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TOP3B (NM_003935) Human Tagged ORF Clone
CAT＃: RC223204
- TrueORF®
TOP3B (Myc-DDK-tagged)-Human topoisomerase (DNA) III beta (TOP3B)
ORF Plasmid: tGFP
"NM_003935" in other vectors (4)
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CNY 6,312.00
CNY 7,130.00
| Cited in 1 publication. |
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | TOP3B1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC223204 representing NM_003935
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAAGACTGTGCTCATGGTTGCTGAAAAGCCGTCCTTGGCACAGTCAATTGCCAAAATCCTCTCTAGAG GGAGCCTGTCCTCACACAAAGGGCTGAACGGGGCCTGCTCAGTCCACGAGTACACTGGGACCTTTGCTGG CCAGCCAGTGCGCTTCAAGATGACGTCTGTCTGTGGTCACGTGATGACCCTGGATTTCCTGGGAAAATAC AACAAATGGGACAAAGTGGACCCCGCAGAACTGTTCAGCCAAGCTCCCACGGAGAAGAAAGAAGCTAACC CCAAGCTGAACATGGTGAAGTTCCTGCAGGTGGAGGGCAGAGGCTGCGACTACATCGTGCTGTGGCTGGA CTGCGACAAGGAGGGGGAGAACATCTGCTTTGAGGTTCTTGATGCTGTTCTGCCCGTCATGAACAAGGCC CATGGTGGCGAGAAGACCGTGTTCCGGGCCAGGTTTAGCTCCATCACGGACACAGACATCTGTAATGCCA TGGCCTGCCTAGGCGAGCCTGACCACAACGAGGCGCTCTCAGTGGATGCTCGCCAGGAGCTGGACCTGCG AATCGGCTGTGCATTCACCAGGTTTCAGACTAAATATTTCCAGGGGAAATACGGTGATTTAGACAGCTCT CTCATCTCCTTTGGGCCGTGTCAGACTCCAACCCTGGGATTCTGTGTGGAGAGACATGATAAAATCCAGT CCTTCAAACCAGAGACCTACTGGGTGCTGCAGGCCAAGGTTAACACTGACAAAGACAGATCTCTCCTTTT GGACTGGGACCGAGTAAGAGTGTTTGACCGGGAGATCGCACAGATGTTTTTAAACATGACAAAGCTGGAG AAGGAAGCCCAGGTGGAGGCCACAAGCAGGAAAGAAAAGGCCAAGCAGAGGCCCCTGGCCCTGAACACTG TGGAGATGCTGCGTGTGGCCAGCTCTTCTCTGGGCATGGGGCCGCAGCACGCCATGCAGACGGCTGAGCG GCTCTACACGCAAGGCTACATCAGCTACCCACGGACAGAGACCACCCACTACCCTGAGAACTTTGACCTG AAGGGCTCTCTGCGGCAGCAGGCCAACCACCCCTACTGGGCCGACACGGTGAAGCGGTTGTTAGCAGAAG GTATCAACCGCCCGCGGAAAGGCCATGACGCCGGCGACCATCCCCCCATCACCCCCATGAAGTCTGCCAC AGAGGCCGAATTAGGGGGTGACGCGTGGCGGCTCTATGAGTACATCACCAGACACTTCATCGCCACGGTC AGCCATGACTGCAAGTACCTGCAGAGCACCATCTCCTTCAGAATTGGGCCCGAGCTCTTCACCTGCTCCG GGAAGACCGTCCTCTCACCAGGCTTCACGGAGGTCATGCCCTGGCAGAGCGTGCCCCTGGAGGAGAGCCT GCCCACTTGCCAGCGGGGTGATGCCTTCCCTGTGGGCGAGGTGAAGATGCTGGAGAAGCAGACGAACCCA CCCGACTACCTGACGGAGGCCGAGCTCATCACGCTCATGGAGAAGCATGGCATCGGCACGGATGCCAGCA TCCCTGTGCATATCAACAACATCTGCCAGCGCAACTATGTCACGGTGGAGAGCGGGCGCCGGCTCAAGCC CACCAACCTCGGCATCGTCCTGGTGCACGGCTACTATAAGATTGATGCAGAGCTGGTGCTCCCCACCATC CGCAGTGCAGTGGAGAAGCAGCTGAACCTGATCGCCCAGGGCAAGGCCGACTACCGCCAGGTCCTGGGCC ACACCCTGGACGTGTTCAAGAGGAAGTTCCACTACTTTGTCGACTCCATTGCTGGCATGGATGAGTTGAT GGAGGTGTCTTTCTCGCCCCTGGCGGCCACAGGCAAGCCCCTCTCACGCTGTGGGAAGTGCCACCGCTTC ATGAAGTACATCCAGGCCAAGCCAAGCCGCCTGCACTGCTCCCACTGCGATGAGACCTACACGCTCCCCC AGAACGGCACCATCAAGCTCTACAAGGAGCTCCGCTGCCCTCTGGATGACTTCGAGCTGGTCCTGTGGTC ATCAGGCTCTCGGGGCAAGAGCTACCCGCTGTGCCCCTACTGCTACAACCACCCACCCTTCCGAGACATG AAGAAAGGCATGGGCTGCAACGAGTGTACGCACCCCTCCTGCCAGCACTCGCTGAGCATGCTGGGCATCG GCCAGTGCGTGGAATGTGAGAGCGGGGTGCTGGTGCTGGACCCCACCTCGGGCCCCAAGTGGAAGGTGGC CTGCAACAAGTGCAACGTGGTAGCGCACTGCTTCGAGAACGCCCACCGCGTGCGGGTGTCCGCCGACACC TGCAGTGTCTGTGAGGCCGCCTTGCTTGATGTGGACTTCAACAAGGCCAAGTCCCCACTCCCGGGCGATG AGACGCAGCACATGGGCTGCGTCTTTTGTGACCCCGTCTTCCAGGAGCTGGTGGAGCTGAAGCATGCGGC CTCCTGCCACCCCATGCACCGCGGTGGACCAGGGAGAAGGCAGGGTCGAGGGCGGGGCCGGGCCAGGAGG CCCCCTGGGAAGCCCAACCCCAGACGGCCCAAGGACAAGATGTCAGCCCTGGCCGCCTACTTTGTA AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC TGGATTACAAGGATGACGACGATAAGGTTTAA >RC223204 representing NM_003935
Red=Cloning site Green=Tags(s) MKTVLMVAEKPSLAQSIAKILSRGSLSSHKGLNGACSVHEYTGTFAGQPVRFKMTSVCGHVMTLDFLGKY NKWDKVDPAELFSQAPTEKKEANPKLNMVKFLQVEGRGCDYIVLWLDCDKEGENICFEVLDAVLPVMNKA HGGEKTVFRARFSSITDTDICNAMACLGEPDHNEALSVDARQELDLRIGCAFTRFQTKYFQGKYGDLDSS LISFGPCQTPTLGFCVERHDKIQSFKPETYWVLQAKVNTDKDRSLLLDWDRVRVFDREIAQMFLNMTKLE KEAQVEATSRKEKAKQRPLALNTVEMLRVASSSLGMGPQHAMQTAERLYTQGYISYPRTETTHYPENFDL KGSLRQQANHPYWADTVKRLLAEGINRPRKGHDAGDHPPITPMKSATEAELGGDAWRLYEYITRHFIATV SHDCKYLQSTISFRIGPELFTCSGKTVLSPGFTEVMPWQSVPLEESLPTCQRGDAFPVGEVKMLEKQTNP PDYLTEAELITLMEKHGIGTDASIPVHINNICQRNYVTVESGRRLKPTNLGIVLVHGYYKIDAELVLPTI RSAVEKQLNLIAQGKADYRQVLGHTLDVFKRKFHYFVDSIAGMDELMEVSFSPLAATGKPLSRCGKCHRF MKYIQAKPSRLHCSHCDETYTLPQNGTIKLYKELRCPLDDFELVLWSSGSRGKSYPLCPYCYNHPPFRDM KKGMGCNECTHPSCQHSLSMLGIGQCVECESGVLVLDPTSGPKWKVACNKCNVVAHCFENAHRVRVSADT CSVCEAALLDVDFNKAKSPLPGDETQHMGCVFCDPVFQELVELKHAASCHPMHRGGPGRRQGRGRGRARR PPGKPNPRRPKDKMSALAAYFV SGPTRTRRLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-RsrII
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_003935 |
| ORF Size | 2586 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_003935.5 |
| RefSeq Size | 3124 bp |
| RefSeq ORF | 2589 bp |
| Locus ID | 8940 |
| UniProt ID | O95985 |
| Domains | Topoisom_bac, TOP1Bc, TOP1Ac, TOPRIM, Toprim |
| Protein Pathways | Homologous recombination |
| MW | 96.5 kDa |
| Gene Summary | This gene encodes a DNA topoisomerase, an enzyme that controls and alters the topologic states of DNA during transcription. This enzyme catalyzes the transient breaking and rejoining of a single strand of DNA which allows the strands to pass through one another, thus relaxing the supercoils and altering the topology of DNA. The enzyme interacts with DNA helicase SGS1 and plays a role in DNA recombination, cellular aging and maintenance of genome stability. Low expression of this gene may be related to higher survival rates in breast cancer patients. This gene has a pseudogene on chromosome 22. Alternate splicing results in multiple transcript variants. Additional alternatively spliced transcript variants of this gene have been described, but their full-length nature is not known. [provided by RefSeq, Aug 2013] |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
DNA and RNA Cleavage Complexes and Repair Pathway for TOP3B RNA- and DNA-Protein Crosslinks
,Saha, S;Sun, Y;Huang, SN;Baechler, SA;Pongor, LS;Agama, K;Jo, U;Zhang, H;Tse-Dinh, YC;Pommier, Y;,
Cell reports
,PubMed ID 33378676
[TOP3B]
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Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC223204L3 | Lenti-ORF clone of TOP3B (Myc-DDK-tagged)-Human topoisomerase (DNA) III beta (TOP3B) |
CNY 9,030.00 |
|
| RC223204L4 | Lenti-ORF clone of TOP3B (mGFP-tagged)-Human topoisomerase (DNA) III beta (TOP3B) |
CNY 9,030.00 |
|
| RG223204 | TOP3B (tGFP-tagged) - Human topoisomerase (DNA) III beta (TOP3B) |
CNY 7,890.00 |
|
| SC127952 | TOP3B (untagged)-Human topoisomerase (DNA) III beta (TOP3B) |
CNY 2,950.00 |
