Caldesmon (CALD1) (NM_033138) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	CDM; H-CAD; HCAD; L-CAD; LCAD; NAG22
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC224908 representing NM_033138 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGATGATTTTGAGCGTCGCAGAGAACTTAGAAGGCAAAAGAGGGAGGAGATGCGACTCGAAGCAGAAA GAATCGCCTACCAGAGGAATGACGATGATGAAGAGGAGGCAGCCCGGGAACGGCGCCGCCGAGCCCGACA GGAACGGCTGCGGCAGAAGCAGGAGGAAGAATCCTTGGGACAGGTGACCGACCAGGTGGAGGTGAATGCC CAGAACAGTGTGCCTGACGAGGAGGCCAAGACAACCACCACAAACACTCAAGTGGAAGGGGATGATGAGG CCGCATTCCTGGAGCGCCTGGCTCGGCGTGAGGAAAGACGCCAAAAACGCCTTCAGGAGGCTCTGGAGCG GCAGAAGGAGTTCGACCCAACAATAACAGATGCAAGTCTGTCGCTCCCAAGCAGAAGAATGCAAAATGAC ACAGCAGAAAATGAAACTACCGAGAAGGAAGAAAAAAGTGAAAGTCGCCAAGAAAGATACGAGATAGAGG AAACAGAAACAGTCACCAAGTCCTACCAGAAGAATGATTGGAGGGATGCTGAAGAAAACAAGAAAGAAGA CAAGGAAAAGGAGGAGGAGGAAGAGGAGAAGCCAAAGCGAGGGAGCATTGGAGAAAATCAGGTAGAGGTG ATGGTGGAAGAGAAAACAACTGAAAGCCAGGAGGAAACAGTGGTAATGTCATTAAAAAATGGGCAGATCA GTTCAGAAGAGCCTAAACAAGAGGAGGAGAGGGAACAAGGTTCAGATGAGATTTCCCATCATGAAAAGAT GGAAGAGGAAGACAAGGAAAGAGCTGAGGCAGAGAGGGCAAGGTTGGAAGCAGAAGAAAGAGAAAGAATT AAAGCCGAGCAAGACAAAAAGATAGCAGATGAACGAGCAAGAATTGAAGCAGAAGAAAAAGCAGCTGCCC AAGAAAGAGAAAGGAGAGAGGCAGAAGAGAGGGAAAGGATGAGGGAGGAAGAGAAAAGGGCAGCAGAGGA GAGGCAGAGGATAAAGGAGGAAGAGAAAAGGGCAGCAGAGGAGAGGCAGAGGATAAAGGAGGAAGAGAAA AGGGCAGCAGAGGAGAGGCAGAGGATAAAAGAGGAAGAGAAAAGGGCAGCAGAGGAGAGGCAAAGGGCCA GGGCAGAGGAGGAAGAGAAGGCTAAGGTAGAAGAGCAGAAACGTAACAAGCAGCTAGAAGAGAAAAAACA TGCCATGCAAGAGACAAAGATAAAAGGGGAAAAGGTAGAACAGAAAATAGAAGGGAAATGGGTAAATGAA AAGAAAGCACAAGAAGATAAACTTCAGACAGCTGTCCTAAAGAAACAGGGAGAAGAGAAGGGAACTAAAG TGCAAGCTAAAAGAGAAAAGCTCCAAGAAGACAAGCCTACCTTCAAAAAAGAAGAGATCAAAGATGAAAA GATTAAAAAGGACAAAGAACCCAAAGAAGAAGTTAAGAGCTTCATGGATCGAAAGAAGGGATTTACAGAA GTTAAGTCGCAGAATGGAGAATTCATGACCCACAAACTTAAACATACTGAGAATACTTTCAGCCGCCCTG GAGGGAGGGCCAGCGTGGACACCAAGGAGGCTGAGGGCGCCCCCCAGGTGGAAGCCGGCAAAAGGCTGGA GGAGCTTCGTCGTCGTCGCGGGGAGACCGAGAGCGAAGAGTTCGAGAAGCTCAAACAGAAGCAGCAGGAG GCGGCTTTGGAGCTGGAGGAACTCAAGAAAAAGAGGGAGGAGAGAAGGAAGGTCCTGGAGGAGGAAGAGC AGAGGAGGAAGCAGGAGGAAGCCGATCGAAAACTCAGAGAGGAGGAAGAGAAGAGGAGGCTAAAGGAAGA GATTGAAAGGCGAAGAGCAGAAGCTGCTGAGAAACGCCAGAAGATGCCAGAAGATGGCTTGTCAGATGAC AAGAAACCATTCAAGTGTTTCACTCCTAAAGGTTCATCTCTCAAGATAGAAGAGCGAGCAGAATTTTTGA ATAAGTCTGTGCAGAAAAGCAGTGGTGTCAAATCGACCCATCAAGCAGCAATAGTCTCCAAGATTGACAG CAGACTGGAGCAGTATACCAGTGCAATTGAGGGAACAAAAAGCGCAAAACCTACAAAGCCGGCAGCCTCG GATCTTCCTGTTCCTGCTGAAGGTGTACGCAACATCAAGAGTATGTGGGAGAAAGGGAATGTGTTTTCAT CCCCCACTGCAGCAGGCACACCAAATAAGGAAACTGCTGGCTTGAAGGTAGGGGTTTCTAGCCGCATCAA TGAATGGCTAACTAAAACCCCAGATGGAAACAAGTCACCTGCTCCCAAACCTTCTGACTTGAGACCAGGA GACGTATCCAGCAAGCGGAACCTCTGGGAAAAGCAATCTGTGGATAAGGTCACTTCCCCCACTAAGGTT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC224908 representing NM_033138 Red=Cloning site Green=Tags(s) MDDFERRRELRRQKREEMRLEAERIAYQRNDDDEEEAARERRRRARQERLRQKQEEESLGQVTDQVEVNA QNSVPDEEAKTTTTNTQVEGDDEAAFLERLARREERRQKRLQEALERQKEFDPTITDASLSLPSRRMQND TAENETTEKEEKSESRQERYEIEETETVTKSYQKNDWRDAEENKKEDKEKEEEEEEKPKRGSIGENQVEV MVEEKTTESQEETVVMSLKNGQISSEEPKQEEEREQGSDEISHHEKMEEEDKERAEAERARLEAEERERI KAEQDKKIADERARIEAEEKAAAQERERREAEERERMREEEKRAAEERQRIKEEEKRAAEERQRIKEEEK RAAEERQRIKEEEKRAAEERQRARAEEEEKAKVEEQKRNKQLEEKKHAMQETKIKGEKVEQKIEGKWVNE KKAQEDKLQTAVLKKQGEEKGTKVQAKREKLQEDKPTFKKEEIKDEKIKKDKEPKEEVKSFMDRKKGFTE VKSQNGEFMTHKLKHTENTFSRPGGRASVDTKEAEGAPQVEAGKRLEELRRRRGETESEEFEKLKQKQQE AALELEELKKKREERRKVLEEEEQRRKQEEADRKLREEEEKRRLKEEIERRRAEAAEKRQKMPEDGLSDD KKPFKCFTPKGSSLKIEERAEFLNKSVQKSSGVKSTHQAAIVSKIDSRLEQYTSAIEGTKSAKPTKPAAS DLPVPAEGVRNIKSMWEKGNVFSSPTAAGTPNKETAGLKVGVSSRINEWLTKTPDGNKSPAPKPSDLRPG DVSSKRNLWEKQSVDKVTSPTKV TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_033138
ORF Size	2379 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_033138.4
RefSeq Size	5241 bp
RefSeq ORF	2382 bp
Locus ID	800
UniProt ID	Q05682
Domains	Caldesmon
Protein Pathways	Vascular smooth muscle contraction
MW	93.2 kDa
Gene Summary	This gene encodes a calmodulin- and actin-binding protein that plays an essential role in the regulation of smooth muscle and nonmuscle contraction. The conserved domain of this protein possesses the binding activities to Ca(2+)-calmodulin, actin, tropomyosin, myosin, and phospholipids. This protein is a potent inhibitor of the actin-tropomyosin activated myosin MgATPase, and serves as a mediating factor for Ca(2+)-dependent inhibition of smooth muscle contraction. Alternative splicing of this gene results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Jul 2008]

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