mtTFA (TFAM) (NM_003201) Human Tagged ORF Clone
CAT#: RC215488
- TrueORF®
TFAM (Myc-DDK-tagged)-Human transcription factor A, mitochondrial (TFAM), nuclear gene encoding mitochondrial protein
ORF Plasmid: tGFP
"NM_003201" in other vectors (6)
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CNY 3,600.00
CNY 3,705.00
Cited in 2 publications. |
CNY 300.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | MTDPS15; MTTF1; MTTFA; TCF6; TCF6L1; TCF6L2; TCF6L3 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC215488 representing NM_003201
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGTTTCTCCGAAGCATGTGGGGCGTGCTGAGTGCCCTGGGAAGGTCTGGAGCAGAGCTGTGCACCG GCTGTGGAAGTCGACTGCGCTCCCCCTTCAGTTTTGTGTATTTACCGAGGTGGTTTTCATCTGTCTTGGC AAGTTGTCCAAAGAAACCTGTAAGTTCTTACCTTCGATTTTCTAAAGAACAACTACCCATATTTAAAGCT CAGAACCCAGATGCAAAAACTACAGAACTAATTAGAAGAATTGCCCAGCGTTGGAGGGAACTTCCTGATT CAAAGAAAAAAATATATCAAGATGCTTATAGGGCGGAGTGGCAGGTATATAAAGAAGAGATAAGCAGATT TAAAGAACAGCTAACTCCAAGTCAGATTATGTCTTTGGAAAAAGAAATCATGGACAAACATTTAAAAAGG AAAGCTATGACAAAAAAAAAAGAGTTAACACTGCTTGGAAAACCAAAAAGACCTCGTTCAGCTTATAACG TTTATGTAGCTGAAAGATTCCAAGAAGCTAAGGGTGATTCACCGCAGGAAAAGCTGAAGACTGTAAAGGA AAACTGGAAAAATCTGTCTGACTCTGAAAAGGAATTATATATTCAGCATGCTAAAGAGGACGAAACTCGT TATCATAATGAAATGAAGTCTTGGGAAGAACAAATGATTGAAGTTGGACGAAAGGATCTTCTACGTCGCA CAATAAAGAAACAACGAAAATATGGTGCTGAGGAGTGT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC215488 representing NM_003201
Red=Cloning site Green=Tags(s) MAFLRSMWGVLSALGRSGAELCTGCGSRLRSPFSFVYLPRWFSSVLASCPKKPVSSYLRFSKEQLPIFKA QNPDAKTTELIRRIAQRWRELPDSKKKIYQDAYRAEWQVYKEEISRFKEQLTPSQIMSLEKEIMDKHLKR KAMTKKKELTLLGKPKRPRSAYNVYVAERFQEAKGDSPQEKLKTVKENWKNLSDSEKELYIQHAKEDETR YHNEMKSWEEQMIEVGRKDLLRRTIKKQRKYGAEEC TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
Sequencher program is needed, download here. |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_003201 |
ORF Size | 738 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_003201.3 |
RefSeq Size | 1936 bp |
RefSeq ORF | 741 bp |
Locus ID | 7019 |
UniProt ID | Q00059 |
Domains | HMG |
Protein Families | Druggable Genome, Transcription Factors |
Protein Pathways | Huntington's disease |
MW | 29.1 kDa |
Gene Summary | This gene encodes a key mitochondrial transcription factor containing two high mobility group motifs. The encoded protein also functions in mitochondrial DNA replication and repair. Sequence polymorphisms in this gene are associated with Alzheimer's and Parkinson's diseases. There are pseudogenes for this gene on chromosomes 6, 7, and 11. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2012] |
Citations (2)
The use of this cDNA Clones has been cited in the following citations: |
---|
Low mitochondrial DNA copy number induces chemotherapy resistance via epithelial-mesenchymal transition by DNA methylation in esophageal squamous cancer cells
,null,
Journal of Translational Medicine
,PubMed ID 36038893
[mtTFA]
|
EBV reduces autophagy, intracellular ROS and mitochondria to impair monocyte survival and differentiation
,Gilardini Montani, MS;Santarelli, R;Granato, M;Gonnella, R;Torrisi, MR;Faggioni, A;Cirone, M;,
Autophagy
,PubMed ID 30324853
[mtTFA]
|
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC215488L1 | Lenti ORF clone of Human transcription factor A, mitochondrial (TFAM), nuclear gene encoding mitochondrial protein, Myc-DDK-tagged |
CNY 5,890.00 |
|
RC215488L2 | Lenti ORF clone of Human transcription factor A, mitochondrial (TFAM), nuclear gene encoding mitochondrial protein, mGFP tagged |
CNY 5,890.00 |
|
RC215488L3 | Lenti ORF clone of Human transcription factor A, mitochondrial (TFAM), nuclear gene encoding mitochondrial protein, Myc-DDK-tagged |
CNY 5,890.00 |
|
RC215488L4 | Lenti ORF clone of Human transcription factor A, mitochondrial (TFAM), nuclear gene encoding mitochondrial protein, mGFP tagged |
CNY 5,890.00 |
|
RG215488 | TFAM (tGFP-tagged) - Human transcription factor A, mitochondrial (TFAM), nuclear gene encoding mitochondrial protein |
CNY 4,370.00 |
|
SC118128 | TFAM (untagged)-Human transcription factor A, mitochondrial (TFAM), nuclear gene encoding mitochondrial protein |
CNY 3,600.00 |