PLSCR4 (NM_001128305) Human Tagged ORF Clone

CAT#: RC225476

PLSCR4 (Myc-DDK-tagged)-Human phospholipid scramblase 4 (PLSCR4), transcript variant 3

ORF Plasmid: DDK tGFP



  "NM_001128305" in other vectors (4)

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CNY 2,400.00

CNY 3,990.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (5)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

CNY 300.00


Rabbit Polyclonal Anti-PLSCR4 Antibody
    • 50 ug

CNY 5,200.00

Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Synonyms TRA1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC225476 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTCAGGTGTGGTACCCACAGCCCCTGAACAGCCTGCAGGTGAAATGGAAAATCAAACAAAACCACCAG
ATCCAAGGCCTGATGCTCCTCCTGAATACAATTCTCATTTTTTACCAGGACCCCCTGGAACAGCTGTCCC
TCCACCTACTGGCTACCCAGGAGGCTTGCCTATGGGATACTACAGTCCACAGCAACCCAGTACCTTCCCT
TTGTACCAGCCAGTTGGTGGTATCCATCCTGTCCGGTATCAGCCTGGCAAATATCCTATGCCAAATCAGT
CTGTTCCAATAACATGGATGCCAGGGCCAACTCCTATGGCAAACTGCCCTCCTGGTCTGGAATACTTAGT
TCAGTTGGACAACATACATGTTCTTCAGCATTTTGAGCCTCTGGAAATGATGACATGTTTTGAAACTAAT
AATAGATATGATATTAAAAACAACTCAGACCAGATGGTTTACATTGTAACCGAAGACACAGATGACTTTA
CCAGGAATGCCTATCGGACACTAAGGCCCTTCGTCCTCCGGGTCACTGATTGTATGGGCCGAGAAATCAT
GACAATGCAGAGACCCTTCAGATGCACCTGCTGTTGCTTCTGTTGCCCCTCTGCCAGACAAGAGCTGGAG
GTGCAGTGTCCTCCTGGTGTCACCATTGGCTTTGTTGCGGAACATTGGAACCTGTGCAGGGCGGTGTACA
GCATCCAAAATGAGAAGAAAGAAAATGTGATGAGAGTTCGTGGGCCATGCTCAACCTATGGCTGTGGTTC
AGATTCTGTTTTTGAGGTCAAATCCCTTGATGGCATATCCAACATCGGCAGTATTATCCGGAAGTGGAAT
GGTTTGTTATCAGCAATGGCAGATGCTGACCATTTTGACATTCACTTCCCACTAGACCTGGATGTGAAGA
TGAAAGCCATGATTTTTGGAGCTTGCTTCCTCATTGACTTCATGTATTTTGAAAGATCTCCACCACAACG
TTCAAGA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC225476 protein sequence
Red=Cloning site Green=Tags(s)

MSGVVPTAPEQPAGEMENQTKPPDPRPDAPPEYNSHFLPGPPGTAVPPPTGYPGGLPMGYYSPQQPSTFP
LYQPVGGIHPVRYQPGKYPMPNQSVPITWMPGPTPMANCPPGLEYLVQLDNIHVLQHFEPLEMMTCFETN
NRYDIKNNSDQMVYIVTEDTDDFTRNAYRTLRPFVLRVTDCMGREIMTMQRPFRCTCCCFCCPSARQELE
VQCPPGVTIGFVAEHWNLCRAVYSIQNEKKENVMRVRGPCSTYGCGSDSVFEVKSLDGISNIGSIIRKWN
GLLSAMADADHFDIHFPLDLDVKMKAMIFGACFLIDFMYFERSPPQRSR

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_001128305
ORF Size 987 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_001128305.2
RefSeq Size 3400 bp
RefSeq ORF 990 bp
Locus ID 57088
UniProt ID Q9NRQ2
MW 37 kDa
Gene Summary May mediate accelerated ATP-independent bidirectional transbilayer migration of phospholipids upon binding calcium ions that results in a loss of phospholipid asymmetry in the plasma membrane. May play a central role in the initiation of fibrin clot formation, in the activation of mast cells and in the recognition of apoptotic and injured cells by the reticuloendothelial system.[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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