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Glucose 6 phosphate isomerase (GPI) (NM_001184722) Human Tagged ORF Clone
CAT＃: RC230292
GPI (Myc-DDK-tagged)-Human glucose-6-phosphate isomerase (GPI), transcript variant 1
ORF Plasmid: tGFP
"NM_001184722" in other vectors (6)
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CNY 6,352.00
| Cited in 2 publications. |
Product images
CNY 300.00
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | AMF; GNPI; NLK; PGI; PHI; SA-36; SA36 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC230292 representing NM_001184722
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGTAGCTCTCTGCAGCCTCCAACACCTGGGCTCCAGTGATCCCCGGGCTCTGCCCACCCTCCCCACTG CCACTTCCGGGCAGAGGCCAGCAAAGCGGCGGCGCAAGAGTCCCGCCATGGCCGCTCTCACCCGGGACCC CCAGTTCCAGAAGCTGCAGCAATGGTACCGCGAGCACCGCTCCGAGCTGAACCTGCGCCGCCTCTTCGAT GCCAACAAGGACCGCTTCAACCACTTCAGCTTGACCCTCAACACCAACCATGGGCATATCCTGGTGGATT ACTCCAAGAACCTGGTGACGGAGGACGTGATGCGGATGCTGGTGGACTTGGCCAAGTCCAGGGGCGTGGA GGCCGCCCGGGAGCGGATGTTCAATGGTGAGAAGATCAACTACACCGAGGGTCGAGCCGTGCTGCACGTG GCTCTGCGGAACCGGTCAAACACACCCATCCTGGTAGACGGCAAGGATGTGATGCCAGAGGTCAACAAGG TTCTGGACAAGATGAAGTCTTTCTGCCAGGGACCCCTCATGGTGACTGAAGCCCTTAAGCCATACTCTTC AGGAGGTCCCCGCGTCTGGTATGTCTCCAACATTGATGGAACTCACATTGCCAAAACCCTGGCCCAGCTG AACCCCGAGTCCTCCCTGTTCATCATTGCCTCCAAGACCTTTACTACCCAGGAGACCATCACGAATGCAG AGACGGCGAAGGAGTGGTTTCTCCAGGCGGCCAAGGATCCTTCTGCAGTGGCGAAGCACTTTGTTGCCCT GTCTACTAACACAACCAAAGTGAAGGAGTTTGGAATTGACCCTCAAAACATGTTCGAGTTCTGGGATTGG GTGGGAGGACGCTACTCGCTGTGGTCGGCCATCGGACTCTCCATTGCCCTGCACGTGGGTTTTGACAACT TCGAGCAGCTGCTCTCGGGGGCTCACTGGATGGACCAGCACTTCCGCACGACGCCCCTGGAGAAGAACGC CCCCGTCTTGCTGGCCCTGCTGGGTATCTGGTACATCAACTGCTTTGGGTGTGAGACACACGCCATGCTG CCCTATGACCAGTACCTGCACCGCTTTGCTGCGTACTTCCAGCAGGGCGACATGGAGTCCAATGGGAAAT ACATCACCAAATCTGGAACCCGTGTGGACCACCAGACAGGCCCCATTGTGTGGGGGGAGCCAGGGACCAA TGGCCAGCATGCTTTTTACCAGCTCATCCACCAAGGCACCAAGATGATACCCTGTGACTTCCTCATCCCG GTCCAGACCCAGCACCCCATACGGAAGGGTCTGCATCACAAGATCCTCCTGGCCAACTTCTTGGCCCAGA CAGAGGCCCTGATGAGGGGAAAATCGACGGAGGAGGCCCGAAAGGAGCTCCAGGCTGCGGGCAAGAGTCC AGAGGACCTTGAGAGGCTGCTGCCACATAAGGTCTTTGAAGGAAATCGCCCAACCAACTCTATTGTGTTC ACCAAGCTCACACCATTCATGCTTGGAGCCTTGGTCGCCATGTATGAGCACAAGATCTTCGTTCAGGGCA TCATCTGGGACATCAACAGCTTTGACCAGTGGGGAGTGGAGCTGGGAAAGCAGCTGGCTAAGAAAATAGA GCCTGAGCTTGATGGCAGTGCTCAAGTGACCTCTCACGACGCTTCTACCAATGGGCTCATCAACTTCATC AAGCAGCAGCGCGAGGCCAGAGTCCAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC230292 representing NM_001184722
Red=Cloning site Green=Tags(s) MVALCSLQHLGSSDPRALPTLPTATSGQRPAKRRRKSPAMAALTRDPQFQKLQQWYREHRSELNLRRLFD ANKDRFNHFSLTLNTNHGHILVDYSKNLVTEDVMRMLVDLAKSRGVEAARERMFNGEKINYTEGRAVLHV ALRNRSNTPILVDGKDVMPEVNKVLDKMKSFCQGPLMVTEALKPYSSGGPRVWYVSNIDGTHIAKTLAQL NPESSLFIIASKTFTTQETITNAETAKEWFLQAAKDPSAVAKHFVALSTNTTKVKEFGIDPQNMFEFWDW VGGRYSLWSAIGLSIALHVGFDNFEQLLSGAHWMDQHFRTTPLEKNAPVLLALLGIWYINCFGCETHAML PYDQYLHRFAAYFQQGDMESNGKYITKSGTRVDHQTGPIVWGEPGTNGQHAFYQLIHQGTKMIPCDFLIP VQTQHPIRKGLHHKILLANFLAQTEALMRGKSTEEARKELQAAGKSPEDLERLLPHKVFEGNRPTNSIVF TKLTPFMLGALVAMYEHKIFVQGIIWDINSFDQWGVELGKQLAKKIEPELDGSAQVTSHDASTNGLINFI KQQREARVQ TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001184722 |
| ORF Size | 1707 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001184722.1, NP_001171651.1 |
| RefSeq ORF | 1710 bp |
| Locus ID | 2821 |
| UniProt ID | P06744 |
| Protein Families | Druggable Genome |
| Protein Pathways | Amino sugar and nucleotide sugar metabolism, Glycolysis / Gluconeogenesis, Metabolic pathways, Pentose phosphate pathway, Starch and sucrose metabolism |
| MW | 64.8 kDa |
| Gene Summary | This gene encodes a member of the glucose phosphate isomerase protein family. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. In the cytoplasm, the gene product functions as a glycolytic enzyme (glucose-6-phosphate isomerase) that interconverts glucose-6-phosphate and fructose-6-phosphate. Extracellularly, the encoded protein (also referred to as neuroleukin) functions as a neurotrophic factor that promotes survival of skeletal motor neurons and sensory neurons, and as a lymphokine that induces immunoglobulin secretion. The encoded protein is also referred to as autocrine motility factor based on an additional function as a tumor-secreted cytokine and angiogenic factor. Defects in this gene are the cause of nonspherocytic hemolytic anemia and a severe enzyme deficiency can be associated with hydrops fetalis, immediate neonatal death and neurological impairment. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2016] |
Citations (2)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
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Severe fever with thrombocytopenia syndrome virus (SFTSV)-host interactome screen identifies viral nucleoprotein-associated host factors as potential antiviral targets
,null,
Computational and Structural Biotechnology Journal
,PubMed ID 34712400
[Glucose 6 phosphate isomerase]
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Interplay between hypoxia and androgen controls a metabolic switch conferring resistance to androgen/AR-targeted therapy
,Geng, H;Xue, C;Mendonca, J;Sun, XX;Liu, Q;Reardon, PN;Chen, Y;Qian, K;Hua, V;Chen, A;Pan, F;Yuan, J;Dang, S;Beer, TM;Dai, MS;Kachhap, SK;Qian, DZ;,
Nat Commun
,PubMed ID 30478344
[Glucose 6 phosphate isomerase]
|
Documents
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| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC230292L1 | Lenti-ORF clone of GPI (Myc-DDK-tagged)-Human glucose-6-phosphate isomerase (GPI), transcript variant 1 |
CNY 8,752.00 |
|
| RC230292L2 | Lenti-ORF clone of GPI (mGFP-tagged)-Human glucose-6-phosphate isomerase (GPI), transcript variant 1 |
CNY 8,752.00 |
|
| RC230292L3 | Lenti-ORF clone of GPI (Myc-DDK-tagged)-Human glucose-6-phosphate isomerase (GPI), transcript variant 1 |
CNY 6,940.00 |
|
| RC230292L4 | Lenti-ORF clone of GPI (mGFP-tagged)-Human glucose-6-phosphate isomerase (GPI), transcript variant 1 |
CNY 6,940.00 |
|
| RG230292 | GPI (tGFP-tagged) - Human glucose-6-phosphate isomerase (GPI), transcript variant 1 |
CNY 5,510.00 |
|
| SC328930 | GPI (untagged)-Human glucose-6-phosphate isomerase (GPI) transcript variant 1 |
CNY 6,368.00 |
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