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PEX5 (NM_001131023) Human Tagged ORF Clone
CAT＃: RC226067
PEX5 (Myc-DDK-tagged)-Human peroxisomal biogenesis factor 5 (PEX5), transcript variant 1
ORF Plasmid: tGFP
"NM_001131023" in other vectors (4)
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CNY 7,304.00
Product images
CNY 300.00
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | PBD2A; PBD2B; PTS1-BP; PTS1R; PXR1; RCDP5 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC226067 representing NM_001131023
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCAATGCGGGAGCTGGTGGAGGCCGAATGCGGGGGTGCCAACCCGCTCATGAAGCTCGCCGGGCACT TCACCCAGGACAAGGCCCTTCGGCAGGAGGGATTGAGGCCTGGCCCCTGGCCCCCCGGAGCCCCGGCCTC TGAGGCAGTGAGTGTTCTTGAGGTGGAAAGCCCAGGTGCAGCCTCTGAGGCAGCCTCCAAGCCTTTGGGA GTAGCTTCTGAAGATGAGTTGGTGGCTGAATTCCTGCAGGACCAGAATGCACCCCTTGTGTCCCGTGCCC CTCAGACCTTCAAGATGGATGACCTCCTGGCTGAGATGCAGCAGATTGAGCAGTCAAACTTCCGCCAGGC TCCCCAGAGAGCCCCTGGTGTGGCAGACTTGGCCTTGTCTGAGAACTGGGCCCAGGAGTTTCTTGCAGCT GGAGATGCTGTGGATGTAACTCAGGATTATAATGAGACTGACTGGTCCCAAGAATTCATCTCTGAAGTTA CAGACCCCTTGTCTGTGTCCCCTGCCCGCTGGGCTGAGGAATATTTGGAGCAATCAGAGGAGAAGCTGTG GCTGGGAGAACCTGAGGGAACAGCCACCGATCGCTGGTATGATGAATATCATCCTGAGGAGGATCTGCAG CACACGGCCAGTGACTTTGTGGCCAAAGTGGATGACCCCAAATTGGCTAATTCTGAGTTCCTGAAATTCG TGCGGCAGATTGGCGAAGGGCAGGTGTCCCTGGAGTCCGGTGCAGGGTCGGGCCGAGCTCAGGCAGAACA GTGGGCAGCAGAGTTTATACAGCAGCAGGGTACATCAGATGCCTGGGTTGACCAGTTCACAAGACCAGTA AACACATCTGCCCTTGATATGGAGTTTGAACGAGCCAAGTCAGCTATAGAGTCTGATGTCGATTTCTGGG ACAAGTTGCAGGCAGAGTTGGAGGAGATGGCAAAACGGGATGCTGAGGCCCACCCCTGGCTTTCTGACTA TGATGACCTTACGTCAGCTACCTATGATAAGGGGTACCAGTTTGAGGAGGAGAACCCCTTGCGTGATCAC CCTCAGCCTTTTGAAGAAGGGCTGCGGCGCCTTCAGGAGGGGGACCTGCCAAATGCTGTGCTGCTTTTTG AGGCAGCTGTGCAGCAGGATCCTAAGCACATGGAAGCTTGGCAGTATCTGGGTACCACCCAGGCAGAGAA TGAACAAGAACTATTAGCCATCAGTGCATTGCGGAGGTGTCTGGAGCTAAAGCCAGATAACCAGACAGCA CTGATGGCGCTGGCTGTGAGCTTCACCAACGAGTCCCTGCAGCGACAGGCCTGTGAAACCCTACGAGACT GGCTGCGGTACACACCAGCCTATGCCCATCTGGTGACACCTGCTGAAGAAGGGGCTGGTGGGGCAGGACT GGGCCCCAGCAAGCGTATCCTGGGATCTCTCTTGTCTGACTCCCTGTTTCTTGAAGTGAAAGAGCTCTTC CTGGCAGCTGTGCGGCTGGACCCTACCTCCATTGACCCTGATGTGCAGTGTGGCTTGGGAGTCCTTTTCA ACCTGAGTGGGGAGTATGACAAGGCCGTGGACTGCTTCACAGCTGCCCTCAGCGTTCGTCCCAATGACTA TTTGCTGTGGAATAAGCTAGGCGCCACCCTGGCCAATGGAAACCAGAGTGAAGAAGCAGTAGCTGCGTAC CGCCGGGCCCTCGAGCTCCAGCCTGGCTATATCCGGTCCCGCTATAACCTGGGCATCAGCTGCATCAACC TCGGGGCTCACCGGGAGGCTGTGGAGCACTTTCTGGAGGCCCTGAACATGCAGAGGAAAAGCCGGGGCCC CCGGGGTGAAGGAGGTGCCATGTCGGAGAACATCTGGAGCACCCTGCGTTTGGCATTGTCTATGTTAGGC CAGAGCGATGCCTATGGGGCAGCCGACGCGCGGGATCTGTCCACCCTCCTAACTATGTTTGGCCTGCCCC AG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC226067 representing NM_001131023
Red=Cloning site Green=Tags(s) MAMRELVEAECGGANPLMKLAGHFTQDKALRQEGLRPGPWPPGAPASEAVSVLEVESPGAASEAASKPLG VASEDELVAEFLQDQNAPLVSRAPQTFKMDDLLAEMQQIEQSNFRQAPQRAPGVADLALSENWAQEFLAA GDAVDVTQDYNETDWSQEFISEVTDPLSVSPARWAEEYLEQSEEKLWLGEPEGTATDRWYDEYHPEEDLQ HTASDFVAKVDDPKLANSEFLKFVRQIGEGQVSLESGAGSGRAQAEQWAAEFIQQQGTSDAWVDQFTRPV NTSALDMEFERAKSAIESDVDFWDKLQAELEEMAKRDAEAHPWLSDYDDLTSATYDKGYQFEEENPLRDH PQPFEEGLRRLQEGDLPNAVLLFEAAVQQDPKHMEAWQYLGTTQAENEQELLAISALRRCLELKPDNQTA LMALAVSFTNESLQRQACETLRDWLRYTPAYAHLVTPAEEGAGGAGLGPSKRILGSLLSDSLFLEVKELF LAAVRLDPTSIDPDVQCGLGVLFNLSGEYDKAVDCFTAALSVRPNDYLLWNKLGATLANGNQSEEAVAAY RRALELQPGYIRSRYNLGISCINLGAHREAVEHFLEALNMQRKSRGPRGEGGAMSENIWSTLRLALSMLG QSDAYGAADARDLSTLLTMFGLPQ TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001131023 |
| ORF Size | 1962 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001131023.2 |
| RefSeq ORF | 1965 bp |
| Locus ID | 5830 |
| UniProt ID | P50542 |
| Protein Families | Druggable Genome |
| MW | 72.1 kDa |
| Gene Summary | The product of this gene binds to the C-terminal PTS1-type tripeptide peroxisomal targeting signal (SKL-type) and plays an essential role in peroxisomal protein import. Peroxins (PEXs) are proteins that are essential for the assembly of functional peroxisomes. The peroxisome biogenesis disorders (PBDs) are a group of genetically heterogeneous autosomal recessive, lethal diseases characterized by multiple defects in peroxisome function. The peroxisomal biogenesis disorders are a heterogeneous group with at least 14 complementation groups and with more than 1 phenotype being observed in cases falling into particular complementation groups. Although the clinical features of PBD patients vary, cells from all PBD patients exhibit a defect in the import of one or more classes of peroxisomal matrix proteins into the organelle. Defects in this gene are a cause of neonatal adrenoleukodystrophy (NALD), a cause of Zellweger syndrome (ZWS) as well as may be a cause of infantile Refsum disease (IRD). Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Oct 2008] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC226067L3 | Lenti-ORF clone of PEX5 (Myc-DDK-tagged)-Human peroxisomal biogenesis factor 5 (PEX5), transcript variant 1 |
CNY 6,460.00 |
|
| RC226067L4 | Lenti-ORF clone of PEX5 (mGFP-tagged)-Human peroxisomal biogenesis factor 5 (PEX5), transcript variant 1 |
CNY 6,460.00 |
|
| RG226067 | PEX5 (tGFP-tagged) - Human peroxisomal biogenesis factor 5 (PEX5), transcript variant 1 |
CNY 5,040.00 |
|
| SC326148 | PEX5 (untagged)-Human peroxisomal biogenesis factor 5 (PEX5), transcript variant 1 |
CNY 10,450.00 |
