CLASP1 (NM_001207051) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	MAST1
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC235312 representing NM_001207051 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGCCTCGCATGGAGTCCTGCCTGGCGCAGGTGTTGCAGAAGGATGTGGGGAAACGATTGCAGGTTG GCCAAGAACTGATAGACTATTTCTCAGACAAACAGAAGTCTGCTGACCTTGAGCATGACCAGACCATGTT AGATAAACTTGTGGATGGACTTGCTACCTCTTGGGTGAACTCTAGCAATTACAAGGTGGTTCTGCTGGGC ATGGACATCCTGTCCGCCCTGGTGACCCGGCTGCAGGATCGGTTCAAGGCGCAGATCGGCACAGTGCTGC CAAGTCTAATAGACAGACTAGGAGATGCTAAAGACTCTGTGAGGGAGCAGGACCAAACTCTGCTGCTAAA GATCATGGATCAAGCTGCTAATCCCCAGTACGTATGGGACAGAATGCTTGGAGGCTTCAAACACAAGAAT TTCCGTACTCGAGAAGGCATCTGTCTCTGCCTTATAGCAACACTCAATGCCTCTGGAGCACAGACTTTAA CACTAAGCAAGATTGTGCCACATATATGCAACTTACTTGGAGATCCAAACAGCCAGGTTCGAGATGCAGC AATAAACAGCTTAGTGGAAATTTACAGACATGTAGGAGAACGTGTGAGGGCAGATCTCAGTAAAAAAGGA TTGCCACAGTCCCGGTTGAATGTAATTTTTACAAAATTTGATGAAGTCCAGAAATCTGGAAACATGATAC AATCTGCAAATGATAAAAATTTCGACGATGAAGATTCTGTGGATGGTAACAGACCTTCCTCTGCTAGTTC TACATCATCCAAGGCTCCACCAAGTTCTCGGAGAAACGTTGGAATGGGAACCACCCGCCGGCTTGGTTCA TCCACCCTTGGATCCAAGTCTTCAGCTGCAAAAGAAGGAGCTGGTGCTGTTGATGAAGAGGATTTTATTA AAGCATTTGATGATGTACCTGTAGTACAGATTTATTCCAGCCGAGACCTTGAGGAATCCATAAACAAAAT TAGGGAAATATTATCTGATGACAAGCATGATTGGGAGCAGAGAGTAAATGCTCTAAAAAAGATTAGATCT TTACTTTTGGCTGGTGCTGCTGAGTATGATAACTTCTTTCAACATTTGCGTCTTTTGGATGGAGCCTTTA AACTCTCTGCTAAGGACCTGCGGTCTCAGGTAGTGCGGGAGGCTTGTATCACGTTGGGGCATCTGTCATC AGTTCTGGGGAATAAGTTTGACCATGGAGCTGAAGCCATTATGCCAACTATCTTTAATTTAATTCCAAAC AGTGCCAAAATTATGGCCACATCTGGTGTTGTAGCTGTTAGGTTAATTATTCGGCACACACACATCCCTA GGTTAATACCTGTCATAACAAGCAACTGTACCTCTAAGTCTGTCGCAGTTAGAAGGCGCTGTTTTGAATT TTTAGATTTGCTTTTACAAGAATGGCAGACACATTCACTAGAACGACACATATCAGTATTAGCTGAAACA ATAAAGAAGGGAATACATGATGCTGATTCCGAAGCAAGAATAGAAGCCAGAAAATGTTACTGGGGTTTCC ACAGTCACTTCAGCAGAGAAGCAGAGCACTTGTACCACACCTTGGAGTCCTCCTACCAGAAAGCCCTGCA GTCCCACCTGAAGAACTCAGACAGCATAGTGTCTCTGCCTCAGTCAGACCGCTCATCTTCCAGCTCTCAA GAGAGTCTAAATCGTCCGCTGTCTGCCAAAAGAAGTCCTACTGGAAGTACCACATCTAGAGCTTCTACAG TTAGTACCAAATCTGTGTCAACGACTGGGTCCCTCCAGCGATCTCGAAGTGATATTGATGTGAACGCAGC AGCCAGTGCCAAATCCAAAGTCTCCTCATCTTCGGGCACGACGCCTTTCAGCTCTGCAGCAGCTTTGCCT CCAGGGTCATACGCATCCTTAGAGTCCAGACACATGAGGGAAGACATGGAGTACATAGGCCTGGACTCAG GTCGGATCCGCACAAGACGGCAAAGCTCTGGGAGTGCCACCAACGTCGCCTCTACACCTGATAACCGGGG CCGCAGTCGCGCTAAAGTGGTTTCACAGTCCCAGCCTGGCAGCCGGTCAAGTTCCCCAGGAAAATTGTTG GGAAGTGGTTATGGTGGACTTACTGGGGGCTCCTCACGAGGCCCACCTGTGACACCGTCTTCAGAAAAGC GAAGCAAGATTCCCAGGAGCCAGGGATGTAGCCGGGAAACAAGTCCAAACCGAATAGGATTAGATCGGTT TGGGCTTGGCCAGCCAGGAAGAATACCTGGTTCTGTGAATGCCATGAGAGTTCTGAGCACAAGTACAGAT CTTGAAGCTGCTGTTGCTGATGCTTTGCTGTTAGGAGACTCCAGGAGCAAGAAGCCTGTGAGGAGGAGAT ATGAGCCGTATGGGATGTATTCTGACGATGATGCCAACAGTGATGCCTCAAGTGTTTGCTCTGAGCGCTC ATATGGCTCCAGGAATGGTGGCATTCCCCATTATCTGCGGCAGACTGAGGATGTAGCAGAAGTTCTCAAC CACTGTGCTAGTTCAAACTGGTCAGAAAGGAAAGAAGGGCTTCTGGGCCTGCAGAACTTACTGAAGAGCC AAAGAACACTGAGTCGAGTTGAACTGAAAAGGTTGTGTGAGATCTTCACTCGGATGTTTGCTGACCCTCA TAGCAAGAGAGTTTTCAGTATGTTTTTGGAGACTCTTGTGGATTTTATAATAATTCATAAGGATGATTTA CAAGACTGGCTTTTTGTTCTTCTCACACAATTACTTAAGAAAATGGGAGCAGATTTACTTGGATCTGTGC AAGCAAAAGTTCAAAAGGCTCTAGATGTCACAAGGGACTCCTTTCCATTTGATCAACAATTTAACATTTT GATGAGATTTATTGTGGATCAAACTCAAACTCCAAACCTCAAGGTCAAAGTTGCAATCCTGAAATACATT GAGTCTCTGGCCAGACAGATGGATCCAACAGATTTTGTAAACTCTAGTGAGACAAGGCTTGCTGTTTCTA GAATCATAACCTGGACAACAGAACCAAAGAGTTCAGACGTGAGAAAGGCAGCACAGATTGTGCTAATCTC TCTGTTTGAATTGAATACTCCTGAATTTACCATGTTACTTGGTGCCTTGCCAAAAACATTCCAGGATGGT GCCACCAAACTCCTGCACAACCACCTCAAGAATTCCAGTAACACCAGTGTGGGCTCTCCAAGCAATACGA TTGGCCGGACGCCCTCCCGACACACCAGCAGCAGGACCAGCCCCCTGACCTCACCCACCAACTGTTCCCA TGGGGGTCTGTCTCCAAGCATGCTGGACTATGATACAGAGAACCTGAACTCTGAAGAAATCTATAGTTCT CTACGTGGAGTTACAGAAGCCATTGAAAAGTTTAGTTTTCGAAGCCAAGAAGATCTGAATGAGCCAATTA AACGAGATGGCAAAAAGGAGTGTGATATTGTGTCCCGCGATGGGGGCGCTGCCTCCCCTGCCACTGAGGG CCGGGGGGGTAGTGAAGTAGAAGGAGGCCGGACAGCTCTGGATAACAAGACCTCACTACTCAACACCCAG CCTCCGCGCGCCTTCCCGGGGCCGCGGGCGCGAGACTACAACCCGTACCCCTACTCAGATGCCATCAACA CCTACGACAAGACCGCCCTGAAAGAGGCTGTGTTCGATGACGACATGGAGCAGCTTCGAGACGTGCCCAT CGACCATTCTGACCTGGTGGCTGACCTTCTGAAAGAGCTGTCCAACCACAATGAGCGAGTGGAGGAACGG AAGGGAGCCCTGCTGGAGCTGCTCAAGATCACGCGGGAAGACAGCCTTGGTGTCTGGGAGGAGCACTTCA AGACCATTCTGCTCCTGCTGCTGGAGACCCTTGGAGACAAAGACCATTCAATTCGAGCACTGGCGTTAAG AGTTTTGAGGGAAATTCTGAGAAATCAACCAGCAAGATTTAAAAACTACGCCGAGCTGACGATTATGAAG ACTCTGGAAGCCCACAAAGACTCCCATAAGGAGGTGGTGAGAGCGGCTGAGGAGGCTGCGTCCACACTGG CCAGTTCCATCCACCCGGAGCAGTGCATCAAGGTGCTCTGCCCCATCATCCAGACGGCCGACTACCCCAT CAACCTTGCTGCCATCAAGATGCAGACCAAAGTCGTCGAGAGGATCGCAAAGGAGTCATTGCTGCAGCTC CTTGTCGACATCATCCCAGGCTTGCTGCAGGGTTATGACAACACCGAAAGTAGTGTGCGTAAGGCCAGCG TGTTTTGCTTAGTGGCAATTTATTCCGTAATCGGAGAAGACCTGAAACCTCACCTTGCACAGCTCACAGG GAGCAAGATGAAGCTACTAAACTTATACATAAAGAGGGCCCAGACCACCAACAGCAACAGCAGCTCCTCC TCCGATGTCTCCACGCACAGC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC235312 representing NM_001207051 Red=Cloning site Green=Tags(s) MEPRMESCLAQVLQKDVGKRLQVGQELIDYFSDKQKSADLEHDQTMLDKLVDGLATSWVNSSNYKVVLLG MDILSALVTRLQDRFKAQIGTVLPSLIDRLGDAKDSVREQDQTLLLKIMDQAANPQYVWDRMLGGFKHKN FRTREGICLCLIATLNASGAQTLTLSKIVPHICNLLGDPNSQVRDAAINSLVEIYRHVGERVRADLSKKG LPQSRLNVIFTKFDEVQKSGNMIQSANDKNFDDEDSVDGNRPSSASSTSSKAPPSSRRNVGMGTTRRLGS STLGSKSSAAKEGAGAVDEEDFIKAFDDVPVVQIYSSRDLEESINKIREILSDDKHDWEQRVNALKKIRS LLLAGAAEYDNFFQHLRLLDGAFKLSAKDLRSQVVREACITLGHLSSVLGNKFDHGAEAIMPTIFNLIPN SAKIMATSGVVAVRLIIRHTHIPRLIPVITSNCTSKSVAVRRRCFEFLDLLLQEWQTHSLERHISVLAET IKKGIHDADSEARIEARKCYWGFHSHFSREAEHLYHTLESSYQKALQSHLKNSDSIVSLPQSDRSSSSSQ ESLNRPLSAKRSPTGSTTSRASTVSTKSVSTTGSLQRSRSDIDVNAAASAKSKVSSSSGTTPFSSAAALP PGSYASLESRHMREDMEYIGLDSGRIRTRRQSSGSATNVASTPDNRGRSRAKVVSQSQPGSRSSSPGKLL GSGYGGLTGGSSRGPPVTPSSEKRSKIPRSQGCSRETSPNRIGLDRFGLGQPGRIPGSVNAMRVLSTSTD LEAAVADALLLGDSRSKKPVRRRYEPYGMYSDDDANSDASSVCSERSYGSRNGGIPHYLRQTEDVAEVLN HCASSNWSERKEGLLGLQNLLKSQRTLSRVELKRLCEIFTRMFADPHSKRVFSMFLETLVDFIIIHKDDL QDWLFVLLTQLLKKMGADLLGSVQAKVQKALDVTRDSFPFDQQFNILMRFIVDQTQTPNLKVKVAILKYI ESLARQMDPTDFVNSSETRLAVSRIITWTTEPKSSDVRKAAQIVLISLFELNTPEFTMLLGALPKTFQDG ATKLLHNHLKNSSNTSVGSPSNTIGRTPSRHTSSRTSPLTSPTNCSHGGLSPSMLDYDTENLNSEEIYSS LRGVTEAIEKFSFRSQEDLNEPIKRDGKKECDIVSRDGGAASPATEGRGGSEVEGGRTALDNKTSLLNTQ PPRAFPGPRARDYNPYPYSDAINTYDKTALKEAVFDDDMEQLRDVPIDHSDLVADLLKELSNHNERVEER KGALLELLKITREDSLGVWEEHFKTILLLLLETLGDKDHSIRALALRVLREILRNQPARFKNYAELTIMK TLEAHKDSHKEVVRAAEEAASTLASSIHPEQCIKVLCPIIQTADYPINLAAIKMQTKVVERIAKESLLQL LVDIIPGLLQGYDNTESSVRKASVFCLVAIYSVIGEDLKPHLAQLTGSKMKLLNLYIKRAQTTNSNSSSS SDVSTHS TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001207051
ORF Size	4431 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001207051.2
RefSeq Size	7935 bp
RefSeq ORF	4434 bp
Locus ID	23332
UniProt ID	Q7Z460
MW	163.5 kDa
Gene Summary	CLASPs, such as CLASP1, are nonmotor microtubule-associated proteins that interact with CLIPs (e.g., CLIP170; MIM 179838). CLASP1 is involved in the regulation of microtubule dynamics at the kinetochore and throughout the spindle (Maiato et al., 2003 [PubMed 12837247]).[supplied by OMIM, Mar 2008]

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