SLC39A14 (NM_001135153) Human Tagged ORF Clone

CAT#: RC225847

  • TrueORF®

SLC39A14 (Myc-DDK-tagged)-Human solute carrier family 39 (zinc transporter), member 14 (SLC39A14), transcript variant 3

ORF Plasmid: DDK tGFP



  "NM_001135153" in other vectors (4)

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CNY 3,656.00

CNY 3,990.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (5)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

CNY 300.00


Rabbit Polyclonal ZIP14 Antibody
    • 100 ug

CNY 5,808.00

Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Synonyms cig19; HCIN; HMNDYT2; LZT-Hs4; NET34; ZIP14
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC225847 representing NM_001135153
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAAGCTGCTGCTGCTGCACCCGGCCTTCCAGAGCTGCCTCCTGCTGACCCTGCTTGGCTTATGGAGAA
CCACCCCTGAGGCTCACGCTTCATCCCTGGGTGCACCAGCTATCAGCGCTGCCTCCTTCCTGCAGGATCT
AATACATCGGTATGGCGAGGGTGACAGCCTCACTCTGCAGCAGCTGAAGGCCCTACTCAACCACCTGGAT
GTGGGAGTGGGCCGGGGTAATGTCACCCAGCACGTGCAAGGACACAGGAACCTCTCCACGTGCTTTAGTT
CTGGAGACCTCTTCACTGCCCACAATTTCAGCGAGCAGTCGCGGATTGGGAGCAGCGAGCTCCAGGAGTT
CTGCCCCACCATCCTCCAGCAGCTGGATTCCCGGGCCTGCACCTCGGAGAACCAGGAAAACGAGGAGAAT
GAGCAGACGGAGGAGGGGCGGCCAAGCGCTGTTGAAGTGTGGGGATACGGTCTCCTCTGTGTGACCGTCA
TCTCCCTCTGCTCCCTCCTGGGGGCCAGCGTGGTGCCCTTCATGAAGAAGACCTTTTACAAGAGGCTGCT
GCTCTACTTCATAGCTCTGGCGATTGGAACCCTCTACTCCAACGCCCTCTTCCAGCTCATCCCGGAGGCA
TTTGGTTTCAACCCTCTGGAAGATTATTATGTCTCCAAGTCTGCAGTGGTGTTTGGGGGCTTTTATCTTT
TCTTTTTCACAGAGAAGATCTTGAAGATTCTTCTTAAGCAGAAAAATGAGCATCATCATGGACACAGCCA
TTATGCCTCTGAGTCGCTTCCCTCCAAGAAGGACCAGGAGGAGGGGGTGATGGAGAAGCTGCAGAACGGG
GACCTGGACCACATGATTCCTCAGCACTGCAGCAGTGAGCTGGACGGCAAGGCGCCCATGGTGGACGAGA
AGGTCATTGTGGGCTCGCTCTCTGTGCAGGACCTGCAGGCTTCCCAGAGTGCTTGCTACTGGCTGAAAGG
TGTCCGCTACTCTGATATCGGCACTCTGGCCTGGATGATCACTCTGAGCGACGGCCTCCATAATTTCATC
GATGGCCTGGCCATCGGTGCTTCCTTCACTGTGTCAGTTTTCCAAGGCATCAGCACCTCGGTGGCCATCC
TCTGTGAGGAGTTCCCACATGAGCTAGGAGACTTTGTCATCCTGCTCAACGCTGGGATGAGCATCCAACA
AGCTCTCTTCTTCAACTTCCTTTCTGCCTGCTGCTGCTACCTGGGTCTGGCCTTTGGCATCCTGGCCGGC
AGCCACTTCTCTGCCAACTGGATTTTTGCGCTAGCTGGAGGAATGTTCTTGTATATTTCTCTGGCTGATA
TGTTCCCTGAGATGAATGAGGTCTGTCAAGAGGATGAAAGGAAGGGCAGCATCTTGATTCCATTTATCAT
CCAGAACCTGGGCCTCCTGACTGGATTCACCATCATGGTGGTCCTCACCATGTATTCAGGACAGATCCAG
ATTGGG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC225847 representing NM_001135153
Red=Cloning site Green=Tags(s)

MKLLLLHPAFQSCLLLTLLGLWRTTPEAHASSLGAPAISAASFLQDLIHRYGEGDSLTLQQLKALLNHLD
VGVGRGNVTQHVQGHRNLSTCFSSGDLFTAHNFSEQSRIGSSELQEFCPTILQQLDSRACTSENQENEEN
EQTEEGRPSAVEVWGYGLLCVTVISLCSLLGASVVPFMKKTFYKRLLLYFIALAIGTLYSNALFQLIPEA
FGFNPLEDYYVSKSAVVFGGFYLFFFTEKILKILLKQKNEHHHGHSHYASESLPSKKDQEEGVMEKLQNG
DLDHMIPQHCSSELDGKAPMVDEKVIVGSLSVQDLQASQSACYWLKGVRYSDIGTLAWMITLSDGLHNFI
DGLAIGASFTVSVFQGISTSVAILCEEFPHELGDFVILLNAGMSIQQALFFNFLSACCCYLGLAFGILAG
SHFSANWIFALAGGMFLYISLADMFPEMNEVCQEDERKGSILIPFIIQNLGLLTGFTIMVVLTMYSGQIQ
IG

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_001135153
ORF Size 1476 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_001135153.3
RefSeq Size 4740 bp
RefSeq ORF 1479 bp
Locus ID 23516
UniProt ID Q15043
Protein Families Transmembrane
MW 54.2 kDa
Gene Summary This gene encodes a member of the the SLC39A family of divalent metal transporters that mediates the cellular uptake of manganese, zinc, iron, and cadmium. The encoded protein contains eight transmembrane domains, a histidine-rich motif, and a metalloprotease motif, and is expressed on the plasma membrane and the endocytic vesicle membrane. It is an important transporter of nontransferrin-bound iron and a critical regulator of manganese homeostasis. Naturally occurring mutations in this gene are associated with neurodegeneration with brain iron accumulation and early-onset parkinsonism-dystonia with hypermanganesemia. [provided by RefSeq, May 2017]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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