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ALS (IGFALS) (NM_001146006) Human Tagged ORF Clone
CAT＃: RC228516
- TrueORF®
IGFALS (Myc-DDK-tagged)-Human insulin-like growth factor binding protein, acid labile subunit (IGFALS), transcript variant 1
ORF Plasmid: tGFP
"NM_001146006" in other vectors (4)
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CNY 4,784.00
CNY 5,700.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | ACLSD; ALS |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC228516 representing NM_001146006
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCCTGAGGAAAGCGGGTGACCTCGAGCCTCAGTTCACACCTGAGCGCCGCTTCCGGCTGTGCTGGT ATCAGGCTCACTCGGGCAGAGCCCTCCTCGGGCCGCCTCCCCAAGCCTCTCCCCCCGCAGGAGGCCTGGC CCTGGCGCTGCTGCTGCTGTCCTGGGTGGCACTGGGCCCCCGCAGCCTGGAGGGAGCAGACCCCGGAACG CCGGGGGAAGCCGAGGGCCCAGCGTGCCCGGCCGCCTGTGTCTGCAGCTACGATGACGACGCGGATGAGC TCAGCGTCTTCTGCAGCTCCAGGAACCTCACGCGCCTGCCTGATGGAGTCCCGGGCGGCACCCAAGCCCT GTGGCTGGACGGCAACAACCTCTCGTCCGTCCCCCCGGCAGCCTTCCAGAACCTCTCCAGCCTGGGCTTC CTCAACCTGCAGGGCGGCCAGCTGGGCAGCCTGGAGCCACAGGCGCTGCTGGGCCTAGAGAACCTGTGCC ACCTGCACCTGGAGCGGAACCAGCTGCGCAGCCTGGCACTCGGCACGTTTGCACACACGCCCGCGCTGGC CTCGCTCGGCCTCAGCAACAACCGTCTGAGCAGGCTGGAGGACGGGCTCTTCGAGGGCCTCGGCAGCCTC TGGGACCTCAACCTCGGCTGGAATAGCCTGGCGGTGCTCCCCGATGCGGCGTTCCGCGGCCTGGGCAGCC TGCGCGAGCTGGTGCTGGCGGGCAACAGGCTGGCCTACCTGCAGCCCGCGCTCTTCAGCGGCCTGGCCGA GCTCCGGGAGCTGGACCTGAGCAGGAACGCGCTGCGGGCCATCAAGGCAAACGTGTTCGTGCAGCTGCCC CGGCTCCAGAAACTCTACCTGGACCGCAACCTCATCGCTGCCGTGGCCCCGGGCGCCTTCCTGGGCCTGA AGGCGCTGCGATGGCTGGACCTGTCCCACAACCGCGTGGCTGGCCTCCTGGAGGACACGTTCCCCGGTCT GCTGGGCCTGCGTGTGCTGCGGCTGTCCCACAACGCCATCGCCAGCCTGCGGCCCCGCACCTTCAAGGAC CTGCACTTCCTGGAGGAGCTGCAGCTGGGCCACAACCGCATCCGGCAGCTGGCTGAGCGCAGCTTTGAGG GCCTGGGGCAGCTTGAGGTGCTCACGCTAGACCACAACCAGCTCCAGGAGGTCAAGGCGGGCGCTTTCCT CGGCCTCACCAACGTGGCGGTCATGAACCTCTCTGGGAACTGTCTCCGGAACCTTCCGGAGCAGGTGTTC CGGGGCCTGGGCAAGCTGCACAGCCTGCACCTGGAGGGCAGCTGCCTGGGACGCATCCGCCCGCACACCT TCACCGGCCTCTCGGGGCTCCGCCGACTCTTCCTCAAGGACAACGGCCTCGTGGGCATTGAGGAGCAGAG CCTGTGGGGGCTGGCGGAGCTGCTGGAGCTCGACCTGACCTCCAACCAGCTCACGCACCTGCCCCACCGC CTCTTCCAGGGCCTGGGCAAGCTGGAGTACCTGCTGCTCTCCCGCAACCGCCTGGCAGAGCTGCCGGCGG ACGCCCTGGGCCCCCTGCAGCGGGCCTTCTGGCTGGACGTCTCGCACAACCGCCTGGAGGCATTGCCCAA CAGCCTCTTGGCACCACTGGGGCGGCTGCGCTACCTCAGCCTCAGGAACAACTCACTGCGGACCTTCACG CCGCAGCCCCCGGGCCTGGAGCGCCTGTGGCTGGAGGGTAACCCCTGGGACTGTGGCTGCCCTCTCAAGG CGCTGCGGGACTTCGCCCTGCAGAACCCCAGTGCTGTGCCCCGCTTCGTCCAGGCCATCTGTGAGGGGGA CGATTGCCAGCCGCCCGCGTACACCTACAACAACATCACCTGTGCCAGCCCGCCCGAGGTCGTGGGGCTC GACCTGCGGGACCTCAGCGAGGCCCACTTTGCTCCCTGC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC228516 representing NM_001146006
Red=Cloning site Green=Tags(s) MALRKAGDLEPQFTPERRFRLCWYQAHSGRALLGPPPQASPPAGGLALALLLLSWVALGPRSLEGADPGT PGEAEGPACPAACVCSYDDDADELSVFCSSRNLTRLPDGVPGGTQALWLDGNNLSSVPPAAFQNLSSLGF LNLQGGQLGSLEPQALLGLENLCHLHLERNQLRSLALGTFAHTPALASLGLSNNRLSRLEDGLFEGLGSL WDLNLGWNSLAVLPDAAFRGLGSLRELVLAGNRLAYLQPALFSGLAELRELDLSRNALRAIKANVFVQLP RLQKLYLDRNLIAAVAPGAFLGLKALRWLDLSHNRVAGLLEDTFPGLLGLRVLRLSHNAIASLRPRTFKD LHFLEELQLGHNRIRQLAERSFEGLGQLEVLTLDHNQLQEVKAGAFLGLTNVAVMNLSGNCLRNLPEQVF RGLGKLHSLHLEGSCLGRIRPHTFTGLSGLRRLFLKDNGLVGIEEQSLWGLAELLELDLTSNQLTHLPHR LFQGLGKLEYLLLSRNRLAELPADALGPLQRAFWLDVSHNRLEALPNSLLAPLGRLRYLSLRNNSLRTFT PQPPGLERLWLEGNPWDCGCPLKALRDFALQNPSAVPRFVQAICEGDDCQPPAYTYNNITCASPPEVVGL DLRDLSEAHFAPC TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001146006 |
| ORF Size | 1929 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001146006.2 |
| RefSeq ORF | 1932 bp |
| Locus ID | 3483 |
| UniProt ID | P35858 |
| Protein Families | Druggable Genome, Secreted Protein |
| MW | 70.24 kDa |
| Gene Summary | The protein encoded by this gene is a serum protein that binds insulin-like growth factors, increasing their half-life and their vascular localization. Production of the encoded protein, which contains twenty leucine-rich repeats, is stimulated by growth hormone. Defects in this gene are a cause of acid-labile subunit deficiency, which maifests itself in a delayed and slow puberty. Three transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Mar 2009] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC228516L3 | Lenti-ORF clone of IGFALS (Myc-DDK-tagged)-Human insulin-like growth factor binding protein, acid labile subunit (IGFALS), transcript variant 1 |
CNY 7,600.00 |
|
| RC228516L4 | Lenti-ORF clone of IGFALS (mGFP-tagged)-Human insulin-like growth factor binding protein, acid labile subunit (IGFALS), transcript variant 1 |
CNY 7,600.00 |
|
| RG228516 | IGFALS (tGFP-tagged) - Human insulin-like growth factor binding protein, acid labile subunit (IGFALS), transcript variant 1 |
CNY 6,270.00 |
|
| SC327151 | IGFALS (untagged)-Human insulin-like growth factor binding protein acid labile subunit (IGFALS) transcript variant 1 |
CNY 10,360.00 |
