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alpha 5 Defensin (DEFA5) (NM_021010) Human Tagged ORF Clone
CAT＃: RC210219
DEFA5 (Myc-DDK-tagged)-Human defensin, alpha 5, Paneth cell-specific (DEFA5)
ORF Plasmid: tGFP
"NM_021010" in other vectors (6)
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CNY 1,200.00
CNY 3,705.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | DEF5; HD-5 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC210219 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGGACCATCGCCATCCTTGCTGCCATTCTCCTGGTGGCCCTGCAGGCCCAGGCTGAGTCACTCCAGG AAAGAGCTGATGAGGCTACAACCCAGAAGCAGTCTGGGGAAGACAACCAGGACCTTGCTATCTCCTTTGC AGGAAATGGACTCTCTGCTCTTAGAACCTCAGGTTCTCAGGCAAGAGCCACCTGCTATTGCCGAACTGGC CGTTGTGCTACCCGTGAGTCCCTCTCCGGGGTGTGTGAAATCAGTGGCCGCCTCTACAGACTCTGCTGTC GC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC210219 protein sequence
Red=Cloning site Green=Tags(s) MRTIAILAAILLVALQAQAESLQERADEATTQKQSGEDNQDLAISFAGNGLSALRTSGSQARATCYCRTG RCATRESLSGVCEISGRLYRLCCR TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_021010 |
| ORF Size | 282 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_021010.3 |
| RefSeq Size | 468 bp |
| RefSeq ORF | 285 bp |
| Locus ID | 1670 |
| UniProt ID | Q01523 |
| Protein Families | Secreted Protein |
| MW | 10.1 kDa |
| Gene Summary | Defensins are a family of antimicrobial and cytotoxic peptides thought to be involved in host defense. They are abundant in the granules of neutrophils and also found in the epithelia of mucosal surfaces such as those of the intestine, respiratory tract, urinary tract, and vagina. Members of the defensin family are highly similar in protein sequence and distinguished by a conserved cysteine motif. Several of the alpha defensin genes appear to be clustered on chromosome 8. The protein encoded by this gene, defensin, alpha 5, is highly expressed in the secretory granules of Paneth cells of the ileum. [provided by RefSeq, Oct 2014] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC210219L1 | Lenti ORF clone of Human defensin, alpha 5, Paneth cell-specific (DEFA5), Myc-DDK-tagged |
CNY 3,600.00 |
|
| RC210219L2 | Lenti ORF clone of Human defensin, alpha 5, Paneth cell-specific (DEFA5), mGFP tagged |
CNY 5,890.00 |
|
| RC210219L3 | Lenti ORF clone of Human defensin, alpha 5, Paneth cell-specific (DEFA5), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC210219L4 | Lenti ORF clone of Human defensin, alpha 5, Paneth cell-specific (DEFA5), mGFP tagged |
CNY 5,890.00 |
|
| RG210219 | DEFA5 (tGFP-tagged) - Human defensin, alpha 5, Paneth cell-specific (DEFA5) |
CNY 2,800.00 |
|
| SC304891 | DEFA5 (untagged)-Human defensin, alpha 5, Paneth cell-specific (DEFA5) |
CNY 1,200.00 |
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