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USP12 (NM_182488) Human Tagged ORF Clone
CAT＃: RC205355
USP12 (Myc-DDK-tagged)-Human ubiquitin specific peptidase 12 (USP12)
ORF Plasmid: tGFP
"NM_182488" in other vectors (6)
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CNY 3,656.00
CNY 3,990.00
Product images
CNY 300.00
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | UBH1; USP12L1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC205355 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAAATCCTAATGACAGTCTCCAAATTCGCCTCCATCTGTACCATGGGCGCCAATGCTTCGGCATTAG AGAAAGAGATTGGTCCAGAACAGTTTCCGGTCAATGAGCACTATTTTGGATTAGTCAATTTTGGGAATAC CTGCTACTGCAATTCAGTTCTTCAAGCACTTTATTTTTGTCGTCCATTTCGGGAAAAAGTTCTTGCGTAT AAGAGTCAACCTAGGAAAAAGGAGAGCCTTCTTACATGCTTAGCAGATCTCTTCCATAGCATAGCCACTC AGAAGAAAAAGGTTGGAGTAATACCCCCTAAGAAGTTCATCACAAGATTACGGAAAGAAAATGAGCTTTT TGACAACTACATGCAACAAGATGCCCATGAATTCTTAAATTACCTACTAAATACAATTGCTGATATTTTA CAAGAAGAGAGAAAGCAGGAAAAACAAAATGGTCGTTTACCTAATGGTAATATTGATAATGAAAATAATA ACAGCACACCAGACCCAACGTGGGTTGATGAGATTTTTCAGGGAACATTAACTAATGAAACCAGATGTCT TACTTGTGAAACTATAAGCAGCAAAGATGAAGATTTTTTAGACCTTTCTGTTGACGTGGAACAAAATACA TCAATTACTCACTGCTTAAGGGGTTTCAGCAACACAGAAACTCTGTGCAGTGAATACAAGTATTACTGTG AAGAGTGTCGCAGCAAACAGGAAGCACACAAACGGATGAAAGTTAAAAAACTGCCCATGATTCTAGCTCT ACACCTGAAGAGATTTAAATATATGGATCAACTTCATCGATATACAAAACTCTCTTACCGGGTAGTTTTT CCTTTAGAACTTCGTCTGTTTAACACTTCAGGTGATGCCACCAATCCAGACAGAATGTACGACCTTGTTG CTGTTGTGGTTCACTGTGGAAGTGGTCCCAATCGAGGCCATTATATTGCAATAGTTAAGAGTCATGATTT TTGGTTGTTGTTTGATGACGACATTGTAGAAAAAATAGATGCACAAGCTATTGAAGAATTCTACGGGTTG ACATCAGATATCTCAAAGAACTCTGAGTCTGGTTACATCCTTTTCTATCAGTCTCGGGAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC205355 protein sequence
Red=Cloning site Green=Tags(s) MEILMTVSKFASICTMGANASALEKEIGPEQFPVNEHYFGLVNFGNTCYCNSVLQALYFCRPFREKVLAY KSQPRKKESLLTCLADLFHSIATQKKKVGVIPPKKFITRLRKENELFDNYMQQDAHEFLNYLLNTIADIL QEERKQEKQNGRLPNGNIDNENNNSTPDPTWVDEIFQGTLTNETRCLTCETISSKDEDFLDLSVDVEQNT SITHCLRGFSNTETLCSEYKYYCEECRSKQEAHKRMKVKKLPMILALHLKRFKYMDQLHRYTKLSYRVVF PLELRLFNTSGDATNPDRMYDLVAVVVHCGSGPNRGHYIAIVKSHDFWLLFDDDIVEKIDAQAIEEFYGL TSDISKNSESGYILFYQSRD TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_182488 |
| ORF Size | 1110 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_182488.2 |
| RefSeq Size | 4503 bp |
| RefSeq ORF | 1113 bp |
| Locus ID | 219333 |
| UniProt ID | O75317 |
| Protein Families | Druggable Genome, Protease |
| MW | 42.8 kDa |
| Gene Summary | Deubiquitinating enzyme. Has almost no deubiquitinating activity by itself and requires the interaction with WDR20 and WDR48 to have a high activity (PubMed:19075014, PubMed:27373336). Not involved in deubiquitination of monoubiquitinated FANCD2 (PubMed:19075014). In complex with WDR48, acts as a potential tumor suppressor by positively regulating PHLPP1 stability (PubMed:24145035).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC205355L1 | Lenti ORF clone of Human ubiquitin specific peptidase 12 (USP12), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC205355L2 | Lenti ORF clone of Human ubiquitin specific peptidase 12 (USP12), mGFP tagged |
CNY 5,890.00 |
|
| RC205355L3 | Lenti ORF clone of Human ubiquitin specific peptidase 12 (USP12), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC205355L4 | Lenti ORF clone of Human ubiquitin specific peptidase 12 (USP12), mGFP tagged |
CNY 5,890.00 |
|
| RG205355 | USP12 (tGFP-tagged) - Human ubiquitin specific peptidase 12 (USP12) |
CNY 4,370.00 |
|
| SC127906 | USP12 (untagged)-Human ubiquitin specific peptidase 12 (USP12) |
CNY 2,950.00 |
