POTEH (NM_001136213) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	A26C3; ACTBL1; CT104.7; POTE22
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC227124 ORF sequence, codon optimized. Due to the complexity of NM_001136213, the ORF clone is codon optimized for mammalian Expression. The nucleotide sequence differs from the reference sequence, yet the amino acid sequence remains identical. Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGTTGCAGAGGCTGGATCCATGCCGGCTGCATCCTCAGTCAAGAAGCCATTCGGCCTCCGCTCTAAGA TGGGTAAGTGGTGCAGGCATTGCTTCGCCTGGTGTCGGGGGTCAGGTAAAAGTAATGTGGGAACCTCAGG GGACCACGATGATTCAGCTATGAAGACGCTGCGGAGCAAGATGGGGAAGTGGTGTTGTCACTGCTTTCCT TGGTGTCGGGGCTCTGGCAAATCCAACGTGGGCACGTCTGGCGACCACGATGACAGTGCGATGAAGACTC TGAGGAGTAAGATGGGGAAATGGTGCTGCCATTGTTTTCCATGTTGTAGAGGCTCCGGCAAGAGCAACGT TGGCACCAGCGGGGACCACGACGACAGCGCCATGAAAACACTTCGGTCCAAGATGGGAAAGTGGTGCTGC CACTGCTTCCCATGTTGCAGAGGTAGCGGAAAGAACAAAGTGGGTCCTTGGGGAGACTACGACGACTCAG CCTTCATGGAACCGCGGTACCACGTCAGAAGGGAAGACCTGGACAAGCTCCACAGGGCTGCCTGGTGGGG TAAAGTGCCTCGGAAGGATCTGATTGTGATGCTTAAAGACACTGACATGAACAAAAAGGACAAGCAAAAA CGGACTGCTCTCCATCTGGCATCCGCCAACGGTAATAGCGAGGTCGTTAAGCTGCTTCTTGATAGGCGGT GTCAGCTGAACATCCTTGACAACAAGAAAAGAACTGCACTGACTAAGGCCGTGCAATGTCAAGAGGACGA GTGCGCCCTCATGTTGCTCGAACACGGGACCGATCCAAACATCCCAGACGAGTATGGGAATACAGCACTC CATTATGCTATCTACAATGAGGACAAGCTGATGGCGAAGGCACTGCTGCTGTATGGTGCCGACATTGAGT CCAAGAATAAGCATGGATTGACCCCCCTCCTCCTGGGTGTGCACGAACAGAAACAGCAGGTCGTGAAGTT TCTTATCAAGAAAAAGGCCAACCTGAATGCCCTGGATCGATACGGTAGAACTGCGCTGATTCTCGCGGTA TGTTGCGGAAGCGCTAGCATTGTATCTCTTCTGCTGGAGCAGAATATCGATGTGTCCTCCCAGGATCTCA GCGGACAAACCGCTCGGGAATATGCAGTCAGCTCTAGACACAACGTAATCTGCCAGCTTCTGTCCGATTA TAAAGAAAAGCAGATCCTCAAAGTCTCATCCGAGAACTCTAACCCCGAGCAGGATCTGAAGCTGACCAGC GAAGAGGAGTCTCAGCGCCTGAAAGGCTCTGAGAACAGCCAACCTGAGGAAATGTCACAGGAACCAGAAA TTAACAAGGGCGGAGACAGGAAAGTGGAAGAAGAAATGAAGAAACATGGCTCCACCCATATGGGGTTTCC GGAGAACCTGACCAATGGAGCCACGGCCGATAATGGAGATGACGGATTGATCCCCCCCCGAAAGAGCCGC ACCCCCGAGTCCCAGCAATTCCCCGACACCGAAAATGAACAGTACCACTCCGACGAACAGAACGATACTC AGAAGCAACTGAGTGAGGAGCAGAATACTGGCATCCTTCAGGACGAAATCCTGATCCACGAGGAGAAACA AATTGAGGTAGCCGAGAATGAATTT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC227124 representing NM_001136213 Red=Cloning site Green=Tags(s) MVAEAGSMPAASSVKKPFGLRSKMGKWCRHCFAWCRGSGKSNVGTSGDHDDSAMKTLRSKMGKWCCHCFP WCRGSGKSNVGTSGDHDDSAMKTLRSKMGKWCCHCFPCCRGSGKSNVGTSGDHDDSAMKTLRSKMGKWCC HCFPCCRGSGKNKVGPWGDYDDSAFMEPRYHVRREDLDKLHRAAWWGKVPRKDLIVMLKDTDMNKKDKQK RTALHLASANGNSEVVKLLLDRRCQLNILDNKKRTALTKAVQCQEDECALMLLEHGTDPNIPDEYGNTAL HYAIYNEDKLMAKALLLYGADIESKNKHGLTPLLLGVHEQKQQVVKFLIKKKANLNALDRYGRTALILAV CCGSASIVSLLLEQNIDVSSQDLSGQTAREYAVSSRHNVICQLLSDYKEKQILKVSSENSNPEQDLKLTS EEESQRLKGSENSQPEEMSQEPEINKGGDRKVEEEMKKHGSTHMGFPENLTNGATADNGDDGLIPPRKSR TPESQQFPDTENEQYHSDEQNDTQKQLSEEQNTGILQDEILIHEEKQIEVAENEF TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001136213
ORF Size	1635 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001136213.1, NP_001129685.1
RefSeq Size	2037 bp
RefSeq ORF	1638 bp
Locus ID	23784
UniProt ID	Q6S545
MW	61 kDa

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