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DDX1 (NM_004939) Human Tagged ORF Clone
CAT＃: RC208769
DDX1 (Myc-DDK-tagged)-Human DEAD (Asp-Glu-Ala-Asp) box polypeptide 1 (DDX1)
ORF Plasmid: tGFP
"NM_004939" in other vectors (6)
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CNY 3,990.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | DBP-RB; UKVH5d |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC208769 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGCCTTCTCCGAGATGGGTGTAATGCCTGAGATTGCACAAGCTGTGGAAGAGATGGATTGGCTCC TCCCAACTGATATCCAGGCTGAATCTATCCCATTGATCTTAGGAGGAGGTGATGTACTTATGGCTGCAGA AACAGGAAGTGGCAAAACTGGTGCTTTTAGTATTCCAGTTATCCAGATAGTTTATGAAACTCTGAAAGAC CAACAGGAAGGCAAAAAAGGAAAAACAACAATTAAAACTGGTGCTTCAGTGCTGAACAAATGGCAGATGA ACCCATATGACAGAGGATCTGCTTTTGCAATTGGGTCAGATGGTCTTTGTTGTCAAAGCAGAGAAGTAAA GGAATGGCATGGGTGTAGAGCTACTAAAGGATTAATGAAAGGGAAACACTACTATGAAGTATCCTGTCAT GACCAAGGGTTATGCAGGGTCGGGTGGTCTACCATGCAGGCCTCTTTGGACCTAGGTACTGACAAGTTTG GATTTGGCTTTGGTGGAACAGGAAAGAAATCCCATAACAAACAATTTGATAATTATGGAGAGGAATTCAC TATGCATGATACCATTGGATGTTACCTGGATATAGATAAGGGACATGTCAAGTTCTCCAAAAATGGAAAA GATCTTGGTCTGGCATTTGAAATACCACCACATATGAAAAACCAAGCCCTCTTTCCTGCCTGTGTTTTGA AGAATGCTGAACTGAAATTTAACTTCGGTGAAGAGGAATTTAAGTTTCCACCAAAAGATGGCTTTGTTGC TCTTTCCAAGGCACCGGATGGTTACATTGTCAAATCACAGCACTCAGGTAATGCACAGGTGACACAAACA AAGTTTCTCCCCAATGCTCCGAAAGCTCTCATTGTTGAACCTTCCCGGGAGTTAGCTGAACAAACTTTGA ACAACATCAAGCAGTTTAAGAAATACATTGATAATCCTAAATTAAGGGAGCTTCTGATAATTGGAGGTGT TGCAGCCCGGGATCAGCTCTCTGTTTTGGAAAATGGAGTAGATATAGTTGTAGGTACTCCGGGAAGACTA GATGACTTGGTGTCAACTGGAAAGCTGAACTTATCTCAAGTTAGATTCCTGGTCCTGGATGAAGCTGATG GGCTTCTTTCTCAAGGTTATTCTGATTTTATAAATAGGATGCACAATCAGATTCCTCAGGTTACCTCTGA TGGAAAAAGACTTCAGGTGATTGTTTGCTCTGCCACTTTGCATTCTTTCGATGTAAAGAAACTGTCCGAG AAGATAATGCATTTTCCTACATGGGTTGACTTAAAAGGAGAAGACTCTGTTCCAGATACTGTACACCATG TTGTTGTCCCAGTAAATCCCAAAACTGACAGACTCTGGGAAAGGCTTGGAAAGAGCCACATTAGAACTGA TGATGTACATGCAAAAGATAACACAAGACCTGGTGCTAATAGTCCAGAGATGTGGTCTGAAGCTATTAAA ATCCTGAAAGGGGAGTATGCTGTCCGGGCAATCAAGGAACATAAGATGGATCAAGCAATTATCTTCTGTA GAACCAAAATTGACTGTGATAACTTGGAGCAGTACTTTATACAACAAGGAGGAGGACCTGATAAAAAAGG ACACCAGTTCTCATGTGTTTGTCTTCATGGTGACAGAAAGCCTCATGAGAGAAAGCAAAACTTGGAAAGA TTTAAGAAAGGAGATGTAAGATTCTTGATTTGCACAGATGTAGCTGCTAGAGGAATTGATATCCACGGTG TTCCTTATGTTATAAATGTCACTCTGCCCGATGAAAAGCAAAACTACGTACATCGAATTGGCAGAGTAGG AAGAGCTGAAAGGATGGGTCTGGCAATTTCCCTGGTGGCAACAGAAAAAGAAAAGGTTTGGTACCATGTA TGTAGCAGCCGTGGAAAAGGGTGTTATAACACAAGACTCAAGGAAGATGGAGGCTGTACCATATGGTACA ACGAGATGCAGTTACTATCTGAGATAGAAGAACACCTGAACTGTACCATTTCTCAGGTTGAGCCGGATAT AAAGGTACCAGTGGATGAATTTGATGGGAAAGTTACCTACGGTCAGAAAAGGGCTGCTGGTGGTGGAAGC TATAAAGGCCATGTGGATATTTTGGCACCTACTGTTCAAGAGTTGGCTGCCCTTGAAAAGGAGGCGCAGA CATCTTTCCTGCATCTTGGCTACCTTCCTAACCAGCTGTTCAGAACCTTC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC208769 protein sequence
Red=Cloning site Green=Tags(s) MAAFSEMGVMPEIAQAVEEMDWLLPTDIQAESIPLILGGGDVLMAAETGSGKTGAFSIPVIQIVYETLKD QQEGKKGKTTIKTGASVLNKWQMNPYDRGSAFAIGSDGLCCQSREVKEWHGCRATKGLMKGKHYYEVSCH DQGLCRVGWSTMQASLDLGTDKFGFGFGGTGKKSHNKQFDNYGEEFTMHDTIGCYLDIDKGHVKFSKNGK DLGLAFEIPPHMKNQALFPACVLKNAELKFNFGEEEFKFPPKDGFVALSKAPDGYIVKSQHSGNAQVTQT KFLPNAPKALIVEPSRELAEQTLNNIKQFKKYIDNPKLRELLIIGGVAARDQLSVLENGVDIVVGTPGRL DDLVSTGKLNLSQVRFLVLDEADGLLSQGYSDFINRMHNQIPQVTSDGKRLQVIVCSATLHSFDVKKLSE KIMHFPTWVDLKGEDSVPDTVHHVVVPVNPKTDRLWERLGKSHIRTDDVHAKDNTRPGANSPEMWSEAIK ILKGEYAVRAIKEHKMDQAIIFCRTKIDCDNLEQYFIQQGGGPDKKGHQFSCVCLHGDRKPHERKQNLER FKKGDVRFLICTDVAARGIDIHGVPYVINVTLPDEKQNYVHRIGRVGRAERMGLAISLVATEKEKVWYHV CSSRGKGCYNTRLKEDGGCTIWYNEMQLLSEIEEHLNCTISQVEPDIKVPVDEFDGKVTYGQKRAAGGGS YKGHVDILAPTVQELAALEKEAQTSFLHLGYLPNQLFRTF TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_004939 |
| ORF Size | 2220 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_004939.3 |
| RefSeq Size | 2755 bp |
| RefSeq ORF | 2223 bp |
| Locus ID | 1653 |
| UniProt ID | Q92499 |
| Domains | DEAD, helicase_C, SPRY |
| Protein Families | Druggable Genome |
| MW | 82.4 kDa |
| Gene Summary | DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box protein of unknown function. It shows high transcription levels in 2 retinoblastoma cell lines and in tissues of neuroectodermal origin. [provided by RefSeq, Jul 2008] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC208769L1 | Lenti ORF clone of Human DEAD (Asp-Glu-Ala-Asp) box polypeptide 1 (DDX1), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC208769L2 | Lenti ORF clone of Human DEAD (Asp-Glu-Ala-Asp) box polypeptide 1 (DDX1), mGFP tagged |
CNY 5,890.00 |
|
| RC208769L3 | Lenti ORF clone of Human DEAD (Asp-Glu-Ala-Asp) box polypeptide 1 (DDX1), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC208769L4 | Lenti ORF clone of Human DEAD (Asp-Glu-Ala-Asp) box polypeptide 1 (DDX1), mGFP tagged |
CNY 5,890.00 |
|
| RG208769 | DDX1 (tGFP-tagged) - Human DEAD (Asp-Glu-Ala-Asp) box polypeptide 1 (DDX1) |
CNY 4,370.00 |
|
| SC117041 | DDX1 (untagged)-Human DEAD (Asp-Glu-Ala-Asp) box polypeptide 1 (DDX1) |
CNY 2,950.00 |
