IPMK (NM_152230) Human Tagged ORF Clone

CAT#: RC209343

IPMK (Myc-DDK-tagged)-Human inositol polyphosphate multikinase (IPMK)

ORF Plasmid: DDK tGFP



  "NM_152230" in other vectors (6)

Need custom modification / cloning service?
Get a free quote

CNY 5,488.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (4)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

CNY 300.00


Rabbit Polyclonal antibody to IPMK (inositol polyphosphate multikinase)
    • 100 ul

CNY 6,281.00

Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC209343 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCAACAGAGCCACCATCCCCCCTCCGGGTCGAGGCGCCGGGCCCCCCAGAAATGCGGACCTCACCGG
CGATCGAGTCCACCCCTGAGGGCACCCCGCAGCCGGCGGGCGGCAGACTCCGCTTCCTCAACGGCTGCGT
GCCCCTCTCGCATCAGGTGGCCGGGCACATGTACGGGAAGGACAAAGTGGGTATACTGCAACATCCAGAT
GGCACAGTTTTGAAACAGTTACAACCACCTCCAAGGGGCCCAAGAGAGCTGGAATTCTATAATATGGTTT
ATGCTGCTGACTGTTTTGATGGTGTTCTTCTAGAGCTACGAAAATATTTGCCAAAATATTATGGCATCTG
GTCACCTCCCACTGCACCAAACGATTTATACCTAAAACTGGAAGATGTGACCCATAAATTTAATAAGCCC
TGTATAATGGATGTAAAGATAGGGCAAAAAAGCTATGATCCTTTTGCCTCATCTGAGAAGATTCAGCAAC
AGGTCAGCAAGTACCCATTAATGGAAGAGATTGGGTTCTTGGTGCTTGGCATGAGGGTTTATCATGTTCA
TTCCGATAGCTATGAGACAGAAAACCAGCATTACGGAAGAAGCTTAACAAAAGAAACTATAAAGGATGGA
GTCTCCAGATTTTTTCATAATGGGTACTGCTTAAGAAAAGATGCTGTTGCTGCCAGTATTCAGAAGATTG
AGAAAATTCTGCAGTGGTTTGAAAACCAGAAGCAGCTTAATTTTTACGCAAGTTCATTACTCTTTGTTTA
TGAAGGTTCATCTCAGCCAACCACTACAAAATTGAATGACAGAACTTTGGCAGAAAAGTTTTTGTCCAAA
GGACAACTGTCAGACACAGAAGTACTAGAGTACAATAATAACTTTCATGTGTTAAGTTCCACAGCTAATG
GAAAAATAGAGTCTTCAGTGGGCAAAAGCTTGTCCAAGATGTATGCGCGTCACAGGAAAATATATACAAA
AAAGCATCACAGTCAGACTTCATTGAAAGTTGAAAATCTGGAGCAAGACAATGGGTGGAAAAGCATGTCA
CAGGAACATTTAAATGGAAATGTACTTTCCCAACTGGAAAAAGTTTTCTACCATCTTCCCACTGGTTGCC
AAGAGATTGCTGAAGTAGAAGTGCGAATGATAGATTTTGCTCATGTGTTCCCTAGCAACACAATAGATGA
GGGATATGTTTATGGGCTAAAGCATTTAATTTCTGTACTTCGAAGTATTTTAGACAAT


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC209343 protein sequence
Red=Cloning site Green=Tags(s)

MATEPPSPLRVEAPGPPEMRTSPAIESTPEGTPQPAGGRLRFLNGCVPLSHQVAGHMYGKDKVGILQHPD
GTVLKQLQPPPRGPRELEFYNMVYAADCFDGVLLELRKYLPKYYGIWSPPTAPNDLYLKLEDVTHKFNKP
CIMDVKIGQKSYDPFASSEKIQQQVSKYPLMEEIGFLVLGMRVYHVHSDSYETENQHYGRSLTKETIKDG
VSRFFHNGYCLRKDAVAASIQKIEKILQWFENQKQLNFYASSLLFVYEGSSQPTTTKLNDRTLAEKFLSK
GQLSDTEVLEYNNNFHVLSSTANGKIESSVGKSLSKMYARHRKIYTKKHHSQTSLKVENLEQDNGWKSMS
QEHLNGNVLSQLEKVFYHLPTGCQEIAEVEVRMIDFAHVFPSNTIDEGYVYGLKHLISVLRSILDN

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_152230
ORF Size 1248 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_152230.5
RefSeq Size 6133 bp
RefSeq ORF 1251 bp
Locus ID 253430
UniProt ID Q8NFU5
Protein Pathways Inositol phosphate metabolism
MW 47.2 kDa
Gene Summary This gene encodes a member of the inositol phosphokinase family. The encoded protein has 3-kinase, 5-kinase and 6-kinase activities on phosphorylated inositol substrates. The encoded protein plays an important role in the biosynthesis of inositol 1,3,4,5,6-pentakisphosphate, and has a preferred 5-kinase activity. This gene may play a role in nuclear mRNA export. Pseudogenes of this gene are located on the long arm of chromosome 13 and the short arm of chromosome 19. [provided by RefSeq, Dec 2010]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Other Versions

Customer Reviews 
Loading...