CPEB2 (NM_001177382) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	CPE-BP2; CPEB-2; hCPEB-2
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC230626 ORF sequence, codon optimized. Due to the complexity of NM_001177382, the ORF clone is codon optimized for mammalian Expression. The nucleotide sequence differs from the reference sequence, yet the amino acid sequence remains identical. Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCGGGACTTCGGCTTTGGCGTCCTGCAAACCGCTCCGCTCCGCTCTAGCTCCCCCGGCCCTCTGTTTT GCGGGGAGGCTTATGGGCCCTACGCCGTAGGTAGTGTGAATCCATTGCCCAGCGCAACACCTTTTGGACC GCTGAGCCCACCCCCTCTGCCAGTGACAGGTTTTCTGGAGGCAGCCTCCCCATTCTCCGTCCCCCTTGGC GGAGGTGCTGGATCCCCAGCCGCAGCAGCATCTTCCAGCAGTCCTTTTCTGGCCCACCAGCAGACAATGC AGGATGAGCTCCTTTTGGGTCTTACCCAGCAACCCGCACGACCATTGTCTGGGGCCGCCGCTACAGAGAA GCTGCCCGATCACCACCCCGGTGGTGGCACCATTGCCGGGGTGACACATTTGCTGCCAAGTCAGGATTTT AAGCCCAGCCTGCACCACCCCTCATCAAGCAGTGCCAGCAGTTGCTGCTGTTGTAGGACTTCAAGCCCCC AGGACTTCAGTAAGAGACAGCAACAGCAGCTTTCAAGCCAGAAGCGGAAGGAGTTTTCCCCGCCTCACCT GCCCCACCCCCCCGACAGTAAGCCACCCCCTCCACCCCCTCCACTGCATTGTCCTGGGCGCTTCTCCCCT CCACCCCCCCCAGCTGGACCCCTCCTGCAGCCTGCCCAGCTGGCACAGCGCCAGCAGCAGCAACCCCCTC AACAGTTCAGCTTGCTGCACCAGCAGCACCTGTCCCCTCAGGACTTTGCCCCACGCCAGCGGCCCGCTGA TCTCCCTCCTCTCCCCCAGCTGCCACCATCACCCCCAGCAGCACCCAGAAGGCGCCATGGTGGTGCCGGA TCTCCTCGAAAGACGCCCGCCGCCGGAGAGGGATCTGCCGCCGAGAGCCCCAACGCAGGGTTGGCCTCTT CTACACCGGTAAATCCTGCTCCCGGCTCCATGGAATCCCCAAACCATCCCCTCCTCAATTCCCCGAGTAA TCTTCTGCCTGGGGGCGCCCTGGGCGCGGGAGCCTTTTCCTCTCTGCAGAGTCCTGACCTGCCGCATCCT GGCGGGGGGGGGGGAGGGGGGGGTGGGGGGCCACCTGGGGGGGGAGGAGGTGGTGGTAGTGCCAGCCCTC CACCTCTCCCAGGCTTCGGAACGCCTTGGAGCGTGCAAACTGCCTCTCCTCCCCCACAGCCGCAGCAGCC ACCCCCTACCCAGCCTCAGCAACAGCCACCCCCTCCCCAGCAGCCTCCTCAGCCGCAGCCTCAGCCCCCC GGTTCATCCGCTACTACTCCAGGGGGCGGCAGCGGCGGCTCTCTCAGCGCAATGCCCCCTCCTTCCCCCG ACTCAGAGAATGGATTCTATCCTGGACTCCCCAGCAGTATGAATCCAGCATTTTTTCCATCTTTTAGTCC TGTTAGCCCCCATGGCTGTACCGGCCTCAGTGTCCCAACCTCCGGCGGCGGAGGCGGGGGCTTCGGAGGC CCCTTTTCTGCCACTGCAGTCCCTCCCCCTCCTCCCCCCGCGATGAACATCCCCCAGCAACAGCCCCCAC CACCGGCCGCTCCCCAACAGCCTCAGTCTAGGCGGAGTCCCGTCTCACCGCAGCTGCAGCAGCAGCACCA AGCAGCCGCAGCCGCTTTCCTTCAGCAGCGGAACAGTTACAATCATCATCAGCCGCTCCTTAAACAGTCC CCTTGGAGTAACCACCAATCCTCCGGTTGGGGGACGGGGTCTATGAGTTGGGGGGCGATGCACGGGAGAG ATCACAGAAGGACAGGAAACATGGGAATCCCTGGGACAATGAACCAAATAAGTCCCCTCAAAAAGCCCTT CAGCGGAAATGTTATCGCACCCCCCAAATTCACACGGTCCACACCTTCATTGACACCGAAGTCCTGGATA GAAGACAATGTTTTTAGGACTGACAACAATAGCAATACTCTGCTCCCACTGCAGGTGCGAAGCTCCCTTC AGCTGCCCGCCTGGGGATCAGACAGCCTCCAAGACTCCTGGTGCACCGCAGCCGGCACAAGCCGAATCGA TCAGGACCGCAGCCGGATGTACGACTCTCTGAATATGCACAGTCTGGAGAACAGCCTGATTGACATTATG CGAGCTGAGCACGACCCCCTTAAGGGCCGCCTCTCATATCCACATCCCGGAACCGACAATCTTCTCATGC TTAACGCCCGCAGTTATGGAAGGCGCCGAGGCAGATCTTCCCTGTTCCCTATTGATGACGGTCTTCTGGA CGACGGCCATTCAGATCAAGTAGGAGTGCTTAACAGCCCCACCTGCTACAGTGCACACCAGAACGGCGAG AGAATTGAGAGGTTCTCTCGAAAAGTTTTTGTGGGTGGGCTTCCTCCCGACATTGACGAGGATGAGATTA CAGCCAGTTTCAGGCGCTTTGGGCCCTTGGTCGTAGACTGGCCTCACAAAGCAGAATCCAAGAGCTACTT TCCTCCCAAAGGCTACGCCTTTCTCCTGTTCCAAGAAGAGTCCTCTGTCCAGGCGCTCATTGATGCTTGC ATCGAAGAAGACGGGAAGCTTTATCTGTGCGTTTCTTCACCTACTATAAAAGATAAACCAGTGCAGATTC GACCCTGGAATCTGTCCGACAGCGATTTCGTCATGGACGGTAGCCAGCCACTTGACCCAAGAAAGACCAT CTTTGTGGGCGGTGTCCCTCGCCCACTGCGAGCCGTTGAGCTCGCCATGATCATGGATCGACTCTACGGT GGAGTATGCTACGCCGGAATCGATACAGATCCGGAGCTCAAATACCCAAAGGGAGCTGGAAGGGTGGCCT TCTCCAATCAGCAATCATATATCGCTGCGATTTCCGCTCGATTCGTGCAGTTGCAACATGGCGATATCGA CAAAAGAGTGGAAGTAAAACCGTATGTGCTTGATGACCAAATGTGTGACGAGTGCCAGGGCGCTCGGTGT GGGGGTAAGTTTGCCCCCTTTTTCTGTGCCAATGTAACTTGCTTGCAGTATTACTGCGAATTCTGCTGGG CAAACATTCACTCTCGAGCCGGGAGGGAGTTCCACAAACCCTTGGTCAAGGAGGGAGCTGACCGCCCACG CCAAATCCACTTCCGATGGAAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC230626 representing NM_001177382 Red=Cloning site Green=Tags(s) MRDFGFGVLQTAPLRSSSPGPLFCGEAYGPYAVGSVNPLPSATPFGPLSPPPLPVTGFLEAASPFSVPLG GGAGSPAAAASSSSPFLAHQQTMQDELLLGLTQQPARPLSGAAATEKLPDHHPGGGTIAGVTHLLPSQDF KPSLHHPSSSSASSCCCCRTSSPQDFSKRQQQQLSSQKRKEFSPPHLPHPPDSKPPPPPPPLHCPGRFSP PPPPAGPLLQPAQLAQRQQQQPPQQFSLLHQQHLSPQDFAPRQRPADLPPLPQLPPSPPAAPRRRHGGAG SPRKTPAAGEGSAAESPNAGLASSTPVNPAPGSMESPNHPLLNSPSNLLPGGALGAGAFSSLQSPDLPHP GGGGGGGGGGPPGGGGGGGSASPPPLPGFGTPWSVQTASPPPQPQQPPPTQPQQQPPPPQQPPQPQPQPP GSSATTPGGGSGGSLSAMPPPSPDSENGFYPGLPSSMNPAFFPSFSPVSPHGCTGLSVPTSGGGGGGFGG PFSATAVPPPPPPAMNIPQQQPPPPAAPQQPQSRRSPVSPQLQQQHQAAAAAFLQQRNSYNHHQPLLKQS PWSNHQSSGWGTGSMSWGAMHGRDHRRTGNMGIPGTMNQISPLKKPFSGNVIAPPKFTRSTPSLTPKSWI EDNVFRTDNNSNTLLPLQVRSSLQLPAWGSDSLQDSWCTAAGTSRIDQDRSRMYDSLNMHSLENSLIDIM RAEHDPLKGRLSYPHPGTDNLLMLNARSYGRRRGRSSLFPIDDGLLDDGHSDQVGVLNSPTCYSAHQNGE RIERFSRKVFVGGLPPDIDEDEITASFRRFGPLVVDWPHKAESKSYFPPKGYAFLLFQEESSVQALIDAC IEEDGKLYLCVSSPTIKDKPVQIRPWNLSDSDFVMDGSQPLDPRKTIFVGGVPRPLRAVELAMIMDRLYG GVCYAGIDTDPELKYPKGAGRVAFSNQQSYIAAISARFVQLQHGDIDKRVEVKPYVLDDQMCDECQGARC GGKFAPFFCANVTCLQYYCEFCWANIHSRAGREFHKPLVKEGADRPRQIHFRWN TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001177382
ORF Size	3102 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001177382.1, NP_001170853.1
RefSeq Size	7069 bp
RefSeq ORF	3105 bp
Locus ID	132864
UniProt ID	Q7Z5Q1
MW	109.8 kDa
Gene Summary	The protein encoded by this gene is highly similar to cytoplasmic polyadenylation element binding protein (CPEB), an mRNA-binding protein that regulates cytoplasmic polyadenylation of mRNA as a trans factor in oogenesis and spermatogenesis. Studies of the similar gene in mice suggested a possible role of this protein in transcriptionally inactive haploid spermatids. Alternatively spliced transcript variants encoding distinct isoforms have been described. [provided by RefSeq, Jul 2008]

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