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IREB2 (NM_004136) Human Tagged ORF Clone
CAT＃: RC221385
IREB2 (Myc-DDK-tagged)-Human iron-responsive element binding protein 2 (IREB2)
ORF Plasmid: tGFP
"NM_004136" in other vectors (6)
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CNY 7,056.00
CNY 7,130.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | ACO3; IRE-BP 2; IRE-BP2; IRP2; IRP2AD; NDCAMA |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC221385 representing NM_004136
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGACGCCCCAAAAGCAGGATACGCCTTTGAGTACCTTATTGAAACATTAAATGACAGTTCACATAAGA AGTTCTTCGATGTATCTAAACTTGGCACCAAGTATGATGTTCTGCCTTACTCAATACGGGTCTTGTTGGA AGCTGCTGTACGAAATTGTGATGGCTTTTTAATGAAGAAGGAAGATGTTATGAACATTTTAGACTGGAAA ACCAAACAAAGCAATGTTGAAGTGCCCTTTTTCCCTGCCCGTGTTCTTCTTCAAGATTTTACTGGAATAC CAGCAATGGTGGATTTTGCTGCTATGAGGGAGGCAGTGAAAACTCTTGGAGGTGATCCTGAGAAAGTCCA TCCTGCTTGTCCGACAGATCTTACAGTTGACCATTCTTTACAAATTGACTTCAGTAAATGTGCAATACAG AATGCACCAAATCCTGGAGGTGGTGACCTGCAGAAAGCAGGAAAGCTCTCTCCACTTAAAGTGCAGCCTA AGAAGCTTCCCTGCAGAGGCCAGACTACCTGCCGAGGATCTTGTGATTCTGGAGAACTAGGCCGAAACTC AGGAACATTTTCTTCGCAGATTGAGAATACACCCATCCTGTGTCCTTTTCATTTGCAACCAGTGCCTGAA CCTGAAACAGTGTTAAAAAATCAAGAAGTAGAATTCGGCAGAAATCGAGAGAGGCTTCAGTTTTTTAAGT GGAGTTCAAGAGTTTTTAAGAATGTGGCAGTGATCCCTCCTGGAACTGGAATGGCTCATCAAATAAACTT AGAATATTTGTCAAGAGTGGTTTTTGAAGAAAAAGACCTCCTCTTCCCAGACAGTGTAGTCGGCACAGAT TCACACATAACGATGGTGAATGGTTTAGGGATTCTGGGGTGGGGGGTTGGAGGCATTGAAACAGAAGCAG TTATGCTTGGTCTGCCAGTTTCTCTTACTTTACCAGAGGTGGTTGGATGTGAGTTAACTGGGTCATCAAA CCCTTTTGTTACATCCATAGATGTTGTTCTTGGTATTACAAAGCACCTCAGGCAAGTAGGAGTGGCTGGA AAGTTTGTTGAGTTTTTTGGAAGTGGAGTTTCACAATTATCTATAGTTGATCGAACTACAATAGCAAACA TGTGTCCGGAATATGGTGCTATCCTCAGCTTTTTCCCTGTTGACAATGTGACATTAAAACATTTAGAACA TACAGGTTTTAGCAAAGCCAAACTCGAATCAATGGAAACATACCTTAAAGCTGTGAAATTGTTTCGAAAT GACCAGAATTCTTCAGGAGAACCTGAATACTCCCAGGTGATCCAGATTAATCTGAATTCAATAGTTCCAT CTGTTAGTGGTCCAAAAAGACCTCAGGATAGAGTTGCTGTGACAGATATGAAAAGCGATTTCCAGGCTTG CTTAAATGAAAAGGTTGGATTTAAAGGCTTCCAAATTGCAGCTGAAAAACAAAAGGATATTGTCTCCATT CATTATGAAGGAAGTGAATATAAGCTGTCTCATGGATCAGTGGTCATTGCTGCAGTTATCAGTTGTACCA ATAATTGCAATCCATCTGTCATGCTTGCTGCAGGTCTTTTGGCTAAAAAGGCTGTTGAAGCTGGTCTGCG TGTTAAACCTTATATAAGAACAAGTTTATCTCCAGGCAGTGGGATGGTTACACATTACCTCAGTTCAAGT GGAGTATTACCATATCTAAGTAAGCTTGGATTTGAAATCGTTGGCTATGGATGTTCAACTTGTGTGGGAA ATACAGCACCCTTATCAGACGCAGTTTTAAATGCAGTAAAACAGGGTGATTTGGTTACCTGTGGAATTTT ATCTGGAAACAAAAATTTTGAAGGTCGTCTTTGTGATTGTGTTCGTGCCAATTATCTTGCCTCTCCACCC TTAGTGGTAGCTTATGCCATAGCAGGCACAGTGAATATAGATTTCCAGACAGAACCTTTAGGTACTGACC CCACCGGCAAGAACATTTACCTGCATGATATTTGGCCTAGTCGAGAAGAAGTTCATCGAGTAGAGGAAGA ACATGTTATACTATCCATGTTTAAAGCATTAAAAGATAAAATAGAAATGGGGAATAAACGGTGGAATTCC TTAGAAGCACCGGATTCAGTTTTGTTTCCATGGGACTTAAAGTCTACTTATATCAGATGCCCTTCATTTT TTGATAAACTTACCAAAGAGCCAATTGCACTCCAGGCTATTGAAAATGCCCATGTCTTATTATATTTGGG AGACTCTGTCACAACAGATCATATATCACCTGCAGGAAGTATCGCTAGGAATAGTGCTGCCGCTAAGTAT TTGACAAACAGAGGCCTTACCCCTCGTGAATTCAACTCTTACGGAGCTCGAAGAGGTAATGATGCTGTAA TGACAAGAGGCACTTTTGCAAATATCAAGCTTTTTAATAAGTTTATTGGAAAACCAGCTCCTAAAACAAT TCATTTTCCATCAGGACAGACGCTAGATGTATTTGAGGCTGCAGAGCTGTACCAGAAAGAAGGTATCCCA CTGATTATTTTAGCAGGAAAGAAATATGGTTCAGGAAACTCCAGAGACTGGGCTGCCAAAGGACCGTATT TACTGGGTGTGAAAGCTGTTTTGGCTGAAAGTTATGAAAAAATACACAAAGATCATTTGATTGGAATTGG CATAGCTCCACTTCAGTTCCTTCCAGGAGAAAATGCAGATTCCTTGGGCCTCTCCGGTAGAGAAACATTT TCTTTAACATTTCCTGAAGAACTGTCTCCTGGAATTACATTGAATATACAGACAAGCACTGGAAAAGTAT TCAGCGTGATTGCTTCGTTTGAAGATGATGTGGAAATAACATTATACAAACATGGAGGATTATTAAACTT TGTGGCACGAAAATTCTCA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC221385 representing NM_004136
Red=Cloning site Green=Tags(s) MDAPKAGYAFEYLIETLNDSSHKKFFDVSKLGTKYDVLPYSIRVLLEAAVRNCDGFLMKKEDVMNILDWK TKQSNVEVPFFPARVLLQDFTGIPAMVDFAAMREAVKTLGGDPEKVHPACPTDLTVDHSLQIDFSKCAIQ NAPNPGGGDLQKAGKLSPLKVQPKKLPCRGQTTCRGSCDSGELGRNSGTFSSQIENTPILCPFHLQPVPE PETVLKNQEVEFGRNRERLQFFKWSSRVFKNVAVIPPGTGMAHQINLEYLSRVVFEEKDLLFPDSVVGTD SHITMVNGLGILGWGVGGIETEAVMLGLPVSLTLPEVVGCELTGSSNPFVTSIDVVLGITKHLRQVGVAG KFVEFFGSGVSQLSIVDRTTIANMCPEYGAILSFFPVDNVTLKHLEHTGFSKAKLESMETYLKAVKLFRN DQNSSGEPEYSQVIQINLNSIVPSVSGPKRPQDRVAVTDMKSDFQACLNEKVGFKGFQIAAEKQKDIVSI HYEGSEYKLSHGSVVIAAVISCTNNCNPSVMLAAGLLAKKAVEAGLRVKPYIRTSLSPGSGMVTHYLSSS GVLPYLSKLGFEIVGYGCSTCVGNTAPLSDAVLNAVKQGDLVTCGILSGNKNFEGRLCDCVRANYLASPP LVVAYAIAGTVNIDFQTEPLGTDPTGKNIYLHDIWPSREEVHRVEEEHVILSMFKALKDKIEMGNKRWNS LEAPDSVLFPWDLKSTYIRCPSFFDKLTKEPIALQAIENAHVLLYLGDSVTTDHISPAGSIARNSAAAKY LTNRGLTPREFNSYGARRGNDAVMTRGTFANIKLFNKFIGKPAPKTIHFPSGQTLDVFEAAELYQKEGIP LIILAGKKYGSGNSRDWAAKGPYLLGVKAVLAESYEKIHKDHLIGIGIAPLQFLPGENADSLGLSGRETF SLTFPEELSPGITLNIQTSTGKVFSVIASFEDDVEITLYKHGGLLNFVARKFS TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_004136 |
| ORF Size | 2889 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_004136.4 |
| RefSeq Size | 3928 bp |
| RefSeq ORF | 2892 bp |
| Locus ID | 3658 |
| UniProt ID | P48200 |
| Protein Families | Druggable Genome |
| MW | 104.9 kDa |
| Gene Summary | The protein encoded by this gene is an RNA-binding protein that acts to regulate iron levels in the cells by regulating the translation and stability of mRNAs that affect iron homeostasis under conditions when iron is depleted. When iron levels are low, this protein binds to iron-responsive elements (IRES), stem-loop structures located either in the 5' or 3' UTRs. Binding to the 5' UTR represses translation, while binding to the 3' UTR inhibits mRNA degradation. When iron is found in the cell, this protein is degraded in a F-box and leucine rich repeat protein 5-dependent manner. Variants in this gene have been associated with lung cancer and chronic obstructive pulmonary disease (COPD). Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Aug 2017] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC221385L1 | Lenti ORF clone of Human iron-responsive element binding protein 2 (IREB2), Myc-DDK-tagged |
CNY 9,030.00 |
|
| RC221385L2 | Lenti ORF clone of Human iron-responsive element binding protein 2 (IREB2), mGFP tagged |
CNY 9,030.00 |
|
| RC221385L3 | Lenti ORF clone of Human iron-responsive element binding protein 2 (IREB2), Myc-DDK-tagged |
CNY 9,030.00 |
|
| RC221385L4 | Lenti ORF clone of Human iron-responsive element binding protein 2 (IREB2), mGFP tagged |
CNY 9,030.00 |
|
| RG221385 | IREB2 (tGFP-tagged) - Human iron-responsive element binding protein 2 (IREB2) |
CNY 7,890.00 |
|
| SC128101 | IREB2 (untagged)-Human iron-responsive element binding protein 2 (IREB2) |
CNY 7,064.00 |
