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SPARCL1 (NM_004684) Human Tagged ORF Clone
CAT＃: RC207583
SPARCL1 (Myc-DDK-tagged)-Human SPARC-like 1 (hevin) (SPARCL1), transcript variant 2
ORF Plasmid: tGFP
"NM_004684" in other vectors (6)
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CNY 4,560.00
| Cited in 1 publication. |
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | MAST 9; MAST9; PIG33; SC1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC207583 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAAGACTGGGCTTTTTTTCCTATGTCTCTTGGGAACTGCAGCTGCAATCCCGACAAATGCAAGATTAT TATCTGATCATTCCAAACCAACTGCTGAAACGGTAGCACCCGACAACACTGCAATCCCCAGTTTAAGGGC TGAAGATGAAGAAAATGAAAAAGAAACAGCAGTATCCACAGAAGACGATTCCCACCATAAGGCTGAAAAA TCATCAGTACTAAAGTCAAAAGAGGAAAGCCATGAACAGTCAGCAGAACAGGGCAAGAGTTCTAGCCAAG AGCTGGGATTGAAGGATCAAGAGGACAGTGATGGTGACTTAAGTGTGAATTTGGAGTATGCACCAACTGA AGGTACATTGGACATAAAAGAAGATATGAGTGAGCCTCAGGAGAAAAAACTCTCAGAGAACACTGATTTT TTGGCTCCTGGTGTTAGTTCCTTCACAGATTCTAACCAACAAGAAAGTATCACAAAGAGAGAGGAAAACC AAGAACAACCTAGAAATTATTCACATCATCAGTTGAACAGGAGCAGTAAACATAGCCAAGGCCTAAGGGA TCAAGGAAACCAAGAGCAGGATCCAAATATTTCCAATGGAGAAGAGGAAGAAGAAAAAGAGCCAGGTGAA GTTGGTACCCACAATGATAACCAAGAAAGAAAGACAGAATTGCCCAGGGAGCATGCTAACAGCAAGCAGG AGGAAGACAATACCCAATCTGATGATATTTTGGAAGAGTCTGATCAACCAACTCAAGTAAGCAAGATGCA GGAGGATGAATTTGATCAGGGTAACCAAGAACAAGAAGATAACTCCAATGCAGAAATGGAAGAGGAAAAT GCATCGAACGTCAATAAGCACATTCAAGAAACTGAATGGCAGAGTCAAGAGGGTAAAACTGGCCTAGAAG CTATCAGCAACCACAAAGAGACAGAAGAAAAGACTGTTTCTGAGGCTCTGCTCATGGAACCTACTGATGA TGGTAATACCACGCCCAGAAATCATGGAGTTGATGATGATGGCGATGATGATGGCGATGATGGCGGCACT GATGGCCCCAGGCACAGTGCAAGTGATGACTACTTCATCCCAAGCCAGGCCTTTCTGGAGGCCGAGAGAG CTCAATCCATTGCCTATCACCTCAAAATTGAGGAGCAAAGAGAAAAAGTACATGAAAATGAAAATATAGG TACCACTGAGCCTGGAGAGCACCAAGAGGCCAAGAAAGCAGAGAACTCATCAAATGAGGAGGAAACGTCA AGTGAAGGCAACATGAGGGTGCATGCTGTGGATTCTTGCATGAGCTTCCAGTGTAAAAGAGGCCACATCT GTAAGGCAGACCAACAGGGAAAACCTCACTGTGTCTGCCAGGATCCAGTGACTTGTCCTCCAACAAAACC CCTTGATCAAGTTTGTGGCACTGACAATCAGACCTATGCTAGTTCCTGTCATCTATTCGCTACTAAATGC AGACTGGAGGGGACCAAAAAGGGGCATCAACTCCAGCTGGATTATTTTGGAGCCTGCAAATCTATTCCTA CTTGTACGGACTTTGAAGTGATTCAGTTTCCTCTACGGATGAGAGACTGGCTCAAGAATATCCTCATGCA GCTTTATGAAGCCAACTCTGAACACGCTGGTTATCTAAATGAGAAGCAGAGAAATAAAGTCAAGAAAATT TACCTGGATGAAAAGAGGCTTTTGGCTGGGGACCATCCCATTGACCTTCTCTTAAGGGACTTTAAGAAAA ACTACCACATGTATGTGTATCCTGTGCACTGGCAGTTTAGTGAACTTGACCAACACCCTATGGATAGAGT CTTGACACATTCTGAACTTGCTCCTCTGCGAGCATCTCTGGTGCCCATGGAACACTGCATAACCCGTTTC TTTGAGGAGTGTGACCCCAACAAGGATAAGCACATCACCCTGAAGGAGTGGGGCCACTGCTTTGGAATTA AAGAAGAGGACATAGATGAAAATCTCTTGTTT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC207583 protein sequence
Red=Cloning site Green=Tags(s) MKTGLFFLCLLGTAAAIPTNARLLSDHSKPTAETVAPDNTAIPSLRAEDEENEKETAVSTEDDSHHKAEK SSVLKSKEESHEQSAEQGKSSSQELGLKDQEDSDGDLSVNLEYAPTEGTLDIKEDMSEPQEKKLSENTDF LAPGVSSFTDSNQQESITKREENQEQPRNYSHHQLNRSSKHSQGLRDQGNQEQDPNISNGEEEEEKEPGE VGTHNDNQERKTELPREHANSKQEEDNTQSDDILEESDQPTQVSKMQEDEFDQGNQEQEDNSNAEMEEEN ASNVNKHIQETEWQSQEGKTGLEAISNHKETEEKTVSEALLMEPTDDGNTTPRNHGVDDDGDDDGDDGGT DGPRHSASDDYFIPSQAFLEAERAQSIAYHLKIEEQREKVHENENIGTTEPGEHQEAKKAENSSNEEETS SEGNMRVHAVDSCMSFQCKRGHICKADQQGKPHCVCQDPVTCPPTKPLDQVCGTDNQTYASSCHLFATKC RLEGTKKGHQLQLDYFGACKSIPTCTDFEVIQFPLRMRDWLKNILMQLYEANSEHAGYLNEKQRNKVKKI YLDEKRLLAGDHPIDLLLRDFKKNYHMYVYPVHWQFSELDQHPMDRVLTHSELAPLRASLVPMEHCITRF FEECDPNKDKHITLKEWGHCFGIKEEDIDENLLF TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_004684 |
| ORF Size | 1992 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_004684.5 |
| RefSeq Size | 2910 bp |
| RefSeq ORF | 1995 bp |
| Locus ID | 8404 |
| UniProt ID | Q14515 |
| Domains | kazal, FOLN |
| Protein Families | Druggable Genome, Secreted Protein |
| MW | 75.2 kDa |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
SPARCL1 suppresses metastasis in prostate cancer
,null,
Molecular Oncology
,PubMed ID 23916135
[SPARCL1]
|
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC207583L1 | Lenti ORF clone of Human SPARC-like 1 (hevin) (SPARCL1), transcript variant 2, Myc-DDK-tagged |
CNY 6,460.00 |
|
| RC207583L2 | Lenti ORF clone of Human SPARC-like 1 (hevin) (SPARCL1), transcript variant 2, mGFP tagged |
CNY 6,460.00 |
|
| RC207583L3 | Lenti ORF clone of Human SPARC-like 1 (hevin) (SPARCL1), transcript variant 2, Myc-DDK-tagged |
CNY 6,460.00 |
|
| RC207583L4 | Lenti ORF clone of Human SPARC-like 1 (hevin) (SPARCL1), transcript variant 2, mGFP tagged |
CNY 6,460.00 |
|
| RG207583 | SPARCL1 (tGFP-tagged) - Human SPARC-like 1 (hevin) (SPARCL1), transcript variant 2 |
CNY 5,040.00 |
|
| SC321726 | SPARCL1 (untagged)-Human SPARC-like 1 (hevin) (SPARCL1), transcript variant 2 |
CNY 2,950.00 |
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