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EAAT4 (SLC1A6) (NM_005071) Human Tagged ORF Clone
CAT＃: RC221087
SLC1A6 (Myc-DDK-tagged)-Human solute carrier family 1 (high affinity aspartate/glutamate transporter), member 6 (SLC1A6)
ORF Plasmid: tGFP
"NM_005071" in other vectors (4)
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CNY 4,200.00
CNY 4,560.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | EAAT4 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC221087 representing NM_005071
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGCAGCCATGGCAACAGCCTGTTCCTTCGGGAGAGCGGCCAGCGGCTGGGCCGGGTGGGCTGGCTGC AGCGGCTGCAGGAAAGCCTGCAGCAGAGAGCACTGCGCACGCGCCTGCGCCTGCAGACCATGACCCTCGA GCACGTGCTGCGCTTCCTGCGCCGAAACGCCTTCATTCTGCTGACGGTCAGCGCCGTGGTCATTGGGGTC AGCCTGGCCTTTGCCCTGCGCCCATATCAGCTCACCTACCGCCAGATCAAGTACTTCTCTTTTCCTGGAG AGCTTCTGATGAGGATGCTGCAGATGCTGGTGTTACCTCTCATTGTCTCCAGCCTGGTCACAGGTATGGC ATCCCTGGACAACAAGGCCACGGGGCGGATGGGGATGCGGGCAGCTGTGTACTACATGGTGACCACCATC ATCGCGGTCTTCATCGGCATCCTCATGGTCACCATCATCCATCCCGGGAAGGGCTCCAAGGAGGGGCTGC ACCGGGAGGGCCGGATCGAGACCATCCCCACAGCTGATGCCTTCATGGACCTGATCAGAAATATGTTTCC ACCAAACCTTGTGGAGGCCTGCTTCAAACAGTTCAAGACGCAGTACAGCACGAGGGTGGTAACCAGGACC ATGGTGAGGACAGAGAACGGGTCTGAGCCGGGTGCCTCCATGCCTCCTCCATTCTCAGTGGAGAACGGAA CCAGCTTCCTGGAAAATGTCACTCGGGCCTTGGGTACCCTGCAGGAGATGCTGAGCTTTGAGGAGACTGT ACCCGTGCCTGGCTCCGCCAATGGCATCAACGCCCTGGGCCTCGTGGTCTTCTCTGTGGCCTTTGGGCTG GTCATTGGTGGCATGAAACACAAGGGCAGAGTCCTCAGGGACTTCTTCGACAGCCTCAATGAGGCTATTA TGAGGCTGGTGGGCATCATTATCTGGTATGCACCTGTGGGCATCCTGTTCCTGATTGCTGGGAAGATTCT GGAGATGGAAGACATGGCCGTCCTGGGGGGTCAGCTGGGCATGTACACCCTGACCGTCATCGTGGGCCTG TTCCTCCATGCCGGCATTGTCCTTCCCCTCATCTACTTCCTCGTCACTCACCGGAACCCCTTCCCCTTCA TTGGGGGCATGCTACAAGCCCTCATCACCGCTATGGGCACGTCTTCCAGCTCGGCAACGCTGCCCATCAC CTTCCGCTGCCTGGAGGAGGGCCTGGGTGTGGACCGCCGCATCACCAGGTTCGTCCTGCCCGTGGGCGCC ACGGTCAACATGGATGGCACTGCCCTCTACGAGGCCCTGGCTGCCATCTTCATTGCTCAAGTTAACAACT ACGAGCTCAACCTGGGTCAGATCACAACCATCAGCATCACGGCCACAGCAGCCAGTGTTGGGGCTGCTGG CATCCCCCAGGCGGGTCTGGTCACCATGGTCATTGTGCTTACGTCGGTCGGCTTGCCCACGGAAGACATC ACGCTCATCATTGCCGTGGACTGGTTCCTTGACCGGCTTCGCACAATGACCAACGTACTGGGGGACTCAA TTGGAGCGGCCGTCATCGAGCACTTGTCTCAGCGGGAGCTGGAGCTTCAGGAAGCTGAGCTTACCCTCCC CAGCCTGGGGAAACCCTACAAGTCCCTCATGGCACAGGAGAAGGGGGCATCCCGGGGACGGGGAGGCAAC GAGAGTGCTATG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC221087 representing NM_005071
Red=Cloning site Green=Tags(s) MSSHGNSLFLRESGQRLGRVGWLQRLQESLQQRALRTRLRLQTMTLEHVLRFLRRNAFILLTVSAVVIGV SLAFALRPYQLTYRQIKYFSFPGELLMRMLQMLVLPLIVSSLVTGMASLDNKATGRMGMRAAVYYMVTTI IAVFIGILMVTIIHPGKGSKEGLHREGRIETIPTADAFMDLIRNMFPPNLVEACFKQFKTQYSTRVVTRT MVRTENGSEPGASMPPPFSVENGTSFLENVTRALGTLQEMLSFEETVPVPGSANGINALGLVVFSVAFGL VIGGMKHKGRVLRDFFDSLNEAIMRLVGIIIWYAPVGILFLIAGKILEMEDMAVLGGQLGMYTLTVIVGL FLHAGIVLPLIYFLVTHRNPFPFIGGMLQALITAMGTSSSSATLPITFRCLEEGLGVDRRITRFVLPVGA TVNMDGTALYEALAAIFIAQVNNYELNLGQITTISITATAASVGAAGIPQAGLVTMVIVLTSVGLPTEDI TLIIAVDWFLDRLRTMTNVLGDSIGAAVIEHLSQRELELQEAELTLPSLGKPYKSLMAQEKGASRGRGGN ESAM TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_005071 |
| ORF Size | 1692 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_005071.2 |
| RefSeq Size | 1719 bp |
| RefSeq ORF | 1695 bp |
| Locus ID | 6511 |
| UniProt ID | P48664 |
| Domains | SDF |
| Protein Families | Transmembrane |
| MW | 61.4 kDa |
| Gene Summary | Sodium-dependent, high-affinity amino acid transporter that mediates the uptake of L-glutamate and also L-aspartate and D-aspartate (PubMed:7791878). Functions as a symporter that transports one amino acid molecule together with two or three Na(+) ions and one proton, in parallel with the counter-transport of one K(+) ion. Mediates Cl(-) flux that is not coupled to amino acid transport; this avoids the accumulation of negative charges due to aspartate and Na(+) symport (By similarity). Plays a redundant role in the rapid removal of released glutamate from the synaptic cleft, which is essential for terminating the postsynaptic action of glutamate (Probable).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC221087L3 | Lenti ORF clone of Human solute carrier family 1 (high affinity aspartate/glutamate transporter), member 6 (SLC1A6), Myc-DDK-tagged |
CNY 6,460.00 |
|
| RC221087L4 | Lenti ORF clone of Human solute carrier family 1 (high affinity aspartate/glutamate transporter), member 6 (SLC1A6), mGFP tagged |
CNY 6,460.00 |
|
| RG221087 | SLC1A6 (tGFP-tagged) - Human solute carrier family 1 (high affinity aspartate/glutamate transporter), member 6 (SLC1A6) |
CNY 5,040.00 |
|
| SC125329 | SLC1A6 (untagged)-Human solute carrier family 1 (high affinity aspartate/glutamate transporter), member 6 (SLC1A6) |
CNY 4,208.00 |
