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DDX42 (NM_203499) Human Tagged ORF Clone
CAT＃: RC216636
DDX42 (Myc-DDK-tagged)-Human DEAD (Asp-Glu-Ala-Asp) box polypeptide 42 (DDX42), transcript variant 2
ORF Plasmid: tGFP
"NM_203499" in other vectors (4)
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CNY 3,990.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | DDX42P; RHELP; RNAHP; SF3B8; SF3b125 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC216636 representing NM_203499
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAACTGGAATAAAGGTGGTCCTGGCACTAAGCGAGGATTTGGCTTTGGAGGTTTTGCCATCAGTGCTG GGAAAAAGGAGGAACCCAAACTCCCACAGCAGTCCCACAGTGCCTTTGGGGCAACCAGCTCTTCTTCTGG ATTTGGAAAGTCAGCTCCACCACAGCTTCCTTCTTTCTACAAAATTGGATCTAAGCGGGCCAACTTTGAT GAAGAAAATGCCTATTTTGAAGATGAGGAAGAAGATTCTAGCAACGTTGATTTACCTTACATTCCTGCTG AAAACTCACCAACTCGCCAGCAATTCCATTCCAAGCCAGTAGATTCTGACAGCGATGATGATCCCTTGGA GGCATTCATGGCTGAAGTGGAGGATCAGGCAGCTAGAGACATGAAGAGGCTTGAAGAAAAGGACAAGGAA AGAAAAAACGTAAAGGGTATTCGAGATGACATTGAAGAGGAAGATGACCAAGAAGCTTATTTTCGATACA TGGCAGAAAACCCAACTGCTGGTGTGGTTCAGGAGGAAGAGGAAGACAATCTAGAATATGATAGTGACGG AAATCCAATTGCACCTACCAAAAAAATCATTGATCCTCTTCCCCCCATTGATCATTCAGAGATTGACTAT CCACCATTTGAAAAAAACTTTTACAATGAGCATGAAGAGATAACCAACCTCACTCCACAGCAGTTAATAG ATCTCCGGCATAAGCTCAATCTTCGGGTCTCTGGTGCTGCACCTCCTAGACCAGGAAGTAGCTTTGCTCA TTTTGGGTTTGACGAACAACTTATGCACCAGATTCGGAAATCTGAATACACACAGCCCACTCCAATACAG TGCCAGGGTGTGCCTGTGGCATTAAGTGGTAGAGACATGATTGGTATTGCCAAAACAGGTAGTGGGAAAA CTGCAGCCTTCATTTGGCCCATGTTGATTCATATAATGGACCAGAAGGAGTTGGAACCAGGTGATGGACC AATTGCAGTGATTGTGTGTCCTACCAGGGAGCTTTGCCAGCAGATCCATGCAGAATGTAAGCGGTTTGGA AAAGCATATAATCTTCGATCAGTGGCCGTATATGGAGGAGGGAGTATGTGGGAGCAGGCCAAGGCCCTTC AGGAGGGGGCAGAGATTGTTGTGTGTACCCCAGGTCGACTGATAGATCATGTGAAAAAGAAAGCTACCAA TCTTCAAAGAGTCTCTTACCTTGTGTTTGATGAAGCAGATCGAATGTTTGACATGGGATTTGAGTACCAA GTTCGATCCATAGCAAGTCATGTTCGTCCTGACAGGCAGACTCTCTTATTTAGTGCAACTTTTCGGAAGA AGATTGAAAAGTTGGCCAGAGACATCCTGATCGACCCTATTCGAGTGGTGCAGGGAGATATTGGAGAGGC AAATGAAGATGTGACACAGATTGTGGAGATTCTCCATTCTGGACCTAGTAAATGGAACTGGCTTACCCGG CGTCTGGTAGAATTTACCTCTTCAGGGAGTGTCCTCCTCTTTGTTACTAAAAAAGCCAATGCTGAAGAGC TAGCGAATAACCTTAAACAGGAGGGTCATAATCTTGGGCTGCTCCATGGGGATATGGATCAGAGTGAGAG AAACAAGGTCATTTCAGACTTTAAGAAAAAGGACATCCCAGTCCTGGTGGCCACAGATGTTGCAGCCCGT GGTCTGGACATTCCTTCAATTAAGACTGTCATTAACTATGATGTGGCACGAGACATTGATACCCACACGC ATAGGATTGGCCGCACAGGAAGAGCGGGTGAGAAAGGTGTGGCCTATACCCTACTCACTCCCAAGGACAG CAATTTTGCTGGTGACCTGGTCCGGAACTTGGAAGGAGCCAATCAACACGTTTCTAAGGAACTCCTAGAT CTGGCAATGCAGAATGCCTGGTTTCGGAAATCTCGATTCAAAGGAGGGAAAGGAAAAAAGCTGAACATTG GTGGAGGAGGCCTAGGCTACAGGGAGCGGCCTGGCCTGGGCTCTGAGAACATGGATCGAGGAAATAACAA TGTAATGAGCAATTATGAGGCCTACAAGCCTTCCACAGGAGCTATGGGAGATCGACTAACGGCAATGAAA GCAGCTTTCCAGTCACAGTACAAGAGTCACTTTGTTGCAGCCAGTTTAAGTAATCAGAAGGCTGGAAGTT CTGCTGCTGGGGCAAGTGGGTGGACTAGTGCAGGGAGCTTGAATTCTGTTCCAACTAACTCAGCACAACA GGGCCATAACAGTCCTGACAGCCCCGTCACCAGTGCCGCCAAGGGCATCCCAGGCTTTGGCAATACTGGC AACATCAGTGGTGCCCCTGTGACCTACCCGTCTGCCGGAGCCCAAGGAGTCAACAACACAGCTTCAGGGA ATAACAGCCGAGAAGGGACTGGGGGCAGCAACGGGAAAAGAGAGAGATATACTGAGAACCGGGGCAGCAG CCGTCACAGTCACGGAGAGACTGGCAATCGGCATAGCGATAGTCCACGTCACGGAGATGGTGGTCGCCAT GGAGATGGATACCGCCATCCAGAAAGCAGCAGCCGTCATACTGATGGCCATCGGCACGGGGAGAACAGAC ATGGAGGAAGCGCAGGCCGGCATGGGGAGAACCGGGGTGCAAATGATGGTCGGAATGGGGAAAGCAGGAA AGAAGCTTTTAATCGTGAGAGCAAGATGGAGCCCAAGATGGAACCCAAAGTGGACAGCAGCAAGATGGAC AAGGTGGACAGCAAGACAGATAAGACAGCTGACGGCTTTGCTGTCCCAGAGCCGCCTAAACGCAAGAAAA GTCGATGGGACAGT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC216636 representing NM_203499
Red=Cloning site Green=Tags(s) MNWNKGGPGTKRGFGFGGFAISAGKKEEPKLPQQSHSAFGATSSSSGFGKSAPPQLPSFYKIGSKRANFD EENAYFEDEEEDSSNVDLPYIPAENSPTRQQFHSKPVDSDSDDDPLEAFMAEVEDQAARDMKRLEEKDKE RKNVKGIRDDIEEEDDQEAYFRYMAENPTAGVVQEEEEDNLEYDSDGNPIAPTKKIIDPLPPIDHSEIDY PPFEKNFYNEHEEITNLTPQQLIDLRHKLNLRVSGAAPPRPGSSFAHFGFDEQLMHQIRKSEYTQPTPIQ CQGVPVALSGRDMIGIAKTGSGKTAAFIWPMLIHIMDQKELEPGDGPIAVIVCPTRELCQQIHAECKRFG KAYNLRSVAVYGGGSMWEQAKALQEGAEIVVCTPGRLIDHVKKKATNLQRVSYLVFDEADRMFDMGFEYQ VRSIASHVRPDRQTLLFSATFRKKIEKLARDILIDPIRVVQGDIGEANEDVTQIVEILHSGPSKWNWLTR RLVEFTSSGSVLLFVTKKANAEELANNLKQEGHNLGLLHGDMDQSERNKVISDFKKKDIPVLVATDVAAR GLDIPSIKTVINYDVARDIDTHTHRIGRTGRAGEKGVAYTLLTPKDSNFAGDLVRNLEGANQHVSKELLD LAMQNAWFRKSRFKGGKGKKLNIGGGGLGYRERPGLGSENMDRGNNNVMSNYEAYKPSTGAMGDRLTAMK AAFQSQYKSHFVAASLSNQKAGSSAAGASGWTSAGSLNSVPTNSAQQGHNSPDSPVTSAAKGIPGFGNTG NISGAPVTYPSAGAQGVNNTASGNNSREGTGGSNGKRERYTENRGSSRHSHGETGNRHSDSPRHGDGGRH GDGYRHPESSSRHTDGHRHGENRHGGSAGRHGENRGANDGRNGESRKEAFNRESKMEPKMEPKVDSSKMD KVDSKTDKTADGFAVPEPPKRKKSRWDS TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_203499 |
| ORF Size | 2814 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_203499.3 |
| RefSeq Size | 3943 bp |
| RefSeq ORF | 2817 bp |
| Locus ID | 11325 |
| UniProt ID | Q86XP3 |
| Protein Pathways | Spliceosome |
| MW | 103 kDa |
| Gene Summary | This gene encodes a member of the Asp-Glu-Ala-Asp (DEAD) box protein family. Members of this protein family are putative RNA helicases, and are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. Two transcript variants encoding the same protein have been identified for this gene. [provided by RefSeq, Jul 2008] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC216636L3 | Lenti-ORF clone of DDX42 (Myc-DDK-tagged)-Human DEAD (Asp-Glu-Ala-Asp) box polypeptide 42 (DDX42), transcript variant 2 |
CNY 5,890.00 |
|
| RC216636L4 | Lenti-ORF clone of DDX42 (mGFP-tagged)-Human DEAD (Asp-Glu-Ala-Asp) box polypeptide 42 (DDX42), transcript variant 2 |
CNY 5,890.00 |
|
| RG216636 | DDX42 (tGFP-tagged) - Human DEAD (Asp-Glu-Ala-Asp) box polypeptide 42 (DDX42), transcript variant 2 |
CNY 4,370.00 |
|
| SC308214 | DDX42 (untagged)-Human DEAD (Asp-Glu-Ala-Asp) box polypeptide 42 (DDX42), transcript variant 2 |
CNY 7,220.00 |
