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RAP2B (NM_002886) Human Tagged ORF Clone
CAT＃: RC204287
RAP2B (Myc-DDK-tagged)-Human RAP2B, member of RAS oncogene family (RAP2B)
ORF Plasmid: tGFP
"NM_002886" in other vectors (7)
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CNY 2,400.00
CNY 3,705.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC204287 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGAGAGTACAAAGTGGTGGTGCTGGGCTCGGGCGGCGTGGGCAAGTCCGCGCTCACCGTGCAGTTCG TGACGGGCTCCTTCATCGAGAAGTACGACCCGACCATCGAAGACTTTTACCGCAAGGAGATTGAGGTGGA CTCGTCGCCGTCGGTGCTGGAGATCCTGGATACGGCGGGCACCGAGCAGTTCGCGTCCATGCGGGACCTG TACATCAAGAACGGCCAGGGCTTCATCCTGGTCTACAGCCTCGTCAACCAGCAGAGCTTCCAGGACATCA AGCCCATGCGGGACCAGATCATCCGCGTGAAGCGGTACGAGCGCGTGCCCATGATCCTGGTGGGCAACAA GGTGGACCTGGAGGGTGAGCGCGAGGTCTCGTACGGGGAGGGCAAGGCCCTGGCTGAGGAGTGGAGCTGC CCCTTCATGGAGACGTCGGCCAAAAACAAAGCCTCGGTAGACGAGCTATTTGCCGAGATCGTGCGGCAGA TGAACTACGCGGCGCAGCCCAACGGCGATGAGGGCTGCTGCTCGGCCTGCGTGATCCTC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC204287 protein sequence
Red=Cloning site Green=Tags(s) MREYKVVVLGSGGVGKSALTVQFVTGSFIEKYDPTIEDFYRKEIEVDSSPSVLEILDTAGTEQFASMRDL YIKNGQGFILVYSLVNQQSFQDIKPMRDQIIRVKRYERVPMILVGNKVDLEGEREVSYGEGKALAEEWSC PFMETSAKNKASVDELFAEIVRQMNYAAQPNGDEGCCSACVIL TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_002886 |
| ORF Size | 549 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_002886.4 |
| RefSeq Size | 8413 bp |
| RefSeq ORF | 552 bp |
| Locus ID | 5912 |
| UniProt ID | P61225 |
| Domains | ras, RAS, RHO, RAB |
| Protein Families | Druggable Genome |
| MW | 20.5 kDa |
| Gene Summary | This intronless gene belongs to a family of RAS-related genes. The proteins encoded by these genes share approximately 50% amino acid identity with the classical RAS proteins and have numerous structural features in common. The most striking difference between the RAP and RAS proteins resides in their 61st amino acid: glutamine in RAS is replaced by threonine in RAP proteins. Evidence suggests that this protein may be polyisoprenylated and palmitoylated. [provided by RefSeq, Jul 2008] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC204287L1 | Lenti ORF clone of Human RAP2B, member of RAS oncogene family (RAP2B), Myc-DDK-tagged |
CNY 4,800.00 |
|
| RC204287L2 | Lenti ORF clone of Human RAP2B, member of RAS oncogene family (RAP2B), mGFP tagged |
CNY 5,890.00 |
|
| RC204287L3 | Lenti ORF clone of Human RAP2B, member of RAS oncogene family (RAP2B), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC204287L4 | Lenti ORF clone of Human RAP2B, member of RAS oncogene family (RAP2B), mGFP tagged |
CNY 5,890.00 |
|
| RG204287 | RAP2B (tGFP-tagged) - Human RAP2B, member of RAS oncogene family (RAP2B) |
CNY 4,000.00 |
|
| SC127520 | RAP2B (untagged)-Human RAP2B, member of RAS oncogene family (RAP2B) |
CNY 2,400.00 |
|
| SC320932 | RAP2B (untagged)-Human RAP2B, member of RAS oncogene family (RAP2B) |
CNY 2,400.00 |
