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LRRFIP1 (NM_004735) Human Tagged ORF Clone
CAT＃: RC216265
LRRFIP1 (Myc-DDK-tagged)-Human leucine rich repeat (in FLII) interacting protein 1 (LRRFIP1), transcript variant 4
ORF Plasmid: tGFP
"NM_004735" in other vectors (6)
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CNY 5,744.00
CNY 8,270.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | FLAP-1; FLAP1; FLIIAP1; GCF-2; GCF2; HUFI-1; TRIP |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC216265 representing NM_004735
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACCAGCCCCGCGGCCGCTCAAAGCCGGGAGATCGACTGTTTGAGCCCGGAAGCGCAGAAGCTGGCGG AAGCCCGGCTCGCTGCAAAACGGGCGGCCCGCGCGGAGGCTCGCGAGATCCGCATGAAGGAGCTGGAGCG GCAGCAGAAGGAGGAAGACAGTGAGCGCTACTCTCGTAGATCCAGAAGAAACACATCGGCTTCTGATGAA GACGAGCGCATGTCAGTGGGTAGTCGTGGAAGCCTGAGGGTAGAAGAGAGACCAGAAAAAGATTTTACTG AGAAGGGGTCTCGTAACATGCCGGGCCTGTCTGCAGCCACGCTGGCCTCTCTGGGTGGGACTTCCTCTCG GAGAGGCAGCGGAGACACCTCCATCTCCATCGACACCGAGGCATCCATCAGGGAAATCAAGGACTCTCTA GCAGAAGTTGAAGAGAAATATAAGAAGGCTATGGTTTCCAATGCTCAGCTAGACAATGAAAAGACAAACT TCATGTACCAGGTTGATACCCTAAAAGATATGTTGCTGGAGCTTGAAGAACAGCTGGCTGAATCTAGGCG GCAGTACGAAGAGAAAAACAAAGAATTTGAAAGGGAAAAACACGCCCACAGTATACTGCAATTTCAGTTT GCTGAAGTCAAGGAGGCCCTGAAGCAAAGAGAGGAAATGCTCGAGAAACATGGAATAATCCTAAATTCAG AAATAGCTACCAATGGAGAGACTTCCGACACCCTCAATAATGTTGGATACCAAGGTCCTACCAAGATGAC AAAAGAAGAGTTAAATGCCCTCAAGTCGACAGGGGATGGGACCCTAGGAAGAGCCAGTGAAGTGGAGGTG AAAAATGAAATCGTGGCGAATGTGGGGAAAAGAGAAATCTTGCACAATACTGAGAAAGAACAACACACAG AGGACACAGTGAAGGACTGTGTGGACATAGAGGTATTCCCTGCTGGTGAGAATACCGAGGACCAGAAATC CTCTGAAGACACTGCCCCATTCCTAGGAACCTTAGCAGGTGCTACCTATGAGGAACAGGTTCAAAGCCAA ATTCTTGAGAGCAGTTCTCTCCCTGAAAACACAGTACAGGTTGAGTCAAATGAGGTCATGGGTGCACCAG ATGACAGGACCAGAACTCCCCTTGAGCCATCCAACTGTTGGAGTGACTTAGATGGTGGGAACCACACAGA GAATGTGGGAGAGGCAGCAGTGACTCAGGTTGAAGAGCAGGCAGGCACAGTGGCCTCGTGTCCTTTAGGG CATAGTGATGACACAGTTTATCATGATGACAAATGTATGGTAGAGGTCCCCCAAGAGTTAGAGACAAGCA CAGGGCATAGTTTAGAGAAAGAATTCACCAACCAGGAAGCAGCTGAGCCCAAGGAGGTTCCAGCGCACAG TACAGAAGTAGGTAGGGATCACAACGAAGAAGAGGGTGAAGAAACAGGATTAAGGGACGAGAAACCAATC AAGACAGAAGTTCCTGGTTCTCCAGCAGGAACTGAGGGCAACTGTCAGGAAGCGACAGGTCCAAGTACAG TAGACACTCAAAATGAACCCTTAGATATGAAAGAGCCCGATGAAGAAAAGAGTGACCAACAGGGAGAGGC ATTGGACTCATCGCAGAAGAAGACAAAGAACAAGAAAAAGAAAAACAAGAAGAAAAAATCCCCAGTACCC GTAGAAACCCTTAAAGATGTTAAAAAAGAGTTAACGTATCAGAACACAGATTTAAGTGAAATTAAGGAAG AAGAGCAGGTAAAGTCTACTGACAGAAAGTCAGCAGTGGAAGCCCAAAACGAGGTGACTGAAAATCCAAA ACAGAAAATTGCAGCAGAAAGCAGTGAAAATGTTGATTGTCCGGAGAATCCTAAAATTAAGTTGGATGGA AAACTTGACCAAGAAGGTGATGATGTACAAACAGCAGCTGAGGAGGTACTAGCTGATGGAGACACATTAG ATTTTGAGGATGACACCGTTCAATCATCAGGCCCGAGGGCTGGTGGTGAAGAATTAGATGAAGGTGTTGC AAAAGATAATGCTAAAATAGATGGTGCCACTCAAAGCAGTCCTGCAGAACCAAAGAGCGAAGACGCAGAT CGCTGCACCCTGCCCGAACATGAAAGTCCCTCACAGGACATTAGTGATGCCTGTGAAGCAGAAAGTACAG AGAGGTGTGAGATGTCAGAACATCCAAGTCAGACCGTCAGGAAAGCTTTAGACAGCAATAGCCTAGAGAA CGATGACTTGTCGGCACCAGGAAGAGAGCCAGGGCACTTCAATCCAGAAAGCAGAGAAGATACCAGAGGA GGGAATGAGAAGGGCAAAAGCAAAGAAGACTGTACCATGTCC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC216265 representing NM_004735
Red=Cloning site Green=Tags(s) MTSPAAAQSREIDCLSPEAQKLAEARLAAKRAARAEAREIRMKELERQQKEEDSERYSRRSRRNTSASDE DERMSVGSRGSLRVEERPEKDFTEKGSRNMPGLSAATLASLGGTSSRRGSGDTSISIDTEASIREIKDSL AEVEEKYKKAMVSNAQLDNEKTNFMYQVDTLKDMLLELEEQLAESRRQYEEKNKEFEREKHAHSILQFQF AEVKEALKQREEMLEKHGIILNSEIATNGETSDTLNNVGYQGPTKMTKEELNALKSTGDGTLGRASEVEV KNEIVANVGKREILHNTEKEQHTEDTVKDCVDIEVFPAGENTEDQKSSEDTAPFLGTLAGATYEEQVQSQ ILESSSLPENTVQVESNEVMGAPDDRTRTPLEPSNCWSDLDGGNHTENVGEAAVTQVEEQAGTVASCPLG HSDDTVYHDDKCMVEVPQELETSTGHSLEKEFTNQEAAEPKEVPAHSTEVGRDHNEEEGEETGLRDEKPI KTEVPGSPAGTEGNCQEATGPSTVDTQNEPLDMKEPDEEKSDQQGEALDSSQKKTKNKKKKNKKKKSPVP VETLKDVKKELTYQNTDLSEIKEEEQVKSTDRKSAVEAQNEVTENPKQKIAAESSENVDCPENPKIKLDG KLDQEGDDVQTAAEEVLADGDTLDFEDDTVQSSGPRAGGEELDEGVAKDNAKIDGATQSSPAEPKSEDAD RCTLPEHESPSQDISDACEAESTERCEMSEHPSQTVRKALDSNSLENDDLSAPGREPGHFNPESREDTRG GNEKGKSKEDCTMS TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_004735 |
| ORF Size | 2352 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_004735.4 |
| RefSeq Size | 3648 bp |
| RefSeq ORF | 2355 bp |
| Locus ID | 9208 |
| UniProt ID | Q32MZ4 |
| Protein Families | Transcription Factors |
| MW | 86.2 kDa |
| Gene Summary | Transcriptional repressor which preferentially binds to the GC-rich consensus sequence (5'-AGCCCCCGGCG-3') and may regulate expression of TNF, EGFR and PDGFA. May control smooth muscle cells proliferation following artery injury through PDGFA repression. May also bind double-stranded RNA. Positively regulates Toll-like receptor (TLR) signaling in response to agonist probably by competing with the negative FLII regulator for MYD88-binding.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC216265L1 | Lenti ORF clone of Human leucine rich repeat (in FLII) interacting protein 1 (LRRFIP1), transcript variant 4, Myc-DDK-tagged |
CNY 8,144.00 |
|
| RC216265L2 | Lenti ORF clone of Human leucine rich repeat (in FLII) interacting protein 1 (LRRFIP1), transcript variant 4, mGFP tagged |
CNY 10,170.00 |
|
| RC216265L3 | Lenti ORF clone of Human leucine rich repeat (in FLII) interacting protein 1 (LRRFIP1), transcript variant 4, Myc-DDK-tagged |
CNY 10,170.00 |
|
| RC216265L4 | Lenti ORF clone of Human leucine rich repeat (in FLII) interacting protein 1 (LRRFIP1), transcript variant 4, mGFP tagged |
CNY 10,170.00 |
|
| RG216265 | LRRFIP1 (tGFP-tagged) - Human leucine rich repeat (in FLII) interacting protein 1 (LRRFIP1), transcript variant 4 |
CNY 9,120.00 |
|
| SC117183 | LRRFIP1 (untagged)-Human leucine rich repeat (in FLII) interacting protein 1 (LRRFIP1), transcript variant 4 |
CNY 5,752.00 |
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