USP21 (NM_012475) Human Tagged ORF Clone

CAT#: RC210554

USP21 (Myc-DDK-tagged)-Human ubiquitin specific peptidase 21 (USP21), transcript variant 1

ORF Plasmid: DDK tGFP



  "NM_012475" in other vectors (6)

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CNY 3,990.00


货期*
现货

规格
    • 10 ug

Cited in 1 publication.

Product images

经常一起买 (5)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

CNY 300.00


USP21 rabbit polyclonal antibody
    • 100 ul

CNY 1,999.00
CNY 3,280.00

Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Synonyms USP16; USP23
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC210554 representing NM_012475
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCCCCAGGCCTCTGAGCACCGCCTGGGCCGTACCCGAGAGCCACCTGTTAATATCCAGCCCCGAGTGG
GATCCAAGCTACCATTTGCCCCCAGGGCCCGCAGCAAGGAGCGCAGAAACCCAGGCTCTGGGCCAAACCC
CATGTTACGACCTCTGCCTCCCCGGCCAGGTCTGCCTGATGAACGGCTCAAGAAACTGGAGCTGGGACGG
GGACGGACCTCAGGCCCTCGTCCCAGAGGCCCCCTTCGAGCAGATCATGGGGTTCCCCTGCCTGGCTCAC
CACCCCCAACAGTGGCTTTGCCTCTCCCATCTCGGACCAACTTAGCCCGTTCCAAGTCTGTGAGCAGTGG
GGACTTGCGTCCAATGGGGATTGCCTTGGGAGGGCACCGTGGCACCGGAGAGCTTGGGGCTGCACTGAGC
CGCTTGGCCCTCCGGCCTGAGCCACCCACTTTGAGACGTAGCACTTCTCTCCGCCGCCTAGGGGGCTTTC
CTGGACCCCCTACCCTGTTCAGCATACGGACAGAGCCCCCTGCTTCCCATGGCTCCTTCCACATGATATC
CGCCCGGTCCTCTGAGCCTTTCTACTCTGATGACAAGATGGCTCATCACACACTCCTTCTGGGCTCTGGT
CATGTTGGCCTTCGAAACCTGGGAAACACGTGCTTCCTGAATGCTGTGCTGCAGTGTCTGAGCAGCACTC
GACCTCTTCGGGACTTCTGTCTGAGAAGGGACTTCCGGCAAGAGGTGCCTGGAGGAGGCCGAGCCCAAGA
GCTCACTGAAGCCTTTGCAGATGTGATTGGTGCCCTCTGGCACCCTGACTCCTGCGAAGCTGTGAATCCT
ACTCGATTCCGAGCTGTCTTCCAGAAATATGTTCCCTCCTTCTCTGGATACAGCCAGCAGGATGCCCAAG
AGTTCCTGAAGCTCCTCATGGAGCGGCTACACCTTGAAATCAACCGCCGAGGCCGCCGGGCTCCACCGAT
ACTTGCCAATGGTCCAGTTCCCTCTCCACCCCGCCGAGGAGGGGCTCTGCTAGAAGAACCTGAGTTAAGT
GATGATGACCGAGCCAACCTAATGTGGAAACGTTACCTGGAGCGAGAGGACAGCAAGATTGTGGACCTGT
TTGTGGGCCAGTTGAAAAGTTGTCTCAAGTGCCAGGCCTGTGGGTATCGCTCCACGACCTTCGAGGTTTT
TTGTGACCTGTCCCTGCCCATCCCCAAGAAAGGATTTGCTGGGGGCAAGGTGTCTCTGCGGGATTGTTTC
AACCTTTTCACTAAGGAAGAAGAGCTAGAGTCGGAGAATGCCCCAGTGTGTGACCGATGTCGGCAGAAAA
CTCGAAGTACCAAAAAGTTGACAGTACAAAGATTCCCTCGAATCCTCGTGCTCCATCTGAATCGATTTTC
TGCCTCCCGAGGCTCCATCAAAAAAAGTTCAGTAGGTGTAGACTTTCCACTGCAGCGACTGAGCCTAGGG
GACTTTGCCAGTGACAAAGCCGGAAGTCCTGTATACCAGCTGTATGCCCTTTGCAACCACTCAGGCAGCG
TCCACTATGGCCACTACACAGCCCTGTGCCGGTGCCAGACTGGTTGGCATGTCTACAATGACTCTCGTGT
CTCCCCTGTCAGTGAAAACCAGGTGGCATCCAGCGAGGGCTACGTGCTGTTCTACCAACTGATGCAGGAG
CCACCCCGGTGCCTG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC210554 representing NM_012475
Red=Cloning site Green=Tags(s)

MPQASEHRLGRTREPPVNIQPRVGSKLPFAPRARSKERRNPGSGPNPMLRPLPPRPGLPDERLKKLELGR
GRTSGPRPRGPLRADHGVPLPGSPPPTVALPLPSRTNLARSKSVSSGDLRPMGIALGGHRGTGELGAALS
RLALRPEPPTLRRSTSLRRLGGFPGPPTLFSIRTEPPASHGSFHMISARSSEPFYSDDKMAHHTLLLGSG
HVGLRNLGNTCFLNAVLQCLSSTRPLRDFCLRRDFRQEVPGGGRAQELTEAFADVIGALWHPDSCEAVNP
TRFRAVFQKYVPSFSGYSQQDAQEFLKLLMERLHLEINRRGRRAPPILANGPVPSPPRRGGALLEEPELS
DDDRANLMWKRYLEREDSKIVDLFVGQLKSCLKCQACGYRSTTFEVFCDLSLPIPKKGFAGGKVSLRDCF
NLFTKEEELESENAPVCDRCRQKTRSTKKLTVQRFPRILVLHLNRFSASRGSIKKSSVGVDFPLQRLSLG
DFASDKAGSPVYQLYALCNHSGSVHYGHYTALCRCQTGWHVYNDSRVSPVSENQVASSEGYVLFYQLMQE
PPRCL

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_012475
ORF Size 1695 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_012475.5
RefSeq Size 2217 bp
RefSeq ORF 1698 bp
Locus ID 27005
UniProt ID Q9UK80
Domains UCH
Protein Families Druggable Genome, Protease
MW 62.5 kDa
Gene Summary This gene encodes a member of the C19 peptidase family, also known as family 2 of ubiquitin carboxy-terminal hydrolases. The encoded protein cleaves ubiquitin from ubiquitinated proteins for recycling in intracellular protein degradation. The encoded protein is also able to release NEDD8, a ubiquitin-like protein, from NEDD8-conjugated proteins. This gene has been referred to as USP16 and USP23 but is now known as USP21. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Feb 2016]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Citations (1)

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