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VPS4B (NM_004869) Human Tagged ORF Clone
CAT＃: RC208019
VPS4B (Myc-DDK-tagged)-Human vacuolar protein sorting 4 homolog B (S. cerevisiae) (VPS4B)
ORF Plasmid: tGFP
"NM_004869" in other vectors (7)
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CNY 3,656.00
CNY 3,990.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | MIG1; SKD1; SKD1B; VPS4-2 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC208019 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCATCCACTTCGCCCAACCTCCAGAAAGCGATAGATCTGGCTAGCAAAGCAGCGCAAGAAGACAAGG CTGGGAACTACGAAGAAGCCCTTCAGCTCTATCAGCATGCTGTGCAGTATTTTCTTCATGTCGTTAAATA TGAAGCACAGGGTGATAAAGCCAAGCAAAGTATCAGGGCAAAGTGTACAGAATATCTTGATAGAGCAGAA AAACTAAAGGAGTACCTGAAAAATAAAGAGAAAAAAGCACAGAAGCCAGTGAAAGAAGGACAGCCGAGTC CAGCAGATGAGAAGGGGAATGACAGTGATGGGGAAGGAGAATCTGATGATCCTGAAAAAAAGAAACTACA GAATCAACTTCAAGGTGCCATTGTTATAGAACGACCAAATGTGAAATGGAGTGACGTTGCTGGACTTGAA GGAGCCAAAGAAGCACTGAAAGAGGCTGTGATACTGCCTATTAAATTTCCTCATCTTTTTACAGGCAAGA GAACACCTTGGAGGGGAATCCTATTATTTGGGCCGCCTGGAACAGGAAAGTCCTACTTAGCCAAAGCTGT AGCAACAGAAGCCAACAACTCAACATTTTTTTCAATATCTTCCTCTGATCTTGTTTCTAAGTGGCTAGGT GAAAGTGAAAAACTGGTTAAGAATTTATTCCAACTTGCCAGAGAGAACAAGCCCTCCATTATCTTCATTG ATGAAATTGATTCTCTCTGTGGTTCAAGAAGTGAAAATGAAAGTGAAGCCGCACGTAGAATTAAGACGGA GTTCCTAGTGCAAATGCAAGGGGTTGGTGTAGACAATGATGGAATTTTGGTTCTGGGAGCTACAAATATA CCCTGGGTTCTGGATTCTGCCATTAGGCGAAGATTTGAGAAACGAATTTATATTCCCTTGCCGGAACCCC ATGCCCGAGCAGCAATGTTTAAACTGCACCTAGGGACCACTCAGAACAGTCTCACGGAAGCAGACTTTCG GGAACTTGGGAGGAAAACAGATGGTTATTCAGGGGCAGATATAAGTATCATTGTACGTGATGCCCTTATG CAGCCTGTTAGGAAAGTACAGTCAGCTACTCATTTTAAAAAGGTTCGCGGACCTTCCCGAGCTGATCCTA ACCATCTTGTAGATGATCTGCTAACACCTTGCTCTCCAGGTGACCCTGGTGCCATTGAAATGACATGGAT GGATGTCCCTGGAGATAAACTTTTGGAGCCAGTTGTTTCCATGTCGGATATGTTGCGGTCACTATCTAAC ACAAAACCTACAGTCAATGAACATGACTTGTTGAAATTAAAGAAGTTTACAGAAGATTTTGGTCAAGAAG GC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC208019 protein sequence
Red=Cloning site Green=Tags(s) MSSTSPNLQKAIDLASKAAQEDKAGNYEEALQLYQHAVQYFLHVVKYEAQGDKAKQSIRAKCTEYLDRAE KLKEYLKNKEKKAQKPVKEGQPSPADEKGNDSDGEGESDDPEKKKLQNQLQGAIVIERPNVKWSDVAGLE GAKEALKEAVILPIKFPHLFTGKRTPWRGILLFGPPGTGKSYLAKAVATEANNSTFFSISSSDLVSKWLG ESEKLVKNLFQLARENKPSIIFIDEIDSLCGSRSENESEAARRIKTEFLVQMQGVGVDNDGILVLGATNI PWVLDSAIRRRFEKRIYIPLPEPHARAAMFKLHLGTTQNSLTEADFRELGRKTDGYSGADISIIVRDALM QPVRKVQSATHFKKVRGPSRADPNHLVDDLLTPCSPGDPGAIEMTWMDVPGDKLLEPVVSMSDMLRSLSN TKPTVNEHDLLKLKKFTEDFGQEG TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_004869 |
| ORF Size | 1332 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_004869.4 |
| RefSeq Size | 3396 bp |
| RefSeq ORF | 1335 bp |
| Locus ID | 9525 |
| UniProt ID | O75351 |
| Domains | AAA, AAA, MIT |
| Protein Families | Transcription Factors |
| Protein Pathways | Endocytosis |
| MW | 49.3 kDa |
| Gene Summary | The protein encoded by this gene is a member of the AAA protein family (ATPases associated with diverse cellular activities), and is the homolog of the yeast Vps4 protein. In humans, two paralogs of the yeast protein have been identified. The former share a high degree of aa sequence similarity with each other, and also with yeast Vps4 and mouse Skd1 proteins. Mouse Skd1 (suppressor of K+ transport defect 1) has been shown to be a yeast Vps4 ortholog. Functional studies indicate that both human paralogs associate with the endosomal compartments, and are involved in intracellular protein trafficking, similar to Vps4 protein in yeast. The gene encoding this paralog has been mapped to chromosome 18; the gene for the other resides on chromosome 16. [provided by RefSeq, Jul 2008] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC208019L1 | Lenti ORF clone of Human vacuolar protein sorting 4 homolog B (S. cerevisiae) (VPS4B), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC208019L2 | Lenti ORF clone of Human vacuolar protein sorting 4 homolog B (S. cerevisiae) (VPS4B), mGFP tagged |
CNY 5,890.00 |
|
| RC208019L3 | Lenti ORF clone of Human vacuolar protein sorting 4 homolog B (S. cerevisiae) (VPS4B), Myc-DDK-tagged |
CNY 6,056.00 |
|
| RC208019L4 | Lenti ORF clone of Human vacuolar protein sorting 4 homolog B (S. cerevisiae) (VPS4B), mGFP tagged |
CNY 5,890.00 |
|
| RG208019 | VPS4B (tGFP-tagged) - Human vacuolar protein sorting 4 homolog B (S. cerevisiae) (VPS4B) |
CNY 4,370.00 |
|
| SC127708 | VPS4B (untagged)-Human vacuolar protein sorting 4 homolog B (S. cerevisiae) (VPS4B) |
CNY 2,950.00 |
|
| SC324083 | VPS4B (untagged)-Human vacuolar protein sorting 4 homolog B (S. cerevisiae) (VPS4B) |
CNY 3,656.00 |
