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WIPI1 (NM_017983) Human Tagged ORF Clone
CAT＃: RC208094
WIPI1 (Myc-DDK-tagged)-Human WD repeat domain, phosphoinositide interacting 1 (WIPI1)
ORF Plasmid: tGFP
"NM_017983" in other vectors (7)
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CNY 3,656.00
CNY 3,990.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | ATG18; ATG18A; WIPI49 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC208094 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGGCCGAGGCCGCGGACGCTCCCCCGGGCGGGGTTGAGTCGGCGCTCAGCTGCTTCTCTTTCAACC AGGACTGCACATCCCTAGCAATTGGAACTAAAGCCGGGTATAAGCTGTTTTCTCTGAGTTCTGTGGAGCA GCTGGATCAAGTCCACGGAAGCAATGAAATCCCGGACGTCTACATCGTGGAGCGCCTCTTCTCCAGCAGC CTGGTGGTGGTAGTCAGTCACACAAAACCACGGCAGATGAACGTGTATCACTTCAAGAAAGGCACAGAGA TCTGTAATTACAGCTACTCCAGCAACATCTTGTCCATAAGGCTGAACCGGCAAAGGCTGCTGGTTTGCCT AGAAGAGTCCATTTATATTCACAACATTAAAGACATGAAGCTGTTGAAGACCCTCCTGGATATTCCTGCA AACCCAACAGGTCTATGTGCTCTCTCTATCAACCATTCCAATTCTTACCTGGCCTATCCTGGAAGCCTGA CTTCAGGGGAGATTGTGCTTTATGATGGAAACTCCCTGAAAACAGTCTGCACTATTGCTGCCCATGAGGG AACACTAGCTGCCATCACCTTCAATGCCTCAGGCTCCAAACTAGCAAGTGCGTCTGAAAAAGGCACAGTC ATCCGGGTGTTCTCTGTCCCTGATGGGCAAAAGCTCTATGAGTTCCGGAGAGGGATGAAAAGGTATGTGA CAATCAGCTCTCTAGTGTTCAGTATGGATTCACAATTCCTCTGCGCCTCCAGTAACACCGAGACGGTACA CATCTTCAAGCTGGAACAGGTCACCAACAGTCGACCAGAAGAGCCTTCGACCTGGAGTGGCTACATGGGA AAGATGTTTATGGCTGCTACCAACTACCTCCCTACCCAGGTGTCAGACATGATGCATCAGGACAGGGCTT TTGCCACTGCACGCTTGAACTTCTCCGGACAGAGGAACATCTGTACCCTCTCAACGATCCAGAAGTTGCC ACGGCTGCTAGTTGCGTCATCCAGTGGACACCTTTATATGTACAATTTGGATCCTCAGGATGGAGGAGAG TGTGTCTTAATCAAAACCCACAGCTTGCTTGGCTCAGGAACAACAGAAGAGAATAAAGAAAATGACCTCA GACCTTCCTTACCTCAGTCTTATGCAGCGACCGTAGCCAGACCAAGTGCATCTTCAGCCTCCACGGTGCC AGGTTATTCTGAGGACGGCGGGGCGCTGCGAGGAGAAGTTATTCCTGAACATGAGTTTGCGACGGGACCA GTGTGTCTTGATGATGAGAATGAGTTTCCTCCTATAATCTTGTGCCGTGGAAATCAGAAGGGCAAAACGA AGCAGTCA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC208094 protein sequence
Red=Cloning site Green=Tags(s) MEAEAADAPPGGVESALSCFSFNQDCTSLAIGTKAGYKLFSLSSVEQLDQVHGSNEIPDVYIVERLFSSS LVVVVSHTKPRQMNVYHFKKGTEICNYSYSSNILSIRLNRQRLLVCLEESIYIHNIKDMKLLKTLLDIPA NPTGLCALSINHSNSYLAYPGSLTSGEIVLYDGNSLKTVCTIAAHEGTLAAITFNASGSKLASASEKGTV IRVFSVPDGQKLYEFRRGMKRYVTISSLVFSMDSQFLCASSNTETVHIFKLEQVTNSRPEEPSTWSGYMG KMFMAATNYLPTQVSDMMHQDRAFATARLNFSGQRNICTLSTIQKLPRLLVASSSGHLYMYNLDPQDGGE CVLIKTHSLLGSGTTEENKENDLRPSLPQSYAATVARPSASSASTVPGYSEDGGALRGEVIPEHEFATGP VCLDDENEFPPIILCRGNQKGKTKQS TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_017983 |
| ORF Size | 1338 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_017983.4 |
| RefSeq Size | 1939 bp |
| RefSeq ORF | 1341 bp |
| Locus ID | 55062 |
| UniProt ID | Q5MNZ9 |
| Domains | WD40 |
| MW | 48.7 kDa |
| Gene Summary | This gene encodes a WD40 repeat protein. Members of the WD40 repeat family are key components of many essential biologic functions. They regulate the assembly of multiprotein complexes by presenting a beta-propeller platform for simultaneous and reversible protein-protein interactions. Members of the WIPI subfamily of WD40 repeat proteins have a 7-bladed propeller structure and contain a conserved motif for interaction with phospholipids. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2016] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC208094L1 | Lenti ORF clone of Human WD repeat domain, phosphoinositide interacting 1 (WIPI1), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC208094L2 | Lenti ORF clone of Human WD repeat domain, phosphoinositide interacting 1 (WIPI1), mGFP tagged |
CNY 5,890.00 |
|
| RC208094L3 | Lenti ORF clone of Human WD repeat domain, phosphoinositide interacting 1 (WIPI1), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC208094L4 | Lenti ORF clone of Human WD repeat domain, phosphoinositide interacting 1 (WIPI1), mGFP tagged |
CNY 5,890.00 |
|
| RG208094 | WIPI1 (tGFP-tagged) - Human WD repeat domain, phosphoinositide interacting 1 (WIPI1) |
CNY 4,370.00 |
|
| SC310381 | WIPI1 (untagged)-Human WD repeat domain, phosphoinositide interacting 1 (WIPI1) |
CNY 3,656.00 |
|
| SC322622 | WIPI1 (untagged)-Human WD repeat domain, phosphoinositide interacting 1 (WIPI1) |
CNY 3,656.00 |
