Nav1.5 (SCN5A) (NM_001160161) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	CDCD2; CMD1E; CMPD2; HB1; HB2; HBBD; HH1; ICCD; IVF; LQT3; Nav1.5; PFHB1; SSS1; VF1
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC228704 representing NM_001160161 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCAAACTTCCTATTACCTCGGGGCACCAGCAGCTTCCGCAGGTTCACACGGGAGTCCCTGGCAGCCA TCGAGAAGCGCATGGCAGAGAAGCAAGCCCGCGGCTCAACCACCTTGCAGGAGAGCCGAGAGGGGCTGCC CGAGGAGGAGGCTCCCCGGCCCCAGCTGGACCTGCAGGCCTCCAAAAAGCTGCCAGATCTCTATGGCAAT CCACCCCAAGAGCTCATCGGAGAGCCCCTGGAGGACCTGGACCCCTTCTATAGCACCCAAAAGACTTTCA TCGTACTGAATAAAGGCAAGACCATCTTCCGGTTCAGTGCCACCAACGCCTTGTATGTCCTCAGTCCCTT CCACCCCATCCGGAGAGCGGCTGTGAAGATTCTGGTTCACTCGCTCTTCAACATGCTCATCATGTGCACC ATCCTCACCAACTGCGTGTTCATGGCCCAGCACGACCCTCCACCCTGGACCAAGTATGTCGAGTACACCT TCACCGCCATTTACACCTTTGAGTCTCTGGTCAAGATTCTGGCTCGAGGCTTCTGCCTGCACGCGTTCAC TTTCCTTCGGGACCCATGGAACTGGCTGGACTTTAGTGTGATTATCATGGCGTATGTATCAGAAAATATA AAACTAGGCAATTTGTCGGCTCTTCGAACTTTCAGAGTCCTGAGAGCTCTAAAAACTATTTCAGTTATCC CAGGGCTGAAGACCATCGTGGGGGCCCTGATCCAGTCTGTGAAGAAGCTGGCTGATGTGATGGTCCTCAC AGTCTTCTGCCTCAGCGTCTTTGCCCTCATCGGCCTGCAGCTCTTCATGGGCAACCTAAGGCACAAGTGC GTGCGCAACTTCACAGCGCTCAACGGCACCAACGGCTCCGTGGAGGCCGACGGCTTGGTCTGGGAATCCC TGGACCTTTACCTCAGTGATCCAGAAAATTACCTGCTCAAGAACGGCACCTCTGATGTGTTACTGTGTGG GAACAGCTCTGACGCTGGGACATGTCCGGAGGGCTACCGGTGCCTAAAGGCAGGCGAGAACCCCGACCAC GGCTACACCAGCTTCGATTCCTTTGCCTGGGCCTTTCTTGCACTCTTCCGCCTGATGACGCAGGACTGCT GGGAGCGCCTCTATCAGCAGACCCTCAGGTCCGCAGGGAAGATCTACATGATCTTCTTCATGCTTGTCAT CTTCCTGGGGTCCTTCTACCTGGTGAACCTGATCCTGGCCGTGGTCGCAATGGCCTATGAGGAGCAAAAC CAAGCCACCATCGCTGAGACCGAGGAGAAGGAAAAGCGCTTCCAGGAGGCCATGGAAATGCTCAAGAAAG AACACGAGGCCCTCACCATCAGGGGTGTGGATACCGTGTCCCGTAGCTCCTTGGAGATGTCCCCTTTGGC CCCAGTAAACAGCCATGAGAGAAGAAGCAAGAGGAGAAAACGGATGTCTTCAGGAACTGAGGAGTGTGGG GAGGACAGGCTCCCCAAGTCTGACTCAGAAGATGGTCCCAGAGCAATGAATCATCTCAGCCTCACCCGTG GCCTCAGCAGGACTTCTATGAAGCCACGTTCCAGCCGCGGGAGCATTTTCACCTTTCGCAGGCGAGACCT GGGTTCTGAAGCAGATTTTGCAGATGATGAAAACAGCACAGCGGGGGAGAGCGAGAGCCACCACACATCA CTGCTGGTGCCCTGGCCCCTGCGCCGGACCAGTGCCCAGGGACAGCCCAGTCCCGGAACCTCGGCTCCTG GCCACGCCCTCCATGGCAAAAAGAACAGCACTGTGGACTGCAATGGGGTGGTCTCATTACTGGGGGCAGG CGACCCAGAGGCCACATCCCCAGGAAGCCACCTCCTCCGCCCTGTGATGCTAGAGCACCCGCCAGACACG ACCACGCCATCGGAGGAGCCAGGCGGGCCCCAGATGCTGACCTCCCAGGCTCCGTGTGTAGATGGCTTCG AGGAGCCAGGAGCACGGCAGCGGGCCCTCAGCGCAGTCAGCGTCCTCACCAGCGCACTGGAAGAGTTAGA GGAGTCTCGCCACAAGTGTCCACCATGCTGGAACCGTCTCGCCCAGCGCTACCTGATCTGGGAGTGCTGC CCGCTGTGGATGTCCATCAAGCAGGGAGTGAAGTTGGTGGTCATGGACCCGTTTACTGACCTCACCATCA CTATGTGCATCGTACTCAACACACTCTTCATGGCGCTGGAGCACTACAACATGACAAGTGAATTCGAGGA GATGCTGCAGGTCGGAAACCTGGTCTTCACAGGGATTTTCACAGCAGAGATGACCTTCAAGATCATTGCC CTCGACCCCTACTACTACTTCCAACAGGGCTGGAACATCTTCGACAGCATCATCGTCATCCTTAGCCTCA TGGAGCTGGGCCTGTCCCGCATGAGCAACTTGTCGGTGCTGCGCTCCTTCCGCCTGCTGCGGGTCTTCAA GCTGGCCAAATCATGGCCCACCCTGAACACACTCATCAAGATCATCGGGAACTCAGTGGGGGCACTGGGG AACCTGACACTGGTGCTAGCCATCATCGTGTTCATCTTTGCTGTGGTGGGCATGCAGCTCTTTGGCAAGA ACTACTCGGAGCTGAGGGACAGCGACTCAGGCCTGCTGCCTCGCTGGCACATGATGGACTTCTTTCATGC CTTCCTCATCATCTTCCGCATCCTCTGTGGAGAGTGGATCGAGACCATGTGGGACTGCATGGAGGTGTCG GGGCAGTCATTATGCCTGCTGGTCTTCTTGCTTGTTATGGTCATTGGCAACCTTGTGGTCCTGAATCTCT TCCTGGCCTTGCTGCTCAGCTCCTTCAGTGCAGACAACCTCACAGCCCCTGATGAGGACAGAGAGATGAA CAACCTCCAGCTGGCCCTGGCCCGCATCCAGAGGGGCCTGCGCTTTGTCAAGCGGACCACCTGGGATTTC TGCTGTGGTCTCCTGCGGCAGCGGCCTCAGAAGCCCGCAGCCCTTGCCGCCCAGGGCCAGCTGCCCAGCT GCATTGCCACCCCCTACTCCCCGCCACCCCCAGAGACGGAGAAGGTGCCTCCCACCCGCAAGGAAACACG GTTTGAGGAAGGCGAGCAACCAGGCCAGGGCACCCCCGGGGATCCAGAGCCCGTGTGTGTGCCCATCGCT GTGGCCGAGTCAGACACAGATGACCAAGAAGAAGATGAGGAGAACAGCCTGGGCACGGAGGAGGAGTCCA GCAAGCAGACCCCAGAGGACAGTTGCTCCGAGGGCAGCACAGCAGACATGACCAACACCGCTGAGCTCCT GGAGCAGATCCCTGACCTCGGCCAGGATGTCAAGGACCCAGAGGACTGCTTCACTGAAGGCTGTGTCCGG CGCTGTCCCTGCTGTGCGGTGGACACCACACAGGCCCCAGGGAAGGTCTGGTGGCGGTTGCGCAAGACCT GCTACCACATCGTGGAGCACAGCTGGTTCGAGACATTCATCATCTTCATGATCCTACTCAGCAGTGGAGC GCTGGCCTTCGAGGACATCTACCTAGAGGAGCGGAAGACCATCAAGGTTCTGCTTGAGTATGCCGACAAG ATGTTCACATATGTCTTCGTGCTGGAGATGCTGCTCAAGTGGGTGGCCTACGGCTTCAAGAAGTACTTCA CCAATGCCTGGTGCTGGCTCGACTTCCTCATCGTAGACGTCTCTCTGGTCAGCCTGGTGGCCAACACCCT GGGCTTTGCCGAGATGGGCCCCATCAAGTCACTGCGGACGCTGCGTGCACTCCGTCCTCTGAGAGCTCTG TCACGATTTGAGGGCATGAGGGTGGTGGTCAATGCCCTGGTGGGCGCCATCCCGTCCATCATGAACGTCC TCCTCGTCTGCCTCATCTTCTGGCTCATCTTCAGCATCATGGGCGTGAACCTCTTTGCGGGGAAGTTTGG GAGGTGCATCAACCAGACAGAGGGAGACTTGCCTTTGAACTACACCATCGTGAACAACAAGAGCCAGTGT GAGTCCTTGAACTTGACCGGAGAATTGTACTGGACCAAGGTGAAAGTCAACTTTGACAACGTGGGGGCCG GGTACCTGGCCCTTCTGCAGGTGGCAACATTTAAAGGCTGGATGGACATTATGTATGCAGCTGTGGACTC CAGGGGGTATGAAGAGCAGCCTCAGTGGGAATACAACCTCTACATGTACATCTATTTTGTCATTTTCATC ATCTTTGGGTCTTTCTTCACCCTGAACCTCTTTATTGGTGTCATCATTGACAACTTCAACCAACAGAAGA AAAAGTTAGGGGGCCAGGACATCTTCATGACAGAGGAGCAGAAGAAGTACTACAATGCCATGAAGAAGCT GGGCTCCAAGAAGCCCCAGAAGCCCATCCCACGGCCCCTGAACAAGTACCAGGGCTTCATATTCGACATT GTGACCAAGCAGGCCTTTGACGTCACCATCATGTTTCTGATCTGCTTGAATATGGTGACCATGATGGTGG AGACAGATGACCAAAGTCCTGAGAAAATCAACATCTTGGCCAAGATCAACCTGCTCTTTGTGGCCATCTT CACAGGCGAGTGTATTGTCAAGCTGGCTGCCCTGCGCCACTACTACTTCACCAACAGCTGGAATATCTTC GACTTCGTGGTTGTCATCCTCTCCATCGTGGGCACTGTGCTCTCGGACATCATCCAGAAGTACTTCTTCT CCCCGACGCTCTTCCGAGTCATCCGCCTGGCCCGAATAGGCCGCATCCTCAGACTGATCCGAGGGGCCAA GGGGATCCGCACGCTGCTCTTTGCCCTCATGATGTCCCTGCCTGCCCTCTTCAACATCGGGCTGCTGCTC TTCCTCGTCATGTTCATCTACTCCATCTTTGGCATGGCCAACTTCGCTTATGTCAAGTGGGAGGCTGGCA TCGACGACATGTTCAACTTCCAGACCTTCGCCAACAGCATGCTGTGCCTCTTCCAGATCACCACGTCGGC CGGCTGGGATGGCCTCCTCAGCCCCATCCTCAACACTGGGCCGCCCTACTGCGACCCCACTCTGCCCAAC AGCAATGGCTCTCGGGGGGACTGCGGGAGCCCAGCCGTGGGCATCCTCTTCTTCACCACCTACATCATCA TCTCCTTCCTCATCGTGGTCAACATGTACATTGCCATCATCCTGGAGAACTTCAGCGTGGCCACGGAGGA GAGCACCGAGCCCCTGAGTGAGGACGACTTCGATATGTTCTATGAGATCTGGGAGAAATTTGACCCAGAG GCCACTCAGTTTATTGAGTATTCGGTCCTGTCTGACTTTGCCGATGCCCTGTCTGAGCCACTCCGTATCG CCAAGCCCAACCAGATAAGCCTCATCAACATGGACCTGCCCATGGTGAGTGGGGACCGCATCCATTGCAT GGACATTCTCTTTGCCTTCACCAAAAGGGTCCTGGGGGAGTCTGGGGAGATGGACGCCCTGAAGATCCAG ATGGAGGAGAAGTTCATGGCAGCCAACCCATCCAAGATCTCCTACGAGCCCATCACCACCACACTCCGGC GCAAGCACGAAGAGGTGTCGGCCATGGTTATCCAGAGAGCCTTCCGCAGGCACCTGCTGCAACGCTCTTT GAAGCATGCCTCCTTCCTCTTCCGTCAGCAGGCGGGCAGCGGCCTCTCCGAAGAGGATGCCCCTGAGCGA GAGGGCCTCATCGCCTACGTGATGAGTGAGAACTTCTCCCGACCCCTTGGCCCACCCTCCAGCTCCTCCA TCTCCTCCACTTCCTTCCCACCCTCCTATGACAGTGTCACTAGAGCCACCAGCGATAACCTCCAGGTGCG GGGGTCTGACTACAGCCACAGTGAAGATCTCGCCGACTTCCCCCCTTCTCCGGACAGGGACCGTGAGTCC ATCGTG AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC TGGATTACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC228704 representing NM_001160161 Red=Cloning site Green=Tags(s) MANFLLPRGTSSFRRFTRESLAAIEKRMAEKQARGSTTLQESREGLPEEEAPRPQLDLQASKKLPDLYGN PPQELIGEPLEDLDPFYSTQKTFIVLNKGKTIFRFSATNALYVLSPFHPIRRAAVKILVHSLFNMLIMCT ILTNCVFMAQHDPPPWTKYVEYTFTAIYTFESLVKILARGFCLHAFTFLRDPWNWLDFSVIIMAYVSENI KLGNLSALRTFRVLRALKTISVIPGLKTIVGALIQSVKKLADVMVLTVFCLSVFALIGLQLFMGNLRHKC VRNFTALNGTNGSVEADGLVWESLDLYLSDPENYLLKNGTSDVLLCGNSSDAGTCPEGYRCLKAGENPDH GYTSFDSFAWAFLALFRLMTQDCWERLYQQTLRSAGKIYMIFFMLVIFLGSFYLVNLILAVVAMAYEEQN QATIAETEEKEKRFQEAMEMLKKEHEALTIRGVDTVSRSSLEMSPLAPVNSHERRSKRRKRMSSGTEECG EDRLPKSDSEDGPRAMNHLSLTRGLSRTSMKPRSSRGSIFTFRRRDLGSEADFADDENSTAGESESHHTS LLVPWPLRRTSAQGQPSPGTSAPGHALHGKKNSTVDCNGVVSLLGAGDPEATSPGSHLLRPVMLEHPPDT TTPSEEPGGPQMLTSQAPCVDGFEEPGARQRALSAVSVLTSALEELEESRHKCPPCWNRLAQRYLIWECC PLWMSIKQGVKLVVMDPFTDLTITMCIVLNTLFMALEHYNMTSEFEEMLQVGNLVFTGIFTAEMTFKIIA LDPYYYFQQGWNIFDSIIVILSLMELGLSRMSNLSVLRSFRLLRVFKLAKSWPTLNTLIKIIGNSVGALG NLTLVLAIIVFIFAVVGMQLFGKNYSELRDSDSGLLPRWHMMDFFHAFLIIFRILCGEWIETMWDCMEVS GQSLCLLVFLLVMVIGNLVVLNLFLALLLSSFSADNLTAPDEDREMNNLQLALARIQRGLRFVKRTTWDF CCGLLRQRPQKPAALAAQGQLPSCIATPYSPPPPETEKVPPTRKETRFEEGEQPGQGTPGDPEPVCVPIA VAESDTDDQEEDEENSLGTEEESSKQTPEDSCSEGSTADMTNTAELLEQIPDLGQDVKDPEDCFTEGCVR RCPCCAVDTTQAPGKVWWRLRKTCYHIVEHSWFETFIIFMILLSSGALAFEDIYLEERKTIKVLLEYADK MFTYVFVLEMLLKWVAYGFKKYFTNAWCWLDFLIVDVSLVSLVANTLGFAEMGPIKSLRTLRALRPLRAL SRFEGMRVVVNALVGAIPSIMNVLLVCLIFWLIFSIMGVNLFAGKFGRCINQTEGDLPLNYTIVNNKSQC ESLNLTGELYWTKVKVNFDNVGAGYLALLQVATFKGWMDIMYAAVDSRGYEEQPQWEYNLYMYIYFVIFI IFGSFFTLNLFIGVIIDNFNQQKKKLGGQDIFMTEEQKKYYNAMKKLGSKKPQKPIPRPLNKYQGFIFDI VTKQAFDVTIMFLICLNMVTMMVETDDQSPEKINILAKINLLFVAIFTGECIVKLAALRHYYFTNSWNIF DFVVVILSIVGTVLSDIIQKYFFSPTLFRVIRLARIGRILRLIRGAKGIRTLLFALMMSLPALFNIGLLL FLVMFIYSIFGMANFAYVKWEAGIDDMFNFQTFANSMLCLFQITTSAGWDGLLSPILNTGPPYCDPTLPN SNGSRGDCGSPAVGILFFTTYIIISFLIVVNMYIAIILENFSVATEESTEPLSEDDFDMFYEIWEKFDPE ATQFIEYSVLSDFADALSEPLRIAKPNQISLINMDLPMVSGDRIHCMDILFAFTKRVLGESGEMDALKIQ MEEKFMAANPSKISYEPITTTLRRKHEEVSAMVIQRAFRRHLLQRSLKHASFLFRQQAGSGLSEEDAPER EGLIAYVMSENFSRPLGPPSSSSISSTSFPPSYDSVTRATSDNLQVRGSDYSHSEDLADFPPSPDRDRES IV SGPTRTRRLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-RsrII      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001160161
ORF Size	5886 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001160161.2
RefSeq ORF	5889 bp
Locus ID	6331
UniProt ID	Q14524
Protein Families	Druggable Genome, Ion Channels: Sodium, Transmembrane
MW	221.1 kDa
Gene Summary	The protein encoded by this gene is an integral membrane protein and tetrodotoxin-resistant voltage-gated sodium channel subunit. This protein is found primarily in cardiac muscle and is responsible for the initial upstroke of the action potential in an electrocardiogram. Defects in this gene are a cause of long QT syndrome type 3 (LQT3), an autosomal dominant cardiac disease. Alternative splicing results in several transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]

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