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MTRF1 (NM_004294) Human Tagged ORF Clone
CAT＃: RC207912
MTRF1 (Myc-DDK-tagged)-Human mitochondrial translational release factor 1 (MTRF1), nuclear gene encoding mitochondrial protein
ORF Plasmid: tGFP
"NM_004294" in other vectors (4)
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CNY 3,656.00
CNY 3,990.00
Product images
CNY 300.00
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | MRF1; MTTRF1; RF1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC207912 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAATCGTCACCTGTGTGTTTGGCTTTTTAGACATCCATCTCTTAATGGTTACCTCCAGTGTCACATCC AGCTCCATTCTCATCAATTTAGACAGATACATCTTGATACAAGGCTGCAAGTTTTTAGACAAAACAGGAA TTGCATTCTTCATCTGTTAAGTAAGAATTGGTCCAGGAGATATTGCCATCAAGACACCAAGATGCTCTGG AAGCATAAAGCACTACAGAAATATATGGAGAACCTGAGTAAGGAGTACCAAACACTTGAGCAATGTCTGC AGCATATCCCTGTGAATGAAGAAAACCGAAGGTCCTTGAACAGAAGGCATGCTGAGTTGGCACCTCTTGC AGCCATTTACCAAGAAATTCAGGAGACTGAACAAGCAATTGAAGAATTAGAATCAATGTGTAAAAGCCTA AATAAACAAGATGAAAAGCAGTTACAAGAACTTGCACTGGAAGAAAGGCAAACCATTGATCAAAAAATCA ACATGTTGTACAATGAGCTTTTCCAGAGCCTTGTGCCAAAGGAGAAATATGACAAAAATGATGTTATTTT AGAGGTGACAGCTGGAAGGACTACTGGAGGTGACATCTGCCAACAATTTACCCGAGAAATATTTGACATG TACCAGAATTATTCGTGCTATAAACACTGGCAATTTGAACTTCTGAATTATACACCAGCAGATTATGGTG GACTACATCATGCAGCCGCCCGAATTTCCGGTGACGGTGTCTATAAGCATTTGAAGTATGAGGGTGGGAT TCACCGAGTTCAGCGCATCCCCGAGGTGGGCCTGTCCTCAAGGATGCAGCGCATTCACACAGGAACGATG TCGGTTATTGTCCTTCCTCAGCCAGATGAGGTGGATGTGAAATTGGACCCCAAGGATTTGCGAATAGATA CATTTCGAGCCAAAGGAGCAGGAGGGCAGCATGTTAATAAAACTGATAGTGCCGTCAGACTTGTCCACAT CCCCACAGGGCTAGTAGTAGAATGCCAACAAGAAAGATCACAGATAAAAAATAAAGAAATAGCCTTTCGT GTGTTGAGAGCTAGACTCTACCAGCAGATTATTGAGAAAGACAAGCGTCAGCAACAAAGTGCTAGAAAAC TGCAGGTGGGAACAAGAGCCCAGTCAGAGCGAATTCGGACATATAATTTCACCCAGGATAGAGTCAGTGA CCACAGGATAGCATATGAAGTTCGTGATATTAAGGAATTTTTATGTGGTGGGAAGGGCCTGGATCAGCTA ATTCAGAGACTGCTTCAATCAGCAGATGAAGAAGCCATTGCTGAACTTTTGGATGAACACCTTAAATCAG CAAAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC207912 protein sequence
Red=Cloning site Green=Tags(s) MNRHLCVWLFRHPSLNGYLQCHIQLHSHQFRQIHLDTRLQVFRQNRNCILHLLSKNWSRRYCHQDTKMLW KHKALQKYMENLSKEYQTLEQCLQHIPVNEENRRSLNRRHAELAPLAAIYQEIQETEQAIEELESMCKSL NKQDEKQLQELALEERQTIDQKINMLYNELFQSLVPKEKYDKNDVILEVTAGRTTGGDICQQFTREIFDM YQNYSCYKHWQFELLNYTPADYGGLHHAAARISGDGVYKHLKYEGGIHRVQRIPEVGLSSRMQRIHTGTM SVIVLPQPDEVDVKLDPKDLRIDTFRAKGAGGQHVNKTDSAVRLVHIPTGLVVECQQERSQIKNKEIAFR VLRARLYQQIIEKDKRQQQSARKLQVGTRAQSERIRTYNFTQDRVSDHRIAYEVRDIKEFLCGGKGLDQL IQRLLQSADEEAIAELLDEHLKSAK TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_004294 |
| ORF Size | 1335 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_004294.4 |
| RefSeq Size | 2174 bp |
| RefSeq ORF | 1338 bp |
| Locus ID | 9617 |
| UniProt ID | O75570 |
| MW | 52.3 kDa |
| Gene Summary | The protein encoded by this gene was determined by in silico methods to be a mitochondrial protein with similarity to the peptide chain release factors (RFs) discovered in bacteria and yeast. The peptide chain release factors direct the termination of translation in response to the peptide chain termination codons. Initially thought to have a role in the termination of mitochondria protein synthesis, a recent publication found no mitochondrial translation release functionality. Multiple alternatively spliced transcript variants have been suggested by mRNA and EST data; however, their full-length natures are not clear. [provided by RefSeq, Jul 2008] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC207912L3 | Lenti ORF clone of Human mitochondrial translational release factor 1 (MTRF1), nuclear gene encoding mitochondrial protein, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC207912L4 | Lenti ORF clone of Human mitochondrial translational release factor 1 (MTRF1), nuclear gene encoding mitochondrial protein, mGFP tagged |
CNY 5,890.00 |
|
| RG207912 | MTRF1 (tGFP-tagged) - Human mitochondrial translational release factor 1 (MTRF1), nuclear gene encoding mitochondrial protein |
CNY 4,370.00 |
|
| SC303463 | MTRF1 (untagged)-Human mitochondrial translational release factor 1 (MTRF1), nuclear gene encoding mitochondrial protein |
CNY 7,220.00 |
