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Caspase-7 (CASP7) (NM_033340) Human Tagged Lenti ORF Clone
CAT＃: RC221493L4
- LentiORF®
Lenti-ORF clone of CASP7 (mGFP-tagged)-Human caspase 7, apoptosis-related cysteine peptidase (CASP7), transcript variant beta
"NM_033340" in other vectors (4)
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CNY 5,890.00
Product images
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | mGFP |
| Synonyms | CASP-7; CMH-1; ICE-LAP3; LICE2; MCH3 |
| Vector | pLenti-C-mGFP-P2A-Puro |
| E. coli Selection | Chloramphenicol (34 ug/mL) |
| Mammalian Cell Selection | Puromycin |
| Sequence Data |
The ORF insert of this clone is exactly the same as(RC221493).
|
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_033340 |
| ORF Size | 759 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_033340.2 |
| RefSeq Size | 2433 bp |
| RefSeq ORF | 762 bp |
| Locus ID | 840 |
| UniProt ID | P55210 |
| Domains | Peptidase_C14 |
| Protein Families | Druggable Genome, Protease |
| Protein Pathways | Alzheimer's disease, Apoptosis |
| MW | 27.8 kDa |
| Gene Summary | This gene encodes a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. The precursor of the encoded protein is cleaved by caspase 3 and 10, is activated upon cell death stimuli and induces apoptosis. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, May 2012] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC221493 | CASP7 (Myc-DDK-tagged)-Human caspase 7, apoptosis-related cysteine peptidase (CASP7), transcript variant beta |
CNY 3,600.00 |
|
| RC221493L3 | Lenti-ORF clone of CASP7 (Myc-DDK-tagged)-Human caspase 7, apoptosis-related cysteine peptidase (CASP7), transcript variant beta |
CNY 5,890.00 |
|
| RG221493 | CASP7 (tGFP-tagged) - Human caspase 7, apoptosis-related cysteine peptidase (CASP7), transcript variant beta |
CNY 5,200.00 |
|
| SC109027 | CASP7 (untagged)-Human caspase 7, apoptosis-related cysteine peptidase (CASP7), transcript variant beta |
CNY 2,400.00 |
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