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CMG1 (IFT74) (NM_001099224) Human Tagged ORF Clone
CAT＃: RC224927
- TrueORF®
IFT74 (Myc-DDK-tagged)-Human intraflagellar transport 74 homolog (Chlamydomonas) (IFT74), transcript variant 4
ORF Plasmid: tGFP
"NM_001099224" in other vectors (4)
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CNY 3,990.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | CCDC2; CMG-1; CMG1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC224927 representing NM_001099224
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCAGCAATCACAAATCTTCAGCAGCTCGCCCTGTTTCAAGAGGTGGAGTTGGGTTAACAGGAAGGC CTCCTTCTGGGATACGACCCCTATCAGGAAATATTCGAGTGGCAACTGCAATGCCACCTGGGACAGCAAG ACCAGGTTCTCGTGGTTGTCCCATAGGGACTGGTGGAGTTCTGTCTTCTCAAATCAAAGTTGCCCATCGC CCTGTAACACAACAAGGTTTGACTGGAATGAAAACTGGGACGAAAGGTCCCCAGAGGCAAATTTTAGACA AATCTTACTATCTTGGGCTTCTTAGAAGTAAAATAAGTGAACTTACAACTGAAGTTAATAAACTTCAGAA GGGAATAGAAATGTACAATCAAGAGAATTCAGTATATTTGTCATATGAAAAGAGGGCTGAGACTTTAGCT GTTGAGATAAAAGAGCTTCAAGGACAACTAGCAGACTACAACATGTTGGTAGATAAACTTAATACCAACA CTGAAATGGAAGAAGTAATGAATGATTACAATATGCTTAAAGCTCAAAATGATCGAGAAACACAAAGTTT GGATGTCATATTTACTGAAAGACAAGCGAAAGAAAAACAAATCAGAAGTGTCGAAGAAGAAATTGAACAG GAAAAACAAGCAACAGATGACATTATCAAAAATATGTCTTTTGAAAACCAAGTCAAGTACCTAGAGATGA AAACCACAAATGAGAAACTGTTACAGGAATTAGATACACTTCAACAACAATTGGATTCACAGAACATGAA AAAAGAGAGCCTGGAAGCAGAAATAGCTCACTCCCAGGTGAAACAGGAGGCGGTATTGCTGCATGAAAAA CTTTATGAGTTGGAGTCCCATCGAGATCAAATGATTGCAGAAGACAAAAGCATAGGATCTCCAATGGAAG AGAGAGAGAAATTACTTAAGCAGATTAAAGATGATAATCAGGAAATAGCCAGCATGGAAAGACAGTTAAC AGATACAAAAGAAAAGATAAATCAGTTTATTGAAGAAATTAGACAACTTGACATGGATTTAGAGGAACAC CAAGATCCTACCAACTATGGATGGAAAATACTTGAAAAAAATACAGGAAGTTTCAAAAAGCAAGTT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC224927 representing NM_001099224
Red=Cloning site Green=Tags(s) MASNHKSSAARPVSRGGVGLTGRPPSGIRPLSGNIRVATAMPPGTARPGSRGCPIGTGGVLSSQIKVAHR PVTQQGLTGMKTGTKGPQRQILDKSYYLGLLRSKISELTTEVNKLQKGIEMYNQENSVYLSYEKRAETLA VEIKELQGQLADYNMLVDKLNTNTEMEEVMNDYNMLKAQNDRETQSLDVIFTERQAKEKQIRSVEEEIEQ EKQATDDIIKNMSFENQVKYLEMKTTNEKLLQELDTLQQQLDSQNMKKESLEAEIAHSQVKQEAVLLHEK LYELESHRDQMIAEDKSIGSPMEEREKLLKQIKDDNQEIASMERQLTDTKEKINQFIEEIRQLDMDLEEH QDPTNYGWKILEKNTGSFKKQV TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001099224 |
| ORF Size | 1116 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001099224.2 |
| RefSeq Size | 1571 bp |
| RefSeq ORF | 1119 bp |
| Locus ID | 80173 |
| UniProt ID | Q96LB3 |
| MW | 42.2 kDa |
| Gene Summary | This gene encodes a core intraflagellar transport (IFT) protein which belongs to a multi-protein complex involved in the transport of ciliary proteins along axonemal microtubules. IFT proteins are found at the base of the cilium as well as inside the cilium, where they assemble into long arrays between the ciliary base and tip. This protein, together with intraflagellar transport protein 81, binds and transports tubulin within cilia and is required for ciliogenesis. Naturally occurring mutations in this gene are associated with amyotrophic lateral sclerosis--frontotemporal dementia and Bardet-Biedl Syndrome. [provided by RefSeq, Mar 2017] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC224927L3 | Lenti-ORF clone of IFT74 (Myc-DDK-tagged)-Human intraflagellar transport 74 homolog (Chlamydomonas) (IFT74), transcript variant 4 |
CNY 5,890.00 |
|
| RC224927L4 | Lenti-ORF clone of IFT74 (mGFP-tagged)-Human intraflagellar transport 74 homolog (Chlamydomonas) (IFT74), transcript variant 4 |
CNY 5,890.00 |
|
| RG224927 | IFT74 (tGFP-tagged) - Human intraflagellar transport 74 homolog (Chlamydomonas) (IFT74), transcript variant 4 |
CNY 4,370.00 |
|
| SC316512 | IFT74 (untagged)-Human intraflagellar transport 74 homolog (Chlamydomonas) (IFT74), transcript variant 4 |
CNY 7,220.00 |
