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VPS4A (NM_013245) Human Tagged ORF Clone
CAT＃: RC208099
VPS4A (Myc-DDK-tagged)-Human vacuolar protein sorting 4 homolog A (S. cerevisiae) (VPS4A)
ORF Plasmid: tGFP
"NM_013245" in other vectors (6)
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CNY 3,656.00
CNY 3,990.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | CIMDAG; SKD1; SKD1A; SKD2; VPS4; VPS4-1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC208099 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACAACGTCAACCCTCCAGAAAGCCATTGATCTGGTGACGAAAGCCACAGAGGAGGACAAAGCCAAGA ACTACGAGGAGGCGCTGCGGCTGTACCAGCATGCGGTGGAGTACTTCCTCCACGCTATCAAGTATGAGGC CCACAGCGACAAGGCCAAGGAGAGCATTCGAGCCAAGTGCGTGCAGTACCTAGACCGGGCCGAGAAGCTG AAGGATTATTTACGAAGCAAAGAGAAACACGGCAAGAAGCCAGTCAAAGAGAACCAGAGTGAGGGCAAGG GCAGTGACAGTGACAGTGAAGGGGATAATCCGGAGAAAAAGAAACTGCAAGAACAGCTGATGGGTGCCGT CGTGATGGAGAAGCCCAACATACGGTGGAACGACGTGGCCGGGCTGGAGGGGGCCAAGGAGGCCCTCAAA GAAGCTGTCATTTTGCCAATCAAATTCCCACACTTGTTCACAGGCAAGCGCACCCCCTGGCGGGGGATTC TGCTGTTCGGACCCCCTGGCACAGGGAAATCCTACCTGGCCAAAGCCGTGGCAACAGAGGCCAACAACTC CACCTTCTTCTCTGTGTCCTCCTCAGATCTGATGTCCAAGTGGCTGGGGGAGAGTGAAAAGCTGGTCAAG AACCTGTTTGAGCTGGCCAGGCAGCACAAGCCCTCCATCATCTTCATCGATGAGGTGGATTCCCTCTGCG GGTCCCGAAATGAAAATGAGAGTGAGGCCGCCCGGAGGATCAAAACGGAGTTCTTGGTCCAGATGCAGGG GGTGGGGAATAACAATGATGGGACTCTGGTTCTTGGAGCCACAAACATCCCATGGGTGTTGGATTCGGCC ATCAGGAGGAGGTTTGAAAAACGAATTTATATCCCCTTGCCGGAGGAAGCTGCCCGCGCCCAGATGTTCC GGTTGCATCTCGGGAGCACTCCCCACAACCTCACGGATGCAAACATCCACGAGCTGGCCCGGAAGACGGA AGGCTACTCGGGCGCGGACATCAGCATCATCGTGCGGGACTCTCTCATGCAGCCCGTGAGGAAGGTGCAG TCGGCCACACACTTCAAAAAGGTCTGTGGCCCCTCTCGCACCAACCCCAGCATGATGATTGATGACCTCC TGACTCCATGCTCACCAGGGGACCCAGGAGCCATGGAGATGACTTGGATGGATGTCCCTGGGGACAAACT CTTAGAGCCTGTGGTTTGCATGTCGGACATGCTGCGGTCTCTGGCCACCACCCGGCCCACGGTGAATGCA GACGACCTCCTGAAAGTGAAGAAATTCTCAGAGGACTTTGGGCAAGAGAGT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC208099 protein sequence
Red=Cloning site Green=Tags(s) MTTSTLQKAIDLVTKATEEDKAKNYEEALRLYQHAVEYFLHAIKYEAHSDKAKESIRAKCVQYLDRAEKL KDYLRSKEKHGKKPVKENQSEGKGSDSDSEGDNPEKKKLQEQLMGAVVMEKPNIRWNDVAGLEGAKEALK EAVILPIKFPHLFTGKRTPWRGILLFGPPGTGKSYLAKAVATEANNSTFFSVSSSDLMSKWLGESEKLVK NLFELARQHKPSIIFIDEVDSLCGSRNENESEAARRIKTEFLVQMQGVGNNNDGTLVLGATNIPWVLDSA IRRRFEKRIYIPLPEEAARAQMFRLHLGSTPHNLTDANIHELARKTEGYSGADISIIVRDSLMQPVRKVQ SATHFKKVCGPSRTNPSMMIDDLLTPCSPGDPGAMEMTWMDVPGDKLLEPVVCMSDMLRSLATTRPTVNA DDLLKVKKFSEDFGQES TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_013245 |
| ORF Size | 1311 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_013245.3 |
| RefSeq Size | 2211 bp |
| RefSeq ORF | 1314 bp |
| Locus ID | 27183 |
| UniProt ID | Q9UN37 |
| Domains | AAA, AAA, MIT |
| Protein Pathways | Endocytosis |
| MW | 48.9 kDa |
| Gene Summary | The protein encoded by this gene is a member of the AAA protein family (ATPases associated with diverse cellular activities), and is the homolog of the yeast Vps4 protein. In humans, two paralogs of the yeast protein have been identified. The former share a high degree of aa sequence similarity with each other, and also with yeast Vps4 and mouse Skd1 proteins. The mouse Skd1 (suppressor of K+ transport defect 1) has been shown to be really an yeast Vps4 ortholog. Functional studies indicate that both human paralogs associate with the endosomal compartments, and are involved in intracellular protein trafficking, similar to Vps4 protein in yeast. The gene encoding this paralog has been mapped to chromosome 16; the gene for the other resides on chromosome 18. [provided by RefSeq, Jul 2008] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC208099L1 | Lenti ORF clone of Human vacuolar protein sorting 4 homolog A (S. cerevisiae) (VPS4A), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC208099L2 | Lenti ORF clone of Human vacuolar protein sorting 4 homolog A (S. cerevisiae) (VPS4A), mGFP tagged |
CNY 5,890.00 |
|
| RC208099L3 | Lenti ORF clone of Human vacuolar protein sorting 4 homolog A (S. cerevisiae) (VPS4A), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC208099L4 | Lenti ORF clone of Human vacuolar protein sorting 4 homolog A (S. cerevisiae) (VPS4A), mGFP tagged |
CNY 5,890.00 |
|
| RG208099 | VPS4A (tGFP-tagged) - Human vacuolar protein sorting 4 homolog A (S. cerevisiae) (VPS4A) |
CNY 4,370.00 |
|
| SC115346 | VPS4A (untagged)-Human vacuolar protein sorting 4 homolog A (S. cerevisiae) (VPS4A) |
CNY 2,950.00 |
