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WWP1 (NM_007013) Human Tagged ORF Clone
CAT＃: RC206243
WWP1 (Myc-DDK-tagged)-Human WW domain containing E3 ubiquitin protein ligase 1 (WWP1)
ORF Plasmid: tGFP
"NM_007013" in other vectors (6)
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CNY 10,128.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | AIP5; hSDRP1; Tiul1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC206243 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCACTGCTTCACCAAGGTCTGATACTAGTAATAACCACAGTGGAAGGTTGCAGTTACAGGTAACTG TTTCTAGTGCCAAACTTAAAAGAAAAAAGAACTGGTTCGGAACAGCAATATATACAGAAGTAGTTGTAGA TGGAGAAATTACGAAAACAGCAAAATCCAGTAGTTCTTCTAATCCAAAATGGGATGAACAGCTAACTGTA AATGTTACGCCACAGACTACATTGGAATTTCAAGTTTGGAGCCATCGCACTTTAAAAGCAGATGCTTTAT TAGGAAAAGCAACGATAGATTTGAAACAAGCTCTGTTGATACACAATAGAAAATTGGAAAGAGTGAAAGA ACAATTAAAACTTTCCTTGGAAAACAAGAATGGCATAGCACAAACTGGTGAATTGACAGTTGTGCTTGAT GGATTGGTGATTGAGCAAGAAAATATAACAAACTGCAGCTCATCTCCAACCATAGAAATACAGGAAAATG GTGATGCCTTACATGAAAATGGAGAGCCTTCAGCAAGGACAACTGCCAGGTTGGCTGTTGAAGGCACGAA TGGAATAGATAATCATGTACCTACAAGCACTCTAGTCCAAAACTCATGCTGCTCGTATGTAGTTAATGGA GACAACACACCTTCATCTCCGTCTCAGGTTGCTGCCAGACCCAAAAATACACCAGCTCCAAAACCACTCG CATCTGAGCCTGCCGATGACACTGTTAATGGAGAATCATCCTCATTTGCACCAACTGATAATGCGTCTGT CACGGGTACTCCAGTAGTGTCTGAAGAAAATGCCTTGTCTCCAAATTGCACTAGTACTACTGTTGAAGAT CCTCCAGTTCAAGAAATACTGACTTCCTCAGAAAACAATGAATGTATTCCTTCTACCAGTGCAGAATTGG AATCTGAAGCTAGAAGTATATTAGAGCCTGACACCTCTAATTCTAGAAGTAGTTCTGCTTTTGAAGCAGC CAAATCAAGACAGCCAGATGGGTGTATGGATCCTGTACGGCAGCAGTCTGGGAATGCCAACACAGAAACC TTGCCATCAGGGTGGGAACAAAGAAAAGATCCTCATGGTAGAACCTATTATGTGGATCATAATACTCGAA CTACCACATGGGAGAGACCACAACCTTTACCTCCAGGTTGGGAAAGAAGAGTTGATGATCGTAGAAGAGT TTATTATGTGGATCATAACACCAGAACAACAACGTGGCAGCGGCCTACCATGGAATCTGTCCGAAATTTT GAACAGTGGCAATCTCAGCGGAACCAATTGCAGGGAGCTATGCAACAGTTTAACCAACGATACCTCTATT CGGCTTCAATGTTAGCTGCAGAAAATGACCCTTATGGACCTTTGCCACCAGGCTGGGAAAAAAGAGTGGA TTCAACAGACAGGGTTTACTTTGTGAATCATAACACAAAAACAACCCAGTGGGAAGATCCAAGAACTCAA GGCTTACAGAATGAAGAACCCCTGCCAGAAGGCTGGGAAATTAGATATACTCGTGAAGGTGTAAGGTACT TTGTTGATCATAACACAAGAACAACAACATTCAAAGATCCTCGCAATGGGAAGTCATCTGTAACTAAAGG TGGTCCACAAATTGCTTATGAACGCGGCTTTAGGTGGAAGCTTGCTCACTTCCGTTATTTGTGCCAGTCT AATGCACTACCTAGTCATGTAAAGATCAATGTGTCCCGGCAGACATTGTTTGAAGATTCCTTCCAACAGA TTATGGCATTAAAACCCTATGACTTGAGGAGGCGCTTATATGTAATATTTAGAGGAGAAGAAGGACTTGA TTATGGTGGCCTAGCGAGAGAATGGTTTTTCTTGCTTTCACATGAAGTTTTGAACCCAATGTATTGCTTA TTTGAGTATGCGGGCAAGAACAACTATTGTCTGCAGATAAATCCAGCATCAACCATTAATCCAGACCATC TTTCATACTTCTGTTTCATTGGTCGTTTTATTGCCATGGCACTATTTCATGGAAAGTTTATCGATACTGG TTTCTCTTTACCATTCTACAAGCGTATGTTAAGTAAAAAACTTACTATTAAGGATTTGGAATCTATTGAT ACTGAATTTTATAACTCCCTTATCTGGATAAGAGATAACAACATTGAAGAATGTGGCTTAGAAATGTACT TTTCTGTTGACATGGAGATTTTGGGAAAAGTTACTTCACATGACCTGAAGTTGGGAGGTTCCAATATTCT GGTGACTGAGGAGAACAAAGATGAATATATTGGTTTAATGACAGAATGGCGTTTTTCTCGAGGAGTACAA GAACAGACCAAAGCTTTCCTTGATGGTTTTAATGAAGTTGTTCCTCTTCAGTGGCTACAGTACTTCGATG AAAAAGAATTAGAGGTTATGTTGTGTGGCATGCAGGAGGTTGACTTGGCAGATTGGCAGAGAAATACTGT TTATCGACATTATACAAGAAACAGCAAGCAAATCATTTGGTTTTGGCAGTTTGTGAAAGAGACAGACAAT GAAGTAAGAATGCGACTATTGCAGTTCGTCACTGGAACCTGCCGTTTACCTCTAGGAGGATTTGCTGAGC TCATGGGAAGTAATGGGCCTCAAAAGTTTTGCATTGAAAAAGTTGGCAAAGACACTTGGTTACCAAGAAG CCATACATGTTTTAATCGCTTGGATCTACCACCATATAAGAGTTATGAACAACTAAAGGAAAAACTTCTT TTTGCAATAGAAGAGACAGAGGGATTTGGACAAGAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC206243 protein sequence
Red=Cloning site Green=Tags(s) MATASPRSDTSNNHSGRLQLQVTVSSAKLKRKKNWFGTAIYTEVVVDGEITKTAKSSSSSNPKWDEQLTV NVTPQTTLEFQVWSHRTLKADALLGKATIDLKQALLIHNRKLERVKEQLKLSLENKNGIAQTGELTVVLD GLVIEQENITNCSSSPTIEIQENGDALHENGEPSARTTARLAVEGTNGIDNHVPTSTLVQNSCCSYVVNG DNTPSSPSQVAARPKNTPAPKPLASEPADDTVNGESSSFAPTDNASVTGTPVVSEENALSPNCTSTTVED PPVQEILTSSENNECIPSTSAELESEARSILEPDTSNSRSSSAFEAAKSRQPDGCMDPVRQQSGNANTET LPSGWEQRKDPHGRTYYVDHNTRTTTWERPQPLPPGWERRVDDRRRVYYVDHNTRTTTWQRPTMESVRNF EQWQSQRNQLQGAMQQFNQRYLYSASMLAAENDPYGPLPPGWEKRVDSTDRVYFVNHNTKTTQWEDPRTQ GLQNEEPLPEGWEIRYTREGVRYFVDHNTRTTTFKDPRNGKSSVTKGGPQIAYERGFRWKLAHFRYLCQS NALPSHVKINVSRQTLFEDSFQQIMALKPYDLRRRLYVIFRGEEGLDYGGLAREWFFLLSHEVLNPMYCL FEYAGKNNYCLQINPASTINPDHLSYFCFIGRFIAMALFHGKFIDTGFSLPFYKRMLSKKLTIKDLESID TEFYNSLIWIRDNNIEECGLEMYFSVDMEILGKVTSHDLKLGGSNILVTEENKDEYIGLMTEWRFSRGVQ EQTKAFLDGFNEVVPLQWLQYFDEKELEVMLCGMQEVDLADWQRNTVYRHYTRNSKQIIWFWQFVKETDN EVRMRLLQFVTGTCRLPLGGFAELMGSNGPQKFCIEKVGKDTWLPRSHTCFNRLDLPPYKSYEQLKEKLL FAIEETEGFGQE TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_007013 |
| ORF Size | 2766 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_007013.4 |
| RefSeq Size | 4120 bp |
| RefSeq ORF | 2769 bp |
| Locus ID | 11059 |
| UniProt ID | Q9H0M0 |
| Domains | C2, HECT, WW |
| Protein Families | Transcription Factors |
| Protein Pathways | Endocytosis, Ubiquitin mediated proteolysis |
| MW | 105.2 kDa |
| Gene Summary | WW domain-containing proteins are found in all eukaryotes and play an important role in the regulation of a wide variety of cellular functions such as protein degradation, transcription, and RNA splicing. This gene encodes a protein which contains 4 tandem WW domains and a HECT (homologous to the E6-associated protein carboxyl terminus) domain. The encoded protein belongs to a family of NEDD4-like proteins, which are E3 ubiquitin-ligase molecules and regulate key trafficking decisions, including targeting of proteins to proteosomes or lysosomes. Alternative splicing of this gene generates at least 6 transcript variants; however, the full length nature of these transcripts has not been defined. [provided by RefSeq, Jul 2008] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC206243L1 | Lenti ORF clone of Human WW domain containing E3 ubiquitin protein ligase 1 (WWP1), Myc-DDK-tagged |
CNY 12,528.00 |
|
| RC206243L2 | Lenti ORF clone of Human WW domain containing E3 ubiquitin protein ligase 1 (WWP1), mGFP tagged |
CNY 5,890.00 |
|
| RC206243L3 | Lenti ORF clone of Human WW domain containing E3 ubiquitin protein ligase 1 (WWP1), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC206243L4 | Lenti ORF clone of Human WW domain containing E3 ubiquitin protein ligase 1 (WWP1), mGFP tagged |
CNY 12,528.00 |
|
| RG206243 | WWP1 (tGFP-tagged) - Human WW domain containing E3 ubiquitin protein ligase 1 (WWP1) |
CNY 11,728.00 |
|
| SC112699 | WWP1 (untagged)-Human WW domain containing E3 ubiquitin protein ligase 1 (WWP1) |
CNY 10,136.00 |
