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DDAH1 (NM_012137) Human Tagged ORF Clone
CAT＃: RC207548
DDAH1 (Myc-DDK-tagged)-Human dimethylarginine dimethylaminohydrolase 1 (DDAH1), transcript variant 1
ORF Plasmid: tGFP
"NM_012137" in other vectors (6)
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CNY 2,400.00
CNY 3,990.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | DDAH; DDAH-1; DDAHI; HEL-S-16 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC207548 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCGGGCTCGGCCACCCCGCCGCCTTCGGCCGGGCCACCCACGCCGTGGTGCGGGCGCTACCCGAGT CGCTCGGCCAGCACGCGCTGAGAAGCGCCAAGGGCGAGGAGGTGGACGTCGCCCGCGCGGAACGGCAGCA CCAGCTCTACGTGGGCGTGCTGGGCAGCAAGCTGGGGCTGCAGGTGGTGGAGCTGCCGGCCGACGAGAGC CTTCCGGACTGCGTCTTCGTGGAGGACGTGGCCGTGGTGTGCGAGGAGACGGCCCTCATCACCCGACCCG GGGCGCCGAGCCGGAGGAAGGAGGTTGACATGATGAAAGAAGCATTAGAAAAACTTCAGCTCAATATAGT AGAGATGAAAGATGAAAATGCAACTTTAGATGGCGGAGATGTTTTATTCACAGGCAGAGAATTTTTTGTG GGCCTTTCCAAAAGGACAAATCAACGAGGTGCTGAAATCTTGGCTGATACTTTTAAGGACTATGCAGTCT CCACAGTGCCAGTGGCAGATGGGTTGCATTTGAAGAGTTTCTGCAGCATGGCTGGGCCTAACCTGATCGC AATTGGGTCTAGTGAATCTGCACAGAAGGCCCTTAAGATCATGCAACAGATGAGTGACCACCGCTACGAC AAACTCACTGTGCCTGATGACATAGCAGCAAACTGTATATATCTAAATATCCCCAACAAAGGGCACGTCT TGCTGCACCGAACCCCGGAAGAGTATCCAGAAAGTGCAAAGGTTTATGAGAAACTGAAGGACCATATGCT GATCCCCGTGAGCATGTCTGAACTGGAAAAGGTGGATGGGCTGCTCACCTGCTGCTCAGTTTTAATTAAC AAGAAAGTAGACTCC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC207548 protein sequence
Red=Cloning site Green=Tags(s) MAGLGHPAAFGRATHAVVRALPESLGQHALRSAKGEEVDVARAERQHQLYVGVLGSKLGLQVVELPADES LPDCVFVEDVAVVCEETALITRPGAPSRRKEVDMMKEALEKLQLNIVEMKDENATLDGGDVLFTGREFFV GLSKRTNQRGAEILADTFKDYAVSTVPVADGLHLKSFCSMAGPNLIAIGSSESAQKALKIMQQMSDHRYD KLTVPDDIAANCIYLNIPNKGHVLLHRTPEEYPESAKVYEKLKDHMLIPVSMSELEKVDGLLTCCSVLIN KKVDS TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_012137 |
| ORF Size | 855 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_012137.4 |
| RefSeq Size | 3991 bp |
| RefSeq ORF | 858 bp |
| Locus ID | 23576 |
| UniProt ID | O94760 |
| Domains | Amidinotransf |
| MW | 31.1 kDa |
| Gene Summary | This gene belongs to the dimethylarginine dimethylaminohydrolase (DDAH) gene family. The encoded enzyme plays a role in nitric oxide generation by regulating cellular concentrations of methylarginines, which in turn inhibit nitric oxide synthase activity. [provided by RefSeq, Jul 2008] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC207548L1 | Lenti ORF clone of Human dimethylarginine dimethylaminohydrolase 1 (DDAH1), transcript variant 1, Myc-DDK-tagged |
CNY 4,800.00 |
|
| RC207548L2 | Lenti ORF clone of Human dimethylarginine dimethylaminohydrolase 1 (DDAH1), transcript variant 1, mGFP tagged |
CNY 5,890.00 |
|
| RC207548L3 | Lenti ORF clone of Human dimethylarginine dimethylaminohydrolase 1 (DDAH1), transcript variant 1, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC207548L4 | Lenti ORF clone of Human dimethylarginine dimethylaminohydrolase 1 (DDAH1), transcript variant 1, mGFP tagged |
CNY 5,890.00 |
|
| RG207548 | DDAH1 (tGFP-tagged) - Human dimethylarginine dimethylaminohydrolase 1 (DDAH1), transcript variant 1 |
CNY 4,000.00 |
|
| SC321756 | DDAH1 (untagged)-Human dimethylarginine dimethylaminohydrolase 1 (DDAH1), transcript variant 1 |
CNY 2,400.00 |
