HCFC1 (NM_005334) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	CFF; HCF; HCF-1; HCF1; HFC1; MRX3; PPP1R89; VCAF
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC215341 representing NM_005334 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTTCGGCCGTGTCGCCCGCCAACTTGCCAGCGGTGCTTCTGCAGCCCCGCTGGAAGCGAGTGGTGG GCTGGTCGGGTCCGGTGCCACGGCCCCGCCACGGCCACCGCGCCGTGGCCATCAAGGAGCTCATCGTGGT GTTTGGCGGCGGCAACGAGGGAATAGTGGACGAACTGCACGTGTACAACACGGCAACCAACCAGTGGTTC ATCCCAGCCGTGAGGGGGGACATTCCCCCTGGGTGTGCAGCCTATGGCTTCGTGTGTGACGGGACTCGCC TCCTGGTGTTTGGTGGGATGGTGGAGTATGGGAAATACAGCAATGACCTCTACGAACTCCAGGCGAGCCG GTGGGAGTGGAAGAGACTCAAAGCAAAGACGCCCAAAAACGGGCCCCCTCCGTGTCCTCGACTCGGGCAC AGCTTCTCCCTTGTGGGCAACAAATGCTACCTGTTTGGGGGTCTGGCCAATGATAGCGAGGACCCAAAGA ACAACATTCCAAGGTACCTGAATGACTTATATATCCTGGAATTACGGCCAGGCTCTGGAGTGGTAGCCTG GGACATTCCCATCACTTACGGGGTCCTACCACCACCCCGGGAGTCACATACTGCCGTGGTCTACACCGAA AAAGACAATAAGAAGTCCAAGCTGGTGATCTACGGCGGGATGAGTGGCTGCAGGCTGGGGGACCTGTGGA CCCTAGATATTGACACCCTGACGTGGAATAAGCCCAGTCTCAGCGGGGTGGCGCCTCTTCCTCGCAGTCT CCACTCGGCAACCACCATCGGAAATAAAATGTACGTGTTTGGTGGCTGGGTGCCTCTCGTCATGGATGAC GTCAAAGTGGCCACACACGAGAAGGAGTGGAAGTGTACCAACACGCTGGCTTGTCTCAACCTGGATACCA TGGCCTGGGAGACCATCCTGATGGATACACTGGAGGACAACATCCCCCGTGCTCGGGCTGGCCACTGCGC AGTCGCCATCAACACCCGCCTGTACATTTGGAGTGGGCGTGACGGCTACCGCAAGGCCTGGAACAACCAG GTCTGCTGCAAGGACCTCTGGTACCTAGAGACAGAAAAGCCACCACCCCCAGCCCGAGTACAACTGGTAC GCGCCAACACCAACTCCCTGGAGGTGAGCTGGGGGGCAGTGGCAACAGCCGACAGCTACCTTCTCCAGCT CCAGAAATATGACATTCCTGCCACGGCTGCTACTGCCACCTCCCCTACACCCAATCCGGTCCCATCTGTG CCTGCCAACCCTCCCAAGAGCCCTGCCCCAGCAGCAGCCGCACCTGCTGTGCAGCCGCTGACCCAAGTAG GCATCACGCTCCTGCCCCAGGCTGCCCCCGCACCCCCGACCACCACCACCATCCAGGTCTTGCCAACGGT GCCTGGCAGCTCCATTTCTGTGCCCACCGCAGCCAGGACTCAAGGTGTCCCTGCTGTTCTCAAAGTGACC GGTCCTCAGGCTACAACAGGAACTCCATTGGTCACCATGCGACCTGCCAGCCAGGCTGGGAAAGCCCCTG TCACCGTGACCTCCCTTCCCGCCGGAGTGCGGATGGTTGTGCCAACACAGAGTGCCCAGGGAACGGTGAT TGGCAGTAGCCCACAGATGAGTGGGATGGCCGCACTGGCCGCTGCGGCCGCTGCCACCCAGAAGATCCCC CCTTCCTCGGCACCCACGGTGCTGAGTGTCCCAGCGGGTACCACCATCGTGAAGACCATGGCTGTGACAC CTGGCACTACCACCCTCCCAGCCACTGTGAAGGTGGCCTCCTCGCCAGTCATGGTGAGCAACCCTGCCAC TCGCATGCTGAAGACTGCAGCCGCCCAGGTGGGGACATCGGTTTCCTCCGCCACCAACACGTCTACCCGC CCTATCATCACAGTGCACAAGTCAGGCACTGTGACAGTGGCCCAGCAAGCCCAGGTGGTGACCACAGTTG TGGGCGGGGTCACCAAGACCATCACCCTGGTGAAGAGCCCCATCTCTGTCCCAGGAGGCAGTGCTCTGAT TTCCAATCTGGGCAAAGTGATGTCGGTGGTCCAGACCAAACCAGTTCAGACTTCAGCAGTCACAGGCCAG GCGTCCACGGGTCCTGTGACTCAGATCATCCAGACCAAAGGGCCCCTGCCAGCGGGAACAATCCTGAAGC TGGTGACCTCAGCAGATGGCAAGCCCACCACCATCATCACTACCACGCAGGCCAGTGGGGCGGGGACCAA GCCCACCATCCTGGGCATCAGCAGCGTCTCCCCCAGTACCACCAAGCCCGGCACGACCACCATCATCAAA ACCATCCCCATGTCGGCCATCATCACCCAGGCGGGCGCCACGGGTGTGACCAGCAGTCCTGGCATCAAGT CCCCCATCACCATCATCACCACCAAGGTGATGACTTCAGGAACTGGAGCACCTGCGAAAATCATCACTGC TGTCCCCAAAATTGCCACTGGCCACGGGCAGCAGGGAGTGACCCAGGTGGTGCTTAAGGGGGCCCCGGGA CAGCCAGGCACCATCCTCCGCACTGTGCCCATGGGGGGTGTTCGCCTGGTCACACCCGTCACCGTCTCCG CCGTCAAGCCAGCCGTCACCACGTTGGTTGTGAAAGGCACCACAGGTGTCACGACCCTAGGCACAGTGAC AGGCACCGTCTCCACCAGCCTTGCCGGGGCGGGGGGCCACAGCACTAGTGCTTCCCTGGCCACGCCCATC ACCACCTTGGGCACCATTGCCACCCTCTCAAGCCAGGTGATCAACCCCACTGCCATCACTGTGTCGGCCG CACAGACCACGCTGACAGCGGCAGGCGGGCTCACAACCCCAACCATCACCATGCAGCCCGTGTCCCAGCC CACCCAGGTAACTCTGATCACGGCACCTAGTGGGGTGGAGGCCCAGCCTGTGCATGACCTCCCTGTGTCC ATTCTGGCCTCCCCGACTACAGAACAGCCCACCGCCACAGTTACCATCGCCGACTCAGGCCAGGGTGATG TGCAGCCTGGCACTGTCACCTTGGTGTGCTCCAACCCACCCTGTGAGACCCACGAGACTGGCACCACCAA CACGGCCACCACTACTGTTGTGGCTAACCTTGGGGGACACCCCCAGCCCACCCAAGTGCAGTTCGTCTGT GACAGACAGGAGGCAGCTGCTTCTCTTGTGACCTCGACTGTGGGCCAGCAGAATGGTAGCGTGGTCCGAG TCTGTTCGAACCCGCCCTGCGAGACCCACGAGACGGGCACCACCAACACCGCCACCACCGCCACCTCCAA CATGGCCGGGCAGCATGGCTGCTCAAACCCACCCTGCGAGACCCACGAGACGGGCACCACCAACACTGCC ACTACAGCCATGTCGAGCGTCGGCGCCAACCACCAGCGAGATGCCCGTCGGGCCTGTGCAGCTGGCACCC CTGCCGTGATCCGGATCAGTGTGGCCACTGGGGCGCTGGAGGCAGCCCAGGGCTCTAAGTCCCAGTGCCA AACCCGCCAGACCAGCGCGACCAGCACCACCATGACTGTGATGGCCACCGGGGCCCCGTGCTCGGCCGGC CCACTCCTTGGGCCGAGCATGGCACGGGAGCCCGGGGGCCGCAGCCCTGCTTTTGTGCAGTTGGCCCCTC TGAGCAGCAAAGTCAGGCTGAGCAGCCCAAGCATTAAGGACCTTCCTGCGGGGCGCCACAGCCATGCGGT CAGCACCGCTGCCATGACCCGTTCCAGCGTGGGTGCTGGGGAGCCCCGCATGGCACCTGTGTGCGAGAGC CTCCAGGGTGGCTCGCCCAGCACCACAGTGACTGTGACAGCCCTGGAGGCACTGCTGTGCCCCTCGGCCA CCGTGACCCAAGTCTGCTCCAACCCACCATGTGAGACCCACGAGACAGGCACCACCAACACCGCCACTAC CTCGAATGCAGGCAGCGCCCAGAGGGTGTGCTCCAACCCGCCATGCGAGACCCACGAGACGGGCACCACC CACACGGCCACCACCGCTACTTCAAACGGGGGCACGGGCCAGCCCGAGGGTGGGCAGCAGCCCCCTGCTG GTCGCCCCTGTGAGACACACCAGACCACTTCCACTGGCACCACCATGTCGGTCAGCGTGGGTGCCCTGCT TCCCGACGCCACTTCTTCCCACAGGACCGTGGAGTCTGGCCTAGAGGTGGCGGCGGCACCCAGCGTCACC CCCCAGGCTGGCACCGCGCTGCTGGCTCCTTTCCCAACACAGAGGGTGTGCTCCAACCCCCCCTGTGAGA CCCACGAGACGGGCACCACTCACACGGCCACCACTGTCACTTCCAACATGAGTTCAAACCAAGACCCCCC ACCTGCTGCCAGCGATCAGGGAGAGGTGGAGAGCACCCAGGGCGACAGCGTGAACATCACCAGCTCCAGT GCCATCACGACAACCGTGTCCTCCACACTGACGCGGGCTGTGACCACCGTGACGCAGTCCACACCGGTCC CGGGCCCCTCTGTGCCGCCCCCAGAGGAACTCCAGGTGTCGCCAGGTCCTCGCCAGCAGCTGCCGCCACG GCAGCTTCTGCAGTCGGCTTCCACAGCCCTGATGGGGGAGTCCGCCGAGGTCCTGTCAGCCTCCCAGACC CCTGAGCTCCCGGCCGCCGTGGATCTGAGCAGCACAGGGGAGCCATCTTCGGGCCAGGAGTCTGCCGGCT CTGCGGTGGTGGCCACTGTGGTGGTCCAGCCACCCCCACCCACACAGTCCGAAGTAGACCAGTTATCACT TCCCCAAGAGCTAATGGCCGAGGCCCAAGCTGGCACCACCACCCTCATGGTAACGGGGCTCACCCCCGAG GAGCTGGCAGTGACGGCTGCTGCAGAAGCAGCTGCCCAGGCCGCAGCCACGGAGGAAGCCCAGGCCCTGG CCATCCAGGCGGTGCTCCAGGCCGCGCAGCAGGCCGTCATGGGCACCGGCGAGCCCATGGACACCTCCGA GGCAGCAGCAACCGTGACTCAGGCGGAGCTGGGGCACCTGTCGGCCGAGGGTCAGGAGGGCCAGGCCACC ACCATACCCATTGTGCTGACACAGCAGGAGCTGGCTGCCCTGGTGCAGCAGCAGCAGCTGCAGGAGGCCC AGGCCCAGCAGCAGCATCACCACCTCCCCACTGAGGCCCTGGCCCCTGCCGACAGTCTCAACGACCCAGC CATTGAGAGCAATTGCCTCAATGAGCTGGCCGGCACGGTCCCCAGCACTGTGGCGCTGCTGCCCTCAACG GCCACTGAGAGCCTGGCTCCATCCAACACATTTGTGGCCCCCCAGCCGGTTGTGGTGGCCAGCCCAGCCA AGCTGCAGGCTGCAGCTACCCTGACCGAAGTGGCCAATGGCATCGAGTCCCTGGGTGTGAAGCCAGACCT GCCGCCCCCACCCAGCAAAGCCCCCATGAAGAAGGAAAACCAGTGGTTTGATGTGGGAGTCATTAAGGGC ACCAATGTAATGGTGACACACTATTTCCTGCCACCAGATGATGCTGTCCCATCAGACGATGATTTGGGCA CCGTCCCTGACTATAACCAGCTGAAGAAGCAGGAGCTGCAGCCAGGCACAGCCTATAAGTTTCGTGTTGC CGGAATCAATGCCTGTGGCCGGGGGCCCTTCAGCGAAATCTCAGCCTTTAAGACGTGCCTGCCTGGTTTC CCAGGGGCCCCTTGTGCCATTAAAATCAGCAAAAGTCCGGATGGTGCTCACCTCACCTGGGAGCCACCCT CTGTGACCTCCGGCAAGATTATCGAGTACTCCGTGTACCTGGCCATCCAGAGCTCACAGGCTGGGGGCGA GCTCAAGAGCTCCACCCCGGCCCAGCTGGCCTTCATGCGGGTGTACTGCGGGCCCAGCCCCTCCTGCCTG GTGCAGTCCTCCAGCCTTTCCAACGCCCACATCGACTACACCACCAAGCCCGCCATCATCTTCCGCATCG CCGCCCGCAATGAGAAGGGCTATGGCCCGGCCACACAAGTGAGGTGGCTGCAGGAAACCAGTAAAGACAG CTCTGGCACCAAGCCAGCCAACAAGCGGCCCATGTCCTCTCCAGAAATGAAATCTGCTCCAAAGAAATCT AAGGCCGATGGTCAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC215341 representing NM_005334 Red=Cloning site Green=Tags(s) MASAVSPANLPAVLLQPRWKRVVGWSGPVPRPRHGHRAVAIKELIVVFGGGNEGIVDELHVYNTATNQWF IPAVRGDIPPGCAAYGFVCDGTRLLVFGGMVEYGKYSNDLYELQASRWEWKRLKAKTPKNGPPPCPRLGH SFSLVGNKCYLFGGLANDSEDPKNNIPRYLNDLYILELRPGSGVVAWDIPITYGVLPPPRESHTAVVYTE KDNKKSKLVIYGGMSGCRLGDLWTLDIDTLTWNKPSLSGVAPLPRSLHSATTIGNKMYVFGGWVPLVMDD VKVATHEKEWKCTNTLACLNLDTMAWETILMDTLEDNIPRARAGHCAVAINTRLYIWSGRDGYRKAWNNQ VCCKDLWYLETEKPPPPARVQLVRANTNSLEVSWGAVATADSYLLQLQKYDIPATAATATSPTPNPVPSV PANPPKSPAPAAAAPAVQPLTQVGITLLPQAAPAPPTTTTIQVLPTVPGSSISVPTAARTQGVPAVLKVT GPQATTGTPLVTMRPASQAGKAPVTVTSLPAGVRMVVPTQSAQGTVIGSSPQMSGMAALAAAAAATQKIP PSSAPTVLSVPAGTTIVKTMAVTPGTTTLPATVKVASSPVMVSNPATRMLKTAAAQVGTSVSSATNTSTR PIITVHKSGTVTVAQQAQVVTTVVGGVTKTITLVKSPISVPGGSALISNLGKVMSVVQTKPVQTSAVTGQ ASTGPVTQIIQTKGPLPAGTILKLVTSADGKPTTIITTTQASGAGTKPTILGISSVSPSTTKPGTTTIIK TIPMSAIITQAGATGVTSSPGIKSPITIITTKVMTSGTGAPAKIITAVPKIATGHGQQGVTQVVLKGAPG QPGTILRTVPMGGVRLVTPVTVSAVKPAVTTLVVKGTTGVTTLGTVTGTVSTSLAGAGGHSTSASLATPI TTLGTIATLSSQVINPTAITVSAAQTTLTAAGGLTTPTITMQPVSQPTQVTLITAPSGVEAQPVHDLPVS ILASPTTEQPTATVTIADSGQGDVQPGTVTLVCSNPPCETHETGTTNTATTTVVANLGGHPQPTQVQFVC DRQEAAASLVTSTVGQQNGSVVRVCSNPPCETHETGTTNTATTATSNMAGQHGCSNPPCETHETGTTNTA TTAMSSVGANHQRDARRACAAGTPAVIRISVATGALEAAQGSKSQCQTRQTSATSTTMTVMATGAPCSAG PLLGPSMAREPGGRSPAFVQLAPLSSKVRLSSPSIKDLPAGRHSHAVSTAAMTRSSVGAGEPRMAPVCES LQGGSPSTTVTVTALEALLCPSATVTQVCSNPPCETHETGTTNTATTSNAGSAQRVCSNPPCETHETGTT HTATTATSNGGTGQPEGGQQPPAGRPCETHQTTSTGTTMSVSVGALLPDATSSHRTVESGLEVAAAPSVT PQAGTALLAPFPTQRVCSNPPCETHETGTTHTATTVTSNMSSNQDPPPAASDQGEVESTQGDSVNITSSS AITTTVSSTLTRAVTTVTQSTPVPGPSVPPPEELQVSPGPRQQLPPRQLLQSASTALMGESAEVLSASQT PELPAAVDLSSTGEPSSGQESAGSAVVATVVVQPPPPTQSEVDQLSLPQELMAEAQAGTTTLMVTGLTPE ELAVTAAAEAAAQAAATEEAQALAIQAVLQAAQQAVMGTGEPMDTSEAAATVTQAELGHLSAEGQEGQAT TIPIVLTQQELAALVQQQQLQEAQAQQQHHHLPTEALAPADSLNDPAIESNCLNELAGTVPSTVALLPST ATESLAPSNTFVAPQPVVVASPAKLQAAATLTEVANGIESLGVKPDLPPPPSKAPMKKENQWFDVGVIKG TNVMVTHYFLPPDDAVPSDDDLGTVPDYNQLKKQELQPGTAYKFRVAGINACGRGPFSEISAFKTCLPGF PGAPCAIKISKSPDGAHLTWEPPSVTSGKIIEYSVYLAIQSSQAGGELKSSTPAQLAFMRVYCGPSPSCL VQSSSLSNAHIDYTTKPAIIFRIAARNEKGYGPATQVRWLQETSKDSSGTKPANKRPMSSPEMKSAPKKS KADGQ TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_005334
ORF Size	6105 bp
OTI Disclaimer	Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_005334.3
RefSeq Size	8436 bp
RefSeq ORF	6108 bp
Locus ID	3054
UniProt ID	P51610
Protein Families	Transcription Factors
MW	208.6 kDa
Gene Summary	This gene is a member of the host cell factor family and encodes a protein with five Kelch repeats, a fibronectin-like motif, and six HCF repeats, each of which contains a highly specific cleavage signal. This nuclear coactivator is proteolytically cleaved at one of the six possible sites, resulting in the creation of an N-terminal chain and the corresponding C-terminal chain. The final form of this protein consists of noncovalently bound N- and C-terminal chains. The protein is involved in control of the cell cycle and transcriptional regulation during herpes simplex virus infection. Alternatively spliced variants which encode different protein isoforms have been described; however, not all variants have been fully characterized. [provided by RefSeq, Jul 2008]

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