 | NASP (tGFP-tagged) - Human nuclear autoantigenic sperm protein (histone-binding) (NASP), transcript variant 2 |
CNY 6,370.00 |
|
| SC109476 | NASP (untagged)-Human nuclear autoantigenic sperm protein (histone-binding) (NASP), transcript variant 2 |
CNY 7,220.00 |
|
| SC320902 | NASP (untagged)-Human nuclear autoantigenic sperm protein (histone-binding) (NASP), transcript variant 2 |
CNY 7,220.00 |
