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TJAP1 (NM_080604) Human Tagged ORF Clone
CAT＃: RC209031
TJAP1 (Myc-DDK-tagged)-Human tight junction associated protein 1 (peripheral) (TJAP1), transcript variant 4
ORF Plasmid: tGFP
"NM_080604" in other vectors (4)
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CNY 3,990.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | PILT; TJP4 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC209031 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACTAGTGCCGCCCCTGCTAAGAAACCCTACCGTAAGGCACCACCAGAGCATCGGGAGCTGCGTTTGG AAATTCCTGGATCCCGGCTTGAGCAGGAGGAACCCCTGACTGATGCAGAAAGGATGAAGCTCTTACAGGA GGAGAATGAAGAGCTTCGCCGGCGCCTGGCCTCCGCCACCAGACGCACTGAGGCCCTGGAACGTGAGCTG GAAATTGGGCAGGACTGCCTGGAGCTGGAGCTGGGCCAGAGCCGCGAGGAGCTGGACAAATTTAAGGATA AGTTCCGCAGGCTGCAGAACAGCTACACGGCTTCCCAGCGGACCAACCAGGAGTTGGAGGACAAGCTGCA CACACTGATCAAGAAGGCTGAGATGGATAGGAAGACGCTGGACTGGGAGATTGTGGAGCTGACCAACAAG CTGCTGGATGCCAAGAACACCATCAACAAGCTGGAAGAGCTCAATGAGCGGTACCGGCTGGACTGCAACC TGGCTGTACAGCTCCTCAAGTGCAACAAGTCCCACTTCCGAAACCACAAGTTTGCCGATCTGCCCTGTGA GCTACAGGACATGGTTCGGAAACATTTGCACAGTGGTCAAGAGGCCGCCAGCCCAGGTCCTGCTCCCAGC CTAGCCCCAGGGGCTGTGGTGCCTACCTCAGTCATTGCCCGAGTGTTAGAGAAGCCGGAGTCTCTACTGC TCAATTCAGCCCAGTCAGGCAGCGCCGGGCGCCCCTTGGCTGAGGATGTCTTTGTGCATGTGGACATGAG TGAGGGTGTCCCAGGTGATCCAGCCAGTCCCCCGGCCCCTGGCAGCCCCACCCCACAACCCAATGGGGAG TGCCACTCTCTGGGTACTGCCAGGGGCTCCCCGGAGGAAGAGCTGCCCCTGCCAGCCTTTGAGAAGCTGA ACCCCTACCCAACCCCGTCTCCACCACACCCACTGTATCCTGGCCGCAGGGTAATAGAGTTCTCTGAGGA TAAGGTTCGGATCCCCCGCAACAGCCCCCTGCCCAACTGCACTTACGCTACCCGCCAGGCCATTTCCCTG AGCCTGGTAGAGGAGGGGAGTGAGCGGGCCCGCCCCAGCCCAGTGCCCAGCACCCCTGCCTCAGCCCAGG CCTCACCCCACCACCAGCCCAGCCCAGCACCCCTAACACTCAGTGCCCCAGCTAGCTCTGCCAGCTCTGA AGAGGACCTGCTGGTCAGCTGGCAGCGGGCATTTGTGGACCGTACTCCACCACCTGCTGCTGTGGCCCAG CGCACAGCCTTTGGACGCGATGCCCTCCCTGAGCTGCAGCGCCATTTTGCCCATAGCCCCGCTGACAGAG ATGAGGTGGTCCAGGCACCTTCTGCCCGACCCGAAGAGAGTGAGCTTTTGCTACCCACAGAACCTGACTC TGGCTTTCCCAGGGAGGAAGAAGAGCTGAACCTGCCTATCAGTCCTGAGGAAGAGCGCCAGAGCCTGCTG CCCATTAACAGGGGCACAGAGGAGGGGCCAGGCACTTCCCACACCGAGGGCAGGGCCTGGCCACTCCCCA GCTCCAGTCGCCCCCAGCGCAGCCCCAAGAGGATGGGGGTTCACCACCTGCACCGCAAGGACAGCCTGAC CCAGGCCCAGGAGCAGGGCAACCTGCTCAAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC209031 protein sequence
Red=Cloning site Green=Tags(s) MTSAAPAKKPYRKAPPEHRELRLEIPGSRLEQEEPLTDAERMKLLQEENEELRRRLASATRRTEALEREL EIGQDCLELELGQSREELDKFKDKFRRLQNSYTASQRTNQELEDKLHTLIKKAEMDRKTLDWEIVELTNK LLDAKNTINKLEELNERYRLDCNLAVQLLKCNKSHFRNHKFADLPCELQDMVRKHLHSGQEAASPGPAPS LAPGAVVPTSVIARVLEKPESLLLNSAQSGSAGRPLAEDVFVHVDMSEGVPGDPASPPAPGSPTPQPNGE CHSLGTARGSPEEELPLPAFEKLNPYPTPSPPHPLYPGRRVIEFSEDKVRIPRNSPLPNCTYATRQAISL SLVEEGSERARPSPVPSTPASAQASPHHQPSPAPLTLSAPASSASSEEDLLVSWQRAFVDRTPPPAAVAQ RTAFGRDALPELQRHFAHSPADRDEVVQAPSARPEESELLLPTEPDSGFPREEEELNLPISPEEERQSLL PINRGTEEGPGTSHTEGRAWPLPSSSRPQRSPKRMGVHHLHRKDSLTQAQEQGNLLN TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_080604 |
| ORF Size | 1641 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_080604.3 |
| RefSeq Size | 2783 bp |
| RefSeq ORF | 1644 bp |
| Locus ID | 93643 |
| UniProt ID | Q5JTD0 |
| Protein Pathways | Tight junction |
| MW | 60.7 kDa |
| Gene Summary | This gene encodes a tight junction-associated protein. Incorporation of the encoded protein into tight junctions occurs at a late stage of formation of the junctions. The encoded protein localizes to the Golgi and may function in vesicle trafficking. Alternatively spliced transcript variants have been described. A related pseudogene exists on the X chromosome. [provided by RefSeq, Mar 2009] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC209031L3 | Lenti ORF clone of Human tight junction associated protein 1 (peripheral) (TJAP1), transcript variant 4, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC209031L4 | Lenti ORF clone of Human tight junction associated protein 1 (peripheral) (TJAP1), transcript variant 4, mGFP tagged |
CNY 5,890.00 |
|
| RG209031 | TJAP1 (tGFP-tagged) - Human tight junction associated protein 1 (peripheral) (TJAP1), transcript variant 4 |
CNY 4,370.00 |
|
| SC112772 | TJAP1 (untagged)-Human tight junction associated protein 1 (peripheral) (TJAP1), transcript variant 4 |
CNY 2,950.00 |
