United States
Germany
Japan
United Kingdom
China
NIT2 (NM_020202) Human Tagged ORF Clone
CAT＃: RC210660
NIT2 (Myc-DDK-tagged)-Human nitrilase family, member 2 (NIT2)
ORF Plasmid: tGFP
"NM_020202" in other vectors (6)
Need custom modification / cloning service?
Get a free quote
CNY 2,400.00
CNY 3,990.00
Product images
CNY 300.00
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | HEL-S-8a |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC210660 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACCTCTTTCCGCTTGGCCCTCATCCAGCTTCAGATTTCTTCCATCAAATCAGATAACGTCACTCGCG CTTGTAGCTTCATCCGGGAGGCAGCAACGCAAGGAGCCAAAATAGTTTCTTTGCCGGAATGCTTTAATTC TCCATATGGAGCGAAATATTTTCCTGAATATGCAGAGAAAATTCCTGGTGAATCCACACAGAAGCTTTCT GAAGTAGCAAAGGAATGCAGCATATATCTCATTGGAGGCTCTATCCCTGAAGAGGATGCTGGGAAATTAT ATAACACCTGTGCTGTGTTTGGGCCTGATGGAACTTTACTAGCAAAGTATAGAAAGATCCATCTGTTTGA CATTGATGTTCCTGGAAAAATTACATTTCAAGAATCTAAAACATTGAGTCCGGGTGATAGTTTCTCCACA TTTGATACTCCTTACTGCAGAGTGGGTCTGGGCATCTGCTACGACATGCGGTTTGCAGAGCTTGCACAAA TCTACGCACAGAGAGGCTGCCAGCTGTTGGTATATCCAGGAGCTTTTAATCTGACCACTGGACCAGCCCA TTGGGAGTTACTTCAGCGAAGCCGGGCTGTTGATAATCAGGTGTATGTGGCCACAGCCTCTCCTGCCCGG GATGACAAAGCCTCCTATGTTGCCTGGGGACACAGCACCGTGGTGAACCCTTGGGGGGAGGTTCTAGCCA AAGCTGGCACAGAAGAAGCAATCGTGTATTCAGACATAGACCTGAAGAAGCTGGCTGAAATACGCCAGCA AATCCCCGTTTTTAGACAGAAGCGATCAGACCTCTATGCTGTGGAGATGAAAAAGCCC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC210660 protein sequence
Red=Cloning site Green=Tags(s) MTSFRLALIQLQISSIKSDNVTRACSFIREAATQGAKIVSLPECFNSPYGAKYFPEYAEKIPGESTQKLS EVAKECSIYLIGGSIPEEDAGKLYNTCAVFGPDGTLLAKYRKIHLFDIDVPGKITFQESKTLSPGDSFST FDTPYCRVGLGICYDMRFAELAQIYAQRGCQLLVYPGAFNLTTGPAHWELLQRSRAVDNQVYVATASPAR DDKASYVAWGHSTVVNPWGEVLAKAGTEEAIVYSDIDLKKLAEIRQQIPVFRQKRSDLYAVEMKKP TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_020202 |
| ORF Size | 828 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_020202.5 |
| RefSeq Size | 1271 bp |
| RefSeq ORF | 831 bp |
| Locus ID | 56954 |
| UniProt ID | Q9NQR4 |
| Domains | CN_hydrolase |
| MW | 30.6 kDa |
| Gene Summary | Has a omega-amidase activity. The role of omega-amidase is to remove potentially toxic intermediates by converting alpha-ketoglutaramate and alpha-ketosuccinamate to biologically useful alpha-ketoglutarate and oxaloacetate, respectively. Overexpression decreases the colony-forming capacity of cultured cells by arresting cells in the G2 phase of the cell cycle.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC210660L1 | Lenti ORF clone of Human nitrilase family, member 2 (NIT2), Myc-DDK-tagged |
CNY 4,800.00 |
|
| RC210660L2 | Lenti ORF clone of Human nitrilase family, member 2 (NIT2), mGFP tagged |
CNY 5,890.00 |
|
| RC210660L3 | Lenti ORF clone of Human nitrilase family, member 2 (NIT2), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC210660L4 | Lenti ORF clone of Human nitrilase family, member 2 (NIT2), mGFP tagged |
CNY 5,890.00 |
|
| RG210660 | NIT2 (tGFP-tagged) - Human nitrilase family, member 2 (NIT2) |
CNY 4,000.00 |
|
| SC108295 | NIT2 (untagged)-Human nitrilase family, member 2 (NIT2) |
CNY 2,400.00 |
