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Caldesmon (CALD1) (NM_033139) Human Tagged ORF Clone
CAT＃: RC214238
- TrueORF®
CALD1 (Myc-DDK-tagged)-Human caldesmon 1 (CALD1), transcript variant 4
ORF Plasmid: tGFP
"NM_033139" in other vectors (6)
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CNY 6,232.00
Product images
CNY 300.00
CNY 1,999.00
CNY 3,600.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | CDM; H-CAD; HCAD; L-CAD; LCAD; NAG22 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC214238 representing NM_033139
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCTGGGTGGATCCGGATCGCATGGAAGACGCAGCCTGGCCGCGCTCTCCCAAATCGCCTACCAGAGGA ATGACGATGATGAAGAGGAGGCAGCCCGGGAACGGCGCCGCCGAGCCCGACAGGAACGGCTGCGGCAGAA GCAGGAGGAAGAATCCTTGGGACAGGTGACCGACCAGGTGGAGGTGAATGCCCAGAACAGTGTGCCTGAC GAGGAGGCCAAGACAACCACCACAAACACTCAAGTGGAAGGGGATGATGAGGCCGCATTCCTGGAGCGCC TGGCTCGGCGTGAGGAAAGACGCCAAAAACGCCTTCAGGAGGCTCTGGAGCGGCAGAAGGAGTTCGACCC AACAATAACAGATGCAAGTCTGTCGCTCCCAAGCAGAAGAATGCAAAATGACACAGCAGAAAATGAAACT ACCGAGAAGGAAGAAAAAAGTGAAAGTCGCCAAGAAAGATACGAGATAGAGGAAACAGAAACAGTCACCA AGTCCTACCAGAAGAATGATTGGAGGGATGCTGAAGAAAACAAGAAAGAAGACAAGGAAAAGGAGGAGGA GGAAGAGGAGAAGCCAAAGCGAGGGAGCATTGGAGAAAATCAGGGAGAAGAGAAGGGAACTAAAGTGCAA GCTAAAAGAGAAAAGCTCCAAGAAGACAAGCCTACCTTCAAAAAAGAAGAGATCAAAGATGAAAAGATTA AAAAGGACAAAGAACCCAAAGAAGAAGTTAAGAGCTTCATGGATCGAAAGAAGGGATTTACAGAAGTTAA GTCGCAGAATGGAGAATTCATGACCCACAAACTTAAACATACTGAGAATACTTTCAGCCGCCCTGGAGGG AGGGCCAGCGTGGACACCAAGGAGGCTGAGGGCGCCCCCCAGGTGGAAGCCGGCAAAAGGCTGGAGGAGC TTCGTCGTCGTCGCGGGGAGACCGAGAGCGAAGAGTTCGAGAAGCTCAAACAGAAGCAGCAGGAGGCGGC TTTGGAGCTGGAGGAACTCAAGAAAAAGAGGGAGGAGAGAAGGAAGGTCCTGGAGGAGGAAGAGCAGAGG AGGAAGCAGGAGGAAGCCGATCGAAAACTCAGAGAGGAGGAAGAGAAGAGGAGGCTAAAGGAAGAGATTG AAAGGCGAAGAGCAGAAGCTGCTGAGAAACGCCAGAAGATGCCAGAAGATGGCTTGTCAGATGACAAGAA ACCATTCAAGTGTTTCACTCCTAAAGGTTCATCTCTCAAGATAGAAGAGCGAGCAGAATTTTTGAATAAG TCTGTGCAGAAAAGCAGTGGTGTCAAATCGACCCATCAAGCAGCAATAGTCTCCAAGATTGACAGCAGAC TGGAGCAGTATACCAGTGCAATTGAGGGAACAAAAAGCGCAAAACCTACAAAGCCGGCAGCCTCGGATCT TCCTGTTCCTGCTGAAGGTGTACGCAACATCAAGAGTATGTGGGAGAAAGGGAATGTGTTTTCATCCCCC ACTGCAGCAGGCACACCAAATAAGGAAACTGCTGGCTTGAAGGTAGGGGTTTCTAGCCGCATCAATGAAT GGCTAACTAAAACCCCAGATGGAAACAAGTCACCTGCTCCCAAACCTTCTGACTTGAGACCAGGAGACGT ATCCAGCAAGCGGAACCTCTGGGAAAAGCAATCTGTGGATAAGGTCACTTCCCCCACTAAGGTT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC214238 representing NM_033139
Red=Cloning site Green=Tags(s) MLGGSGSHGRRSLAALSQIAYQRNDDDEEEAARERRRRARQERLRQKQEEESLGQVTDQVEVNAQNSVPD EEAKTTTTNTQVEGDDEAAFLERLARREERRQKRLQEALERQKEFDPTITDASLSLPSRRMQNDTAENET TEKEEKSESRQERYEIEETETVTKSYQKNDWRDAEENKKEDKEKEEEEEEKPKRGSIGENQGEEKGTKVQ AKREKLQEDKPTFKKEEIKDEKIKKDKEPKEEVKSFMDRKKGFTEVKSQNGEFMTHKLKHTENTFSRPGG RASVDTKEAEGAPQVEAGKRLEELRRRRGETESEEFEKLKQKQQEAALELEELKKKREERRKVLEEEEQR RKQEEADRKLREEEEKRRLKEEIERRRAEAAEKRQKMPEDGLSDDKKPFKCFTPKGSSLKIEERAEFLNK SVQKSSGVKSTHQAAIVSKIDSRLEQYTSAIEGTKSAKPTKPAASDLPVPAEGVRNIKSMWEKGNVFSSP TAAGTPNKETAGLKVGVSSRINEWLTKTPDGNKSPAPKPSDLRPGDVSSKRNLWEKQSVDKVTSPTKV TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_033139 |
| ORF Size | 1674 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_033139.3, NP_149130.1 |
| RefSeq Size | 4282 bp |
| RefSeq ORF | 1677 bp |
| Locus ID | 800 |
| UniProt ID | Q05682 |
| Domains | Caldesmon |
| Protein Pathways | Vascular smooth muscle contraction |
| MW | 64.1 kDa |
| Gene Summary | This gene encodes a calmodulin- and actin-binding protein that plays an essential role in the regulation of smooth muscle and nonmuscle contraction. The conserved domain of this protein possesses the binding activities to Ca(2+)-calmodulin, actin, tropomyosin, myosin, and phospholipids. This protein is a potent inhibitor of the actin-tropomyosin activated myosin MgATPase, and serves as a mediating factor for Ca(2+)-dependent inhibition of smooth muscle contraction. Alternative splicing of this gene results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Jul 2008] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC214238L1 | Lenti-ORF clone of CALD1 (Myc-DDK-tagged)-Human caldesmon 1 (CALD1), transcript variant 4 |
CNY 8,632.00 |
|
| RC214238L2 | Lenti-ORF clone of CALD1 (mGFP-tagged)-Human caldesmon 1 (CALD1), transcript variant 4 |
CNY 6,460.00 |
|
| RC214238L3 | Lenti-ORF clone of CALD1 (Myc-DDK-tagged)-Human caldesmon 1 (CALD1), transcript variant 4 |
CNY 6,460.00 |
|
| RC214238L4 | Lenti-ORF clone of CALD1 (mGFP-tagged)-Human caldesmon 1 (CALD1), transcript variant 4 |
CNY 6,460.00 |
|
| RG214238 | CALD1 (tGFP-tagged) - Human caldesmon 1 (CALD1), transcript variant 4 |
CNY 7,832.00 |
|
| SC127344 | CALD1 (untagged)-Human caldesmon 1 (CALD1), transcript variant 4 |
CNY 6,240.00 |
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