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SRGAP1 (NM_020762) Human Tagged ORF Clone
CAT＃: RC208812
SRGAP1 (Myc-DDK-tagged)-Human SLIT-ROBO Rho GTPase activating protein 1 (SRGAP1)
ORF Plasmid: tGFP
"NM_020762" in other vectors (6)
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CNY 7,552.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | ARHGAP13; NMTC2 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC208812 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCCACCCCGAGCCGATTCAAGAAGGACAAAGAGATCATAGCCGAGTATGAAAGTCAAGTCAAAGAAA TTCGAGCTCAACTGGTAGAACAACAAAAATGCCTGGAGCAGCAAACGGAGATGCGAGTTCAGCTTCTCCA GGATCTGCAAGATTTCTTCCGAAAAAAAGCTGAAATTGAGACGGAATATTCCCGGAATCTAGAGAAGTTA GCAGAAAGGTTCATGGCAAAAACAAGAAGCACTAAGGATCATCAACAATACAAGAAAGACCAGAACCTGT TGTCTCCAGTGAACTGCTGGTATTTGCTCCTGAACCAAGTAAGGAGAGAAAGCAAAGACCATGCAACCTT GAGTGACATCTATCTGAACAATGTGATTATGCGGTTCATGCAGATAAGTGAGGATTCTACCAGGATGTTT AAAAAGAGCAAAGAGATTGCATTCCAACTTCATGAGGATTTAATGAAGGTTCTTAATGAGCTTTATACGG TGATGAAAACATACCATATGTATCATGCAGAGAGCATCAGTGCAGAGAGCAAGCTGAAAGAGGCCGAAAA ACAAGAGGAAAAGCAAATTGGGAGATCTGGTGATCCAGTCTTCCATATTCGACTAGAGGAGAGACATCAA CGGCGAAGCTCTGTAAAGAAAATTGAAAAAATGAAAGAAAAAAGACAAGCAAAATATTCAGAAAATAAGC TAAAATCAATTAAGGCACGGAACGAATATCTCCTAACACTTGAAGCCACCAATGCCTCAGTTTTCAAGTA CTATATTCATGATCTTTCTGATTTAATTGATTGCTGTGATCTTGGCTACCATGCAAGTCTGAACAGAGCC CTAAGAACATATCTGTCTGCGGAGTACAACCTTGAAACCTCCAGACATGAGGGCTTAGACATTATTGAGA ATGCAGTTGATAATTTAGAGCCCAGGAGCGATAAGCAGAGATTCATGGAGATGTACCCTGCTGCGTTCTG TCCACCAATGAAGTTTGAGTTTCAGTCTCACATGGGTGATGAGGTGTGCCAGGTCAGTGCCCAGCAGCCA GTCCAGGCAGAGCTCATGCTCAGGTACCAACAGTTGCAGTCCCGCCTTGCCACGCTCAAAATCGAGAATG AAGAGGTTAAGAAAACGACTGAAGCCACCTTGCAGACGATACAAGATATGGTCACCATCGAGGACTATGA TGTTTCTGAATGCTTCCAGCACAGTCGTTCCACAGAATCTGTGAAGTCCACTGTCTCTGAAACCTACCTG AGTAAACCCAGCATCGCCAAGAGAAGAGCCAACCAGCAGGAAACTGAACAGTTCTACTTCATGAAACTCA GAGAATATTTGGAAGGCAGTAATCTCATCACAAAACTTCAAGCCAAACATGACTTGCTGCAGAGGACCCT GGGAGAAGGTCATAGAGCTGAATATATGACTACAAGGCCTCCAAATGTTCCCCCTAAGCCCCAGAAACAC AGGAAGTCCAGGCCCCGCTCACAGTATAATACTAAGTTGTTTAATGGGGATTTGGAAACATTCGTCAAGG ACTCAGGACAGGTTATTCCCCTCATTGTGGAAAGCTGTATTCGGTTCATCAATCTCTATGGTCTTCAGCA TCAGGGGATTTTCAGAGTGTCTGGTTCCCAGGTGGAAGTCAATGATATTAAAAATTCATTTGAGAGAGGT GAAAATCCTTTGGCTGATGACCAGAGTAACCATGATATTAACTCAGTTGCTGGCGTTCTGAAGCTCTATT TCCGTGGGCTGGAAAACCCCCTCTTTCCTAAGGAAAGATTTAACGATCTGATTTCTTGTATCAGAATAGA TAATCTCTATGAGAGGGCGCTTCACATCCGCAAACTCCTCCTGACTTTGCCCAGGTCGGTCCTTATAGTG ATGAGGTACCTCTTTGCCTTCCTCAATCATCTATCACAGTACAGCGATGAGAATATGATGGACCCTTATA ACCTGGCCATTTGCTTTGGCCCAACATTGATGCCTGTCCCAGAAATACAGGATCAAGTGTCTTGCCAGGC ACATGTGAATGAAATTATCAAAACCATCATCATCCACCATGAGACTATTTTCCCAGATGCTAAAGAGCTG GATGGCCCTGTTTATGAGAAATGTATGGCTGGAGATGACTATTGCGACAGCCCATACAGTGAGCACGGTA CATTGGAGGAAGTGGACCAAGATGCTGGTACAGAGCCCCACACAAGTGAAGATGAATGTGAGCCAATAGA AGCAATAGCCAAGTTTGACTATGTTGGGCGGTCCGCCAGAGAACTATCCTTCAAGAAGGGTGCCTCCCTG CTGCTGTATCACCGTGCATCTGAGGACTGGTGGGAAGGCAGGCACAACGGGATTGACGGGCTGGTGCCTC ACCAGTATATAGTGGTGCAGGATATGGATGATACGTTTTCAGACACTCTGAGCCAAAAAGCCGACAGTGA GGCCAGCAGTGGGCCAGTCACGGAAGACAAGTCCTCATCCAAGGACATGAACTCCCCGACAGACCGTCAT CCTGACGGCTATTTAGCCAGGCAACGAAAAAGAGGAGAGCCACCCCCTCCAGTAAGGCGTCCTGGCAGGA CCAGTGATGGCCATTGCCCGCTCCACCCTCCACATGCCCTTTCTAACTCCTCAGTTGACCTAGGGTCCCC AAGCCTTGCCAGTCACCCCCGGGGCCTGCTGCAGAACCGTGGCCTCAACAATGACAGTCCTGAGCGGAGG CGCAGGCCTGGCCATGGCAGCCTGACCAACATCAGCCGGCACGACTCCCTCAAGAAGATCGACAGCCCTC CCATTAGAAGGTCCACGTCATCAGGGCAATACACGGGCTTCAATGACCACAAGCCACTGGACCCAGAGAC AATTGCTCAGGATATTGAAGAAACGATGAACACAGCTTTGAATGAACTCCGAGAACTGGAGAGACAGAGC ACAGCAAAGCATGCCCCTGATGTGGTGCTGGATACCCTGGAGCAAGTGAAAAACTCTCCCACCCCTGCCA CTTCCACGGAATCTCTCAGCCCTTTGCACAACGTTGCCCTCAGGAGCTCCGAGCCTCAGATTCGACGTAG CACGAGCTCCTCCAGTGACACAATGAGTACTTTCAAGCCTATGGTGGCACCCAGAATGGGCGTGCAGCTG AAGCCTCCAGCCCTTAGGCCAAAACCTGCTGTTCTTCCAAAAACAAATCCTACCATAGGACCTGCCCCAC CTCCCCAGGGTCCAACAGACAAGTCATGCACAATG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC208812 protein sequence
Red=Cloning site Green=Tags(s) MSTPSRFKKDKEIIAEYESQVKEIRAQLVEQQKCLEQQTEMRVQLLQDLQDFFRKKAEIETEYSRNLEKL AERFMAKTRSTKDHQQYKKDQNLLSPVNCWYLLLNQVRRESKDHATLSDIYLNNVIMRFMQISEDSTRMF KKSKEIAFQLHEDLMKVLNELYTVMKTYHMYHAESISAESKLKEAEKQEEKQIGRSGDPVFHIRLEERHQ RRSSVKKIEKMKEKRQAKYSENKLKSIKARNEYLLTLEATNASVFKYYIHDLSDLIDCCDLGYHASLNRA LRTYLSAEYNLETSRHEGLDIIENAVDNLEPRSDKQRFMEMYPAAFCPPMKFEFQSHMGDEVCQVSAQQP VQAELMLRYQQLQSRLATLKIENEEVKKTTEATLQTIQDMVTIEDYDVSECFQHSRSTESVKSTVSETYL SKPSIAKRRANQQETEQFYFMKLREYLEGSNLITKLQAKHDLLQRTLGEGHRAEYMTTRPPNVPPKPQKH RKSRPRSQYNTKLFNGDLETFVKDSGQVIPLIVESCIRFINLYGLQHQGIFRVSGSQVEVNDIKNSFERG ENPLADDQSNHDINSVAGVLKLYFRGLENPLFPKERFNDLISCIRIDNLYERALHIRKLLLTLPRSVLIV MRYLFAFLNHLSQYSDENMMDPYNLAICFGPTLMPVPEIQDQVSCQAHVNEIIKTIIIHHETIFPDAKEL DGPVYEKCMAGDDYCDSPYSEHGTLEEVDQDAGTEPHTSEDECEPIEAIAKFDYVGRSARELSFKKGASL LLYHRASEDWWEGRHNGIDGLVPHQYIVVQDMDDTFSDTLSQKADSEASSGPVTEDKSSSKDMNSPTDRH PDGYLARQRKRGEPPPPVRRPGRTSDGHCPLHPPHALSNSSVDLGSPSLASHPRGLLQNRGLNNDSPERR RRPGHGSLTNISRHDSLKKIDSPPIRRSTSSGQYTGFNDHKPLDPETIAQDIEETMNTALNELRELERQS TAKHAPDVVLDTLEQVKNSPTPATSTESLSPLHNVALRSSEPQIRRSTSSSSDTMSTFKPMVAPRMGVQL KPPALRPKPAVLPKTNPTIGPAPPPQGPTDKSCTM TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_020762 |
| ORF Size | 3255 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_020762.1, NP_065813.1 |
| RefSeq Size | 8475 bp |
| RefSeq ORF | 3258 bp |
| Locus ID | 57522 |
| UniProt ID | Q7Z6B7 |
| Protein Pathways | Axon guidance |
| MW | 124.3 kDa |
| Gene Summary | The protein encoded by this gene is a GTPase activator, working with the GTPase CDC42 to negatively regulate neuronal migration. The encoded protein interacts with the transmembrane receptor ROBO1 to inactivate CDC42. [provided by RefSeq, Sep 2016] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC208812L1 | Lenti ORF clone of Human SLIT-ROBO Rho GTPase activating protein 1 (SRGAP1), Myc-DDK-tagged |
CNY 9,952.00 |
|
| RC208812L2 | Lenti ORF clone of Human SLIT-ROBO Rho GTPase activating protein 1 (SRGAP1), mGFP tagged |
CNY 10,260.00 |
|
| RC208812L3 | Lenti ORF clone of Human SLIT-ROBO Rho GTPase activating protein 1 (SRGAP1), Myc-DDK-tagged |
CNY 10,260.00 |
|
| RC208812L4 | Lenti ORF clone of Human SLIT-ROBO Rho GTPase activating protein 1 (SRGAP1), mGFP tagged |
CNY 10,260.00 |
|
| RG208812 | SRGAP1 (tGFP-tagged) - Human SLIT-ROBO Rho GTPase activating protein 1 (SRGAP1) |
CNY 9,220.00 |
|
| SC304820 | SRGAP1 (untagged)-Human SLIT-ROBO Rho GTPase activating protein 1 (SRGAP1) |
CNY 7,560.00 |
