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WHSC1 (NM_133331) Human Tagged ORF Clone
CAT＃: RC221401
- TrueORF®
WHSC1 (Myc-DDK-tagged)-Human Wolf-Hirschhorn syndrome candidate 1 (WHSC1), transcript variant 2
ORF Plasmid: tGFP
"NM_133331" in other vectors (4)
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CNY 14,944.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | KMT3F; KMT3G; MMSET; REIIBP; TRX5; WHS; WHSC1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC221401 representing NM_133331
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAATTTAGCATCAAGCAGAGTCCCCTTTCTGTTCAGAGTGTTGTAAAGTGCATAAAGATGAAGCAGG CACCAGAAATCCTCGGCAGTGCCAACGGGAAGACTCCGAGCTGCGAGGTGAACCGCGAGTGTTCTGTGTT CCTCAGCAAAGCCCAGCTCTCCAGTAGCCTGCAGGAGGGGGTCATGCAGAAGTTTAACGGCCACGACGCC CTGCCCTTTATTCCAGCCGACAAGCTGAAAGATCTTACTTCCCGGGTGTTTAATGGAGAACCCGGCGCAC ACGATGCCAAACTGCGTTTTGAGTCCCAGGAAATGAAAGGGATTGGGACACCCCCTAACACTACCCCTAT CAAAAATGGCTCTCCAGAAATTAAGCTGAAAATCACCAAAACATACATGAATGGGAAGCCTCTCTTTGAA TCTTCCATTTGTGGTGACAGTGCTGCTGATGTGTCTCAGTCAGAAGAAAATGGACAAAAACCAGAAAACA AGGCGAGAAGGAACAGGAAGAGGAGCATAAAATATGACTCCTTGCTGGAGCAGGGCCTTGTCGAAGCAGC TCTTGTGTCTAAGATCTCAAGTCCTTCAGATAAAAAGATTCCAGCTAAGAAAGAGTCTTGTCCAAACACT GGAAGAGACAAAGACCACCTGTTGAAATACAACGTTGGTGATTTGGTGTGGTCCAAAGTGTCGGGTTACC CTTGGTGGCCTTGCATGGTTTCTGCAGATCCACTCCTTCACAGCTATACCAAACTTAAAGGTCAGAAAAA GAGTGCACGCCAGTATCACGTACAGTTCTTTGGTGACGCCCCAGAAAGAGCTTGGATATTTGAGAAGAGC CTCGTAGCTTTTGAAGGAGAAGGACAGTTTGAAAAATTATGCCAGGAAAGTGCCAAGCAGGCACCCACGA AAGCTGAGAAAATTAAGCTATTGAAACCAATTTCAGGGAAATTGAGGGCCCAGTGGGAAATGGGCATTGT TCAAGCAGAAGAAGCTGCAAGCATGTCAGTGGAGGAGCGGAAAGCCAAGTTCACCTTTCTCTATGTGGGG GACCAGCTTCATCTCAACCCTCAAGTAGCCAAGGAGGCTGGCATTGCTGCAGAGTCTTTGGGAGAAATGG CAGAATCCTCAGGAGTCAGTGAAGAAGCTGCTGAAAACCCCAAGTCTGTGAGAGAAGAGTGCATTCCCAT GAAGAGAAGGCGGAGGGCCAAACTGTGTAGCTCTGCAGAGACCCTGGAGAGTCACCCCGACATAGGGAAG AGTACTCCTCAAAAGACGGCAGAGGCTGACCCCAGAAGAGGAGTAGGGTCTCCTCCTGGGAGGAAGAAGA CCACAGTCTCCATGCCACGAAGCAGGAAGGGAGATGCAGCATCCCAGTTTTTGGTCTTCTGTCAAAAACA CAGGGATGAGGTGGTAGCTGAGCACCCAGATGCTTCAGGTGAGGAGATTGAAGAGCTGCTCAGGTCACAG TGGAGTCTGCTGAGTGAGAAGCAGAGAGCACGCTACAACACCAAGTTTGCCCTGGTGGCCCCTGTCCAGG CTGAAGAAGACTCTGGTAATGTAAATGGGAAAAAAAGAAACCACACAAAGAGGATACAGGACCCTACAGA AGATGCTGAAGCTGAGGACACACCCAGGAAAAGACTCAGGACGGACAAGCACAGTCTTCGGAAGAGAGAC ACAATCACTGACAAAACGGCCAGAACAAGCTCTTACAAGGCCATGGAGGCAGCCTCCTCGCTCAAGAGCC AGGCAGCAACGAAAAATCTGTCTGATGCATGTAAACCACTGAAGAAGCGAAATCGGGCTTCCACGGCAGC ATCTTCAGCTCTTGGGTTTAGCAAAAGTTCATCTCCTTCTGCATCCTTAACTGAGAATGAGGTCTCGGAC AGCCCGGGAGACGAGCCCTCGGAGTCCCCATACGAAAGTGCAGACGAAACACAAACTGAAGTATCTGTCT CATCCAAAAAGTCTGAGCGAGGAGTGACTGCCAAAAAGGAGTATGTGTGCCAGCTGTGTGAGAAGCCGGG CAGCCTCCTGCTCTGTGAAGGACCCTGCTGCGGAGCTTTCCACCTCGCCTGCCTTGGGCTTTCCCGGAGG CCAGAAGGGAGGTTCACCTGCAGCGAGTGTGCCTCAGGGATTCACTCATGTTTCGTGTGTAAAGAGAGCA AGACAGATGTTAAGCGCTGTGTGGTAACTCAGTGTGGAAAATTTTACCATGAGGCTTGTGTGAAAAAATA CCCTCTGACTGTATTTGAGAGCCGAGGTTTCCGCTGCCCCCTCCACAGCTGTGTGAGCTGCCATGCTTCC AACCCTTCAAACCCAAGGCCGTCAAAAGGTAAAATGATGCGGTGTGTCCGCTGCCCCGTTGCCTATCACA GCGGGGATGCTTGTCTGGCAGCAGGATGCTCAGTGATCGCCTCCAACAGCATCATCTGCACTGCCCACTT CACTGCTCGGAAGGGGAAGCGACACCACGCCCACGTCAACGTGAGCTGGTGCTTCGTGTGCTCCAAAGGG GGGAGCCTTCTGTGCTGTGAGTCCTGCCCAGCGGCCTTCCACCCTGACTGCCTGAACATCGAGATGCCTG ACGGCAGCTGGTTCTGCAATGACTGCAGGGCTGGGAAGAAGCTGCACTTCCAGGATATCATTTGGGTGAA ACTTGGGAACTACAGATGGTGGCCGGCAGAAGTTTGCCATCCCAAAAATGTTCCCCCAAATATTCAGAAA ATGAAGCACGAGATTGGAGAATTCCCTGTGTTTTTCTTTGGGTCTAAAGATTATTACTGGACGCATCAGG CGCGAGTGTTCCCGTACATGGAGGGGGACCGGGGCAGCCGCTACCAGGGGGTCAGAGGGATCGGAAGAGT CTTCAAAAACGCACTGCAAGAAGCTGAAGCTCGTTTTCGTGAAATTAAGCTTCAGAGGGAAGCCCGAGAA ACACAGGAGAGCGAGCGCAAGCCCCCACCATACAAGCACATCAAGGTGAATAAGCCTTACGGGAAAGTCC AGATCTACACAGCGGATATTTCAGAAATCCCTAAGTGCAACTGCAAGCCCACAGATGAGAATCCTTGTGG CTTTGATTCGGAGTGTCTGAACAGGATGCTGATGTTTGAGTGCCACCCGCAGGTGTGTCCCGCGGGCGAG TTCTGCCAGAACCAGTGCTTCACCAAGCGCCAGTACCCAGAGACCAAGATCATCAAGACAGATGGCAAAG GGTGGGGCCTGGTCGCCAAGAGGGACATCAGAAAGGGAGAATTTGTTAACGAGTACGTTGGGGAGCTGAT CGACGAGGAGGAGTGCATGGCGAGAATCAAGCACGCACACGAGAACGACATCACCCACTTCTACATGCTC ACTATAGACAAGGACCGTATAATAGACGCTGGCCCCAAAGGAAACTACTCTCGATTTATGAATCACAGCT GCCAGCCCAACTGTGAGACCCTCAAGTGGACAGTGAATGGGGACACTCGTGTGGGCCTGTTTGCCGTCTG TGACATTCCTGCAGGGACGGAGCTGACTTTTAACTACAACCTCGATTGTCTGGGCAATGAAAAAACGGTC TGCCGGTGTGGAGCCTCCAATTGCAGTGGATTCCTCGGGGATAGACCAAAGACCTCGACGACCCTTTCAT CAGAGGAAAAGGGCAAAAAGACCAAGAAGAAAACGAGGCGGCGCAGAGCAAAAGGGGAAGGGAAGAGGCA GTCAGAGGACGAGTGCTTCCGCTGCGGTGATGGCGGGCAGCTGGTGCTGTGTGACCGCAAGTTCTGCACC AAGGCCTACCACCTGTCCTGCCTGGGCCTTGGCAAGCGGCCCTTCGGGAAGTGGGAATGTCCTTGGCATC ATTGTGACGTGTGTGGCAAACCTTCGACTTCATTTTGCCACCTCTGCCCCAATTCGTTCTGTAAGGAGCA CCAGGACGGGACAGCCTTCAGCTGCACCCCGGACGGGCGGTCCTACTGCTGTGAGCATGACTTAGGGGCG GCATCGGTCAGAAGCACCAAGACTGAGAAGCCCCCCCCAGAGCCAGGGAAGCCGAAGGGGAAGAGGCGGC GGCGGAGGGGCTGGCGGAGAGTCACAGAGGGCAAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC221401 representing NM_133331
Red=Cloning site Green=Tags(s) MEFSIKQSPLSVQSVVKCIKMKQAPEILGSANGKTPSCEVNRECSVFLSKAQLSSSLQEGVMQKFNGHDA LPFIPADKLKDLTSRVFNGEPGAHDAKLRFESQEMKGIGTPPNTTPIKNGSPEIKLKITKTYMNGKPLFE SSICGDSAADVSQSEENGQKPENKARRNRKRSIKYDSLLEQGLVEAALVSKISSPSDKKIPAKKESCPNT GRDKDHLLKYNVGDLVWSKVSGYPWWPCMVSADPLLHSYTKLKGQKKSARQYHVQFFGDAPERAWIFEKS LVAFEGEGQFEKLCQESAKQAPTKAEKIKLLKPISGKLRAQWEMGIVQAEEAASMSVEERKAKFTFLYVG DQLHLNPQVAKEAGIAAESLGEMAESSGVSEEAAENPKSVREECIPMKRRRRAKLCSSAETLESHPDIGK STPQKTAEADPRRGVGSPPGRKKTTVSMPRSRKGDAASQFLVFCQKHRDEVVAEHPDASGEEIEELLRSQ WSLLSEKQRARYNTKFALVAPVQAEEDSGNVNGKKRNHTKRIQDPTEDAEAEDTPRKRLRTDKHSLRKRD TITDKTARTSSYKAMEAASSLKSQAATKNLSDACKPLKKRNRASTAASSALGFSKSSSPSASLTENEVSD SPGDEPSESPYESADETQTEVSVSSKKSERGVTAKKEYVCQLCEKPGSLLLCEGPCCGAFHLACLGLSRR PEGRFTCSECASGIHSCFVCKESKTDVKRCVVTQCGKFYHEACVKKYPLTVFESRGFRCPLHSCVSCHAS NPSNPRPSKGKMMRCVRCPVAYHSGDACLAAGCSVIASNSIICTAHFTARKGKRHHAHVNVSWCFVCSKG GSLLCCESCPAAFHPDCLNIEMPDGSWFCNDCRAGKKLHFQDIIWVKLGNYRWWPAEVCHPKNVPPNIQK MKHEIGEFPVFFFGSKDYYWTHQARVFPYMEGDRGSRYQGVRGIGRVFKNALQEAEARFREIKLQREARE TQESERKPPPYKHIKVNKPYGKVQIYTADISEIPKCNCKPTDENPCGFDSECLNRMLMFECHPQVCPAGE FCQNQCFTKRQYPETKIIKTDGKGWGLVAKRDIRKGEFVNEYVGELIDEEECMARIKHAHENDITHFYML TIDKDRIIDAGPKGNYSRFMNHSCQPNCETLKWTVNGDTRVGLFAVCDIPAGTELTFNYNLDCLGNEKTV CRCGASNCSGFLGDRPKTSTTLSSEEKGKKTKKKTRRRRAKGEGKRQSEDECFRCGDGGQLVLCDRKFCT KAYHLSCLGLGKRPFGKWECPWHHCDVCGKPSTSFCHLCPNSFCKEHQDGTAFSCTPDGRSYCCEHDLGA ASVRSTKTEKPPPEPGKPKGKRRRRRGWRRVTEGK TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_133331 |
| ORF Size | 4095 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_133331.2, NP_579878.1 |
| RefSeq Size | 7706 bp |
| RefSeq ORF | 4098 bp |
| Locus ID | 7468 |
| UniProt ID | O96028 |
| Protein Families | Druggable Genome, Transcription Factors |
| Protein Pathways | Lysine degradation |
| MW | 152.1 kDa |
| Gene Summary | This gene encodes a protein that contains four domains present in other developmental proteins: a PWWP domain, an HMG box, a SET domain, and a PHD-type zinc finger. It is expressed ubiquitously in early development. Wolf-Hirschhorn syndrome (WHS) is a malformation syndrome associated with a hemizygous deletion of the distal short arm of chromosome 4. This gene maps to the 165 kb WHS critical region and has also been involved in the chromosomal translocation t(4;14)(p16.3;q32.3) in multiple myelomas. Alternative splicing of this gene results in multiple transcript variants encoding different isoforms. Some transcript variants are nonsense-mediated mRNA (NMD) decay candidates, hence not represented as reference sequences. [provided by RefSeq, Jul 2008] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC221401L3 | Lenti-ORF clone of WHSC1 (Myc-DDK-tagged)-Human Wolf-Hirschhorn syndrome candidate 1 (WHSC1), transcript variant 2 |
CNY 12,730.00 |
|
| RC221401L4 | Lenti-ORF clone of WHSC1 (mGFP-tagged)-Human Wolf-Hirschhorn syndrome candidate 1 (WHSC1), transcript variant 2 |
CNY 12,730.00 |
|
| RG221401 | WHSC1 (tGFP-tagged) - Human Wolf-Hirschhorn syndrome candidate 1 (WHSC1), transcript variant 2 |
CNY 11,880.00 |
|
| SC309089 | WHSC1 (untagged)-Human Wolf-Hirschhorn syndrome candidate 1 (WHSC1), transcript variant 2 |
CNY 14,016.00 |
