MAP2 (NM_002374) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	MAP-2; MAP2A; MAP2B; MAP2C
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC216775 representing NM_002374 Red=Cloning site Blue=ORF Green=Tags(s) CTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCAGATGAACGGAAAGACGAAGCAAAGGCACCTCACTGGACCTCAGCACCGCTAACAGAGGCATCTG CACACTCACATCCACCTGAGATTAAGGATCAAGGCGGAGCAGGGGAAGGACTTGTCCGAAGCGCCAATGG ATTCCCATACAGGGAGGATGAAGAGGGTGCCTTTGGAGAGCATGGGTCACAGGGCACCTATTCAAATACC AAAGAGAATGGGATCAACGGAGAGCTGACCTCAGCTGACAGAGAAACAGCAGAGGAGGTGTCTGCAAGGA TAGTTCAAGTAGTCACTGCTGAGGCTGTAGCAGTCCTGAAAGGTGAACAAGAGAAAGAAGCTCAACATAA AGACCAGACTGCAGCTCTGCCTTTAGCAGCTGAAGAAACAGCTAATCTGCCTCCTTCTCCACCCCCATCA CCTGCCTCAGAACAGACTGTCACAGTGGAGGAAGATTTACTTACAGCCTCGAAGATGGAGTTCCACGATC AACAGGAATTGACTCCCTCTACAGCTGAGCCTTCAGACCAGAAGGAAAAGGAGTCAGAGAAGCAAAGTAA GCCTGGTGAAGACCTTAAACATGCTGCCTTAGTTTCTCAGCCAGAGACAACTAAAACTTACCCTGATAAA AAGGACATGCAAGGCACAGAAGAAGAAAAAGCACCCCTAGCTTTGTTTGGGCACACTCTTGTTGCCAGCC TGGAAGACATGAAACAGAAGACAGAACCAAGCCTTGTAGTACCTGGCATTGACCTCCCTAAAGAGCCTCC AACTCCAAAAGAACAAAAGGACTGGTTCATCGAAATGCCAACGGAAGCAAAAAAGGATGAGTGGGGTTTA GTTGCCCCCATATCTCCTGGCCCTCTGACTCCCATGAGGGAAAAAGATGTATTTGATGATATCCCAAAAT GGGAAGGGAAACAGTTTGATTCTCCCATGCCAAGTCCCTTTCAAGGGGGAAGCTTCACTCTTCCTTTAGA TGTCATGAAGAATGAAATAGTTACAGAAACATCGCCCTTTGCCCCTGCCTTTTTACAGCCAGATGACAAA AAATCTCTGCAACAAACCAGTGGCCCAGCTACTGCCAAAGATAGTTTTAAAATTGAAGAGCCCCATGAGG CTAAACCTGACAAAATGGCAGAAGCACCACCCTCAGAGGCAATGACCTTACCCAAAGATGCTCACATTCC AGTTGTAGAAGAACATGTTATGGGGAAAGTTTTAGAGGAAGAAAAGGAGGCCATAAATCAAGAGACTGTG CAGCAAAGGGATACTTTCACCCCCAGTGGACAGGAACCTATACTTACTGAAAAGGAAACTGAGCTGAAGC TTGAAGAAAAAACCACCATTTCTGACAAAGAAGCTGTGCCAAAAGAGAGTAAACCCCCAAAACCTGCAGA TGAAGAAATAGGCATAATTCAGACCTCCACAGAGCACACTTTCTCAGAACAGAAAGACCAAGAGCCTACC ACAGATATGTTGAAACAGGACTCGTTCCCTGTAAGTTTGGAGCAAGCAGTTACAGATTCAGCCATGACCT CTAAAACACTGGAGAAAGCCATGACCGAACCATCTGCATTAATTGAAAAGAGCTCAATTCAGGAACTTTT TGAAATGAGAGTTGATGACAAAGATAAGATTGAAGGAGTTGGAGCTGCAACATCAGCTGAGCTTGATATG CCATTTTATGAAGATAAATCAGGAATGTCCAAGTACTTTGAAACATCTGCCTTGAAAGAAGAAGCAACAA AAAGCATTGAGCCAGGCAGTGATTACTATGAACTGAGTGACACTAGAGAAAGTGTCCATGAGTCTATTGA TACCATGTCTCCCATGCATAAAAATGGTGACAAGGAGTTTCAAACAGGAAAAGAATCCCAGCCCAGTCCT CCAGCACAAGAAGCAGGGTACAGCACTCTCGCACAGAGTTATCCATCAGATTTACCTGAAGAACCCAGTT CTCCTCAAGAAAGAATGTTCACTATTGATCCAAAAGTGTATGGAGAGAAAAGGGACCTCCACAGTAAGAA TAAGGATGATTTGACCCTTAGCAGGAGTTTAGGACTTGGTGGTAGGTCTGCAATAGAACAAAGAAGCATG TCAATCAATTTGCCGATGTCTTGCCTAGATTCCATAGCCCTTGGATTTAACTTTGGTCGGGGACATGATC TTTCTCCTCTGGCTTCCGATATTCTAACCAACACTAGTGGAAGTATGGATGAAGGGGATGATTACCTTCC AGCCACCACACCTGCACTGGAGAAAGCCCCTTGCTTCCCTGTAGAAAGCAAAGAGGAAGAACAGATAGAG AAAGTAAAAGCTACTGGAGAAGAAAGTACTCAAGCGGAGATATCATGTGAGTCTCCTTTCCTAGCCAAAG ATTTTTACAAAAATGGTACTGTCATGGCACCTGACCTTCCTGAAATGCTAGATCTGGCAGGCACAAGGTC AAGATTGGCTTCTGTGAGTGCAGATGCTGAGGTTGCCAGGAGGAAATCAGTCCCATCAGAGACTGTGGTT GAGGATAGTCGTACTGGCTTGCCCCCGGTAACTGATGAAAACCATGTCATTGTAAAAACGGACAGTCAGC TCGAAGACCTGGGCTACTGTGTGTTCAATAAGTACACAGTCCCATTGCCATCACCTGTTCAAGACAGTGA GAATTTATCAGGGGAGAGTGGTACCTTTTACGAAGGCACTGATGATAAAGTTCGAAGAGATTTGGCCACA GACCTTTCACTGATTGAAGTGAAACTGGCAGCAGCCGGAAGAGTCAAAGATGAGTTCAGTGTTGACAAAG AAGCATCCGCGCATATCTCTGGTGACAAATCAGGACTGAGTAAGGAGTTTGACCAAGAGAAGAAAGCTAA TGATAGGTTGGATACTGTACTAGAAAAGAGTGAAGAACATGCTGATTCAAAAGAACATGCCAAGAAAACT GAAGAGGCTGGTGATGAAATAGAAACATTCGGATTAGGAGTAACCTATGAGCAAGCTTTGGCCAAAGATT TGTCAATACCAACAGATGCATCCTCTGAGAAAGCAGAGAAGGGTCTTAGTTCAGTTCCAGAGATAGCTGA GGTAGAACCATCCAAAAAGGTGGAACAAGGTCTGGATTTTGCTGTCCAGGGTCAACTAGATGTTAAAATT AGTGACTTTGGACAGATGGCTTCAGGGCTAAACATAGATGATAGAAGGGCAACAGAGCTAAAACTTGAGG CTACACAGGACATGACCCCCTCATCCAAAGCACCGCAGGAGGCAGATGCATTTATGGGTGTTGAGTCTGG CCACATGAAAGAAGGCACTAAAGTTAGTGAGACAGAAGTCAAACAGAAGGTGGCCAAGCCTGACTTGGTG CACCAGGAGGCTGTAGACAAGGAGGAGTCCTATGAATCTAGTGGTGAGCATGAAAGTCTCACCATGGAGT CCTTGAAAGCTGATGAGGGCAAGAAGGAAACATCTCCAGAATCATCTCTAATTCAAGATGAGATTGCCGT CAAATTGTCAGTGGAAATACCTTGCCCACCTGCTGTTTCAGAGGCTGATTTAGCCACAGATGAGAGAGCT GATGTCCAGATGGAATTTATTCAGGGGCCAAAAGAAGAAAGCAAAGAGACCCCAGATATATCCATCACGC CTTCTGATGTTGCAGAGCCATTGCATGAAACGATCGTATCTGAACCAGCAGAGATTCAGAGTGAGGAAGA AGAGATAGAAGCCCAGGGAGAATATGATAAACTGCTCTTCCGCTCAGACACCCTTCAGATAACTGACCTG GGTGTCTCAGGTGCCAGGGAGGAATTTGTGGAGACCTGCCCAAGTGAACACAAAGGAGTGATTGAGTCTG TTGTGACCATCGAGGATGATTTCATCACTGTAGTGCAAACCACAACTGATGAAGGGGAGTCAGGGTCCCA CAGCGTGCGTTTTGCAGCCCTAGAGCAGCCTGAGGTGGAAAGGAGACCATCTCCTCATGATGAAGAAGAG TTTGAAGTAGAAGAGGCAGCTGAAGCCCAGGCAGAACCCAAAGATGGTTCCCCAGAGGCTCCAGCTTCCC CTGAGAGAGAAGAGGTTGCACTTTCTGAATATAAGACAGAAACCTATGACGATTACAAAGATGAGACCAC CATTGACGACTCCATCATGGACGCTGACAGCCTCTGGGTGGACACTCAAGATGATGATAGGAGCATCATG ACAGAACAGTTAGAAACTATTCCTAAAGAGGAGAAAGCTGAAAAGGAAGCTCGGAGATCATCTCTTGAGA AACATAGAAAAGAAAAGCCTTTTAAAACCGGGAGAGGCAGAATTTCCACTCCTGAAAGAAAAGTAGCTAA AAAGGAACCTAGCACAGTCTCCAGAGATGAAGTGAGAAGGAAAAAAGCAGTTTATAAGAAGGCTGAACTT GCTAAAAAAACAGAAGTTCAGGCCCACTCTCCCTCCAGGAAATTCATTTTAAAACCTGCTATCAAATATA CTAGACCAACTCATCTCTCCTGTGTTAAGCGGAAAACCACAGCAGCAGGTGGGGAATCAGCTCTGGCTCC CAGTGTATTTAAACAGGCAAAGGACAAAGTCTCTGACGGAGTAACCAAGAGCCCAGAAAAGCGCTCTTCT CTCCCAAGACCTTCCTCCATTCTCCCTCCTCGGCGAGGTGTGTCAGGAGACAGAGATGAGAATTCCTTCT CTCTCAACAGTTCTATCTCTTCTTCAGCACGGCGGACCACCAGGTCAGAGCCAATTCGCAGAGCAGGGAA GAGTGGTACCTCAACACCCACTACCCCTGGGTCTACTGCCATCACTCCTGGCACCCCACCAAGTTATTCT TCACGCACACCAGGCACTCCTGGAACCCCTAGCTATCCCAGGACCCCTCACACACCAGGAACCCCCAAGT CTGCCATCTTGGTGCCGAGTGAGAAGAAGGTCGCCATCATACGTACTCCTCCAAAATCTCCTGCGACTCC CAAGCAGCTTCGGCTTATTAACCAACCACTGCCAGACCTGAAGAATGTCAAATCCAAAATCGGATCAACA GACAACATCAAATACCAGCCTAAAGGGGGGCAGGTACAAATTGTTACCAAGAAAATAGACCTAAGCCATG TGACATCCAAATGTGGCTCTCTGAAGAACATCGGCCACAGGCCAGGTGGCGGACGTGTGAAAATTGAGAG TGTAAAACTAGATTTCAAAGAAAAGGCCCAAGCTAAAGTTGGTTCTCTTGATAATGCTCATCATGTACCT GGAGGTGGTAATGTCAAGATTGACAGCCAAAAGTTGAACTTCAGAGAGCATGCTAAAGCCCGTGTGGACC ATGGGGCTGAGATCATTACACAGTCCCCAGGCAGATCCAGCGTGGCATCACCCCGACGACTCAGCAATGT CTCCTCGTCTGGAAGCATCAACCTGCTCGAATCTCCTCAGCTTGCCACTTTGGCTGAGGATGTCACTGCT GCACTCGCTAAGCAGGGCTTG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCTGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC216775 representing NM_002374 Red=Cloning site Green=Tags(s) MADERKDEAKAPHWTSAPLTEASAHSHPPEIKDQGGAGEGLVRSANGFPYREDEEGAFGEHGSQGTYSNT KENGINGELTSADRETAEEVSARIVQVVTAEAVAVLKGEQEKEAQHKDQTAALPLAAEETANLPPSPPPS PASEQTVTVEEDLLTASKMEFHDQQELTPSTAEPSDQKEKESEKQSKPGEDLKHAALVSQPETTKTYPDK KDMQGTEEEKAPLALFGHTLVASLEDMKQKTEPSLVVPGIDLPKEPPTPKEQKDWFIEMPTEAKKDEWGL VAPISPGPLTPMREKDVFDDIPKWEGKQFDSPMPSPFQGGSFTLPLDVMKNEIVTETSPFAPAFLQPDDK KSLQQTSGPATAKDSFKIEEPHEAKPDKMAEAPPSEAMTLPKDAHIPVVEEHVMGKVLEEEKEAINQETV QQRDTFTPSGQEPILTEKETELKLEEKTTISDKEAVPKESKPPKPADEEIGIIQTSTEHTFSEQKDQEPT TDMLKQDSFPVSLEQAVTDSAMTSKTLEKAMTEPSALIEKSSIQELFEMRVDDKDKIEGVGAATSAELDM PFYEDKSGMSKYFETSALKEEATKSIEPGSDYYELSDTRESVHESIDTMSPMHKNGDKEFQTGKESQPSP PAQEAGYSTLAQSYPSDLPEEPSSPQERMFTIDPKVYGEKRDLHSKNKDDLTLSRSLGLGGRSAIEQRSM SINLPMSCLDSIALGFNFGRGHDLSPLASDILTNTSGSMDEGDDYLPATTPALEKAPCFPVESKEEEQIE KVKATGEESTQAEISCESPFLAKDFYKNGTVMAPDLPEMLDLAGTRSRLASVSADAEVARRKSVPSETVV EDSRTGLPPVTDENHVIVKTDSQLEDLGYCVFNKYTVPLPSPVQDSENLSGESGTFYEGTDDKVRRDLAT DLSLIEVKLAAAGRVKDEFSVDKEASAHISGDKSGLSKEFDQEKKANDRLDTVLEKSEEHADSKEHAKKT EEAGDEIETFGLGVTYEQALAKDLSIPTDASSEKAEKGLSSVPEIAEVEPSKKVEQGLDFAVQGQLDVKI SDFGQMASGLNIDDRRATELKLEATQDMTPSSKAPQEADAFMGVESGHMKEGTKVSETEVKQKVAKPDLV HQEAVDKEESYESSGEHESLTMESLKADEGKKETSPESSLIQDEIAVKLSVEIPCPPAVSEADLATDERA DVQMEFIQGPKEESKETPDISITPSDVAEPLHETIVSEPAEIQSEEEEIEAQGEYDKLLFRSDTLQITDL GVSGAREEFVETCPSEHKGVIESVVTIEDDFITVVQTTTDEGESGSHSVRFAALEQPEVERRPSPHDEEE FEVEEAAEAQAEPKDGSPEAPASPEREEVALSEYKTETYDDYKDETTIDDSIMDADSLWVDTQDDDRSIM TEQLETIPKEEKAEKEARRSSLEKHRKEKPFKTGRGRISTPERKVAKKEPSTVSRDEVRRKKAVYKKAEL AKKTEVQAHSPSRKFILKPAIKYTRPTHLSCVKRKTTAAGGESALAPSVFKQAKDKVSDGVTKSPEKRSS LPRPSSILPPRRGVSGDRDENSFSLNSSISSSARRTTRSEPIRRAGKSGTSTPTTPGSTAITPGTPPSYS SRTPGTPGTPSYPRTPHTPGTPKSAILVPSEKKVAIIRTPPKSPATPKQLRLINQPLPDLKNVKSKIGST DNIKYQPKGGQVQIVTKKIDLSHVTSKCGSLKNIGHRPGGGRVKIESVKLDFKEKAQAKVGSLDNAHHVP GGGNVKIDSQKLNFREHAKARVDHGAEIITQSPGRSSVASPRRLSNVSSSGSINLLESPQLATLAEDVTA ALAKQGL TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_002374
ORF Size	5481 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_002374.2, NP_002365.2
RefSeq Size	6534 bp
RefSeq ORF	5484 bp
Locus ID	4133
UniProt ID	P11137
Domains	tubulin-binding
Protein Families	Adult stem cells, Druggable Genome, ES Cell Differentiation/IPS
MW	199.3 kDa
Gene Summary	This gene encodes a protein that belongs to the microtubule-associated protein family. The proteins of this family are thought to be involved in microtubule assembly, which is an essential step in neurogenesis. The products of similar genes in rat and mouse are neuron-specific cytoskeletal proteins that are enriched in dentrites, implicating a role in determining and stabilizing dentritic shape during neuron development. A number of alternatively spliced variants encoding distinct isoforms have been described. [provided by RefSeq, Jan 2010]

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