CBX2 (NM_032647) Human Tagged ORF Clone
CAT#: RC216313
CBX2 (Myc-DDK-tagged)-Human chromobox homolog 2 (CBX2), transcript variant 2
ORF Plasmid: tGFP
"NM_032647" in other vectors (6)
Need custom modification / cloning service?
Get a free quote
CNY 2,400.00
CNY 3,705.00
Cited in 3 publications. |
CNY 300.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | CDCA6; M33; SRXY5 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC216313 representing NM_032647
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGGAGCTGAGCAGCGTGGGCGAGCAGGTCTTCGCCGCCGAGTGCATCCTGAGCAAGCGGCTCCGCA AGGGCAAGCTGGAGTACCTGGTCAAGTGGCGCGGCTGGTCCTCCAAACATAACAGCTGGGAGCCGGAGGA GAACATCCTGGACCCGAGGCTGCTCCTGGCCTTCCAGAAGAAGGAACATGAGAAGGAGGTGCAGAACCGG AAGAGAGGCAAGAGGCCGAGAGGCCGGCCAAGGAAGCTCACTGCCATGTCCTCCTGCAGCCGGCGCTCCA AGCTCAAGGTGGGTGGCTGCGCTGGGTATGCTGACCCCACCTCCCAGCACCCCCTTGGCGTAGGGGGCAG GCAGAGGGAGGGTTTGGGGCCCTCAGGAAGGGGGTGGCACTTCTGCCAACAGTCTGTCCCTCTACTCGGA AAACAGGAGCCCCCTTTCTTCCTGTCTCTCAGCTTCTGCTGCCAGGGGCCCCAGCCGGCTGAGAGTTCCT CCCCGCCCCTCCCAGGGGCTTCCTGCTTCAGCCTGTCCTGCACGCCTCTCTGCTGGGTGGCAGGGTCAAA CTGCTGCAGACAAGCACTCTTCCCTCCCAGGGGGTCCTTGGGGGACGGAAAGGAACAGGAAGCATGCGTA CAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC216313 representing NM_032647
Red=Cloning site Green=Tags(s) MEELSSVGEQVFAAECILSKRLRKGKLEYLVKWRGWSSKHNSWEPEENILDPRLLLAFQKKEHEKEVQNR KRGKRPRGRPRKLTAMSSCSRRSKLKVGGCAGYADPTSQHPLGVGGRQREGLGPSGRGWHFCQQSVPLLG KQEPPFFLSLSFCCQGPQPAESSSPPLPGASCFSLSCTPLCWVAGSNCCRQALFPPRGSLGDGKEQEACV Q TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
Sequencher program is needed, download here. |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_032647 |
ORF Size | 633 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_032647.3 |
RefSeq Size | 1061 bp |
RefSeq ORF | 636 bp |
Locus ID | 84733 |
UniProt ID | Q14781 |
Protein Families | Transcription Factors |
MW | 23.1 kDa |
Gene Summary | This gene encodes a component of the polycomb multiprotein complex, which is required to maintain the transcriptionally repressive state of many genes throughout development via chromatin remodeling and modification of histones. Disruption of this gene in mice results in male-to-female gonadal sex reversal. Mutations in this gene are also associated with gonadal dysgenesis in humans. Alternatively spliced transcript variants encoding different isoforms have been noted for this gene.[provided by RefSeq, Mar 2010] |
Citations (3)
The use of this cDNA Clones has been cited in the following citations: |
---|
The transcriptional regulator CBX2 and ovarian function: A whole genome and whole transcriptome approach
,Bouazzi, L;Sproll, P;Eid, W;Biason-Lauber, A;,
Sci Rep
,PubMed ID 31745224
[CBX2]
|
Assembling the jigsaw puzzle: CBX2 isoform 2 and its targets in disorders/differences of sex development
,Sproll, P;Eid, W;Gomes, CR;Mendonca, BB;Gomes, NL;Costa, EM;Biason-Lauber, A;,
Mol Genet Genomic Med
,PubMed ID 29998616
[CBX2]
|
CBX2 and the Ovary: Novel Insights into Regulatory Networks in Humans
,Bouazzi, L;Franco, M;Maret, A;Biason-Lauber, A;,
Journal of Gynecology & Reproductive Medicine
[CBX2]
|
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC216313L1 | Lenti ORF clone of Human chromobox homolog 2 (CBX2), transcript variant 2, Myc-DDK-tagged |
CNY 4,800.00 |
|
RC216313L2 | Lenti ORF clone of Human chromobox homolog 2 (CBX2), transcript variant 2, mGFP tagged |
CNY 5,890.00 |
|
RC216313L3 | Lenti ORF clone of Human chromobox homolog 2 (CBX2), transcript variant 2, Myc-DDK-tagged |
CNY 5,890.00 |
|
RC216313L4 | Lenti ORF clone of Human chromobox homolog 2 (CBX2), transcript variant 2, mGFP tagged |
CNY 5,890.00 |
|
RG216313 | CBX2 (tGFP-tagged) - Human chromobox homolog 2 (CBX2), transcript variant 2 |
CNY 4,000.00 |
|
SC123042 | CBX2 (untagged)-Human chromobox homolog 2 (CBX2), transcript variant 2 |
CNY 2,400.00 |