SALL3 (NM_171999) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	ZNF796
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC219967 representing NM_171999 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCTCGGCGCAAGCAGGCCAAGCCCCAGCACCTCAAGTCGGACGAGGAGCTGCTGCCGCCTGACGGGG CTCCCGAGCACGCCGCCCCGGGGGAAGGTGCGGAGGACGCAGACAGCGGGCCCGAGAGCCGCAGCGGGGG CGAGGAGACCAGCGTGTGCGAGAAATGCTGCGCCGAGTTCTTCAAGTGGGCGGACTTCCTGGAGCACCAG CGGAGCTGCACCAAGCTCCCGCCCGTGCTGATCGTGCACGAGGACGCGCCCGCGCCGCCCCACGAGGACT TCCCCGAGCCTTCGCCCGCCAGCTCCCCCAGCGAGCGCGCCGAAAGCGAGGCGGCCGAGGAGGCGGGTGC GGAGGGCGCGGAGGGCGAGGCCAGGCCGGTGGAGAAGGAGGCCGAGCCCATGGACGCGGAACCCGCGGGG GACACGCGCGCGCCCCGGCCCCCGCCTGCGGCCCCTGCACCCCCAACGCCCGCCTACGGCGCGCCCAGCA CCAACGTGACCCTGGAGGCGCTGCTGAGCACCAAGGTGGCGGTGGCGCAGTTCTCGCAGGGCGCGCGCGC GGCAGGCGGCTCGGGAGCAGGTGGAGGCGTGGCAGCTGCAGCCGTGCCCCTGATCCTGGAACAGCTCATG GCCCTGCAGCAGCAGCAGATCCACCAGCTGCAGCTCATCGAGCAGATCCGCAGCCAGGTGGCCCTCATGC AGCGCCCGCCGCCGCGGCCCTCACTCAGCCCCGCGGCCGCCCCGAGCGCACCGGGCCCGGCCCCCAGCCA GCTGCCCGGGCTGGCCGCGCTCCCGCTGTCGGCCGGGGCCCCTGCCGCCGCCATCGCGGGCTCGGGCCCC GCCGCCCCGGCCGCCTTCGAGGGCGCGCAGCCGCTGTCCCGGCCCGAGTCTGGCGCCAGCACCCCCGGCG GCCCTGCGGAGCCCAGCGCGCCCGCCGCCCCCAGCGCCGCCCCTGCCCCCGCTGCCCCCGCCCCGGCGCC AGCGCCGCAGAGCGCAGCCTCGTCGCAGCCGCAGAGCGCATCCACGCCGCCTGCCCTGGCCCCGGGGTCC CTGCTGGGTGCGGCGCCCGGCCTGCCAAGTCCGCTTCTACCTCAGACTTCCGCCAGCGGCGTCATCTTCC CCAACCCGCTGGTCAGCATCGCGGCCACGGCCAACGCTCTGGACCCGCTGTCCGCGCTCATGAAGCACCG CAAGGGCAAGCCGCCCAATGTGTCGGTGTTCGAGCCCAAAGCCAGCGCCGAGGACCCGTTCTTCAAGCAC AAATGCCGCTTCTGCGCCAAGGTCTTCGGCAGCGACAGCGCGCTCCAGATCCACCTGCGCTCGCACACAG GCGAGCGGCCCTTCAAGTGCAACATCTGCGGGAACCGCTTCTCCACCAAAGGCAACCTGAAGGTGCACTT CCAGAGGCACAAGGAGAAGTACCCCCACATCCAGATGAACCCTTACCCGGTCCCCGAGTACCTGGACAAC GTGCCCACCTGCTCGGGCATCCCCTACGGCATGTCGCTGCCCCCCGAGAAGCCCGTGACCACCTGGCTGG ACAGCAAGCCCGTGCTGCCCACCGTGCCCACGTCCGTGGGGCTGCAACTGCCGCCCACTGTCCCTGGCGC GCACGGCTACGCCGACTCTCCCAGCGCCACCCCAGCCAGCCGCTCCCCGCAGAGGCCCTCGCCCGCCTCC AGCGAGTGCGCCTCCTTGTCCCCAGGCCTCAACCACGTGGAGTCCGGCGTGTCGGCCACCGCCGAGTCCC CACAGTCGCTCCTCGGCGGGCCGCCCGTCACTAAAGCCGAGCCCGTCAGCCTGCCCTGCACCAACGCCAG GGCCGGGGACGCTCCCGTGGGCGCGCAGGCTAGCGCTGCACCCACATCGGTGGACGGCGCACCCACGAGC CTCGGCAGCCCCGGGCTGCCCGCCGTCTCCGAGCAGTTCAAGGCCCAGTTTCCGTTCGGGGGGCTGCTAG ACTCGATGCAAACGTCGGAAACCTCGAAGCTGCAGCAGCTGGTGGAGAACATCGACAAGAAGATGACGGA CCCGAACCAGTGCGTCATCTGCCACCGGGTGCTGAGCTGCCAGAGCGCGCTGAAGATGCACTACCGGACG CACACGGGGGAGCGGCCGTTCAAGTGCAAGATCTGCGGCCGCGCCTTCACCACCAAGGGCAACCTCAAGA CGCACTTCGGCGTGCACCGTGCAAAGCCGCCCCTGCGCGTGCAGCACTCCTGCCCCATCTGCCAGAAGAA GTTCACCAACGCCGTGGTCCTGCAGCAGCACATCCGCATGCACATGGGCGGCCAGATCCCCAACACGCCG CTGCCGGAGGGCTTCCAGGATGCCATGGACTCCGAGCTGGCCTACGACGACAAGAACGCGGAGACCCTGA GCAGCTACGATGACGACATGGACGAGAACTCCATGGAGGACGACGCTGAGCTGAAGGACGCGGCCACCGA CCCGGCCAAGCCACTCCTGTCCTATGCGGGGTCCTGCCCGCCCTCCCCGCCCTCGGTCATCTCCAGCATT GCCGCCCTGGAGAACCAGATGAAGATGATCGACTCGGTCATGAGCTGCCAGCAGCTGACCGGCCTCAAGT CCGTGGAGAACGGGTCCGGGGAGAGTGACCGCCTGAGCAACGACTCCTCGTCGGCCGTGGGCGACCTGGA GAGCCGCAGCGCGGGCAGCCCCGCCCTGTCCGAGTCCTCGTCCTCGCAGGCCCTGTCGCCGGCCCCCAGC AATGGTGAGAGCTTCCGCTCCAAGTCCCCGGGCCTGGGCGCCCCGGAGGAGCCCCAGGAAATCCCGCTCA AGACCGAGAGGCCGGACAGCCCAGCCGCCGCCCCGGGCAGCGGAGGCGCCCCTGGCCGCGCGGGCATCAA GGAGGAGGCGCCCTTCAGCCTGCTGTTCCTGAGCAGGGAGCGGGGTAAGTGTCCCAGCACTGTGTGTGGT GTCTGTGGCAAGCCTTTTGCTTGCAAGAGCGCGTTGGAAATCCACTACCGCAGCCATACTAAGGAGCGGC CATTCGTCTGCGCGCTCTGCAGGCGAGGGTGCTCCACTATGGGTAATTTAAAACAGCACTTACTGACACA CAGATTGAAAGAGCTGCCTTCTCAGTTATTTGACCCCAACTTTGCTCTAGGTCCCAGCCAAAGCACTCCT AGCCTGATCTCCAGCGCCGCACCCACCATGATCAAAATGGAAGTGAACGGTCACGGCAAGGCCATGGCGC TGGGCGAGGGTCCCCCGCTGCCCGCGGGCGTCCAGGTCCCCGCCGGGCCTCAGACAGTGATGGGCCCGGG CCTGGCGCCCATGCTGGCCCCCCCACCGCGCCGGACGCCCAAGCAGCACAACTGCCAGTCGTGCGGGAAG ACCTTCTCCTCGGCCAGCGCCCTGCAGATCCATGAGCGCACGCACACCGGCGAGAAGCCGTTCGGCTGCA CCATCTGCGGCCGGGCCTTCACCACTAAGGGCAACCTCAAGGTGCACATGGGGACACACATGTGGAATAA CGCCCCCGCGAGACGCGGCCGCCGCCTGTCTGTGGAGAACCCCATGGCTCTCCTAGGGGGTGATGCCCTG AAGTTCTCTGAAATGTTCCAGAAGGACCTGGCAGCTCGGGCAATGAACGTCGACCCCAGTTTTTGGAACC AGTATGCTGCAGCCATCACTAACGGGCTCGCCATGAAGAACAACGAGATCTCCGTCATCCAGAACGGCGG CATCCCCCAGCTCCCCGTGAGTCTTGGGGGCAGCGCCCTCCCCCCTCTGGGCAGCATGGCCAGTGGGATG GACAAAGCACGCACTGGCAGTAGCCCACCCATCGTCAGCTTGGACAAAGCGAGCTCAGAAACAGCAGCCA GCCGCCCATTCACGCGGTTTATCGAGGATAACAAGGAGATTGGTATCAAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC219967 representing NM_171999 Red=Cloning site Green=Tags(s) MSRRKQAKPQHLKSDEELLPPDGAPEHAAPGEGAEDADSGPESRSGGEETSVCEKCCAEFFKWADFLEHQ RSCTKLPPVLIVHEDAPAPPHEDFPEPSPASSPSERAESEAAEEAGAEGAEGEARPVEKEAEPMDAEPAG DTRAPRPPPAAPAPPTPAYGAPSTNVTLEALLSTKVAVAQFSQGARAAGGSGAGGGVAAAAVPLILEQLM ALQQQQIHQLQLIEQIRSQVALMQRPPPRPSLSPAAAPSAPGPAPSQLPGLAALPLSAGAPAAAIAGSGP AAPAAFEGAQPLSRPESGASTPGGPAEPSAPAAPSAAPAPAAPAPAPAPQSAASSQPQSASTPPALAPGS LLGAAPGLPSPLLPQTSASGVIFPNPLVSIAATANALDPLSALMKHRKGKPPNVSVFEPKASAEDPFFKH KCRFCAKVFGSDSALQIHLRSHTGERPFKCNICGNRFSTKGNLKVHFQRHKEKYPHIQMNPYPVPEYLDN VPTCSGIPYGMSLPPEKPVTTWLDSKPVLPTVPTSVGLQLPPTVPGAHGYADSPSATPASRSPQRPSPAS SECASLSPGLNHVESGVSATAESPQSLLGGPPVTKAEPVSLPCTNARAGDAPVGAQASAAPTSVDGAPTS LGSPGLPAVSEQFKAQFPFGGLLDSMQTSETSKLQQLVENIDKKMTDPNQCVICHRVLSCQSALKMHYRT HTGERPFKCKICGRAFTTKGNLKTHFGVHRAKPPLRVQHSCPICQKKFTNAVVLQQHIRMHMGGQIPNTP LPEGFQDAMDSELAYDDKNAETLSSYDDDMDENSMEDDAELKDAATDPAKPLLSYAGSCPPSPPSVISSI AALENQMKMIDSVMSCQQLTGLKSVENGSGESDRLSNDSSSAVGDLESRSAGSPALSESSSSQALSPAPS NGESFRSKSPGLGAPEEPQEIPLKTERPDSPAAAPGSGGAPGRAGIKEEAPFSLLFLSRERGKCPSTVCG VCGKPFACKSALEIHYRSHTKERPFVCALCRRGCSTMGNLKQHLLTHRLKELPSQLFDPNFALGPSQSTP SLISSAAPTMIKMEVNGHGKAMALGEGPPLPAGVQVPAGPQTVMGPGLAPMLAPPPRRTPKQHNCQSCGK TFSSASALQIHERTHTGEKPFGCTICGRAFTTKGNLKVHMGTHMWNNAPARRGRRLSVENPMALLGGDAL KFSEMFQKDLAARAMNVDPSFWNQYAAAITNGLAMKNNEISVIQNGGIPQLPVSLGGSALPPLGSMASGM DKARTGSSPPIVSLDKASSETAASRPFTRFIEDNKEIGIN TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms	CHROMATOGRAMS Sequencher program is needed, download here.
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_171999
ORF Size	3900 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_171999.1, NP_741996.1
RefSeq Size	4772 bp
RefSeq ORF	3903 bp
Locus ID	27164
UniProt ID	Q9BXA9
MW	135.2 kDa
Gene Summary	This gene encodes a sal-like C2H2-type zinc-finger protein, and belongs to a family of evolutionarily conserved genes found in species as diverse as Drosophila, C. elegans, and vertebrates. Mutations in some of these genes are associated with congenital disorders in human, suggesting their importance in embryonic development. This protein binds to DNA methyltransferase 3 alpha (DNMT3A), and reduces DNMT3A-mediated CpG island methylation. It is suggested that silencing of this gene, resulting in acceleration of DNA methylation, may have a role in oncogenesis. [provided by RefSeq, Oct 2011]

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