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AREB6 (ZEB1) (NM_001128128) Human Tagged ORF Clone
CAT＃: RC226329
- TrueORF®
ZEB1 (Myc-DDK-tagged)-Human zinc finger E-box binding homeobox 1 (ZEB1), transcript variant 1
ORF Plasmid: tGFP
"NM_001128128" in other vectors (6)
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CNY 11,568.00
| Cited in 2 publications. |
Product images
CNY 300.00
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | AREB6; BZP; DELTAEF1; FECD6; NIL2A; PPCD3; TCF8; ZFHEP; ZFHX1A |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC226329 representing NM_001128128
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAAAGTTACAAATTATAATACTGTGGTAGAAACAAATTCAGATTCAGATGATGAAGACAAACTGCATA TTGTGGAAGAAGAAAGTGTTACAGATGCAGCTGACTGTGAAGGTGTACCAGAGGATGACCTGCCAACAGA CCAGACAGTGTTACCAGGGAGGAGCAGTGAAAGAGAAGGGAATGCTAAGAACTGCTGGGAGGATGACACA GGAAAGGAAGGGCAAGAAATCCTGGGGCCTGAAGCTCAGGCAGATGAAGCAGGATGTACAGTAAAAGATG ATGAATGCGAGTCAGATGCAGAAAATGAGCAAAACCATGATCCTAATGTTGAAGAGTTTCTACAACAACA AGACACTGCTGTCATTTTTCCTGAGGCACCTGAAGAGGACCAGAGGCAGGGCACACCAGAAGCCAGTGGT CATGATGAAAATGGAACACCAGATGCATTTTCACAATTACTCACCTGTCCATATTGTGATAGAGGCTATA AACGCTTTACCTCTCTGAAAGAACACATTAAATATCGTCATGAAAAGAATGAAGATAACTTTAGTTGCTC CCTGTGCAGTTACACCTTTGCATACAGAACCCAACTTGAACGTCACATGACATCACATAAATCAGGAAGA GATCAAAGACATGTGACGCAGTCTGGGTGTAATCGTAAATTCAAATGCACTGAGTGTGGAAAAGCTTTCA AATACAAACATCACCTAAAAGAGCACTTAAGAATTCACAGTGGAGAGAAGCCATATGAATGCCCAAACTG CAAGAAACGCTTTTCCCATTCTGGCTCCTATAGCTCACACATAAGCAGTAAGAAATGTATCAGCTTGATA CCTGTGAATGGGCGACCAAGAACAGGACTCAAGACATCTCAGTGTTCTTCACCGTCTCTTTCAGCATCAC CAGGCAGTCCCACACGACCACAGATACGGCAAAAGATAGAGAATAAACCCCTTCAAGAACAACTTTCTGT TAACCAAATTAAAACTGAACCTGTGGATTATGAATTCAAACCCATAGTGGTTGCTTCAGGAATCAACTGT TCAACCCCTTTACAAAATGGGGTTTTCACTGGTGGTGGCCCATTACAGGCAACCAGTTCTCCTCAGGGCA TGGTGCAAGCTGTTGTTCTGCCAACAGTTGGTTTGGTGTCTCCCATAAGTATCAATTTAAGTGATATTCA GAATGTACTTAAAGTGGCGGTAGATGGTAATGTAATAAGGCAAGTGTTGGAGAATAATCAAGCCAATCTT GCATCCAAAGAACAAGAAACAATCAATGCTTCACCCATACAACAAGGTGGCCATTCTGTTATTTCAGCCA TCAGTCTTCCTTTGGTTGATCAAGATGGAACAACCAAAATTATCATCAACTACAGTCTTGAGCAGCCTAG CCAACTTCAAGTTGTTCCTCAAAATTTAAAAAAAGAAAATCCAGTCGCTACAAACAGTTGTAAAAGTGAA AAGTTACCAGAAGATCTTACTGTTAAGTCTGAGAAGGACAAAAGCTTTGAAGGGGGGGTGAATGATAGCA CTTGTCTTCTGTGTGATGATTGTCCAGGAGATATTAATGCACTTCCAGAATTAAAGCACTATGACCTAAA GCAGCCTACTCAGCCTCCTCCACTCCCTGCAGCAGAAGCTGAGAAGCCTGAGTCCTCTGTTTCATCAGCT ACTGGAGATGGCAATTTGTCTCCTAGTCAGCCACCTTTAAAGAACCTCTTGTCTCTCCTAAAAGCATATT ATGCTTTGAATGCACAACCAAGTGCAGAAGAGCTCTCAAAAATTGCTGATTCAGTAAACCTACCACTGGA TGTAGTAAAAAAGTGGTTTGAAAAGATGCAAGCTGGACAGATTTCAGTGCAGTCTTCTGAACCATCTTCT CCTGAACCAGGCAAAGTAAATATCCCTGCCAAGAACAATGATCAGCCTCAATCTGCAAATGCAAATGAAC CCCAGGACAGCACAGTAAATCTACAAAGTCCTTTGAAGATGACTAACTCCCCAGTTTTACCAGTGGGATC AACCACCAATGGTTCCAGAAGTAGTACACCATCCCCATCACCTCTAAACCTTTCCTCATCCAGAAATACA CAGGGTTACTTGTACACAGCTGAGGGTGCACAAGAAGAGCCACAAGTAGAACCTCTTGATCTTTCACTAC CAAAGCAACAGGGAGAATTATTAGAAAGGTCAACTATCACTAGTGTTTACCAGAACAGTGTTTATTCTGT CCAGGAAGAACCCTTGAACTTGTCTTGCGCAAAAAAGGAGCCACAAAAGGACAGTTGTGTTACAGACTCA GAACCAGTTGTAAATGTAATCCCACCAAGTGCCAACCCCATAAATATCGCTATACCTACAGTCACTGCCC AGTTACCCACAATCGTGGCCATTGCTGACCAGAACAGTGTTCCATGCTTAAGAGCGCTAGCTGCCAATAA GCAAACGATTCTGATTCCCCAGGTGGCATACACCTACTCAACTACGGTCAGCCCTGCAGTCCAAGAACCA CCCTTGAAAGTGATCCAGCCAAATGGAAATCAGGATGAAAGACAAGATACTAGCTCAGAAGGAGTATCAA ATGTAGAGGATCAGAATGACTCTGATTCTACACCGCCCAAAAAGAAAATGCGGAAGACAGAAAATGGAAT GTATGCTTGTGATTTGTGTGACAAGATATTCCAAAAGAGTAGTTCATTATTGAGACATAAATATGAACAC ACAGGTAAAAGACCTCATGAGTGTGGAATCTGTAAAAAGGCATTTAAACACAAACATCATTTGATTGAAC ACATGCGATTACATTCTGGAGAAAAGCCCTATCAATGTGACAAATGTGGAAAGCGCTTCTCACACTCTGG GTCTTATTCTCAACACATGAATCATCGCTACTCCTACTGTAAGAGAGAAGCGGAAGAACGTGACAGCACA GAGCAGGAAGAGGCAGGGCCTGAAATCCTCTCGAATGAGCACGTGGGTGCCAGGGCGTCTCCCTCACAGG GCGACTCGGACGAGAGAGAGAGTTTGACAAGGGAAGAGGATGAAGACAGTGAAAAAGAGGAAGAGGAGGA GGATAAAGAGATGGAAGAATTGCAGGAAGAAAAAGAATGTGAAAAACCACAAGGGGATGAGGAAGAGGAG GAGGAGGAGGAAGAAGTGGAAGAAGAAGAGGTAGAAGAGGCAGAGAATGAGGGAGAAGAAGCAAAAACTG AAGGTCTGATGAAGGATGACAGGGCTGAAAGTCAAGCAAGCAGCTTAGGACAAAAAGTAGGCGAGAGTAG TGAGCAAGTGTCTGAAGAAAAGACAAATGAAGCC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC226329 representing NM_001128128
Red=Cloning site Green=Tags(s) MKVTNYNTVVETNSDSDDEDKLHIVEEESVTDAADCEGVPEDDLPTDQTVLPGRSSEREGNAKNCWEDDT GKEGQEILGPEAQADEAGCTVKDDECESDAENEQNHDPNVEEFLQQQDTAVIFPEAPEEDQRQGTPEASG HDENGTPDAFSQLLTCPYCDRGYKRFTSLKEHIKYRHEKNEDNFSCSLCSYTFAYRTQLERHMTSHKSGR DQRHVTQSGCNRKFKCTECGKAFKYKHHLKEHLRIHSGEKPYECPNCKKRFSHSGSYSSHISSKKCISLI PVNGRPRTGLKTSQCSSPSLSASPGSPTRPQIRQKIENKPLQEQLSVNQIKTEPVDYEFKPIVVASGINC STPLQNGVFTGGGPLQATSSPQGMVQAVVLPTVGLVSPISINLSDIQNVLKVAVDGNVIRQVLENNQANL ASKEQETINASPIQQGGHSVISAISLPLVDQDGTTKIIINYSLEQPSQLQVVPQNLKKENPVATNSCKSE KLPEDLTVKSEKDKSFEGGVNDSTCLLCDDCPGDINALPELKHYDLKQPTQPPPLPAAEAEKPESSVSSA TGDGNLSPSQPPLKNLLSLLKAYYALNAQPSAEELSKIADSVNLPLDVVKKWFEKMQAGQISVQSSEPSS PEPGKVNIPAKNNDQPQSANANEPQDSTVNLQSPLKMTNSPVLPVGSTTNGSRSSTPSPSPLNLSSSRNT QGYLYTAEGAQEEPQVEPLDLSLPKQQGELLERSTITSVYQNSVYSVQEEPLNLSCAKKEPQKDSCVTDS EPVVNVIPPSANPINIAIPTVTAQLPTIVAIADQNSVPCLRALAANKQTILIPQVAYTYSTTVSPAVQEP PLKVIQPNGNQDERQDTSSEGVSNVEDQNDSDSTPPKKKMRKTENGMYACDLCDKIFQKSSSLLRHKYEH TGKRPHECGICKKAFKHKHHLIEHMRLHSGEKPYQCDKCGKRFSHSGSYSQHMNHRYSYCKREAEERDST EQEEAGPEILSNEHVGARASPSQGDSDERESLTREEDEDSEKEEEEEDKEMEELQEEKECEKPQGDEEEE EEEEEVEEEEVEEAENEGEEAKTEGLMKDDRAESQASSLGQKVGESSEQVSEEKTNEA TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001128128 |
| ORF Size | 3324 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001128128.3 |
| RefSeq ORF | 3327 bp |
| Locus ID | 6935 |
| UniProt ID | P37275 |
| Protein Families | Transcription Factors |
| MW | 121.9 kDa |
| Gene Summary | This gene encodes a zinc finger transcription factor. The encoded protein likely plays a role in transcriptional repression of interleukin 2. Mutations in this gene have been associated with posterior polymorphous corneal dystrophy-3 and late-onset Fuchs endothelial corneal dystrophy. Alternatively spliced transcript variants encoding different isoforms have been described.[provided by RefSeq, Mar 2010] |
Citations (2)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Nesfatin-1/Nucleobindin-2 enhances cell migration, invasion, and epithelial-mesenchymal transition via LKB1/AMPK/TORC1/ZEB1 pathways in colon cancer
,Kan, JY;Yen, MC;Wang, JY;Wu, DC;Chiu, YJ;Ho, YW;Kuo, PL;,
Oncotarget
,PubMed ID 27150059
[AREB6]
|
|
The Effect and Mechanism of miR?429 Regulating Proliferation,Migration and Invasion of Human U87 Glioma Cells
,CHEN Liang?yu,LIU Li?bo,LI Xin?xing,YU Bo,HONG Yang,ZHENG Jian,YU Qi,XUE Yi?xue,LIU Yun?hui,
Journal of China Medical University
[AREB6]
|
Documents
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| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC226329L1 | Lenti-ORF clone of ZEB1 (Myc-DDK-tagged)-Human zinc finger E-box binding homeobox 1 (ZEB1), transcript variant 1 |
CNY 13,968.00 |
|
| RC226329L2 | Lenti-ORF clone of ZEB1 (mGFP-tagged)-Human zinc finger E-box binding homeobox 1 (ZEB1), transcript variant 1 |
CNY 13,968.00 |
|
| RC226329L3 | Lenti-ORF clone of ZEB1 (Myc-DDK-tagged)-Human zinc finger E-box binding homeobox 1 (ZEB1), transcript variant 1 |
CNY 13,968.00 |
|
| RC226329L4 | Lenti-ORF clone of ZEB1 (mGFP-tagged)-Human zinc finger E-box binding homeobox 1 (ZEB1), transcript variant 1 |
CNY 13,968.00 |
|
| RG226329 | ZEB1 (tGFP-tagged) - Human zinc finger E-box binding homeobox 1 (ZEB1), transcript variant 1 |
CNY 13,168.00 |
|
| SC323255 | ZEB1 (untagged)-Human zinc finger E-box binding homeobox 1 (ZEB1), transcript variant 1 |
CNY 11,584.00 |
