United States
Germany
Japan
United Kingdom
China
DSPP (NM_014208) Human Tagged ORF Clone
CAT＃: RC221537
- TrueORF®
DSPP (Myc-DDK-tagged)-Human dentin sialophosphoprotein (DSPP). Note: ORF is codon optimized
ORF Plasmid: tGFP
"NM_014208" in other vectors (4)
Need custom modification / cloning service?
Get a free quote
CNY 13,584.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | DFNA39; DGI1; DMP3; DPP; DSP |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC221537 ORF sequence, codon optimized.
Due to the complexity of NM_014208, the ORF clone is codon optimized for mammalian Expression. The nucleotide sequence differs from the reference sequence, yet the amino acid sequence remains identical. Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAAAATCATTACTTATTTTTGCATTTGGGCGGTGGCCTGGGCCATCCCAGTGCCGCAGTCTAAACCAC TCGAACGGCACGTAGAGAAAAGTATGAACCTCCACCTGTTGGCACGCTCTAACGTCAGTGTTCAGGACGA GCTGAATGCTTCCGGTACAATTAAAGAGTCCGGGGTCCTCGTCCACGAAGGCGATCGGGGGAGGCAGGAG AACACCCAAGATGGGCACAAGGGAGAGGGTAACGGCTCCAAGTGGGCCGAGGTCGGAGGAAAATCCTTTT CCACTTACTCCACACTGGCTAACGAGGAGGGCAACATCGAAGGGTGGAATGGAGATACCGGCAAGGCAGA AACATATGGACACGATGGTATACATGGCAAGGAGGAGAACATCACTGCCAATGGCATCCAAGGGCAGGTT TCTATCATTGACAATGCCGGGGCCACCAATCGGTCTAATACCAACGGGAATACAGATAAGAATACCCAGA ATGGGGACGTGGGCGACGCCGGACACAACGAAGATGTGGCTGTGGTCCAAGAGGATGGCCCCCAGGTGGC AGGATCAAATAACTCAACTGACAATGAAGACGAGATCATCGAGAATTCATGCAGGAATGAAGGAAACACA AGCGAGATCACCCCCCAAATTAACAGTAAGCGGAATGGAACAAAGGAAGCAGAGGTGACCCCAGGCACAG GCGAGGACGCCGGCTTGGATAACAGTGATGGTTCCCCATCTGGCAATGGCGCCGACGAGGATGAGGATGA AGGGAGCGGTGACGATGAGGACGAAGAAGCAGGAAATGGCAAGGATTCCAGCAACAACTCCAAGGGGCAA GAAGGACAGGACCATGGGAAGGAAGATGACCACGATTCTAGTATAGGTCAAAATTCCGACTCTAAGGAGT ACTATGACCCCGAGGGAAAAGAGGACCCCCACAATGAGGTGGACGGCGACAAGACGAGTAAAAGCGAGGA AAATTCCGCTGGCATTCCTGAAGACAATGGCAGCCAACGAATCGAAGATACTCAGAAACTTAACCACCGG GAGTCCAAGAGAGTGGAGAACCGCATCACGAAGGAATCAGAGACACACGCCGTGGGCAAGTCCCAGGATA AAGGAATTGAAATCAAGGGGCCTTCCAGCGGGAACCGCAACATTACAAAAGAGGTGGGGAAGGGTAACGA AGGCAAGGAAGATAAAGGGCAGCACGGAATGATTCTGGGAAAGGGAAACGTGAAAACTCAGGGAGAGGTG GTGAACATCGAGGGTCCCGGCCAAAAGTCTGAACCCGGAAATAAAGTAGGTCACTCTAATACAGGTAGCG ATAGCAACTCTGATGGCTATGACTCTTATGATTTTGACGATAAGTCAATGCAGGGAGATGACCCGAACAG TAGCGACGAATCTAATGGTAACGATGACGCTAACAGCGAGAGCGATAACAACAGCTCTTCTCGGGGGGAT GCATCCTATAATAGCGACGAGAGTAAGGACAACGGAAATGGATCCGATTCCAAAGGTGCAGAGGACGACG ACAGTGACAGTACGTCAGATACAAACAACTCAGACTCCAATGGAAATGGTAATAATGGTAACGATGACAA CGACAAGTCAGATTCAGGCAAGGGAAAATCCGACAGCAGCGATTCTGATAGCTCCGATAGCTCTAACTCA AGTGATTCCTCTGACAGCTCTGACTCTGATTCTAGTGATAGTAACAGCAGCAGCGATTCCGACTCCTCTG ACTCTGATAGCAGTGACTCCAGCGATAGCGATTCTTCCGATAGCTCCAACTCTTCAGATTCTTCAGATAG CAGTGACAGCAGCGACTCATCCGATAGCTCTGACTCCTCAGATAGCAAATCAGATTCCTCCAAAAGCGAG TCCGACTCCTCCGACTCCGATTCAAAAAGCGATAGCAGCGATTCAAACTCATCTGACTCCAGTGATAATA GTGATTCCAGTGATTCTAGCAATTCTAGCAACTCCTCTGACTCCAGCGACTCTAGCGACAGTAGCGACTC CTCTTCTTCCTCAGACAGCTCCAATTCTTCAGATAGCAGCGACAGCAGCGATTCCTCTAACTCCTCTGAG AGTAGCGACTCCAGCGATTCATCCGACAGCGACAGCAGCGACTCTTCAGACTCAAGCAACAGTAACTCTT CAGACTCTGACTCTTCCAATTCATCAGATAGCTCCGACTCATCAAACTCCTCTGATAGCAGCGACAGCTC AGACAGCAGTAACAGCTCTGACTCTTCCGATTCAAGCGATTCCTCCAACTCCTCAGATAGTTCAGACTCC AGTGACAGCTCCGACAGCAGCGACTCCAGCAATTCCTCCGATTCTAATGATTCCTCTAATTCCTCCGACT CAAGCGATTCATCTAACAGTAGTGATTCCAGCAACTCAAGTGATTCCAGCGACAGCTCCGATAGCTCAGA CTCCGACTCTTCTAATAGTTCCGACAGTAGTAATTCTAGCGATAGCTCAGACTCAAGTAATAGTAGTGAC AGCAGTGACTCTAGCGACAGCTCCGATGGTTCTGATAGCGACTCCAGCAACAGGTCAGATTCATCTAATT CATCCGACAGTAGCGACTCATCTGACTCATCTAATAGCTCCGATTCCTCCGACTCTAGCGACTCCAACGA GTCATCTAATAGTTCCGATAGCAGCGACTCAAGCAATTCTAGCGACTCTGACTCATCCGACAGTAGCAAT TCCAGTGACTCCTCTGACAGTTCAAATTCCTCTGATTCCAGTGAATCTAGCAATTCCAGTGACAACTCCA ATTCATCTGATTCCTCCAACTCTAGCGATTCTTCCGATTCTTCCGACTCCAGTAATTCATCCGATTCCAG CAATAGTAGCGACTCTTCCAACTCCTCCGACTCATCAGATAGTAACAGTTCCGATTCAAGCGATAGCAGC AATTCCAGCGACTCTTCAGATAGCTCCGACAGCTCCGATTCCTCTGACAGTAGCGATAGTAGCAATAGTA GTGACTCCTCCGATTCTTCAGATTCTTCCGACAGCTCTAATAGCTCCGACTCCAGTAACAGCTCCGACAG CAGCAACAGCAGTGACTCATCCGATAGCTCCGACTCCTCAGACAGCAGCGACTCTAGCGACTCCTCAGAC TCCTCTGACTCCAGTAACTCCTCAGATTCCAGTGACAGCAGCGATTCCTCCGACAGTTCCGACTCCTCCG ACTCTAGCGATAGTAGTGACAGTTCCGAGTCCTCTGATAGCTCCGACAGTAGTAACAGCAGTGACTCTTC CGATTCATCAGACTCATCTGATTCAAGCGATTCATCAGACTCCTCCGACTCTAGCGATTCAAGTGACTCT AGCAATAGCTCAGACAGCAGCGATTCAAGTGATTCAAGCGACAGTTCTGACAGCAGCAACTCCAGCGACT CCAGCGACTCATCTGAAAGTAGCGATTCAAGTGACTCATCCGACTCCTCTGATAGCAGTGACAGTTCCGA CTCATCTGATTCCAGCGACAGCAGCGATTCCTCTAATTCAAGCGACAGCTCTGATTCAAGCGACAGCTCC GACAGCTCCGATTCCTCAGATAGTAGCGATAGTAGTGATTCCAGCGACTCCAGCGATAGTAGCGATAGCA GTGATTCATCAGATTCAAGCGATTCCTCTGATTCTTCTGACAGTAGTGATAGTAATGAGAGCAGTGACAG TAGTGATAGTAGTGATAGTAGCGATAGTTCAAATTCCAGCGACTCTAGTGATTCCTCTGATTCTTCTGAC AGTACCTCTGACAGCAACGACGAGAGCGATAGTCAGTCTAAGAGTGGCAATGGGAACAATAATGGCTCCG ATTCAGACTCAGATAGTGAAGGGTCTGATAGCAACCATTCTACATCTGATGAT AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC TGGATTACAAGGATGACGACGATAAGGTTTAA >RC221537 representing NM_014208
Red=Cloning site Green=Tags(s) MKIITYFCIWAVAWAIPVPQSKPLERHVEKSMNLHLLARSNVSVQDELNASGTIKESGVLVHEGDRGRQE NTQDGHKGEGNGSKWAEVGGKSFSTYSTLANEEGNIEGWNGDTGKAETYGHDGIHGKEENITANGIQGQV SIIDNAGATNRSNTNGNTDKNTQNGDVGDAGHNEDVAVVQEDGPQVAGSNNSTDNEDEIIENSCRNEGNT SEITPQINSKRNGTKEAEVTPGTGEDAGLDNSDGSPSGNGADEDEDEGSGDDEDEEAGNGKDSSNNSKGQ EGQDHGKEDDHDSSIGQNSDSKEYYDPEGKEDPHNEVDGDKTSKSEENSAGIPEDNGSQRIEDTQKLNHR ESKRVENRITKESETHAVGKSQDKGIEIKGPSSGNRNITKEVGKGNEGKEDKGQHGMILGKGNVKTQGEV VNIEGPGQKSEPGNKVGHSNTGSDSNSDGYDSYDFDDKSMQGDDPNSSDESNGNDDANSESDNNSSSRGD ASYNSDESKDNGNGSDSKGAEDDDSDSTSDTNNSDSNGNGNNGNDDNDKSDSGKGKSDSSDSDSSDSSNS SDSSDSSDSDSSDSNSSSDSDSSDSDSSDSSDSDSSDSSNSSDSSDSSDSSDSSDSSDSSDSKSDSSKSE SDSSDSDSKSDSSDSNSSDSSDNSDSSDSSNSSNSSDSSDSSDSSDSSSSSDSSNSSDSSDSSDSSNSSE SSDSSDSSDSDSSDSSDSSNSNSSDSDSSNSSDSSDSSNSSDSSDSSDSSNSSDSSDSSDSSNSSDSSDS SDSSDSSDSSNSSDSNDSSNSSDSSDSSNSSDSSNSSDSSDSSDSSDSDSSNSSDSSNSSDSSDSSNSSD SSDSSDSSDGSDSDSSNRSDSSNSSDSSDSSDSSNSSDSSDSSDSNESSNSSDSSDSSNSSDSDSSDSSN SSDSSDSSNSSDSSESSNSSDNSNSSDSSNSSDSSDSSDSSNSSDSSNSSDSSNSSDSSDSNSSDSSDSS NSSDSSDSSDSSDSSDSSDSSNSSDSSDSSDSSDSSNSSDSSNSSDSSNSSDSSDSSDSSDSSDSSDSSD SSDSSNSSDSSDSSDSSDSSDSSDSSDSSDSSESSDSSDSSNSSDSSDSSDSSDSSDSSDSSDSSDSSDS SNSSDSSDSSDSSDSSDSSNSSDSSDSSESSDSSDSSDSSDSSDSSDSSDSSDSSDSSNSSDSSDSSDSS DSSDSSDSSDSSDSSDSSDSSDSSDSSDSSDSSDSSDSSDSNESSDSSDSSDSSDSSNSSDSSDSSDSSD STSDSNDESDSQSKSGNGNNNGSDSDSDSEGSDSNHSTSDD SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-RsrII
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_014208 |
| ORF Size | 3903 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_014208.2, NM_014208.3, NP_055023.2 |
| RefSeq Size | 4331 bp |
| RefSeq ORF | 3906 bp |
| Locus ID | 1834 |
| UniProt ID | Q9NZW4 |
| Protein Families | Druggable Genome |
| MW | 131.2 kDa |
| Gene Summary | This gene encodes a member of the small integrin-binding ligand N-linked glycoprotein (SIBLING) family of proteins. The encoded preproprotein is secreted by odontoblasts and proteolytically processed to generate two principal proteins of the dentin extracellular matrix of the tooth, dentin sialoprotein and dentin phosphoprotein. These two protein products may play distinct but related roles in dentin mineralization. Mutations in this gene are associated with dentinogenesis imperfecta and dentin dysplasia. This gene is present in a gene cluster on chromosome 4. Allelic differences due to repeat polymorphisms have been found for this gene. [provided by RefSeq, Jan 2016] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC221537L3 | Lenti-ORF clone of DSPP (Myc-DDK-tagged)-Human dentin sialophosphoprotein (DSPP). Note: ORF is codon optimized |
CNY 14,920.00 |
|
| RC221537L4 | Lenti-ORF clone of DSPP (mGFP-tagged)-Human dentin sialophosphoprotein (DSPP). Note: ORF is codon optimized |
CNY 14,920.00 |
|
| RG221537 | DSPP (tGFP-tagged) - Human dentin sialophosphoprotein (DSPP). Note: ORF is codon optimized |
CNY 15,184.00 |
|
| SC318591 | DSPP (untagged)-Human dentin sialophosphoprotein (DSPP) |
CNY 25,080.00 |
