ZNF313 (RNF114) (NM_018683) Human Tagged ORF Clone
CAT#: RC209752
RNF114 (Myc-DDK-tagged)-Human ring finger protein 114 (RNF114)
ORF Plasmid: tGFP
"NM_018683" in other vectors (6)
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CNY 2,400.00
CNY 3,705.00
Cited in 3 publications. |
CNY 300.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | PSORS12; ZNF313 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC209752 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGCGCAACAGCGGGACTGCGGGGGTGCTGCGCAGCTGGCGGGGCCGGCGGCGGAGGCTGACCCCC TAGGACGCTTCACGTGTCCCGTGTGCTTAGAGGTGTACGAGAAGCCGGTACAGGTGCCCTGCGGACACGT CTTTTGCTCTGCATGCCTGCAGGAATGTCTGAAGCCGAAGAAGCCTGTCTGTGGGGTGTGTCGCAGCGCT CTGGCACCTGGCGTCCGAGCCGTGGAGCTCGAGCGGCAGATCGAGAGCACAGAGACTTCTTGCCATGGCT GCCGTAAGAATTTCTTCCTGTCCAAGATCCGGTCCCACGTGGCTACTTGTTCCAAATACCAGAATTACAT CATGGAAGGTGTGAAGGCCACCATTAAGGATGCATCTCTTCAGCCAAGGAATGTTCCAAACCGTTACACC TTTCCTTGTCCTTACTGTCCTGAGAAGAACTTTGATCAGGAAGGACTTGTGGAACACTGCAAATTATTCC ATAGCACGGATACCAAATCTGTGGTTTGTCCGATATGTGCCTCGATGCCCTGGGGAGACCCCAACTACCG CAGCGCCAACTTCAGAGAGCACATCCAGCGCCGGCACCGGTTTTCTTATGACACTTTTGTGGATTATGAT GTTGATGAAGAGGACATGATGAATCAGGTGTTGCAGCGCTCCATCATCGACCAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC209752 protein sequence
Red=Cloning site Green=Tags(s) MAAQQRDCGGAAQLAGPAAEADPLGRFTCPVCLEVYEKPVQVPCGHVFCSACLQECLKPKKPVCGVCRSA LAPGVRAVELERQIESTETSCHGCRKNFFLSKIRSHVATCSKYQNYIMEGVKATIKDASLQPRNVPNRYT FPCPYCPEKNFDQEGLVEHCKLFHSTDTKSVVCPICASMPWGDPNYRSANFREHIQRRHRFSYDTFVDYD VDEEDMMNQVLQRSIIDQ TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
Sequencher program is needed, download here. |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_018683 |
ORF Size | 684 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_018683.4 |
RefSeq Size | 2478 bp |
RefSeq ORF | 687 bp |
Locus ID | 55905 |
UniProt ID | Q9Y508 |
Domains | RING, zf-C2H2 |
MW | 25.7 kDa |
Gene Summary | E3 ubiquitin-protein ligase promoting the ubiquitination and degradation of the CDK inhibitor CDKN1A and probably also CDKN1B and CDKN1C. These activities stimulate cell cycle's G1-to-S phase transition and suppress cellular senescence. May play a role in spermatogenesis.[UniProtKB/Swiss-Prot Function] |
Citations (3)
The use of this cDNA Clones has been cited in the following citations: |
---|
Harnessing the Anti-Cancer Natural Product Nimbolide for Targeted Protein Degradation
,null,
Nature chemical biology
,PubMed ID 31209351
[ZNF313]
|
Harnessing the Anti-Cancer Natural Product Nimbolide for Targeted Protein Degradation
,Spradlin, J;Hu, X;Ward, C;Brittain, S;Ou, L;Bussiere, D;Thomas, J;Tallarico, J;McKenna, J;Schirle, M;Maimone, T;Nomura, D;,
bioRxiv
[ZNF313]
|
The E3 ubiquitin ligase RNF114 and TAB1 degradation are required for maternal-to-zygotic transition
,Yang, Y;Zhou, C;Wang, Y;Liu, W;Liu, C;Wang, L;Liu, Y;Shang, Y;Li, M;Zhou, S;Wang, Y;Zeng, W;Zhou, J;Huo, R;Li, W;,
EMBO Rep
,PubMed ID 28073917
[ZNF313]
|
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC209752L1 | Lenti ORF clone of Human ring finger protein 114 (RNF114), Myc-DDK-tagged |
CNY 4,800.00 |
|
RC209752L2 | Lenti ORF clone of Human ring finger protein 114 (RNF114), mGFP tagged |
CNY 5,890.00 |
|
RC209752L3 | Lenti ORF clone of Human ring finger protein 114 (RNF114), Myc-DDK-tagged |
CNY 5,890.00 |
|
RC209752L4 | Lenti ORF clone of Human ring finger protein 114 (RNF114), mGFP tagged |
CNY 5,890.00 |
|
RG209752 | RNF114 (tGFP-tagged) - Human ring finger protein 114 (RNF114) |
CNY 4,370.00 |
|
SC113359 | RNF114 (untagged)-Human ring finger protein 114 (RNF114) |
CNY 2,400.00 |